Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0977 and Psyr_0978 are separated by 228 nucleotides Psyr_0978 and Psyr_0979 are separated by 75 nucleotides Psyr_0979 and Psyr_0980 are separated by 297 nucleotides
Psyr_0977: Psyr_0977 - hypothetical protein, at 1,110,591 to 1,110,824
_0977
Psyr_0978: Psyr_0978 - lipoprotein, putative, at 1,111,053 to 1,111,634
_0978
Psyr_0979: Psyr_0979 - conserved hypothetical protein, at 1,111,710 to 1,111,916
_0979
Psyr_0980: Psyr_0980 - Phospholipid/glycerol acyltransferase, at 1,112,214 to 1,113,380
_0980
Position (kb)
1111
1112 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1110.215 kb on + strand at 1110.215 kb on + strand at 1110.215 kb on + strand at 1110.216 kb on - strand at 1110.216 kb on - strand at 1110.225 kb on + strand at 1110.225 kb on + strand at 1110.225 kb on + strand at 1110.229 kb on + strand at 1110.229 kb on + strand at 1110.229 kb on + strand at 1110.229 kb on + strand at 1110.230 kb on - strand at 1110.459 kb on + strand at 1110.459 kb on + strand at 1110.471 kb on - strand at 1110.473 kb on + strand at 1110.474 kb on - strand at 1110.483 kb on - strand at 1110.517 kb on + strand at 1110.517 kb on + strand at 1110.518 kb on - strand at 1110.518 kb on - strand at 1110.519 kb on + strand at 1110.534 kb on + strand at 1110.534 kb on + strand at 1110.534 kb on + strand at 1110.534 kb on + strand at 1110.534 kb on + strand at 1110.535 kb on - strand at 1110.535 kb on - strand at 1110.535 kb on - strand at 1110.535 kb on - strand at 1110.535 kb on - strand at 1110.536 kb on + strand at 1110.537 kb on - strand at 1110.537 kb on - strand at 1110.567 kb on - strand at 1110.567 kb on - strand at 1110.577 kb on + strand at 1110.578 kb on - strand at 1110.845 kb on - strand at 1110.899 kb on + strand at 1110.899 kb on + strand at 1110.900 kb on - strand at 1111.000 kb on + strand at 1111.001 kb on - strand at 1111.001 kb on - strand at 1111.011 kb on - strand at 1111.012 kb on + strand at 1111.012 kb on + strand at 1111.012 kb on + strand at 1111.013 kb on - strand at 1111.013 kb on - strand at 1111.013 kb on - strand at 1111.013 kb on - strand at 1111.013 kb on - strand at 1111.087 kb on + strand at 1111.088 kb on - strand at 1111.249 kb on - strand, within Psyr_0978 at 1111.399 kb on - strand, within Psyr_0978 at 1111.399 kb on - strand, within Psyr_0978 at 1111.422 kb on + strand, within Psyr_0978 at 1111.422 kb on + strand, within Psyr_0978 at 1111.422 kb on + strand, within Psyr_0978 at 1111.423 kb on - strand, within Psyr_0978 at 1111.423 kb on - strand, within Psyr_0978 at 1111.423 kb on - strand, within Psyr_0978 at 1111.423 kb on - strand, within Psyr_0978 at 1111.423 kb on - strand, within Psyr_0978 at 1111.485 kb on + strand, within Psyr_0978 at 1111.485 kb on + strand, within Psyr_0978 at 1111.485 kb on + strand, within Psyr_0978 at 1111.485 kb on + strand, within Psyr_0978 at 1111.485 kb on + strand, within Psyr_0978 at 1111.485 kb on + strand, within Psyr_0978 at 1111.485 kb on + strand, within Psyr_0978 at 1111.486 kb on - strand, within Psyr_0978 at 1111.486 kb on - strand, within Psyr_0978 at 1111.486 kb on - strand, within Psyr_0978 at 1111.486 kb on - strand, within Psyr_0978 at 1111.709 kb on - strand at 1111.709 kb on - strand at 1111.726 kb on + strand at 1111.726 kb on + strand at 1111.804 kb on + strand, within Psyr_0979 at 1111.805 kb on - strand, within Psyr_0979 at 1111.885 kb on + strand, within Psyr_0979 at 1111.885 kb on + strand, within Psyr_0979 at 1111.886 kb on - strand, within Psyr_0979 at 1111.886 kb on - strand, within Psyr_0979 at 1111.975 kb on + strand at 1111.975 kb on + strand at 1111.975 kb on + strand at 1111.975 kb on + strand at 1111.975 kb on + strand at 1111.976 kb on - strand at 1111.976 kb on - strand at 1111.976 kb on - strand at 1111.976 kb on - strand at 1112.031 kb on - strand at 1112.076 kb on + strand at 1112.076 kb on + strand at 1112.077 kb on - strand at 1112.077 kb on - strand at 1112.077 kb on - strand at 1112.077 kb on - strand at 1112.077 kb on - strand at 1112.199 kb on + strand at 1112.199 kb on + strand at 1112.199 kb on + strand at 1112.200 kb on - strand at 1112.200 kb on - strand at 1112.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 1,110,215 + +0.0 1,110,215 + +0.0 1,110,215 + -1.7 1,110,216 - +3.2 1,110,216 - +1.0 1,110,225 + +2.4 1,110,225 + -1.7 1,110,225 + +0.7 1,110,229 + +1.6 1,110,229 + +1.0 1,110,229 + -2.0 1,110,229 + +2.4 1,110,230 - -1.3 1,110,459 + -0.5 1,110,459 + -2.3 1,110,471 - +0.4 1,110,473 + -2.0 1,110,474 - -1.0 1,110,483 - -0.2 1,110,517 + -0.6 1,110,517 + -1.5 1,110,518 - +4.1 1,110,518 - -0.3 1,110,519 + -3.0 1,110,534 + +3.2 1,110,534 + +0.0 1,110,534 + +1.6 1,110,534 + +2.0 1,110,534 + +3.0 1,110,535 - +0.5 1,110,535 - +3.7 1,110,535 - +0.6 1,110,535 - +0.4 1,110,535 - +3.5 1,110,536 + +2.4 1,110,537 - -2.7 1,110,537 - +2.0 1,110,567 - +0.4 1,110,567 - +0.2 1,110,577 + -2.9 1,110,578 - -5.0 1,110,845 - +1.6 1,110,899 + -0.2 1,110,899 + -1.8 1,110,900 - +2.4 1,111,000 + +4.1 1,111,001 - -0.5 1,111,001 - +0.0 1,111,011 - +0.5 1,111,012 + +2.4 1,111,012 + -0.1 1,111,012 + -0.5 1,111,013 - -1.0 1,111,013 - +0.0 1,111,013 - +0.2 1,111,013 - -2.0 1,111,013 - -1.8 1,111,087 + +1.3 1,111,088 - -2.8 1,111,249 - Psyr_0978 0.34 -1.3 1,111,399 - Psyr_0978 0.59 -2.2 1,111,399 - Psyr_0978 0.59 -4.8 1,111,422 + Psyr_0978 0.63 -1.5 1,111,422 + Psyr_0978 0.63 +3.0 1,111,422 + Psyr_0978 0.63 +1.0 1,111,423 - Psyr_0978 0.64 -1.6 1,111,423 - Psyr_0978 0.64 -3.0 1,111,423 - Psyr_0978 0.64 -2.8 1,111,423 - Psyr_0978 0.64 -3.5 1,111,423 - Psyr_0978 0.64 -2.3 1,111,485 + Psyr_0978 0.74 -1.5 1,111,485 + Psyr_0978 0.74 +3.0 1,111,485 + Psyr_0978 0.74 -4.1 1,111,485 + Psyr_0978 0.74 -2.9 1,111,485 + Psyr_0978 0.74 -0.3 1,111,485 + Psyr_0978 0.74 -2.8 1,111,485 + Psyr_0978 0.74 +1.0 1,111,486 - Psyr_0978 0.74 -1.9 1,111,486 - Psyr_0978 0.74 +2.0 1,111,486 - Psyr_0978 0.74 -2.0 1,111,486 - Psyr_0978 0.74 -1.3 1,111,709 - -0.3 1,111,709 - +3.6 1,111,726 + +3.6 1,111,726 + -0.7 1,111,804 + Psyr_0979 0.45 +0.3 1,111,805 - Psyr_0979 0.46 +4.1 1,111,885 + Psyr_0979 0.85 -0.5 1,111,885 + Psyr_0979 0.85 +1.0 1,111,886 - Psyr_0979 0.85 -1.1 1,111,886 - Psyr_0979 0.85 -0.8 1,111,975 + -1.0 1,111,975 + +3.5 1,111,975 + +0.9 1,111,975 + -0.8 1,111,975 + +4.4 1,111,976 - +2.6 1,111,976 - +3.6 1,111,976 - -0.6 1,111,976 - +1.3 1,112,031 - +0.0 1,112,076 + +0.3 1,112,076 + +3.6 1,112,077 - -1.1 1,112,077 - -1.5 1,112,077 - -0.1 1,112,077 - -0.6 1,112,077 - -0.9 1,112,199 + +0.9 1,112,199 + -1.5 1,112,199 + -1.0 1,112,200 - -1.6 1,112,200 - -2.5 1,112,200 - -1.7
Or see this region's nucleotide sequence