Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1003 and Psyr_1004 overlap by 1 nucleotides Psyr_1004 and Psyr_1005 are separated by 106 nucleotides
Psyr_1003: Psyr_1003 - nucleoside ABC transporter membrane protein, at 1,137,298 to 1,138,407
_1003
Psyr_1004: Psyr_1004 - nucleoside ABC transporter membrane protein, at 1,138,407 to 1,139,333
_1004
Psyr_1005: Psyr_1005 - OmpA/MotB, at 1,139,440 to 1,140,129
_1005
Position (kb)
1138
1139
1140 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.809 kb on - strand, within Psyr_1003 at 1137.809 kb on - strand, within Psyr_1003 at 1137.809 kb on - strand, within Psyr_1003 at 1137.809 kb on - strand, within Psyr_1003 at 1137.809 kb on - strand, within Psyr_1003 at 1137.809 kb on - strand, within Psyr_1003 at 1137.841 kb on + strand, within Psyr_1003 at 1137.841 kb on + strand, within Psyr_1003 at 1137.928 kb on + strand, within Psyr_1003 at 1137.928 kb on + strand, within Psyr_1003 at 1137.929 kb on - strand, within Psyr_1003 at 1137.930 kb on + strand, within Psyr_1003 at 1138.165 kb on + strand, within Psyr_1003 at 1138.166 kb on - strand, within Psyr_1003 at 1138.292 kb on - strand, within Psyr_1003 at 1138.292 kb on - strand, within Psyr_1003 at 1138.292 kb on - strand, within Psyr_1003 at 1138.292 kb on - strand, within Psyr_1003 at 1138.415 kb on + strand at 1138.415 kb on + strand at 1138.415 kb on + strand at 1138.415 kb on + strand at 1138.415 kb on + strand at 1138.416 kb on - strand at 1138.416 kb on - strand at 1138.416 kb on - strand at 1138.416 kb on - strand at 1138.416 kb on - strand at 1138.505 kb on - strand, within Psyr_1004 at 1138.817 kb on - strand, within Psyr_1004 at 1138.839 kb on + strand, within Psyr_1004 at 1138.839 kb on + strand, within Psyr_1004 at 1138.840 kb on - strand, within Psyr_1004 at 1138.950 kb on - strand, within Psyr_1004 at 1138.950 kb on - strand, within Psyr_1004 at 1139.205 kb on + strand, within Psyr_1004 at 1139.206 kb on - strand, within Psyr_1004 at 1139.310 kb on + strand at 1139.311 kb on - strand at 1139.516 kb on - strand, within Psyr_1005 at 1139.551 kb on - strand, within Psyr_1005 at 1139.570 kb on - strand, within Psyr_1005 at 1139.570 kb on - strand, within Psyr_1005 at 1139.570 kb on - strand, within Psyr_1005 at 1139.690 kb on - strand, within Psyr_1005 at 1139.690 kb on - strand, within Psyr_1005 at 1139.690 kb on - strand, within Psyr_1005 at 1139.692 kb on + strand, within Psyr_1005 at 1139.692 kb on + strand, within Psyr_1005 at 1139.693 kb on - strand, within Psyr_1005 at 1139.693 kb on - strand, within Psyr_1005 at 1139.750 kb on + strand, within Psyr_1005 at 1139.751 kb on - strand, within Psyr_1005 at 1139.751 kb on - strand, within Psyr_1005 at 1139.751 kb on - strand, within Psyr_1005 at 1139.836 kb on + strand, within Psyr_1005 at 1139.836 kb on + strand, within Psyr_1005 at 1139.837 kb on - strand, within Psyr_1005 at 1139.837 kb on - strand, within Psyr_1005 at 1139.977 kb on + strand, within Psyr_1005 at 1139.977 kb on + strand, within Psyr_1005 at 1139.978 kb on - strand, within Psyr_1005 at 1139.978 kb on - strand, within Psyr_1005 at 1140.124 kb on + strand at 1140.167 kb on + strand at 1140.178 kb on + strand at 1140.178 kb on + strand at 1140.179 kb on - strand at 1140.179 kb on - strand at 1140.179 kb on - strand at 1140.179 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,137,808 + Psyr_1003 0.46 +0.5 1,137,808 + Psyr_1003 0.46 -1.1 1,137,808 + Psyr_1003 0.46 +1.3 1,137,808 + Psyr_1003 0.46 +2.0 1,137,808 + Psyr_1003 0.46 +2.4 1,137,808 + Psyr_1003 0.46 +3.8 1,137,808 + Psyr_1003 0.46 +1.0 1,137,808 + Psyr_1003 0.46 +2.0 1,137,808 + Psyr_1003 0.46 -1.1 1,137,809 - Psyr_1003 0.46 +3.5 1,137,809 - Psyr_1003 0.46 -1.0 1,137,809 - Psyr_1003 0.46 +1.6 1,137,809 - Psyr_1003 0.46 -0.2 1,137,809 - Psyr_1003 0.46 +1.6 1,137,809 - Psyr_1003 0.46 +3.2 1,137,841 + Psyr_1003 0.49 -1.5 1,137,841 + Psyr_1003 0.49 -1.4 1,137,928 + Psyr_1003 0.57 +4.6 1,137,928 + Psyr_1003 0.57 -0.4 1,137,929 - Psyr_1003 0.57 +0.8 1,137,930 + Psyr_1003 0.57 +3.6 1,138,165 + Psyr_1003 0.78 -1.0 1,138,166 - Psyr_1003 0.78 -2.7 1,138,292 - Psyr_1003 0.90 -1.9 1,138,292 - Psyr_1003 0.90 -2.4 1,138,292 - Psyr_1003 0.90 -2.5 1,138,292 - Psyr_1003 0.90 +1.0 1,138,415 + +1.6 1,138,415 + -0.2 1,138,415 + +0.2 1,138,415 + -1.3 1,138,415 + -0.7 1,138,416 - -1.3 1,138,416 - -0.3 1,138,416 - -0.9 1,138,416 - -1.4 1,138,416 - -0.6 1,138,505 - Psyr_1004 0.11 -0.5 1,138,817 - Psyr_1004 0.44 +1.6 1,138,839 + Psyr_1004 0.47 +1.0 1,138,839 + Psyr_1004 0.47 +4.0 1,138,840 - Psyr_1004 0.47 +3.2 1,138,950 - Psyr_1004 0.59 -1.0 1,138,950 - Psyr_1004 0.59 -1.4 1,139,205 + Psyr_1004 0.86 +3.0 1,139,206 - Psyr_1004 0.86 -0.4 1,139,310 + -1.3 1,139,311 - +4.1 1,139,516 - Psyr_1005 0.11 -2.5 1,139,551 - Psyr_1005 0.16 -1.7 1,139,570 - Psyr_1005 0.19 -0.3 1,139,570 - Psyr_1005 0.19 -1.4 1,139,570 - Psyr_1005 0.19 -0.5 1,139,690 - Psyr_1005 0.36 -1.4 1,139,690 - Psyr_1005 0.36 -1.0 1,139,690 - Psyr_1005 0.36 -1.0 1,139,692 + Psyr_1005 0.37 +4.0 1,139,692 + Psyr_1005 0.37 +0.2 1,139,693 - Psyr_1005 0.37 -0.8 1,139,693 - Psyr_1005 0.37 +2.0 1,139,750 + Psyr_1005 0.45 +0.5 1,139,751 - Psyr_1005 0.45 -0.2 1,139,751 - Psyr_1005 0.45 +3.0 1,139,751 - Psyr_1005 0.45 -3.0 1,139,836 + Psyr_1005 0.57 +4.4 1,139,836 + Psyr_1005 0.57 +2.6 1,139,837 - Psyr_1005 0.58 +2.0 1,139,837 - Psyr_1005 0.58 -1.5 1,139,977 + Psyr_1005 0.78 +0.8 1,139,977 + Psyr_1005 0.78 -0.3 1,139,978 - Psyr_1005 0.78 -1.5 1,139,978 - Psyr_1005 0.78 -1.2 1,140,124 + +0.2 1,140,167 + +0.7 1,140,178 + -1.5 1,140,178 + +4.1 1,140,179 - +4.8 1,140,179 - -1.2 1,140,179 - -1.4 1,140,179 - +1.3
Or see this region's nucleotide sequence