Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0635 and Psyr_0636 are separated by 203 nucleotides Psyr_0636 and Psyr_0637 overlap by 4 nucleotides Psyr_0637 and Psyr_0638 are separated by 14 nucleotides Psyr_0638 and Psyr_0639 are separated by 84 nucleotides
Psyr_0635: Psyr_0635 - MaoC-like dehydratase, at 725,613 to 726,068
_0635
Psyr_0636: Psyr_0636 - LrgA, at 726,272 to 726,634
_0636
Psyr_0637: Psyr_0637 - LrgB-like protein, at 726,631 to 727,347
_0637
Psyr_0638: Psyr_0638 - Peptidase S16, lon N-terminal, at 727,362 to 727,952
_0638
Psyr_0639: Psyr_0639 - lipoprotein, putative, at 728,037 to 728,603
_0639
Position (kb)
726
727
728 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 725.632 kb on - strand at 725.803 kb on + strand, within Psyr_0635 at 725.803 kb on + strand, within Psyr_0635 at 725.835 kb on + strand, within Psyr_0635 at 725.836 kb on - strand, within Psyr_0635 at 725.836 kb on - strand, within Psyr_0635 at 725.981 kb on + strand, within Psyr_0635 at 726.034 kb on + strand at 726.034 kb on + strand at 726.034 kb on + strand at 726.035 kb on - strand at 726.035 kb on - strand at 726.035 kb on - strand at 726.035 kb on - strand at 726.035 kb on - strand at 726.068 kb on + strand at 726.068 kb on + strand at 726.068 kb on + strand at 726.068 kb on + strand at 726.068 kb on + strand at 726.069 kb on - strand at 726.069 kb on - strand at 726.069 kb on - strand at 726.095 kb on + strand at 726.095 kb on + strand at 726.096 kb on - strand at 726.096 kb on - strand at 726.096 kb on - strand at 726.096 kb on - strand at 726.223 kb on + strand at 726.223 kb on + strand at 726.223 kb on + strand at 726.224 kb on - strand at 726.224 kb on - strand at 726.224 kb on - strand at 726.224 kb on - strand at 726.224 kb on - strand at 726.224 kb on - strand at 726.225 kb on + strand at 726.225 kb on + strand at 726.225 kb on + strand at 726.226 kb on - strand at 726.226 kb on - strand at 726.226 kb on - strand at 726.255 kb on + strand at 726.255 kb on + strand at 726.255 kb on + strand at 726.383 kb on - strand, within Psyr_0636 at 726.383 kb on - strand, within Psyr_0636 at 726.389 kb on + strand, within Psyr_0636 at 726.389 kb on + strand, within Psyr_0636 at 726.389 kb on + strand, within Psyr_0636 at 726.389 kb on + strand, within Psyr_0636 at 726.389 kb on + strand, within Psyr_0636 at 726.389 kb on + strand, within Psyr_0636 at 726.390 kb on - strand, within Psyr_0636 at 726.390 kb on - strand, within Psyr_0636 at 726.498 kb on - strand, within Psyr_0636 at 726.498 kb on - strand, within Psyr_0636 at 726.498 kb on - strand at 726.498 kb on - strand, within Psyr_0636 at 726.710 kb on - strand, within Psyr_0637 at 726.817 kb on + strand, within Psyr_0637 at 726.817 kb on + strand, within Psyr_0637 at 726.817 kb on + strand, within Psyr_0637 at 726.817 kb on + strand, within Psyr_0637 at 726.817 kb on + strand, within Psyr_0637 at 726.895 kb on + strand, within Psyr_0637 at 726.896 kb on - strand, within Psyr_0637 at 726.903 kb on + strand, within Psyr_0637 at 726.903 kb on + strand, within Psyr_0637 at 727.131 kb on - strand, within Psyr_0637 at 727.345 kb on + strand at 727.346 kb on - strand at 727.362 kb on - strand at 727.496 kb on + strand, within Psyr_0638 at 727.497 kb on - strand, within Psyr_0638 at 727.809 kb on + strand, within Psyr_0638 at 727.834 kb on - strand, within Psyr_0638 at 727.982 kb on + strand at 728.180 kb on + strand, within Psyr_0639 at 728.180 kb on + strand, within Psyr_0639 at 728.180 kb on + strand, within Psyr_0639 at 728.181 kb on - strand, within Psyr_0639 at 728.181 kb on - strand, within Psyr_0639 at 728.181 kb on - strand, within Psyr_0639 at 728.206 kb on + strand, within Psyr_0639 at 728.206 kb on + strand, within Psyr_0639 at 728.206 kb on - strand, within Psyr_0639 at 728.207 kb on - strand, within Psyr_0639 at 728.207 kb on - strand, within Psyr_0639 at 728.284 kb on + strand, within Psyr_0639 at 728.284 kb on + strand, within Psyr_0639 at 728.285 kb on - strand, within Psyr_0639
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 725,632 - +4.3 725,803 + Psyr_0635 0.42 -2.7 725,803 + Psyr_0635 0.42 +1.1 725,835 + Psyr_0635 0.49 -2.7 725,836 - Psyr_0635 0.49 -1.4 725,836 - Psyr_0635 0.49 -3.4 725,981 + Psyr_0635 0.81 +3.0 726,034 + -3.3 726,034 + -4.2 726,034 + -0.6 726,035 - +0.2 726,035 - -2.4 726,035 - +4.1 726,035 - -2.0 726,035 - -1.6 726,068 + -1.2 726,068 + +2.1 726,068 + -1.7 726,068 + +2.1 726,068 + +0.1 726,069 - -1.4 726,069 - -1.1 726,069 - +0.1 726,095 + -1.6 726,095 + +0.1 726,096 - +0.4 726,096 - -0.5 726,096 - -0.3 726,096 - -1.2 726,223 + -3.4 726,223 + -0.1 726,223 + +0.1 726,224 - +0.3 726,224 - +3.7 726,224 - -1.4 726,224 - +3.5 726,224 - -0.8 726,224 - -0.5 726,225 + +1.7 726,225 + -2.4 726,225 + -0.8 726,226 - -1.7 726,226 - -2.7 726,226 - +0.5 726,255 + -0.6 726,255 + -0.9 726,255 + +1.1 726,383 - Psyr_0636 0.31 +4.0 726,383 - Psyr_0636 0.31 +0.4 726,389 + Psyr_0636 0.32 -0.4 726,389 + Psyr_0636 0.32 +1.1 726,389 + Psyr_0636 0.32 -0.9 726,389 + Psyr_0636 0.32 +0.9 726,389 + Psyr_0636 0.32 -1.7 726,389 + Psyr_0636 0.32 -2.2 726,390 - Psyr_0636 0.33 +0.6 726,390 - Psyr_0636 0.33 -0.3 726,498 - Psyr_0636 0.62 +3.6 726,498 - Psyr_0636 0.62 -2.1 726,498 - +0.1 726,498 - Psyr_0636 0.62 +0.5 726,710 - Psyr_0637 0.11 +3.7 726,817 + Psyr_0637 0.26 -2.4 726,817 + Psyr_0637 0.26 +2.5 726,817 + Psyr_0637 0.26 +2.7 726,817 + Psyr_0637 0.26 -1.2 726,817 + Psyr_0637 0.26 -4.4 726,895 + Psyr_0637 0.37 -0.5 726,896 - Psyr_0637 0.37 -1.1 726,903 + Psyr_0637 0.38 -0.2 726,903 + Psyr_0637 0.38 -0.3 727,131 - Psyr_0637 0.70 -2.5 727,345 + +1.1 727,346 - +2.5 727,362 - +1.1 727,496 + Psyr_0638 0.23 -0.2 727,497 - Psyr_0638 0.23 -2.2 727,809 + Psyr_0638 0.76 -0.4 727,834 - Psyr_0638 0.80 -2.3 727,982 + -2.6 728,180 + Psyr_0639 0.25 -3.1 728,180 + Psyr_0639 0.25 +0.1 728,180 + Psyr_0639 0.25 -1.1 728,181 - Psyr_0639 0.25 +0.3 728,181 - Psyr_0639 0.25 +0.2 728,181 - Psyr_0639 0.25 +1.7 728,206 + Psyr_0639 0.30 -0.8 728,206 + Psyr_0639 0.30 +1.6 728,206 - Psyr_0639 0.30 -1.6 728,207 - Psyr_0639 0.30 +3.5 728,207 - Psyr_0639 0.30 -2.7 728,284 + Psyr_0639 0.44 -3.1 728,284 + Psyr_0639 0.44 +0.5 728,285 - Psyr_0639 0.44 +3.3
Or see this region's nucleotide sequence