Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0721 and Psyr_0722 are separated by 112 nucleotides Psyr_0722 and Psyr_0723 are separated by 2 nucleotides
Psyr_0721: Psyr_0721 - glycine oxidase, at 824,797 to 825,900
_0721
Psyr_0722: Psyr_0722 - Two-component response regulator PilR, at 826,013 to 827,350
_0722
Psyr_0723: Psyr_0723 - Sensor protein PilS, at 827,353 to 828,945
_0723
Position (kb) 826
827
828 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 825.157 kb on - strand, within Psyr_0721 at 825.200 kb on - strand, within Psyr_0721 at 825.214 kb on + strand, within Psyr_0721 at 825.214 kb on + strand, within Psyr_0721 at 825.214 kb on + strand, within Psyr_0721 at 825.214 kb on + strand, within Psyr_0721 at 825.214 kb on + strand, within Psyr_0721 at 825.214 kb on + strand, within Psyr_0721 at 825.214 kb on + strand, within Psyr_0721 at 825.215 kb on - strand, within Psyr_0721 at 825.215 kb on - strand, within Psyr_0721 at 825.215 kb on - strand, within Psyr_0721 at 825.215 kb on - strand, within Psyr_0721 at 825.215 kb on - strand, within Psyr_0721 at 825.215 kb on - strand, within Psyr_0721 at 825.236 kb on + strand, within Psyr_0721 at 825.236 kb on + strand, within Psyr_0721 at 825.236 kb on + strand, within Psyr_0721 at 825.236 kb on + strand, within Psyr_0721 at 825.237 kb on - strand, within Psyr_0721 at 825.237 kb on - strand, within Psyr_0721 at 825.237 kb on - strand, within Psyr_0721 at 825.237 kb on - strand, within Psyr_0721 at 825.484 kb on + strand, within Psyr_0721 at 825.484 kb on + strand, within Psyr_0721 at 825.485 kb on - strand, within Psyr_0721 at 825.898 kb on + strand at 825.898 kb on + strand at 825.898 kb on + strand at 825.898 kb on + strand at 825.899 kb on - strand at 825.938 kb on + strand at 825.939 kb on - strand at 825.939 kb on - strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.991 kb on + strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 825.992 kb on - strand at 826.005 kb on + strand at 826.149 kb on + strand, within Psyr_0722 at 826.149 kb on + strand, within Psyr_0722 at 826.149 kb on + strand, within Psyr_0722 at 826.264 kb on - strand, within Psyr_0722 at 826.315 kb on - strand, within Psyr_0722 at 826.473 kb on + strand, within Psyr_0722 at 826.474 kb on - strand, within Psyr_0722 at 826.474 kb on - strand, within Psyr_0722 at 826.474 kb on - strand, within Psyr_0722 at 826.474 kb on - strand, within Psyr_0722 at 826.474 kb on - strand, within Psyr_0722 at 826.474 kb on - strand, within Psyr_0722 at 826.835 kb on + strand, within Psyr_0722 at 826.872 kb on + strand, within Psyr_0722 at 826.872 kb on + strand, within Psyr_0722 at 826.872 kb on + strand, within Psyr_0722 at 826.872 kb on + strand, within Psyr_0722 at 826.873 kb on - strand, within Psyr_0722 at 826.873 kb on - strand, within Psyr_0722 at 826.873 kb on - strand, within Psyr_0722 at 826.873 kb on - strand, within Psyr_0722 at 826.921 kb on + strand, within Psyr_0722 at 826.921 kb on + strand, within Psyr_0722 at 826.922 kb on - strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.141 kb on + strand, within Psyr_0722 at 827.142 kb on - strand, within Psyr_0722 at 827.142 kb on - strand, within Psyr_0722 at 827.142 kb on - strand, within Psyr_0722 at 827.142 kb on - strand, within Psyr_0722 at 827.142 kb on - strand, within Psyr_0722 at 827.142 kb on - strand, within Psyr_0722 at 827.142 kb on - strand, within Psyr_0722 at 827.142 kb on - strand, within Psyr_0722 at 827.142 kb on - strand, within Psyr_0722 at 827.142 kb on - strand, within Psyr_0722 at 827.324 kb on + strand at 827.382 kb on + strand at 827.382 kb on + strand at 827.383 kb on - strand at 827.383 kb on - strand at 827.383 kb on - strand at 827.383 kb on - strand at 827.421 kb on - strand at 827.465 kb on + strand at 827.465 kb on + strand at 827.465 kb on + strand at 827.465 kb on + strand at 827.465 kb on + strand at 827.465 kb on + strand at 827.466 kb on - strand at 827.466 kb on - strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.467 kb on + strand at 827.468 kb on - strand at 827.468 kb on - strand at 827.468 kb on - strand at 827.468 kb on - strand at 827.468 kb on - strand at 827.468 kb on - strand at 827.468 kb on - strand at 827.468 kb on - strand at 827.599 kb on + strand, within Psyr_0723 at 827.600 kb on - strand, within Psyr_0723 at 827.600 kb on - strand, within Psyr_0723 at 827.642 kb on + strand, within Psyr_0723 at 827.642 kb on + strand, within Psyr_0723 at 827.865 kb on + strand, within Psyr_0723 at 827.941 kb on - strand, within Psyr_0723 at 827.983 kb on + strand, within Psyr_0723 at 827.984 kb on - strand, within Psyr_0723 at 827.984 kb on - strand, within Psyr_0723 at 827.984 kb on - strand, within Psyr_0723 at 828.268 kb on + strand, within Psyr_0723 at 828.268 kb on + strand, within Psyr_0723 at 828.268 kb on + strand, within Psyr_0723 at 828.269 kb on - strand, within Psyr_0723 at 828.269 kb on - strand, within Psyr_0723 at 828.269 kb on - strand, within Psyr_0723 at 828.269 kb on - strand, within Psyr_0723
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 825,157 - Psyr_0721 0.33 +2.1 825,200 - Psyr_0721 0.37 +3.1 825,214 + Psyr_0721 0.38 +3.7 825,214 + Psyr_0721 0.38 +3.5 825,214 + Psyr_0721 0.38 +2.7 825,214 + Psyr_0721 0.38 -1.7 825,214 + Psyr_0721 0.38 -0.3 825,214 + Psyr_0721 0.38 -0.1 825,214 + Psyr_0721 0.38 -2.1 825,215 - Psyr_0721 0.38 -4.0 825,215 - Psyr_0721 0.38 -0.6 825,215 - Psyr_0721 0.38 +1.7 825,215 - Psyr_0721 0.38 -1.6 825,215 - Psyr_0721 0.38 -1.4 825,215 - Psyr_0721 0.38 -1.7 825,236 + Psyr_0721 0.40 +4.3 825,236 + Psyr_0721 0.40 -3.1 825,236 + Psyr_0721 0.40 -1.5 825,236 + Psyr_0721 0.40 -1.5 825,237 - Psyr_0721 0.40 -2.3 825,237 - Psyr_0721 0.40 +1.7 825,237 - Psyr_0721 0.40 +1.7 825,237 - Psyr_0721 0.40 +1.0 825,484 + Psyr_0721 0.62 +2.9 825,484 + Psyr_0721 0.62 -0.9 825,485 - Psyr_0721 0.62 -0.9 825,898 + +4.6 825,898 + -1.3 825,898 + +2.1 825,898 + +2.1 825,899 - -0.4 825,938 + -0.5 825,939 - -0.3 825,939 - -1.6 825,991 + +0.1 825,991 + -0.2 825,991 + -3.4 825,991 + +0.1 825,991 + -1.0 825,991 + -0.0 825,991 + +1.7 825,991 + -2.7 825,991 + +0.4 825,991 + +2.7 825,991 + +2.7 825,991 + +3.3 825,991 + -1.9 825,991 + +0.3 825,991 + -1.3 825,991 + +2.9 825,991 + -2.3 825,991 + +0.4 825,991 + +2.7 825,991 + +2.7 825,991 + +4.7 825,991 + +1.1 825,991 + +0.9 825,992 - +3.6 825,992 - +0.5 825,992 - +2.6 825,992 - +0.5 825,992 - +3.8 825,992 - +2.1 825,992 - +4.3 825,992 - -0.1 825,992 - -0.7 825,992 - +2.7 825,992 - +0.1 825,992 - -2.7 825,992 - +2.7 825,992 - -3.0 825,992 - +3.6 825,992 - -1.4 825,992 - +3.7 825,992 - +4.4 825,992 - +0.7 825,992 - +2.1 825,992 - +1.7 826,005 + +4.2 826,149 + Psyr_0722 0.10 -1.4 826,149 + Psyr_0722 0.10 +0.1 826,149 + Psyr_0722 0.10 -1.9 826,264 - Psyr_0722 0.19 -0.7 826,315 - Psyr_0722 0.23 -0.6 826,473 + Psyr_0722 0.34 -0.9 826,474 - Psyr_0722 0.34 +1.5 826,474 - Psyr_0722 0.34 +2.5 826,474 - Psyr_0722 0.34 +1.5 826,474 - Psyr_0722 0.34 +0.8 826,474 - Psyr_0722 0.34 -1.3 826,474 - Psyr_0722 0.34 -1.7 826,835 + Psyr_0722 0.61 -1.1 826,872 + Psyr_0722 0.64 -2.2 826,872 + Psyr_0722 0.64 -2.9 826,872 + Psyr_0722 0.64 -0.5 826,872 + Psyr_0722 0.64 -1.2 826,873 - Psyr_0722 0.64 -0.8 826,873 - Psyr_0722 0.64 -1.5 826,873 - Psyr_0722 0.64 -1.1 826,873 - Psyr_0722 0.64 +1.1 826,921 + Psyr_0722 0.68 -0.7 826,921 + Psyr_0722 0.68 +2.7 826,922 - Psyr_0722 0.68 +1.7 827,141 + Psyr_0722 0.84 -2.7 827,141 + Psyr_0722 0.84 +0.1 827,141 + Psyr_0722 0.84 -0.9 827,141 + Psyr_0722 0.84 -1.7 827,141 + Psyr_0722 0.84 +0.6 827,141 + Psyr_0722 0.84 +1.1 827,141 + Psyr_0722 0.84 -0.3 827,141 + Psyr_0722 0.84 +2.5 827,141 + Psyr_0722 0.84 -0.4 827,141 + Psyr_0722 0.84 -2.3 827,141 + Psyr_0722 0.84 -1.7 827,141 + Psyr_0722 0.84 -1.6 827,141 + Psyr_0722 0.84 -2.1 827,141 + Psyr_0722 0.84 -1.4 827,142 - Psyr_0722 0.84 -2.7 827,142 - Psyr_0722 0.84 -0.9 827,142 - Psyr_0722 0.84 +1.9 827,142 - Psyr_0722 0.84 +0.2 827,142 - Psyr_0722 0.84 -1.4 827,142 - Psyr_0722 0.84 -0.6 827,142 - Psyr_0722 0.84 +2.5 827,142 - Psyr_0722 0.84 -1.1 827,142 - Psyr_0722 0.84 +3.9 827,142 - Psyr_0722 0.84 -1.3 827,324 + +0.1 827,382 + +2.1 827,382 + -1.0 827,383 - -0.6 827,383 - -0.5 827,383 - -0.9 827,383 - +0.4 827,421 - +0.5 827,465 + -2.3 827,465 + +3.5 827,465 + +0.1 827,465 + -1.8 827,465 + -0.8 827,465 + +2.1 827,466 - +0.6 827,466 - +1.3 827,467 + -1.4 827,467 + +0.0 827,467 + +3.9 827,467 + -1.6 827,467 + +0.1 827,467 + -1.0 827,467 + -2.3 827,467 + -1.4 827,467 + +1.7 827,467 + +0.1 827,467 + -0.5 827,467 + +0.5 827,467 + +3.9 827,467 + -1.3 827,467 + +2.7 827,467 + +1.7 827,467 + +2.7 827,468 - +2.5 827,468 - -0.9 827,468 - -0.9 827,468 - -0.4 827,468 - +1.3 827,468 - -1.2 827,468 - -0.4 827,468 - +0.7 827,599 + Psyr_0723 0.15 +0.1 827,600 - Psyr_0723 0.16 -0.0 827,600 - Psyr_0723 0.16 +2.9 827,642 + Psyr_0723 0.18 -0.9 827,642 + Psyr_0723 0.18 +1.0 827,865 + Psyr_0723 0.32 -0.9 827,941 - Psyr_0723 0.37 -0.9 827,983 + Psyr_0723 0.40 +1.1 827,984 - Psyr_0723 0.40 -0.7 827,984 - Psyr_0723 0.40 +3.9 827,984 - Psyr_0723 0.40 +3.7 828,268 + Psyr_0723 0.57 +4.2 828,268 + Psyr_0723 0.57 -1.0 828,268 + Psyr_0723 0.57 +0.6 828,269 - Psyr_0723 0.58 +3.3 828,269 - Psyr_0723 0.58 -0.4 828,269 - Psyr_0723 0.58 -0.4 828,269 - Psyr_0723 0.58 +1.7
Or see this region's nucleotide sequence