Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1001 and Psyr_1002 overlap by 4 nucleotides Psyr_1002 and Psyr_1003 overlap by 8 nucleotides
Psyr_1001: Psyr_1001 - nucleoside-binding protein, at 1,134,706 to 1,135,797
_1001
Psyr_1002: Psyr_1002 - nucleoside ABC transporter ATP-binding protein, at 1,135,794 to 1,137,305
_1002
Psyr_1003: Psyr_1003 - nucleoside ABC transporter membrane protein, at 1,137,298 to 1,138,407
_1003
Position (kb)
1135
1136
1137
1138 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1134.820 kb on + strand, within Psyr_1001 at 1134.820 kb on + strand, within Psyr_1001 at 1134.820 kb on + strand, within Psyr_1001 at 1134.820 kb on + strand, within Psyr_1001 at 1134.820 kb on + strand, within Psyr_1001 at 1134.820 kb on + strand, within Psyr_1001 at 1134.820 kb on + strand, within Psyr_1001 at 1134.820 kb on + strand, within Psyr_1001 at 1134.820 kb on + strand, within Psyr_1001 at 1134.820 kb on + strand, within Psyr_1001 at 1134.820 kb on + strand, within Psyr_1001 at 1134.820 kb on + strand, within Psyr_1001 at 1134.820 kb on + strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.821 kb on - strand, within Psyr_1001 at 1134.823 kb on - strand, within Psyr_1001 at 1134.911 kb on - strand, within Psyr_1001 at 1134.911 kb on - strand, within Psyr_1001 at 1135.010 kb on - strand, within Psyr_1001 at 1135.042 kb on + strand, within Psyr_1001 at 1135.106 kb on - strand, within Psyr_1001 at 1135.113 kb on + strand, within Psyr_1001 at 1135.117 kb on + strand, within Psyr_1001 at 1135.117 kb on + strand, within Psyr_1001 at 1135.228 kb on + strand, within Psyr_1001 at 1135.279 kb on + strand, within Psyr_1001 at 1135.279 kb on + strand, within Psyr_1001 at 1135.279 kb on + strand, within Psyr_1001 at 1135.279 kb on + strand, within Psyr_1001 at 1135.279 kb on + strand, within Psyr_1001 at 1135.279 kb on + strand, within Psyr_1001 at 1135.279 kb on + strand, within Psyr_1001 at 1135.280 kb on - strand, within Psyr_1001 at 1135.280 kb on - strand, within Psyr_1001 at 1135.678 kb on + strand, within Psyr_1001 at 1135.678 kb on + strand, within Psyr_1001 at 1135.678 kb on + strand, within Psyr_1001 at 1135.678 kb on + strand, within Psyr_1001 at 1135.678 kb on + strand, within Psyr_1001 at 1135.679 kb on - strand, within Psyr_1001 at 1135.679 kb on - strand, within Psyr_1001 at 1135.959 kb on + strand, within Psyr_1002 at 1135.960 kb on - strand, within Psyr_1002 at 1136.652 kb on + strand, within Psyr_1002 at 1136.919 kb on + strand, within Psyr_1002 at 1136.919 kb on + strand, within Psyr_1002 at 1136.920 kb on - strand, within Psyr_1002 at 1136.968 kb on + strand, within Psyr_1002 at 1136.968 kb on + strand, within Psyr_1002 at 1136.969 kb on - strand, within Psyr_1002 at 1136.969 kb on - strand, within Psyr_1002 at 1136.969 kb on - strand, within Psyr_1002 at 1136.969 kb on - strand, within Psyr_1002 at 1136.969 kb on - strand, within Psyr_1002 at 1136.969 kb on - strand, within Psyr_1002 at 1137.193 kb on + strand at 1137.311 kb on + strand at 1137.312 kb on - strand at 1137.312 kb on - strand at 1137.356 kb on - strand at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.808 kb on + strand, within Psyr_1003 at 1137.809 kb on - strand, within Psyr_1003 at 1137.809 kb on - strand, within Psyr_1003 at 1137.809 kb on - strand, within Psyr_1003 at 1137.809 kb on - strand, within Psyr_1003 at 1137.809 kb on - strand, within Psyr_1003 at 1137.809 kb on - strand, within Psyr_1003 at 1137.841 kb on + strand, within Psyr_1003 at 1137.841 kb on + strand, within Psyr_1003 at 1137.928 kb on + strand, within Psyr_1003 at 1137.928 kb on + strand, within Psyr_1003 at 1137.929 kb on - strand, within Psyr_1003 at 1137.930 kb on + strand, within Psyr_1003 at 1138.165 kb on + strand, within Psyr_1003 at 1138.166 kb on - strand, within Psyr_1003 at 1138.292 kb on - strand, within Psyr_1003 at 1138.292 kb on - strand, within Psyr_1003 at 1138.292 kb on - strand, within Psyr_1003 at 1138.292 kb on - strand, within Psyr_1003
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,134,820 + Psyr_1001 0.10 +2.0 1,134,820 + Psyr_1001 0.10 -1.1 1,134,820 + Psyr_1001 0.10 +0.0 1,134,820 + Psyr_1001 0.10 -0.3 1,134,820 + Psyr_1001 0.10 +0.7 1,134,820 + Psyr_1001 0.10 +3.8 1,134,820 + Psyr_1001 0.10 -2.5 1,134,820 + Psyr_1001 0.10 -4.1 1,134,820 + Psyr_1001 0.10 +3.5 1,134,820 + Psyr_1001 0.10 -2.2 1,134,820 + Psyr_1001 0.10 -1.3 1,134,820 + Psyr_1001 0.10 +3.6 1,134,820 + Psyr_1001 0.10 +1.0 1,134,821 - Psyr_1001 0.11 -0.3 1,134,821 - Psyr_1001 0.11 -2.8 1,134,821 - Psyr_1001 0.11 +3.8 1,134,821 - Psyr_1001 0.11 +2.3 1,134,821 - Psyr_1001 0.11 -1.0 1,134,821 - Psyr_1001 0.11 +2.0 1,134,821 - Psyr_1001 0.11 -0.9 1,134,821 - Psyr_1001 0.11 +0.0 1,134,821 - Psyr_1001 0.11 -0.2 1,134,821 - Psyr_1001 0.11 +4.0 1,134,821 - Psyr_1001 0.11 +0.4 1,134,821 - Psyr_1001 0.11 +0.2 1,134,821 - Psyr_1001 0.11 -1.3 1,134,823 - Psyr_1001 0.11 -0.7 1,134,911 - Psyr_1001 0.19 -2.3 1,134,911 - Psyr_1001 0.19 +0.0 1,135,010 - Psyr_1001 0.28 +3.6 1,135,042 + Psyr_1001 0.31 -0.8 1,135,106 - Psyr_1001 0.37 +1.0 1,135,113 + Psyr_1001 0.37 +0.7 1,135,117 + Psyr_1001 0.38 +3.0 1,135,117 + Psyr_1001 0.38 +0.7 1,135,228 + Psyr_1001 0.48 +4.4 1,135,279 + Psyr_1001 0.52 -1.0 1,135,279 + Psyr_1001 0.52 +2.0 1,135,279 + Psyr_1001 0.52 +3.4 1,135,279 + Psyr_1001 0.52 -0.6 1,135,279 + Psyr_1001 0.52 +3.2 1,135,279 + Psyr_1001 0.52 -1.4 1,135,279 + Psyr_1001 0.52 -0.3 1,135,280 - Psyr_1001 0.53 +1.0 1,135,280 - Psyr_1001 0.53 -2.2 1,135,678 + Psyr_1001 0.89 +3.6 1,135,678 + Psyr_1001 0.89 +3.5 1,135,678 + Psyr_1001 0.89 +2.4 1,135,678 + Psyr_1001 0.89 -2.3 1,135,678 + Psyr_1001 0.89 +3.7 1,135,679 - Psyr_1001 0.89 -0.7 1,135,679 - Psyr_1001 0.89 -0.1 1,135,959 + Psyr_1002 0.11 +3.9 1,135,960 - Psyr_1002 0.11 +3.6 1,136,652 + Psyr_1002 0.57 -0.8 1,136,919 + Psyr_1002 0.74 -0.4 1,136,919 + Psyr_1002 0.74 -1.5 1,136,920 - Psyr_1002 0.74 -0.5 1,136,968 + Psyr_1002 0.78 +2.0 1,136,968 + Psyr_1002 0.78 -0.6 1,136,969 - Psyr_1002 0.78 -2.5 1,136,969 - Psyr_1002 0.78 -0.8 1,136,969 - Psyr_1002 0.78 -1.0 1,136,969 - Psyr_1002 0.78 -0.2 1,136,969 - Psyr_1002 0.78 +1.0 1,136,969 - Psyr_1002 0.78 +0.5 1,137,193 + +2.6 1,137,311 + +3.9 1,137,312 - -2.0 1,137,312 - -0.9 1,137,356 - -0.2 1,137,808 + Psyr_1003 0.46 +0.5 1,137,808 + Psyr_1003 0.46 -1.1 1,137,808 + Psyr_1003 0.46 +1.3 1,137,808 + Psyr_1003 0.46 +2.0 1,137,808 + Psyr_1003 0.46 +2.4 1,137,808 + Psyr_1003 0.46 +3.8 1,137,808 + Psyr_1003 0.46 +1.0 1,137,808 + Psyr_1003 0.46 +2.0 1,137,808 + Psyr_1003 0.46 -1.1 1,137,809 - Psyr_1003 0.46 +3.5 1,137,809 - Psyr_1003 0.46 -1.0 1,137,809 - Psyr_1003 0.46 +1.6 1,137,809 - Psyr_1003 0.46 -0.2 1,137,809 - Psyr_1003 0.46 +1.6 1,137,809 - Psyr_1003 0.46 +3.2 1,137,841 + Psyr_1003 0.49 -1.5 1,137,841 + Psyr_1003 0.49 -1.4 1,137,928 + Psyr_1003 0.57 +4.6 1,137,928 + Psyr_1003 0.57 -0.4 1,137,929 - Psyr_1003 0.57 +0.8 1,137,930 + Psyr_1003 0.57 +3.6 1,138,165 + Psyr_1003 0.78 -1.0 1,138,166 - Psyr_1003 0.78 -2.7 1,138,292 - Psyr_1003 0.90 -1.9 1,138,292 - Psyr_1003 0.90 -2.4 1,138,292 - Psyr_1003 0.90 -2.5 1,138,292 - Psyr_1003 0.90 +1.0
Or see this region's nucleotide sequence