Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0830 and Psyr_0831 are separated by 626 nucleotides Psyr_0831 and Psyr_0832 overlap by 29 nucleotides
Psyr_0830: Psyr_0830 - Poly(A) polymerase, at 939,218 to 940,693
_0830
Psyr_0831: Psyr_0831 - Two-component response regulator CbrB, at 941,320 to 942,804
_0831
Psyr_0832: Psyr_0832 - Two-component sensor kinase CbrA, at 942,776 to 945,730
_0832
Position (kb)
941
942
943 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 940.565 kb on - strand at 940.565 kb on - strand at 940.565 kb on - strand at 940.565 kb on - strand at 940.688 kb on + strand at 940.689 kb on - strand at 940.689 kb on - strand at 940.695 kb on + strand at 940.696 kb on - strand at 940.711 kb on + strand at 940.711 kb on + strand at 940.712 kb on - strand at 940.712 kb on - strand at 940.712 kb on - strand at 940.763 kb on + strand at 940.763 kb on + strand at 940.764 kb on - strand at 940.802 kb on + strand at 940.805 kb on + strand at 940.806 kb on - strand at 940.957 kb on + strand at 940.957 kb on + strand at 940.957 kb on + strand at 940.957 kb on + strand at 940.957 kb on + strand at 940.957 kb on + strand at 940.957 kb on + strand at 940.957 kb on + strand at 940.958 kb on - strand at 940.958 kb on - strand at 940.958 kb on - strand at 940.963 kb on + strand at 940.963 kb on + strand at 940.964 kb on - strand at 941.006 kb on + strand at 941.006 kb on + strand at 941.006 kb on + strand at 941.006 kb on + strand at 941.006 kb on + strand at 941.006 kb on + strand at 941.006 kb on + strand at 941.006 kb on + strand at 941.007 kb on - strand at 941.007 kb on - strand at 941.033 kb on - strand at 941.033 kb on - strand at 941.049 kb on + strand at 941.049 kb on + strand at 941.088 kb on + strand at 941.089 kb on - strand at 941.103 kb on - strand at 941.124 kb on - strand at 941.124 kb on - strand at 941.132 kb on + strand at 941.132 kb on + strand at 941.132 kb on + strand at 941.132 kb on + strand at 941.132 kb on + strand at 941.133 kb on - strand at 941.133 kb on - strand at 941.133 kb on - strand at 941.135 kb on - strand at 941.135 kb on - strand at 941.135 kb on - strand at 941.135 kb on - strand at 941.234 kb on + strand at 941.234 kb on + strand at 941.270 kb on - strand at 941.321 kb on + strand at 941.321 kb on + strand at 941.322 kb on - strand at 941.322 kb on - strand at 941.322 kb on - strand at 941.322 kb on - strand at 941.544 kb on - strand, within Psyr_0831 at 941.657 kb on - strand, within Psyr_0831 at 941.657 kb on - strand, within Psyr_0831 at 941.676 kb on - strand, within Psyr_0831 at 941.831 kb on + strand, within Psyr_0831 at 941.831 kb on + strand, within Psyr_0831 at 941.832 kb on - strand, within Psyr_0831 at 942.903 kb on + strand at 942.904 kb on - strand at 943.170 kb on + strand, within Psyr_0832 at 943.171 kb on - strand, within Psyr_0832 at 943.178 kb on + strand, within Psyr_0832 at 943.178 kb on + strand, within Psyr_0832 at 943.179 kb on - strand, within Psyr_0832 at 943.179 kb on - strand, within Psyr_0832 at 943.216 kb on + strand, within Psyr_0832 at 943.217 kb on - strand, within Psyr_0832 at 943.424 kb on - strand, within Psyr_0832
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 940,565 - +1.7 940,565 - +0.5 940,565 - +1.1 940,565 - -2.4 940,688 + -2.7 940,689 - +0.9 940,689 - -0.3 940,695 + +4.8 940,696 - +1.7 940,711 + -0.1 940,711 + +0.1 940,712 - -1.4 940,712 - -1.6 940,712 - -1.5 940,763 + +3.6 940,763 + +1.7 940,764 - +3.6 940,802 + -1.1 940,805 + +0.1 940,806 - +1.7 940,957 + -1.4 940,957 + +1.7 940,957 + -4.4 940,957 + +0.1 940,957 + +1.1 940,957 + +2.9 940,957 + -1.1 940,957 + +2.5 940,958 - +1.1 940,958 - +1.1 940,958 - +1.1 940,963 + +2.5 940,963 + +0.1 940,964 - -4.3 941,006 + +0.7 941,006 + -1.6 941,006 + +1.1 941,006 + +0.1 941,006 + +2.9 941,006 + +1.1 941,006 + -1.1 941,006 + -0.5 941,007 - -1.4 941,007 - -1.5 941,033 - +2.1 941,033 - -0.2 941,049 + -0.4 941,049 + +0.8 941,088 + -0.6 941,089 - +3.7 941,103 - +3.3 941,124 - +3.7 941,124 - -1.4 941,132 + -0.9 941,132 + -1.6 941,132 + -1.0 941,132 + -1.5 941,132 + +3.5 941,133 - +0.6 941,133 - -0.4 941,133 - -2.4 941,135 - -1.0 941,135 - -0.4 941,135 - +2.9 941,135 - +1.7 941,234 + -1.3 941,234 + -1.2 941,270 - +2.5 941,321 + -2.1 941,321 + -2.1 941,322 - +1.7 941,322 - +1.1 941,322 - +3.3 941,322 - +1.9 941,544 - Psyr_0831 0.15 -3.4 941,657 - Psyr_0831 0.23 +2.1 941,657 - Psyr_0831 0.23 +0.1 941,676 - Psyr_0831 0.24 +1.1 941,831 + Psyr_0831 0.34 +1.7 941,831 + Psyr_0831 0.34 -2.2 941,832 - Psyr_0831 0.34 +0.1 942,903 + +0.1 942,904 - +2.1 943,170 + Psyr_0832 0.13 +0.1 943,171 - Psyr_0832 0.13 +0.1 943,178 + Psyr_0832 0.14 -0.9 943,178 + Psyr_0832 0.14 +0.1 943,179 - Psyr_0832 0.14 +0.1 943,179 - Psyr_0832 0.14 +3.3 943,216 + Psyr_0832 0.15 -1.3 943,217 - Psyr_0832 0.15 -3.0 943,424 - Psyr_0832 0.22 +2.1
Or see this region's nucleotide sequence