Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0726 and Psyr_0727 overlap by 4 nucleotides Psyr_0727 and Psyr_0728 are separated by 171 nucleotides Psyr_0728 and Psyr_RNA9 are separated by 294 nucleotides Psyr_RNA9 and Psyr_RNA10 are separated by 29 nucleotides Psyr_RNA10 and Psyr_RNA11 are separated by 5 nucleotides Psyr_RNA11 and Psyr_RNA12 are separated by 95 nucleotides
Psyr_0726: Psyr_0726 - ribosomal large subunit pseudouridine synthase D, at 830,467 to 831,429
_0726
Psyr_0727: Psyr_0727 - Protein of unknown function DUF152, at 831,426 to 832,154
_0727
Psyr_0728: Psyr_0728 - AAA ATPase, central region:Clp, N terminal:Clp, N terminal, at 832,326 to 834,890
_0728
Psyr_RNA9: Psyr_RNA9 - tRNA-Asn, at 835,185 to 835,260
_RNA9
Psyr_RNA10: Psyr_RNA10 - tRNA-Pro, at 835,290 to 835,366
_RNA10
Psyr_RNA11: Psyr_RNA11 - tRNA-Lys, at 835,372 to 835,447
_RNA11
Psyr_RNA12: Psyr_RNA12 - tRNA-Pro, at 835,543 to 835,619
_RNA12
Position (kb) 832
833
834
835 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 831.442 kb on + strand at 831.442 kb on + strand at 831.442 kb on + strand at 831.442 kb on + strand at 831.442 kb on + strand at 831.638 kb on - strand, within Psyr_0727 at 831.638 kb on - strand, within Psyr_0727 at 831.646 kb on + strand, within Psyr_0727 at 831.646 kb on + strand, within Psyr_0727 at 831.646 kb on + strand, within Psyr_0727 at 831.647 kb on - strand, within Psyr_0727 at 831.647 kb on - strand, within Psyr_0727 at 831.718 kb on + strand, within Psyr_0727 at 831.719 kb on - strand, within Psyr_0727 at 831.719 kb on - strand, within Psyr_0727 at 831.719 kb on - strand, within Psyr_0727 at 831.719 kb on - strand, within Psyr_0727 at 832.002 kb on + strand, within Psyr_0727 at 832.002 kb on + strand, within Psyr_0727 at 832.002 kb on + strand, within Psyr_0727 at 832.002 kb on + strand, within Psyr_0727 at 832.002 kb on + strand, within Psyr_0727 at 832.003 kb on - strand, within Psyr_0727 at 832.003 kb on - strand, within Psyr_0727 at 832.003 kb on - strand, within Psyr_0727 at 832.003 kb on - strand, within Psyr_0727 at 832.003 kb on - strand, within Psyr_0727 at 832.037 kb on + strand, within Psyr_0727 at 832.038 kb on - strand, within Psyr_0727 at 832.038 kb on - strand, within Psyr_0727 at 832.038 kb on - strand, within Psyr_0727 at 832.038 kb on - strand, within Psyr_0727 at 832.182 kb on + strand at 832.182 kb on + strand at 832.183 kb on - strand at 832.212 kb on - strand at 832.212 kb on - strand at 832.254 kb on + strand at 832.254 kb on + strand at 832.254 kb on + strand at 832.254 kb on + strand at 832.254 kb on + strand at 832.255 kb on - strand at 832.255 kb on - strand at 832.255 kb on - strand at 832.282 kb on + strand at 832.282 kb on + strand at 832.282 kb on + strand at 832.282 kb on + strand at 832.282 kb on + strand at 832.283 kb on - strand at 832.283 kb on - strand at 832.283 kb on - strand at 832.283 kb on - strand at 832.283 kb on - strand at 832.283 kb on - strand at 832.283 kb on - strand at 832.310 kb on + strand at 832.350 kb on - strand at 832.354 kb on + strand at 832.354 kb on + strand at 832.354 kb on + strand at 832.355 kb on - strand at 832.355 kb on - strand at 832.360 kb on + strand at 832.361 kb on - strand at 832.361 kb on - strand at 832.483 kb on + strand at 832.483 kb on + strand at 832.483 kb on + strand at 832.483 kb on + strand at 832.484 kb on - strand at 832.484 kb on - strand at 832.815 kb on + strand, within Psyr_0728 at 832.815 kb on + strand, within Psyr_0728 at 832.815 kb on + strand, within Psyr_0728 at 832.815 kb on + strand, within Psyr_0728 at 832.815 kb on + strand, within Psyr_0728 at 832.815 kb on + strand, within Psyr_0728 at 832.815 kb on + strand, within Psyr_0728 at 832.815 kb on + strand, within Psyr_0728 at 832.816 kb on - strand, within Psyr_0728 at 832.816 kb on - strand, within Psyr_0728 at 832.816 kb on - strand, within Psyr_0728 at 832.816 kb on - strand, within Psyr_0728 at 833.076 kb on + strand, within Psyr_0728 at 833.077 kb on - strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.289 kb on + strand, within Psyr_0728 at 833.290 kb on - strand, within Psyr_0728 at 833.290 kb on - strand, within Psyr_0728 at 833.290 kb on - strand, within Psyr_0728 at 833.290 kb on - strand, within Psyr_0728 at 833.290 kb on - strand, within Psyr_0728 at 833.290 kb on - strand, within Psyr_0728 at 833.290 kb on - strand, within Psyr_0728 at 833.290 kb on - strand, within Psyr_0728 at 833.290 kb on - strand, within Psyr_0728 at 833.290 kb on - strand, within Psyr_0728 at 833.290 kb on - strand, within Psyr_0728 at 833.290 kb on - strand, within Psyr_0728 at 833.291 kb on + strand, within Psyr_0728 at 833.291 kb on + strand, within Psyr_0728 at 833.291 kb on + strand, within Psyr_0728 at 833.291 kb on + strand, within Psyr_0728 at 833.291 kb on + strand, within Psyr_0728 at 833.291 kb on + strand, within Psyr_0728 at 833.291 kb on + strand, within Psyr_0728 at 833.291 kb on + strand, within Psyr_0728 at 833.291 kb on + strand, within Psyr_0728 at 833.291 kb on + strand, within Psyr_0728 at 833.291 kb on + strand, within Psyr_0728 at 833.291 kb on + strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.292 kb on - strand, within Psyr_0728 at 833.293 kb on + strand, within Psyr_0728 at 833.293 kb on + strand, within Psyr_0728 at 833.293 kb on + strand, within Psyr_0728 at 833.293 kb on + strand, within Psyr_0728 at 833.293 kb on + strand, within Psyr_0728 at 833.293 kb on + strand, within Psyr_0728 at 833.293 kb on + strand, within Psyr_0728 at 833.293 kb on + strand, within Psyr_0728 at 833.294 kb on - strand, within Psyr_0728 at 833.294 kb on - strand, within Psyr_0728 at 833.294 kb on - strand, within Psyr_0728 at 833.294 kb on - strand, within Psyr_0728 at 833.294 kb on - strand, within Psyr_0728 at 833.294 kb on - strand, within Psyr_0728 at 833.294 kb on - strand, within Psyr_0728 at 833.294 kb on - strand, within Psyr_0728 at 833.294 kb on - strand, within Psyr_0728 at 833.294 kb on - strand, within Psyr_0728 at 833.463 kb on + strand, within Psyr_0728 at 833.463 kb on + strand, within Psyr_0728 at 833.463 kb on + strand, within Psyr_0728 at 833.463 kb on + strand, within Psyr_0728 at 833.463 kb on + strand, within Psyr_0728 at 833.464 kb on - strand, within Psyr_0728 at 833.464 kb on - strand, within Psyr_0728 at 833.464 kb on - strand, within Psyr_0728 at 833.464 kb on - strand, within Psyr_0728 at 833.464 kb on - strand, within Psyr_0728 at 833.464 kb on - strand, within Psyr_0728 at 833.465 kb on + strand, within Psyr_0728 at 833.466 kb on - strand, within Psyr_0728 at 833.525 kb on + strand, within Psyr_0728 at 833.535 kb on + strand, within Psyr_0728 at 833.596 kb on + strand, within Psyr_0728 at 833.596 kb on + strand, within Psyr_0728 at 834.282 kb on + strand, within Psyr_0728 at 834.282 kb on + strand, within Psyr_0728 at 834.283 kb on - strand, within Psyr_0728 at 834.336 kb on + strand, within Psyr_0728 at 834.337 kb on - strand, within Psyr_0728 at 834.440 kb on + strand, within Psyr_0728 at 834.440 kb on + strand, within Psyr_0728 at 834.638 kb on + strand at 834.754 kb on - strand at 834.910 kb on - strand at 834.911 kb on + strand at 834.911 kb on + strand at 834.911 kb on + strand at 834.911 kb on + strand at 834.911 kb on + strand at 834.911 kb on + strand at 834.911 kb on + strand at 834.911 kb on + strand at 834.911 kb on + strand at 834.911 kb on + strand at 834.911 kb on + strand at 834.912 kb on - strand at 834.912 kb on - strand at 834.912 kb on - strand at 834.912 kb on - strand at 834.912 kb on - strand at 834.912 kb on - strand at 834.912 kb on - strand at 834.959 kb on + strand at 834.959 kb on + strand at 834.959 kb on + strand at 834.959 kb on + strand at 834.959 kb on + strand at 835.021 kb on + strand at 835.085 kb on + strand at 835.085 kb on + strand at 835.128 kb on + strand at 835.160 kb on + strand at 835.367 kb on + strand at 835.367 kb on + strand at 835.367 kb on + strand at 835.367 kb on + strand at 835.367 kb on + strand at 835.421 kb on + strand, within Psyr_RNA11 at 835.736 kb on + strand at 835.736 kb on + strand at 835.859 kb on + strand at 835.872 kb on + strand at 835.872 kb on + strand at 835.872 kb on + strand at 835.876 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 831,442 + -0.2 831,442 + +0.5 831,442 + -1.6 831,442 + -1.9 831,442 + +3.3 831,638 - Psyr_0727 0.29 +3.1 831,638 - Psyr_0727 0.29 +1.4 831,646 + Psyr_0727 0.30 +1.3 831,646 + Psyr_0727 0.30 -0.9 831,646 + Psyr_0727 0.30 -1.1 831,647 - Psyr_0727 0.30 +2.9 831,647 - Psyr_0727 0.30 -0.9 831,718 + Psyr_0727 0.40 +0.6 831,719 - Psyr_0727 0.40 +2.0 831,719 - Psyr_0727 0.40 +0.2 831,719 - Psyr_0727 0.40 -1.0 831,719 - Psyr_0727 0.40 -0.8 832,002 + Psyr_0727 0.79 -0.9 832,002 + Psyr_0727 0.79 +1.7 832,002 + Psyr_0727 0.79 +2.1 832,002 + Psyr_0727 0.79 +1.7 832,002 + Psyr_0727 0.79 +1.7 832,003 - Psyr_0727 0.79 +0.6 832,003 - Psyr_0727 0.79 -0.2 832,003 - Psyr_0727 0.79 -0.1 832,003 - Psyr_0727 0.79 +4.0 832,003 - Psyr_0727 0.79 +2.5 832,037 + Psyr_0727 0.84 -3.1 832,038 - Psyr_0727 0.84 -1.4 832,038 - Psyr_0727 0.84 +2.7 832,038 - Psyr_0727 0.84 +3.1 832,038 - Psyr_0727 0.84 -1.4 832,182 + -0.7 832,182 + -3.3 832,183 - -1.3 832,212 - +3.5 832,212 - -3.5 832,254 + +0.9 832,254 + -1.0 832,254 + +1.2 832,254 + -0.5 832,254 + +0.1 832,255 - -0.8 832,255 - -2.3 832,255 - +2.1 832,282 + -0.9 832,282 + -4.4 832,282 + -1.2 832,282 + -0.3 832,282 + -1.6 832,283 - +1.1 832,283 - -1.3 832,283 - +1.5 832,283 - -0.1 832,283 - -1.5 832,283 - +2.1 832,283 - -1.8 832,310 + -0.7 832,350 - +3.8 832,354 + -0.7 832,354 + -0.9 832,354 + -0.7 832,355 - +4.5 832,355 - +3.1 832,360 + -1.0 832,361 - -1.2 832,361 - +0.1 832,483 + +3.9 832,483 + -0.1 832,483 + -3.9 832,483 + -0.2 832,484 - -3.4 832,484 - +3.6 832,815 + Psyr_0728 0.19 -1.2 832,815 + Psyr_0728 0.19 +5.3 832,815 + Psyr_0728 0.19 -0.4 832,815 + Psyr_0728 0.19 -1.6 832,815 + Psyr_0728 0.19 -2.1 832,815 + Psyr_0728 0.19 -1.2 832,815 + Psyr_0728 0.19 +3.7 832,815 + Psyr_0728 0.19 -3.0 832,816 - Psyr_0728 0.19 +4.1 832,816 - Psyr_0728 0.19 +3.5 832,816 - Psyr_0728 0.19 -1.6 832,816 - Psyr_0728 0.19 +3.7 833,076 + Psyr_0728 0.29 +2.5 833,077 - Psyr_0728 0.29 +3.5 833,289 + Psyr_0728 0.38 +0.7 833,289 + Psyr_0728 0.38 +3.8 833,289 + Psyr_0728 0.38 -0.6 833,289 + Psyr_0728 0.38 -0.8 833,289 + Psyr_0728 0.38 -2.0 833,289 + Psyr_0728 0.38 -0.2 833,289 + Psyr_0728 0.38 +3.8 833,289 + Psyr_0728 0.38 +0.8 833,289 + Psyr_0728 0.38 -0.8 833,289 + Psyr_0728 0.38 +3.7 833,289 + Psyr_0728 0.38 +1.3 833,289 + Psyr_0728 0.38 +1.6 833,289 + Psyr_0728 0.38 -1.6 833,289 + Psyr_0728 0.38 +3.3 833,289 + Psyr_0728 0.38 +2.7 833,290 - Psyr_0728 0.38 +0.9 833,290 - Psyr_0728 0.38 +3.6 833,290 - Psyr_0728 0.38 +1.7 833,290 - Psyr_0728 0.38 +4.5 833,290 - Psyr_0728 0.38 +1.7 833,290 - Psyr_0728 0.38 +2.5 833,290 - Psyr_0728 0.38 +3.6 833,290 - Psyr_0728 0.38 -0.4 833,290 - Psyr_0728 0.38 +0.9 833,290 - Psyr_0728 0.38 +0.6 833,290 - Psyr_0728 0.38 +1.9 833,290 - Psyr_0728 0.38 -1.5 833,291 + Psyr_0728 0.38 -1.9 833,291 + Psyr_0728 0.38 +3.3 833,291 + Psyr_0728 0.38 +0.8 833,291 + Psyr_0728 0.38 +3.3 833,291 + Psyr_0728 0.38 +0.1 833,291 + Psyr_0728 0.38 +0.3 833,291 + Psyr_0728 0.38 -2.1 833,291 + Psyr_0728 0.38 -1.6 833,291 + Psyr_0728 0.38 +3.6 833,291 + Psyr_0728 0.38 -0.7 833,291 + Psyr_0728 0.38 +0.3 833,291 + Psyr_0728 0.38 -1.7 833,292 - Psyr_0728 0.38 +0.0 833,292 - Psyr_0728 0.38 -1.9 833,292 - Psyr_0728 0.38 +1.3 833,292 - Psyr_0728 0.38 -0.5 833,292 - Psyr_0728 0.38 -0.3 833,292 - Psyr_0728 0.38 -1.6 833,292 - Psyr_0728 0.38 +3.6 833,292 - Psyr_0728 0.38 +0.4 833,292 - Psyr_0728 0.38 -0.9 833,292 - Psyr_0728 0.38 -0.5 833,292 - Psyr_0728 0.38 -0.2 833,292 - Psyr_0728 0.38 +3.3 833,292 - Psyr_0728 0.38 +1.1 833,292 - Psyr_0728 0.38 +3.9 833,292 - Psyr_0728 0.38 -2.1 833,292 - Psyr_0728 0.38 +1.1 833,292 - Psyr_0728 0.38 +0.1 833,292 - Psyr_0728 0.38 -2.7 833,292 - Psyr_0728 0.38 +2.0 833,292 - Psyr_0728 0.38 -1.1 833,293 + Psyr_0728 0.38 +0.1 833,293 + Psyr_0728 0.38 +2.1 833,293 + Psyr_0728 0.38 -0.6 833,293 + Psyr_0728 0.38 -0.4 833,293 + Psyr_0728 0.38 -2.3 833,293 + Psyr_0728 0.38 +3.7 833,293 + Psyr_0728 0.38 -0.9 833,293 + Psyr_0728 0.38 -0.2 833,294 - Psyr_0728 0.38 -0.3 833,294 - Psyr_0728 0.38 -3.8 833,294 - Psyr_0728 0.38 -1.6 833,294 - Psyr_0728 0.38 -2.7 833,294 - Psyr_0728 0.38 -0.7 833,294 - Psyr_0728 0.38 +0.5 833,294 - Psyr_0728 0.38 -0.8 833,294 - Psyr_0728 0.38 -1.2 833,294 - Psyr_0728 0.38 -2.7 833,294 - Psyr_0728 0.38 -0.6 833,463 + Psyr_0728 0.44 +0.1 833,463 + Psyr_0728 0.44 -2.9 833,463 + Psyr_0728 0.44 +3.8 833,463 + Psyr_0728 0.44 +3.6 833,463 + Psyr_0728 0.44 +3.3 833,464 - Psyr_0728 0.44 +0.7 833,464 - Psyr_0728 0.44 -1.9 833,464 - Psyr_0728 0.44 -0.6 833,464 - Psyr_0728 0.44 -2.7 833,464 - Psyr_0728 0.44 +1.0 833,464 - Psyr_0728 0.44 -0.2 833,465 + Psyr_0728 0.44 +3.5 833,466 - Psyr_0728 0.44 +1.1 833,525 + Psyr_0728 0.47 +0.7 833,535 + Psyr_0728 0.47 +0.3 833,596 + Psyr_0728 0.50 +1.1 833,596 + Psyr_0728 0.50 -1.4 834,282 + Psyr_0728 0.76 +0.9 834,282 + Psyr_0728 0.76 +1.1 834,283 - Psyr_0728 0.76 +0.3 834,336 + Psyr_0728 0.78 +0.8 834,337 - Psyr_0728 0.78 +0.0 834,440 + Psyr_0728 0.82 +1.7 834,440 + Psyr_0728 0.82 -2.2 834,638 + -3.6 834,754 - +3.6 834,910 - +1.7 834,911 + +4.5 834,911 + -0.8 834,911 + +3.6 834,911 + -0.8 834,911 + +3.8 834,911 + -1.4 834,911 + +1.0 834,911 + +0.8 834,911 + +1.7 834,911 + +0.9 834,911 + -3.1 834,912 - +1.1 834,912 - -0.1 834,912 - +1.1 834,912 - -1.4 834,912 - -2.3 834,912 - +2.5 834,912 - -0.6 834,959 + -0.6 834,959 + -0.5 834,959 + +0.5 834,959 + -3.0 834,959 + -1.1 835,021 + -2.3 835,085 + -1.2 835,085 + -2.9 835,128 + +0.1 835,160 + +0.1 835,367 + +1.5 835,367 + +0.1 835,367 + +0.6 835,367 + +0.1 835,367 + -0.6 835,421 + Psyr_RNA11 0.64 +0.1 835,736 + -0.7 835,736 + +1.3 835,859 + +1.1 835,872 + -1.1 835,872 + -0.4 835,872 + -0.7 835,876 + +3.3
Or see this region's nucleotide sequence