Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0825 and Psyr_0826 are separated by 383 nucleotides Psyr_0826 and Psyr_0827 are separated by 253 nucleotides
Psyr_0825: Psyr_0825 - conserved hypothetical protein, at 933,674 to 934,540
_0825
Psyr_0826: Psyr_0826 - glucose-6-phosphate isomerase, at 934,924 to 936,588
_0826
Psyr_0827: Psyr_0827 - pantothenate synthetase, at 936,842 to 937,693
_0827
Position (kb) 934
935
936
937 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 933.963 kb on + strand, within Psyr_0825 at 933.963 kb on + strand, within Psyr_0825 at 933.963 kb on + strand, within Psyr_0825 at 933.964 kb on - strand, within Psyr_0825 at 934.038 kb on + strand, within Psyr_0825 at 934.038 kb on + strand, within Psyr_0825 at 934.041 kb on + strand, within Psyr_0825 at 934.041 kb on + strand, within Psyr_0825 at 934.041 kb on + strand, within Psyr_0825 at 934.042 kb on - strand, within Psyr_0825 at 934.080 kb on + strand, within Psyr_0825 at 934.118 kb on + strand, within Psyr_0825 at 934.119 kb on - strand, within Psyr_0825 at 934.158 kb on + strand, within Psyr_0825 at 934.241 kb on - strand, within Psyr_0825 at 934.248 kb on + strand, within Psyr_0825 at 934.248 kb on + strand, within Psyr_0825 at 934.248 kb on + strand, within Psyr_0825 at 934.249 kb on - strand, within Psyr_0825 at 934.568 kb on + strand at 934.568 kb on + strand at 934.569 kb on - strand at 934.569 kb on - strand at 934.632 kb on + strand at 934.649 kb on + strand at 934.649 kb on + strand at 934.649 kb on + strand at 934.649 kb on + strand at 934.650 kb on - strand at 934.650 kb on - strand at 934.662 kb on - strand at 934.662 kb on - strand at 934.662 kb on - strand at 934.688 kb on + strand at 934.688 kb on + strand at 934.688 kb on + strand at 934.688 kb on + strand at 934.688 kb on + strand at 934.689 kb on - strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.701 kb on + strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.702 kb on - strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.718 kb on + strand at 934.719 kb on - strand at 934.719 kb on - strand at 934.719 kb on - strand at 934.719 kb on - strand at 934.802 kb on + strand at 934.802 kb on + strand at 934.802 kb on + strand at 934.802 kb on + strand at 934.802 kb on + strand at 934.845 kb on + strand at 934.845 kb on + strand at 934.846 kb on - strand at 934.949 kb on + strand at 934.949 kb on + strand at 934.950 kb on - strand at 934.950 kb on - strand at 935.016 kb on - strand at 935.016 kb on - strand at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.111 kb on + strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.112 kb on - strand, within Psyr_0826 at 935.414 kb on + strand, within Psyr_0826 at 935.414 kb on + strand, within Psyr_0826 at 935.415 kb on - strand, within Psyr_0826 at 935.481 kb on + strand, within Psyr_0826 at 935.555 kb on + strand, within Psyr_0826 at 935.684 kb on + strand, within Psyr_0826 at 935.685 kb on - strand, within Psyr_0826 at 935.685 kb on - strand, within Psyr_0826 at 935.791 kb on - strand, within Psyr_0826 at 935.791 kb on - strand, within Psyr_0826 at 935.900 kb on + strand, within Psyr_0826 at 935.943 kb on + strand, within Psyr_0826 at 936.039 kb on - strand, within Psyr_0826 at 936.039 kb on - strand, within Psyr_0826 at 936.221 kb on - strand, within Psyr_0826 at 936.333 kb on - strand, within Psyr_0826 at 936.333 kb on - strand, within Psyr_0826 at 936.581 kb on + strand at 936.582 kb on - strand at 936.605 kb on + strand at 936.605 kb on + strand at 936.687 kb on - strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.762 kb on + strand at 936.817 kb on + strand at 936.817 kb on + strand at 936.817 kb on + strand at 936.817 kb on + strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.970 kb on - strand, within Psyr_0827 at 937.080 kb on + strand, within Psyr_0827 at 937.081 kb on - strand, within Psyr_0827 at 937.161 kb on - strand, within Psyr_0827 at 937.398 kb on + strand, within Psyr_0827 at 937.482 kb on + strand, within Psyr_0827 at 937.482 kb on + strand, within Psyr_0827 at 937.482 kb on + strand, within Psyr_0827 at 937.483 kb on - strand, within Psyr_0827 at 937.531 kb on + strand, within Psyr_0827
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 933,963 + Psyr_0825 0.33 +2.7 933,963 + Psyr_0825 0.33 -1.1 933,963 + Psyr_0825 0.33 +0.1 933,964 - Psyr_0825 0.33 +3.7 934,038 + Psyr_0825 0.42 -0.4 934,038 + Psyr_0825 0.42 -1.4 934,041 + Psyr_0825 0.42 +0.1 934,041 + Psyr_0825 0.42 +0.1 934,041 + Psyr_0825 0.42 +4.1 934,042 - Psyr_0825 0.42 -2.2 934,080 + Psyr_0825 0.47 +2.0 934,118 + Psyr_0825 0.51 -1.8 934,119 - Psyr_0825 0.51 +0.8 934,158 + Psyr_0825 0.56 +1.7 934,241 - Psyr_0825 0.65 -0.1 934,248 + Psyr_0825 0.66 -1.4 934,248 + Psyr_0825 0.66 +0.7 934,248 + Psyr_0825 0.66 -0.3 934,249 - Psyr_0825 0.66 -0.3 934,568 + -2.2 934,568 + +1.1 934,569 - -0.5 934,569 - +3.6 934,632 + +4.1 934,649 + -1.6 934,649 + -0.2 934,649 + +2.9 934,649 + +2.9 934,650 - -1.5 934,650 - +3.6 934,662 - +1.1 934,662 - +1.1 934,662 - -2.0 934,688 + -2.5 934,688 + -0.8 934,688 + +0.1 934,688 + +2.7 934,688 + -0.1 934,689 - -3.3 934,701 + +3.1 934,701 + +0.1 934,701 + -0.9 934,701 + +3.6 934,701 + -1.7 934,701 + +2.1 934,701 + +2.1 934,701 + +0.2 934,701 + +4.0 934,701 + -0.9 934,702 - +0.8 934,702 - +1.1 934,702 - -1.5 934,702 - -1.9 934,702 - -1.9 934,702 - -1.3 934,702 - -0.5 934,702 - +3.6 934,702 - -0.7 934,718 + +3.3 934,718 + -1.3 934,718 + -0.6 934,718 + -2.2 934,718 + -1.7 934,718 + -0.6 934,718 + -0.1 934,718 + +3.6 934,718 + -1.0 934,718 + +1.1 934,718 + -2.4 934,718 + -2.3 934,718 + +1.1 934,718 + -2.0 934,719 - +4.1 934,719 - -0.9 934,719 - -1.0 934,719 - -1.5 934,802 + +1.1 934,802 + +1.7 934,802 + -0.8 934,802 + +2.5 934,802 + -1.2 934,845 + -0.8 934,845 + -1.7 934,846 - +2.1 934,949 + +1.1 934,949 + +0.1 934,950 - +0.1 934,950 - +0.1 935,016 - -1.8 935,016 - +0.1 935,111 + Psyr_0826 0.11 -2.7 935,111 + Psyr_0826 0.11 -3.5 935,111 + Psyr_0826 0.11 +0.1 935,111 + Psyr_0826 0.11 -3.7 935,111 + Psyr_0826 0.11 +2.1 935,111 + Psyr_0826 0.11 +0.1 935,111 + Psyr_0826 0.11 +1.1 935,111 + Psyr_0826 0.11 -4.1 935,111 + Psyr_0826 0.11 -1.9 935,111 + Psyr_0826 0.11 -1.9 935,111 + Psyr_0826 0.11 -1.0 935,111 + Psyr_0826 0.11 +0.1 935,111 + Psyr_0826 0.11 -3.2 935,111 + Psyr_0826 0.11 -1.5 935,112 - Psyr_0826 0.11 -1.5 935,112 - Psyr_0826 0.11 -2.3 935,112 - Psyr_0826 0.11 +2.1 935,112 - Psyr_0826 0.11 +1.7 935,112 - Psyr_0826 0.11 -2.1 935,112 - Psyr_0826 0.11 +1.7 935,112 - Psyr_0826 0.11 +0.1 935,112 - Psyr_0826 0.11 +0.1 935,414 + Psyr_0826 0.29 -1.7 935,414 + Psyr_0826 0.29 -0.5 935,415 - Psyr_0826 0.29 -3.3 935,481 + Psyr_0826 0.33 -1.2 935,555 + Psyr_0826 0.38 +0.1 935,684 + Psyr_0826 0.46 -1.1 935,685 - Psyr_0826 0.46 -3.9 935,685 - Psyr_0826 0.46 -1.5 935,791 - Psyr_0826 0.52 -2.0 935,791 - Psyr_0826 0.52 +3.1 935,900 + Psyr_0826 0.59 +0.1 935,943 + Psyr_0826 0.61 -3.2 936,039 - Psyr_0826 0.67 +1.1 936,039 - Psyr_0826 0.67 -1.0 936,221 - Psyr_0826 0.78 -3.1 936,333 - Psyr_0826 0.85 +1.1 936,333 - Psyr_0826 0.85 +0.1 936,581 + -1.2 936,582 - -2.7 936,605 + -2.4 936,605 + -3.3 936,687 - +3.5 936,710 + +3.7 936,710 + -0.9 936,710 + +2.7 936,710 + +2.5 936,710 + +0.1 936,710 + +1.1 936,711 - +4.2 936,711 - -0.8 936,711 - +0.4 936,711 - -0.4 936,711 - +2.9 936,711 - +1.1 936,711 - -1.3 936,762 + -1.1 936,817 + +3.3 936,817 + +1.7 936,817 + -1.3 936,817 + -0.8 936,818 - -1.4 936,818 - +3.3 936,818 - -1.7 936,818 - +2.7 936,818 - -0.5 936,818 - +4.2 936,818 - -1.1 936,970 - Psyr_0827 0.15 -3.2 937,080 + Psyr_0827 0.28 +2.1 937,081 - Psyr_0827 0.28 +3.3 937,161 - Psyr_0827 0.37 +3.1 937,398 + Psyr_0827 0.65 -1.4 937,482 + Psyr_0827 0.75 -1.2 937,482 + Psyr_0827 0.75 -0.0 937,482 + Psyr_0827 0.75 -0.4 937,483 - Psyr_0827 0.75 -1.9 937,531 + Psyr_0827 0.81 +0.1
Or see this region's nucleotide sequence