Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0734 and Psyr_0735 are separated by 363 nucleotides Psyr_0735 and Psyr_0736 overlap by 14 nucleotides Psyr_0736 and Psyr_0737 are separated by 213 nucleotides
Psyr_0734: Psyr_0734 - Protein of unknown function DUF159, at 839,680 to 840,123
_0734
Psyr_0735: Psyr_0735 - Peptidase S24, S26A and S26B, at 840,487 to 840,921
_0735
Psyr_0736: Psyr_0736 - umuC protein, at 840,908 to 841,153
_0736
Psyr_0737: Psyr_0737 - putative transmembrane protein, at 841,367 to 841,780
_0737
Position (kb)
840
841
842 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 839.992 kb on - strand, within Psyr_0734 at 839.997 kb on + strand, within Psyr_0734 at 839.998 kb on - strand, within Psyr_0734 at 840.012 kb on - strand, within Psyr_0734 at 840.012 kb on - strand, within Psyr_0734 at 840.012 kb on - strand, within Psyr_0734 at 840.012 kb on - strand, within Psyr_0734 at 840.012 kb on - strand, within Psyr_0734 at 840.252 kb on + strand at 840.252 kb on + strand at 840.252 kb on + strand at 840.252 kb on + strand at 840.253 kb on - strand at 840.266 kb on + strand at 840.267 kb on - strand at 840.267 kb on - strand at 840.274 kb on + strand at 840.284 kb on - strand at 840.333 kb on - strand at 840.351 kb on - strand at 840.351 kb on - strand at 840.452 kb on + strand at 840.452 kb on + strand at 840.452 kb on + strand at 840.452 kb on + strand at 840.452 kb on + strand at 840.454 kb on + strand at 840.829 kb on - strand, within Psyr_0735 at 840.829 kb on - strand, within Psyr_0735 at 840.829 kb on - strand, within Psyr_0735 at 840.829 kb on - strand, within Psyr_0735 at 840.892 kb on + strand at 840.938 kb on - strand, within Psyr_0736 at 840.957 kb on - strand, within Psyr_0736 at 840.957 kb on - strand, within Psyr_0736 at 840.957 kb on - strand, within Psyr_0736 at 840.957 kb on - strand, within Psyr_0736 at 840.957 kb on - strand, within Psyr_0736 at 840.957 kb on - strand, within Psyr_0736 at 841.143 kb on - strand at 841.143 kb on - strand at 841.152 kb on - strand at 841.152 kb on - strand at 841.152 kb on - strand at 841.154 kb on + strand at 841.154 kb on + strand at 841.154 kb on + strand at 841.154 kb on + strand at 841.154 kb on + strand at 841.155 kb on - strand at 841.159 kb on + strand at 841.160 kb on - strand at 841.160 kb on - strand at 841.223 kb on + strand at 841.223 kb on + strand at 841.224 kb on - strand at 841.340 kb on - strand at 841.354 kb on + strand at 841.357 kb on + strand at 841.446 kb on - strand, within Psyr_0737 at 841.446 kb on - strand, within Psyr_0737 at 841.487 kb on - strand, within Psyr_0737 at 841.487 kb on - strand, within Psyr_0737 at 841.497 kb on + strand, within Psyr_0737 at 841.519 kb on + strand, within Psyr_0737 at 841.520 kb on - strand, within Psyr_0737 at 841.545 kb on - strand, within Psyr_0737 at 841.545 kb on - strand, within Psyr_0737 at 841.571 kb on - strand, within Psyr_0737 at 841.571 kb on - strand, within Psyr_0737 at 841.571 kb on - strand, within Psyr_0737 at 841.571 kb on - strand, within Psyr_0737 at 841.574 kb on + strand, within Psyr_0737 at 841.574 kb on + strand, within Psyr_0737 at 841.575 kb on - strand, within Psyr_0737 at 841.575 kb on - strand, within Psyr_0737 at 841.640 kb on - strand, within Psyr_0737 at 841.641 kb on + strand, within Psyr_0737 at 841.641 kb on + strand, within Psyr_0737 at 841.641 kb on + strand, within Psyr_0737 at 841.642 kb on - strand, within Psyr_0737 at 841.716 kb on + strand, within Psyr_0737 at 841.784 kb on - strand at 841.880 kb on + strand at 841.881 kb on - strand at 841.885 kb on - strand at 841.895 kb on - strand at 841.965 kb on - strand at 842.014 kb on + strand at 842.024 kb on + strand at 842.024 kb on + strand at 842.144 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 839,992 - Psyr_0734 0.70 +3.6 839,997 + Psyr_0734 0.71 +2.1 839,998 - Psyr_0734 0.72 +1.1 840,012 - Psyr_0734 0.75 +0.1 840,012 - Psyr_0734 0.75 +3.7 840,012 - Psyr_0734 0.75 +4.2 840,012 - Psyr_0734 0.75 -0.0 840,012 - Psyr_0734 0.75 -1.6 840,252 + -0.9 840,252 + -1.3 840,252 + -1.5 840,252 + -2.0 840,253 - -2.7 840,266 + +0.1 840,267 - +3.1 840,267 - -1.1 840,274 + -2.1 840,284 - +0.9 840,333 - +4.0 840,351 - +2.7 840,351 - +0.9 840,452 + -0.2 840,452 + -2.7 840,452 + -1.8 840,452 + +3.3 840,452 + +1.0 840,454 + +0.7 840,829 - Psyr_0735 0.79 -2.5 840,829 - Psyr_0735 0.79 +3.1 840,829 - Psyr_0735 0.79 -1.1 840,829 - Psyr_0735 0.79 +4.2 840,892 + +2.9 840,938 - Psyr_0736 0.12 -1.0 840,957 - Psyr_0736 0.20 +2.1 840,957 - Psyr_0736 0.20 -2.4 840,957 - Psyr_0736 0.20 +2.1 840,957 - Psyr_0736 0.20 -3.3 840,957 - Psyr_0736 0.20 +4.0 840,957 - Psyr_0736 0.20 -2.1 841,143 - -0.9 841,143 - -1.4 841,152 - +2.1 841,152 - +0.0 841,152 - +0.5 841,154 + +0.3 841,154 + +1.1 841,154 + +3.3 841,154 + -1.2 841,154 + -1.2 841,155 - -0.9 841,159 + +0.3 841,160 - +0.2 841,160 - +0.7 841,223 + -1.5 841,223 + +2.7 841,224 - -1.9 841,340 - -3.5 841,354 + -1.8 841,357 + -0.8 841,446 - Psyr_0737 0.19 +0.4 841,446 - Psyr_0737 0.19 +1.1 841,487 - Psyr_0737 0.29 -1.6 841,487 - Psyr_0737 0.29 -0.0 841,497 + Psyr_0737 0.31 -0.5 841,519 + Psyr_0737 0.37 +0.1 841,520 - Psyr_0737 0.37 -0.1 841,545 - Psyr_0737 0.43 -0.8 841,545 - Psyr_0737 0.43 +2.1 841,571 - Psyr_0737 0.49 -2.3 841,571 - Psyr_0737 0.49 -2.2 841,571 - Psyr_0737 0.49 +0.1 841,571 - Psyr_0737 0.49 -1.2 841,574 + Psyr_0737 0.50 +0.1 841,574 + Psyr_0737 0.50 +2.7 841,575 - Psyr_0737 0.50 -0.1 841,575 - Psyr_0737 0.50 -0.4 841,640 - Psyr_0737 0.66 -0.3 841,641 + Psyr_0737 0.66 +2.5 841,641 + Psyr_0737 0.66 -0.6 841,641 + Psyr_0737 0.66 +2.5 841,642 - Psyr_0737 0.66 -0.4 841,716 + Psyr_0737 0.84 -1.3 841,784 - +0.1 841,880 + +2.7 841,881 - +3.1 841,885 - +3.1 841,895 - +3.1 841,965 - +2.1 842,014 + -0.3 842,024 + +2.5 842,024 + -0.3 842,144 - -1.1
Or see this region's nucleotide sequence