Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0580 and Psyr_0581 are separated by 257 nucleotides Psyr_0581 and Psyr_0582 are separated by 28 nucleotides Psyr_0582 and Psyr_0583 are separated by 39 nucleotides Psyr_0583 and Psyr_0584 are separated by 19 nucleotides Psyr_0584 and Psyr_0585 are separated by 123 nucleotides
Psyr_0580: Psyr_0580 - 23S rRNA Gm-2251 2'-O-methyltransferase, at 662,568 to 663,323
_0580
Psyr_0581: Psyr_0581 - SSU ribosomal protein S6P, at 663,581 to 664,006
_0581
Psyr_0582: Psyr_0582 - SSU ribosomal protein S18P, at 664,035 to 664,265
_0582
Psyr_0583: Psyr_0583 - membrane protein, putative, at 664,305 to 665,201
_0583
Psyr_0584: Psyr_0584 - LSU ribosomal protein L9P, at 665,221 to 665,667
_0584
Psyr_0585: Psyr_0585 - primary replicative DNA helicase, at 665,791 to 667,185
_0585
Position (kb) 664
665
666 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 663.321 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.324 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.352 kb on + strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.353 kb on - strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.359 kb on + strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.360 kb on - strand at 663.376 kb on + strand at 663.376 kb on + strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.377 kb on - strand at 663.382 kb on + strand at 663.383 kb on - strand at 663.383 kb on - strand at 663.421 kb on + strand at 663.422 kb on - strand at 663.422 kb on - strand at 664.614 kb on + strand, within Psyr_0583 at 664.614 kb on + strand, within Psyr_0583 at 664.614 kb on + strand, within Psyr_0583 at 664.621 kb on + strand, within Psyr_0583 at 664.723 kb on + strand, within Psyr_0583 at 664.723 kb on + strand, within Psyr_0583 at 664.723 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.845 kb on + strand, within Psyr_0583 at 664.964 kb on + strand, within Psyr_0583 at 664.964 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.064 kb on + strand, within Psyr_0583 at 665.113 kb on + strand at 665.206 kb on + strand at 665.301 kb on + strand, within Psyr_0584 at 665.305 kb on + strand, within Psyr_0584 at 665.454 kb on + strand, within Psyr_0584 at 665.630 kb on + strand at 665.665 kb on + strand at 665.676 kb on + strand at 665.729 kb on + strand at 665.729 kb on + strand at 665.729 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 663,321 + +1.1 663,324 + -1.3 663,324 + -0.9 663,324 + +1.7 663,324 + +2.8 663,352 + -1.2 663,352 + +0.3 663,352 + -0.7 663,352 + +3.3 663,352 + +2.1 663,352 + -3.3 663,353 - -0.1 663,353 - +2.5 663,353 - -1.9 663,353 - -1.6 663,359 + +0.7 663,359 + +2.2 663,359 + +2.5 663,359 + +2.7 663,360 - -3.6 663,360 - +3.1 663,360 - +2.5 663,360 - +0.1 663,360 - -1.1 663,360 - -1.7 663,360 - +0.1 663,376 + +2.5 663,376 + +4.0 663,377 - +0.1 663,377 - +3.5 663,377 - -0.1 663,377 - -3.0 663,377 - +1.1 663,382 + -1.1 663,383 - -1.0 663,383 - -1.5 663,421 + +2.7 663,422 - -0.4 663,422 - +3.1 664,614 + Psyr_0583 0.34 -1.9 664,614 + Psyr_0583 0.34 -2.3 664,614 + Psyr_0583 0.34 +2.9 664,621 + Psyr_0583 0.35 +2.1 664,723 + Psyr_0583 0.47 +0.1 664,723 + Psyr_0583 0.47 -1.7 664,723 + Psyr_0583 0.47 +2.1 664,845 + Psyr_0583 0.60 -1.9 664,845 + Psyr_0583 0.60 +2.1 664,845 + Psyr_0583 0.60 +0.2 664,845 + Psyr_0583 0.60 -1.3 664,845 + Psyr_0583 0.60 -4.0 664,845 + Psyr_0583 0.60 -2.4 664,964 + Psyr_0583 0.73 -1.7 664,964 + Psyr_0583 0.73 +0.1 665,064 + Psyr_0583 0.85 -1.1 665,064 + Psyr_0583 0.85 +0.4 665,064 + Psyr_0583 0.85 -1.0 665,064 + Psyr_0583 0.85 -0.8 665,064 + Psyr_0583 0.85 -0.2 665,064 + Psyr_0583 0.85 +3.1 665,064 + Psyr_0583 0.85 -1.2 665,064 + Psyr_0583 0.85 -2.4 665,064 + Psyr_0583 0.85 +2.5 665,064 + Psyr_0583 0.85 -1.7 665,064 + Psyr_0583 0.85 -0.8 665,064 + Psyr_0583 0.85 +4.7 665,064 + Psyr_0583 0.85 -1.7 665,064 + Psyr_0583 0.85 +1.1 665,064 + Psyr_0583 0.85 -0.7 665,064 + Psyr_0583 0.85 -0.6 665,113 + -1.9 665,206 + +3.5 665,301 + Psyr_0584 0.18 +2.1 665,305 + Psyr_0584 0.19 -4.0 665,454 + Psyr_0584 0.52 -0.9 665,630 + -2.2 665,665 + +1.7 665,676 + -3.3 665,729 + -2.0 665,729 + -0.0 665,729 + -0.8
Or see this region's nucleotide sequence