Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0821 and Psyr_0822 overlap by 1 nucleotides Psyr_0822 and Psyr_0823 are separated by 12 nucleotides
Psyr_0821: Psyr_0821 - PTS system D-fructose-specific IIA component (F1P-forming), Frc family / Phosphocarrier protein HPr / phosphoenolpyruvate--protein phosphotransferase, at 926,782 to 929,652
_0821
Psyr_0822: Psyr_0822 - 1-phosphofructokinase, at 929,652 to 930,593
_0822
Psyr_0823: Psyr_0823 - PTS system D-fructose-specific IIB component (F1P-forming), Frc family / PTS system D-fructose-specific IIC component (F1P-forming), Frc family, at 930,606 to 932,348
_0823
Position (kb)
929
930
931 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 928.682 kb on + strand, within Psyr_0821 at 928.683 kb on - strand, within Psyr_0821 at 928.683 kb on - strand, within Psyr_0821 at 929.320 kb on + strand, within Psyr_0821 at 929.320 kb on + strand, within Psyr_0821 at 929.320 kb on + strand, within Psyr_0821 at 929.320 kb on + strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.650 kb on + strand at 929.650 kb on + strand at 929.651 kb on - strand at 929.782 kb on + strand, within Psyr_0822 at 929.783 kb on - strand, within Psyr_0822 at 930.524 kb on + strand at 930.571 kb on + strand at 930.572 kb on - strand at 930.591 kb on + strand at 930.591 kb on + strand at 930.592 kb on - strand at 930.592 kb on - strand at 930.592 kb on - strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.620 kb on - strand at 930.620 kb on - strand at 930.620 kb on - strand at 930.620 kb on - strand at 930.655 kb on + strand at 931.003 kb on + strand, within Psyr_0823 at 931.003 kb on + strand, within Psyr_0823 at 931.004 kb on - strand, within Psyr_0823 at 931.059 kb on + strand, within Psyr_0823 at 931.059 kb on + strand, within Psyr_0823 at 931.197 kb on + strand, within Psyr_0823 at 931.198 kb on - strand, within Psyr_0823 at 931.198 kb on - strand, within Psyr_0823 at 931.326 kb on + strand, within Psyr_0823 at 931.327 kb on - strand, within Psyr_0823 at 931.577 kb on + strand, within Psyr_0823
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 928,682 + Psyr_0821 0.66 +2.1 928,683 - Psyr_0821 0.66 +2.1 928,683 - Psyr_0821 0.66 -1.1 929,320 + Psyr_0821 0.88 +2.9 929,320 + Psyr_0821 0.88 +1.7 929,320 + Psyr_0821 0.88 +2.1 929,320 + Psyr_0821 0.88 +3.7 929,321 - Psyr_0821 0.88 +3.1 929,321 - Psyr_0821 0.88 +4.2 929,321 - Psyr_0821 0.88 +0.6 929,321 - Psyr_0821 0.88 +4.1 929,321 - Psyr_0821 0.88 +0.0 929,321 - Psyr_0821 0.88 +2.7 929,321 - Psyr_0821 0.88 -2.6 929,321 - Psyr_0821 0.88 -2.5 929,650 + -0.9 929,650 + -0.3 929,651 - +3.9 929,782 + Psyr_0822 0.14 -1.2 929,783 - Psyr_0822 0.14 -2.1 930,524 + +2.5 930,571 + +0.2 930,572 - -1.3 930,591 + +0.1 930,591 + +0.1 930,592 - -0.9 930,592 - +2.3 930,592 - -2.3 930,619 + +1.1 930,619 + -0.5 930,619 + +2.1 930,619 + -1.3 930,619 + -3.4 930,619 + +1.1 930,619 + -0.6 930,620 - -0.3 930,620 - +3.6 930,620 - +2.9 930,620 - -1.6 930,655 + +1.1 931,003 + Psyr_0823 0.23 +5.2 931,003 + Psyr_0823 0.23 -2.1 931,004 - Psyr_0823 0.23 +0.2 931,059 + Psyr_0823 0.26 +2.1 931,059 + Psyr_0823 0.26 +0.4 931,197 + Psyr_0823 0.34 +2.7 931,198 - Psyr_0823 0.34 +1.7 931,198 - Psyr_0823 0.34 -2.4 931,326 + Psyr_0823 0.41 +1.7 931,327 - Psyr_0823 0.41 +3.5 931,577 + Psyr_0823 0.56 +3.7
Or see this region's nucleotide sequence