Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0605 and Psyr_0606 overlap by 4 nucleotides Psyr_0606 and Psyr_0607 are separated by 102 nucleotides
Psyr_0605: Psyr_0605 - farnesyl-diphosphate synthase, at 695,679 to 696,566
_0605
Psyr_0606: Psyr_0606 - Exodeoxyribonuclease VII small subunit, at 696,563 to 696,808
_0606
Psyr_0607: Psyr_0607 - cystathionine beta-lyase, at 696,911 to 698,077
_0607
Position (kb) 696
697 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 695.616 kb on + strand at 695.616 kb on + strand at 695.616 kb on + strand at 695.616 kb on + strand at 695.616 kb on + strand at 695.616 kb on + strand at 695.616 kb on + strand at 695.617 kb on - strand at 696.684 kb on - strand, within Psyr_0606 at 696.894 kb on + strand at 696.894 kb on + strand at 697.206 kb on + strand, within Psyr_0607 at 697.206 kb on + strand, within Psyr_0607 at 697.320 kb on + strand, within Psyr_0607 at 697.320 kb on + strand, within Psyr_0607 at 697.343 kb on - strand, within Psyr_0607 at 697.343 kb on - strand, within Psyr_0607 at 697.375 kb on + strand, within Psyr_0607 at 697.437 kb on + strand, within Psyr_0607 at 697.437 kb on + strand, within Psyr_0607 at 697.438 kb on - strand, within Psyr_0607 at 697.438 kb on - strand, within Psyr_0607 at 697.438 kb on - strand, within Psyr_0607 at 697.438 kb on - strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.488 kb on + strand, within Psyr_0607 at 697.489 kb on - strand, within Psyr_0607 at 697.489 kb on - strand, within Psyr_0607 at 697.489 kb on - strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.608 kb on + strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.609 kb on - strand, within Psyr_0607 at 697.649 kb on - strand, within Psyr_0607 at 697.665 kb on + strand, within Psyr_0607 at 697.666 kb on - strand, within Psyr_0607 at 697.728 kb on + strand, within Psyr_0607 at 697.728 kb on + strand, within Psyr_0607 at 697.728 kb on + strand, within Psyr_0607 at 697.729 kb on - strand, within Psyr_0607
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 695,616 + +2.5 695,616 + +4.2 695,616 + +2.5 695,616 + +0.2 695,616 + +2.5 695,616 + -0.4 695,616 + -1.2 695,617 - -1.3 696,684 - Psyr_0606 0.49 -0.9 696,894 + +1.7 696,894 + +3.7 697,206 + Psyr_0607 0.25 +2.7 697,206 + Psyr_0607 0.25 -0.1 697,320 + Psyr_0607 0.35 -1.2 697,320 + Psyr_0607 0.35 +3.6 697,343 - Psyr_0607 0.37 +1.8 697,343 - Psyr_0607 0.37 +3.9 697,375 + Psyr_0607 0.40 -1.1 697,437 + Psyr_0607 0.45 +1.7 697,437 + Psyr_0607 0.45 +0.8 697,438 - Psyr_0607 0.45 +0.9 697,438 - Psyr_0607 0.45 +0.1 697,438 - Psyr_0607 0.45 +0.0 697,438 - Psyr_0607 0.45 +0.3 697,488 + Psyr_0607 0.49 -1.9 697,488 + Psyr_0607 0.49 -1.9 697,488 + Psyr_0607 0.49 -0.3 697,488 + Psyr_0607 0.49 +0.5 697,488 + Psyr_0607 0.49 +1.7 697,488 + Psyr_0607 0.49 +3.8 697,488 + Psyr_0607 0.49 -4.0 697,489 - Psyr_0607 0.50 +2.1 697,489 - Psyr_0607 0.50 -0.1 697,489 - Psyr_0607 0.50 +1.7 697,608 + Psyr_0607 0.60 +0.2 697,608 + Psyr_0607 0.60 +2.1 697,608 + Psyr_0607 0.60 +2.9 697,608 + Psyr_0607 0.60 -0.3 697,608 + Psyr_0607 0.60 -0.4 697,609 - Psyr_0607 0.60 -2.3 697,609 - Psyr_0607 0.60 -0.0 697,609 - Psyr_0607 0.60 -1.0 697,609 - Psyr_0607 0.60 +0.1 697,609 - Psyr_0607 0.60 +2.5 697,609 - Psyr_0607 0.60 +2.5 697,649 - Psyr_0607 0.63 +2.9 697,665 + Psyr_0607 0.65 -1.3 697,666 - Psyr_0607 0.65 +0.3 697,728 + Psyr_0607 0.70 +0.2 697,728 + Psyr_0607 0.70 +2.5 697,728 + Psyr_0607 0.70 +4.1 697,729 - Psyr_0607 0.70 -1.2
Or see this region's nucleotide sequence