Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0923

Experiment: KB with Acridine orange 62.5 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0921 and Psyr_0922 are separated by 9 nucleotidesPsyr_0922 and Psyr_0923 overlap by 4 nucleotidesPsyr_0923 and Psyr_0924 are separated by 109 nucleotidesPsyr_0924 and Psyr_0925 are separated by 8 nucleotides Psyr_0921: Psyr_0921 - GtrA-like protein, at 1,048,744 to 1,049,232 _0921 Psyr_0922: Psyr_0922 - Glycosyl transferase, family 2, at 1,049,242 to 1,050,096 _0922 Psyr_0923: Psyr_0923 - hypothetical protein, at 1,050,093 to 1,051,553 _0923 Psyr_0924: Psyr_0924 - Glucose-1-phosphate thymidylyltransferase, at 1,051,663 to 1,052,544 _0924 Psyr_0925: Psyr_0925 - dTDP-4-dehydrorhamnose reductase, at 1,052,553 to 1,053,458 _0925 Position (kb) 1050 1051 1052Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 1050.122 kb on + strandat 1050.130 kb on + strandat 1050.136 kb on + strandat 1050.152 kb on + strandat 1050.153 kb on - strandat 1050.153 kb on - strandat 1050.302 kb on - strand, within Psyr_0923at 1050.489 kb on - strand, within Psyr_0923at 1050.489 kb on - strand, within Psyr_0923at 1050.579 kb on - strand, within Psyr_0923at 1050.610 kb on + strand, within Psyr_0923at 1050.611 kb on - strand, within Psyr_0923at 1050.625 kb on - strand, within Psyr_0923at 1050.629 kb on + strand, within Psyr_0923at 1050.651 kb on + strand, within Psyr_0923at 1050.822 kb on - strand, within Psyr_0923at 1050.893 kb on + strand, within Psyr_0923at 1051.041 kb on - strand, within Psyr_0923at 1051.097 kb on + strand, within Psyr_0923at 1051.097 kb on + strand, within Psyr_0923at 1051.098 kb on - strand, within Psyr_0923at 1051.101 kb on + strand, within Psyr_0923at 1051.101 kb on + strand, within Psyr_0923at 1051.141 kb on + strand, within Psyr_0923at 1051.142 kb on - strandat 1051.173 kb on + strand, within Psyr_0923at 1051.234 kb on + strand, within Psyr_0923at 1051.235 kb on - strand, within Psyr_0923at 1051.235 kb on - strand, within Psyr_0923at 1051.235 kb on - strand, within Psyr_0923at 1051.326 kb on + strand, within Psyr_0923at 1051.326 kb on + strand, within Psyr_0923at 1051.326 kb on + strand, within Psyr_0923at 1051.327 kb on - strand, within Psyr_0923at 1051.452 kb on - strandat 1051.502 kb on + strandat 1051.502 kb on + strandat 1051.510 kb on + strandat 1051.510 kb on + strandat 1051.510 kb on + strandat 1051.563 kb on + strandat 1051.563 kb on + strandat 1051.563 kb on + strandat 1051.563 kb on + strandat 1051.563 kb on + strandat 1051.563 kb on + strandat 1051.563 kb on + strandat 1051.563 kb on + strandat 1051.563 kb on + strandat 1051.563 kb on + strandat 1051.564 kb on - strandat 1051.564 kb on - strandat 1051.564 kb on - strandat 1051.564 kb on - strandat 1051.564 kb on - strandat 1051.564 kb on - strandat 1051.564 kb on - strandat 1051.564 kb on - strandat 1051.904 kb on - strand, within Psyr_0924at 1052.013 kb on - strand, within Psyr_0924at 1052.037 kb on - strand, within Psyr_0924

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml
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1,050,122 + +3.1
1,050,130 + +1.1
1,050,136 + -1.8
1,050,152 + +0.1
1,050,153 - +1.1
1,050,153 - +0.1
1,050,302 - Psyr_0923 0.14 +0.1
1,050,489 - Psyr_0923 0.27 +2.4
1,050,489 - Psyr_0923 0.27 +0.1
1,050,579 - Psyr_0923 0.33 +0.1
1,050,610 + Psyr_0923 0.35 -3.8
1,050,611 - Psyr_0923 0.35 +1.1
1,050,625 - Psyr_0923 0.36 -3.4
1,050,629 + Psyr_0923 0.37 -4.3
1,050,651 + Psyr_0923 0.38 +0.1
1,050,822 - Psyr_0923 0.50 +0.1
1,050,893 + Psyr_0923 0.55 +2.4
1,051,041 - Psyr_0923 0.65 +1.7
1,051,097 + Psyr_0923 0.69 +1.1
1,051,097 + Psyr_0923 0.69 -3.4
1,051,098 - Psyr_0923 0.69 +1.7
1,051,101 + Psyr_0923 0.69 -3.8
1,051,101 + Psyr_0923 0.69 +2.9
1,051,141 + Psyr_0923 0.72 +0.1
1,051,142 - +1.1
1,051,173 + Psyr_0923 0.74 +2.1
1,051,234 + Psyr_0923 0.78 -2.4
1,051,235 - Psyr_0923 0.78 +2.1
1,051,235 - Psyr_0923 0.78 -4.7
1,051,235 - Psyr_0923 0.78 +1.7
1,051,326 + Psyr_0923 0.84 -1.4
1,051,326 + Psyr_0923 0.84 -2.1
1,051,326 + Psyr_0923 0.84 +1.5
1,051,327 - Psyr_0923 0.84 +3.1
1,051,452 - +0.1
1,051,502 + +0.1
1,051,502 + +1.1
1,051,510 + +3.8
1,051,510 + +0.6
1,051,510 + +0.1
1,051,563 + +3.8
1,051,563 + +0.5
1,051,563 + -0.6
1,051,563 + -1.8
1,051,563 + -0.3
1,051,563 + +4.7
1,051,563 + +2.6
1,051,563 + -3.1
1,051,563 + -1.6
1,051,563 + -0.8
1,051,564 - +1.1
1,051,564 - -1.7
1,051,564 - +0.1
1,051,564 - -1.4
1,051,564 - +1.7
1,051,564 - -1.7
1,051,564 - -3.8
1,051,564 - -2.1
1,051,904 - Psyr_0924 0.27 -4.7
1,052,013 - Psyr_0924 0.40 -3.4
1,052,037 - Psyr_0924 0.42 +0.1

Or see this region's nucleotide sequence