Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0644 and Psyr_0645 are separated by 108 nucleotides Psyr_0645 and Psyr_0646 are separated by 185 nucleotides Psyr_0646 and Psyr_0647 are separated by 32 nucleotides
Psyr_0644: Psyr_0644 - Short-chain dehydrogenase/reductase SDR, at 731,340 to 732,695
_0644
Psyr_0645: Psyr_0645 - 3-ketoacyl-CoA thiolase, at 732,804 to 734,081
_0645
Psyr_0646: Psyr_0646 - conserved hypothetical protein, at 734,267 to 734,545
_0646
Psyr_0647: Psyr_0647 - Protein of unknown function DUF726, at 734,578 to 735,975
_0647
Position (kb)
732
733
734
735 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 731.832 kb on + strand, within Psyr_0644 at 731.832 kb on + strand, within Psyr_0644 at 731.832 kb on + strand, within Psyr_0644 at 731.832 kb on + strand, within Psyr_0644 at 731.832 kb on + strand, within Psyr_0644 at 731.832 kb on + strand, within Psyr_0644 at 731.832 kb on + strand, within Psyr_0644 at 731.832 kb on + strand, within Psyr_0644 at 731.833 kb on - strand, within Psyr_0644 at 731.833 kb on - strand, within Psyr_0644 at 731.833 kb on - strand, within Psyr_0644 at 731.833 kb on - strand, within Psyr_0644 at 731.833 kb on - strand, within Psyr_0644 at 731.833 kb on - strand, within Psyr_0644 at 731.892 kb on - strand, within Psyr_0644 at 731.959 kb on - strand, within Psyr_0644 at 731.959 kb on - strand, within Psyr_0644 at 731.959 kb on - strand, within Psyr_0644 at 732.494 kb on - strand, within Psyr_0644 at 732.494 kb on - strand, within Psyr_0644 at 732.494 kb on - strand, within Psyr_0644 at 732.494 kb on - strand, within Psyr_0644 at 732.677 kb on + strand at 732.678 kb on - strand at 732.679 kb on + strand at 732.679 kb on + strand at 732.680 kb on - strand at 732.680 kb on - strand at 732.680 kb on - strand at 732.680 kb on - strand at 733.057 kb on - strand, within Psyr_0645 at 733.057 kb on - strand, within Psyr_0645 at 733.219 kb on + strand, within Psyr_0645 at 733.219 kb on + strand, within Psyr_0645 at 733.370 kb on - strand, within Psyr_0645 at 733.370 kb on - strand, within Psyr_0645 at 733.465 kb on - strand, within Psyr_0645 at 733.465 kb on - strand, within Psyr_0645 at 733.791 kb on + strand, within Psyr_0645 at 733.829 kb on + strand, within Psyr_0645 at 734.104 kb on + strand at 734.104 kb on + strand at 734.104 kb on + strand at 734.105 kb on - strand at 734.120 kb on + strand at 734.121 kb on - strand at 734.121 kb on - strand at 734.121 kb on - strand at 734.148 kb on + strand at 734.160 kb on - strand at 734.240 kb on + strand at 734.241 kb on - strand at 734.261 kb on + strand at 734.261 kb on + strand at 734.262 kb on - strand at 734.262 kb on - strand at 734.278 kb on + strand at 734.292 kb on + strand at 734.321 kb on + strand, within Psyr_0646 at 734.321 kb on + strand, within Psyr_0646 at 734.321 kb on + strand, within Psyr_0646 at 734.321 kb on + strand, within Psyr_0646 at 734.321 kb on + strand, within Psyr_0646 at 734.321 kb on + strand, within Psyr_0646 at 734.322 kb on - strand, within Psyr_0646 at 734.322 kb on - strand, within Psyr_0646 at 734.322 kb on - strand, within Psyr_0646 at 734.322 kb on - strand, within Psyr_0646 at 734.322 kb on - strand, within Psyr_0646 at 734.322 kb on - strand, within Psyr_0646 at 734.322 kb on - strand, within Psyr_0646 at 734.363 kb on + strand, within Psyr_0646 at 734.363 kb on + strand, within Psyr_0646 at 734.363 kb on + strand, within Psyr_0646 at 734.363 kb on + strand, within Psyr_0646 at 734.363 kb on + strand, within Psyr_0646 at 734.363 kb on + strand, within Psyr_0646 at 734.363 kb on + strand, within Psyr_0646 at 734.363 kb on + strand, within Psyr_0646 at 734.363 kb on + strand, within Psyr_0646 at 734.363 kb on + strand, within Psyr_0646 at 734.364 kb on - strand, within Psyr_0646 at 734.364 kb on - strand, within Psyr_0646 at 734.364 kb on - strand, within Psyr_0646 at 734.364 kb on - strand, within Psyr_0646 at 734.364 kb on - strand, within Psyr_0646 at 734.364 kb on - strand, within Psyr_0646 at 734.364 kb on - strand, within Psyr_0646 at 734.364 kb on - strand, within Psyr_0646 at 734.365 kb on + strand, within Psyr_0646 at 734.365 kb on + strand, within Psyr_0646 at 734.366 kb on - strand, within Psyr_0646 at 734.366 kb on - strand, within Psyr_0646 at 734.436 kb on + strand, within Psyr_0646 at 734.436 kb on + strand, within Psyr_0646 at 734.437 kb on - strand, within Psyr_0646 at 734.437 kb on - strand, within Psyr_0646 at 734.444 kb on + strand, within Psyr_0646 at 734.444 kb on + strand, within Psyr_0646 at 734.445 kb on - strand, within Psyr_0646 at 734.445 kb on - strand, within Psyr_0646 at 734.445 kb on - strand, within Psyr_0646 at 734.445 kb on - strand, within Psyr_0646 at 734.445 kb on - strand, within Psyr_0646 at 734.445 kb on - strand, within Psyr_0646 at 734.511 kb on + strand, within Psyr_0646 at 734.698 kb on - strand at 735.050 kb on + strand, within Psyr_0647 at 735.050 kb on + strand, within Psyr_0647 at 735.050 kb on + strand, within Psyr_0647 at 735.050 kb on + strand, within Psyr_0647 at 735.050 kb on + strand, within Psyr_0647 at 735.050 kb on + strand, within Psyr_0647 at 735.051 kb on - strand, within Psyr_0647
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 731,832 + Psyr_0644 0.36 +1.4 731,832 + Psyr_0644 0.36 -1.4 731,832 + Psyr_0644 0.36 +1.1 731,832 + Psyr_0644 0.36 -3.7 731,832 + Psyr_0644 0.36 -0.6 731,832 + Psyr_0644 0.36 +4.1 731,832 + Psyr_0644 0.36 -1.2 731,832 + Psyr_0644 0.36 +2.1 731,833 - Psyr_0644 0.36 +3.6 731,833 - Psyr_0644 0.36 -1.2 731,833 - Psyr_0644 0.36 -1.1 731,833 - Psyr_0644 0.36 -1.9 731,833 - Psyr_0644 0.36 -2.4 731,833 - Psyr_0644 0.36 +2.9 731,892 - Psyr_0644 0.41 -1.6 731,959 - Psyr_0644 0.46 +2.5 731,959 - Psyr_0644 0.46 +1.7 731,959 - Psyr_0644 0.46 -1.4 732,494 - Psyr_0644 0.85 -1.1 732,494 - Psyr_0644 0.85 -1.7 732,494 - Psyr_0644 0.85 -0.8 732,494 - Psyr_0644 0.85 -1.5 732,677 + +4.5 732,678 - +0.7 732,679 + -3.3 732,679 + +1.7 732,680 - +2.7 732,680 - +2.5 732,680 - +1.1 732,680 - -0.9 733,057 - Psyr_0645 0.20 +4.5 733,057 - Psyr_0645 0.20 -0.9 733,219 + Psyr_0645 0.32 +0.2 733,219 + Psyr_0645 0.32 +1.7 733,370 - Psyr_0645 0.44 -0.6 733,370 - Psyr_0645 0.44 +3.9 733,465 - Psyr_0645 0.52 -3.0 733,465 - Psyr_0645 0.52 +0.6 733,791 + Psyr_0645 0.77 +0.4 733,829 + Psyr_0645 0.80 +4.1 734,104 + -0.9 734,104 + -0.9 734,104 + -1.9 734,105 - +4.0 734,120 + -0.7 734,121 - +3.7 734,121 - +1.1 734,121 - -1.4 734,148 + +3.6 734,160 - -0.2 734,240 + +0.0 734,241 - +4.1 734,261 + +0.8 734,261 + -1.2 734,262 - +3.5 734,262 - -1.4 734,278 + +1.7 734,292 + -0.4 734,321 + Psyr_0646 0.19 +1.7 734,321 + Psyr_0646 0.19 +2.1 734,321 + Psyr_0646 0.19 +1.9 734,321 + Psyr_0646 0.19 +2.5 734,321 + Psyr_0646 0.19 -0.7 734,321 + Psyr_0646 0.19 +3.5 734,322 - Psyr_0646 0.20 -2.0 734,322 - Psyr_0646 0.20 +0.6 734,322 - Psyr_0646 0.20 +2.9 734,322 - Psyr_0646 0.20 +0.1 734,322 - Psyr_0646 0.20 +2.7 734,322 - Psyr_0646 0.20 -0.8 734,322 - Psyr_0646 0.20 +4.2 734,363 + Psyr_0646 0.34 +2.7 734,363 + Psyr_0646 0.34 +0.5 734,363 + Psyr_0646 0.34 -0.6 734,363 + Psyr_0646 0.34 -0.6 734,363 + Psyr_0646 0.34 +3.6 734,363 + Psyr_0646 0.34 +3.3 734,363 + Psyr_0646 0.34 -0.6 734,363 + Psyr_0646 0.34 -0.4 734,363 + Psyr_0646 0.34 -2.1 734,363 + Psyr_0646 0.34 -1.8 734,364 - Psyr_0646 0.35 -1.4 734,364 - Psyr_0646 0.35 -1.7 734,364 - Psyr_0646 0.35 +0.2 734,364 - Psyr_0646 0.35 +2.7 734,364 - Psyr_0646 0.35 +1.0 734,364 - Psyr_0646 0.35 -1.0 734,364 - Psyr_0646 0.35 +4.3 734,364 - Psyr_0646 0.35 -0.6 734,365 + Psyr_0646 0.35 +1.1 734,365 + Psyr_0646 0.35 -2.0 734,366 - Psyr_0646 0.35 -2.9 734,366 - Psyr_0646 0.35 -1.1 734,436 + Psyr_0646 0.61 +4.2 734,436 + Psyr_0646 0.61 +0.1 734,437 - Psyr_0646 0.61 +1.1 734,437 - Psyr_0646 0.61 -2.0 734,444 + Psyr_0646 0.63 +2.5 734,444 + Psyr_0646 0.63 -1.6 734,445 - Psyr_0646 0.64 +1.7 734,445 - Psyr_0646 0.64 +0.6 734,445 - Psyr_0646 0.64 +1.1 734,445 - Psyr_0646 0.64 -1.2 734,445 - Psyr_0646 0.64 +3.5 734,445 - Psyr_0646 0.64 -0.5 734,511 + Psyr_0646 0.87 +5.0 734,698 - -0.9 735,050 + Psyr_0647 0.34 +1.1 735,050 + Psyr_0647 0.34 +0.2 735,050 + Psyr_0647 0.34 +1.7 735,050 + Psyr_0647 0.34 -0.4 735,050 + Psyr_0647 0.34 -0.7 735,050 + Psyr_0647 0.34 -0.2 735,051 - Psyr_0647 0.34 -1.5
Or see this region's nucleotide sequence