Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0996 and Psyr_0997 are separated by 41 nucleotides Psyr_0997 and Psyr_0998 are separated by 10 nucleotides Psyr_0998 and Psyr_0999 overlap by 1 nucleotides
Psyr_0996: Psyr_0996 - Alpha/beta hydrolase fold protein, at 1,130,436 to 1,131,215
_0996
Psyr_0997: Psyr_0997 - Endoribonuclease L-PSP, at 1,131,257 to 1,131,640
_0997
Psyr_0998: Psyr_0998 - Isochorismatase hydrolase, at 1,131,651 to 1,132,388
_0998
Psyr_0999: Psyr_0999 - luciferase, at 1,132,388 to 1,133,470
_0999
Position (kb)
1131
1132
1133 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1130.793 kb on + strand, within Psyr_0996 at 1130.793 kb on + strand, within Psyr_0996 at 1130.793 kb on + strand, within Psyr_0996 at 1130.794 kb on - strand, within Psyr_0996 at 1130.794 kb on - strand, within Psyr_0996 at 1130.794 kb on - strand, within Psyr_0996 at 1130.794 kb on - strand, within Psyr_0996 at 1130.794 kb on - strand, within Psyr_0996 at 1130.797 kb on + strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.890 kb on - strand, within Psyr_0996 at 1131.082 kb on + strand, within Psyr_0996 at 1131.082 kb on + strand, within Psyr_0996 at 1131.082 kb on + strand, within Psyr_0996 at 1131.083 kb on - strand, within Psyr_0996 at 1131.083 kb on - strand, within Psyr_0996 at 1131.083 kb on - strand, within Psyr_0996 at 1131.355 kb on - strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.364 kb on - strand, within Psyr_0997 at 1131.364 kb on - strand, within Psyr_0997 at 1131.364 kb on - strand, within Psyr_0997 at 1131.364 kb on - strand, within Psyr_0997 at 1131.364 kb on - strand, within Psyr_0997 at 1131.364 kb on - strand, within Psyr_0997 at 1131.384 kb on + strand, within Psyr_0997 at 1131.512 kb on + strand, within Psyr_0997 at 1131.513 kb on - strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.991 kb on + strand, within Psyr_0998 at 1132.105 kb on + strand, within Psyr_0998 at 1132.105 kb on + strand, within Psyr_0998 at 1132.105 kb on + strand, within Psyr_0998 at 1132.105 kb on + strand, within Psyr_0998 at 1132.106 kb on - strand, within Psyr_0998 at 1132.106 kb on - strand, within Psyr_0998 at 1132.181 kb on + strand, within Psyr_0998 at 1132.863 kb on + strand, within Psyr_0999 at 1132.864 kb on - strand, within Psyr_0999 at 1132.864 kb on - strand, within Psyr_0999 at 1132.864 kb on - strand, within Psyr_0999 at 1133.032 kb on - strand, within Psyr_0999 at 1133.043 kb on + strand, within Psyr_0999 at 1133.044 kb on - strand, within Psyr_0999 at 1133.097 kb on + strand, within Psyr_0999 at 1133.098 kb on - strand, within Psyr_0999
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,130,793 + Psyr_0996 0.46 +4.1 1,130,793 + Psyr_0996 0.46 -0.2 1,130,793 + Psyr_0996 0.46 -0.5 1,130,794 - Psyr_0996 0.46 -1.3 1,130,794 - Psyr_0996 0.46 +0.0 1,130,794 - Psyr_0996 0.46 -1.4 1,130,794 - Psyr_0996 0.46 -1.7 1,130,794 - Psyr_0996 0.46 -0.3 1,130,797 + Psyr_0996 0.46 -1.0 1,130,798 - Psyr_0996 0.46 +1.6 1,130,798 - Psyr_0996 0.46 +2.8 1,130,798 - Psyr_0996 0.46 +1.8 1,130,798 - Psyr_0996 0.46 +4.2 1,130,798 - Psyr_0996 0.46 -1.5 1,130,798 - Psyr_0996 0.46 -0.1 1,130,798 - Psyr_0996 0.46 +1.0 1,130,890 - Psyr_0996 0.58 +1.0 1,131,082 + Psyr_0996 0.83 +2.2 1,131,082 + Psyr_0996 0.83 -0.8 1,131,082 + Psyr_0996 0.83 -1.4 1,131,083 - Psyr_0996 0.83 -1.3 1,131,083 - Psyr_0996 0.83 +1.0 1,131,083 - Psyr_0996 0.83 +0.0 1,131,355 - Psyr_0997 0.26 +2.0 1,131,363 + Psyr_0997 0.28 +2.0 1,131,363 + Psyr_0997 0.28 -1.3 1,131,363 + Psyr_0997 0.28 +1.3 1,131,363 + Psyr_0997 0.28 +1.6 1,131,363 + Psyr_0997 0.28 +0.0 1,131,363 + Psyr_0997 0.28 -2.3 1,131,363 + Psyr_0997 0.28 -0.8 1,131,363 + Psyr_0997 0.28 +2.6 1,131,363 + Psyr_0997 0.28 -2.7 1,131,364 - Psyr_0997 0.28 +1.6 1,131,364 - Psyr_0997 0.28 -1.5 1,131,364 - Psyr_0997 0.28 +2.6 1,131,364 - Psyr_0997 0.28 +2.0 1,131,364 - Psyr_0997 0.28 +3.4 1,131,364 - Psyr_0997 0.28 +0.4 1,131,384 + Psyr_0997 0.33 +1.6 1,131,512 + Psyr_0997 0.66 -2.1 1,131,513 - Psyr_0997 0.67 +0.3 1,131,555 + Psyr_0997 0.78 +0.0 1,131,555 + Psyr_0997 0.78 -0.9 1,131,555 + Psyr_0997 0.78 -2.2 1,131,555 + Psyr_0997 0.78 -2.4 1,131,555 + Psyr_0997 0.78 -0.4 1,131,555 + Psyr_0997 0.78 -2.0 1,131,555 + Psyr_0997 0.78 +4.2 1,131,555 + Psyr_0997 0.78 -1.0 1,131,555 + Psyr_0997 0.78 -2.9 1,131,555 + Psyr_0997 0.78 +1.4 1,131,555 + Psyr_0997 0.78 -0.6 1,131,555 + Psyr_0997 0.78 +1.0 1,131,556 - Psyr_0997 0.78 +2.8 1,131,556 - Psyr_0997 0.78 +0.0 1,131,556 - Psyr_0997 0.78 -4.5 1,131,556 - Psyr_0997 0.78 +1.0 1,131,556 - Psyr_0997 0.78 -0.5 1,131,556 - Psyr_0997 0.78 +2.8 1,131,556 - Psyr_0997 0.78 -1.8 1,131,556 - Psyr_0997 0.78 +2.6 1,131,556 - Psyr_0997 0.78 +1.3 1,131,991 + Psyr_0998 0.46 +4.5 1,132,105 + Psyr_0998 0.62 +2.0 1,132,105 + Psyr_0998 0.62 +3.4 1,132,105 + Psyr_0998 0.62 -3.5 1,132,105 + Psyr_0998 0.62 +2.8 1,132,106 - Psyr_0998 0.62 +4.4 1,132,106 - Psyr_0998 0.62 -1.0 1,132,181 + Psyr_0998 0.72 -1.1 1,132,863 + Psyr_0999 0.44 +0.5 1,132,864 - Psyr_0999 0.44 +0.2 1,132,864 - Psyr_0999 0.44 -0.6 1,132,864 - Psyr_0999 0.44 -0.1 1,133,032 - Psyr_0999 0.59 -0.2 1,133,043 + Psyr_0999 0.60 -4.2 1,133,044 - Psyr_0999 0.61 +0.2 1,133,097 + Psyr_0999 0.65 -0.2 1,133,098 - Psyr_0999 0.66 +2.6
Or see this region's nucleotide sequence