Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0565 and Psyr_0566 are separated by 736 nucleotides Psyr_0566 and Psyr_0567 are separated by 63 nucleotides
Psyr_0565: Psyr_0565 - Protein of unknown function UPF0126, at 641,729 to 642,340
_0565
Psyr_0566: Psyr_0566 - 4Fe-4S cluster binding protein, at 643,077 to 644,165
_0566
Psyr_0567: Psyr_0567 - Protein of unknown function UPF0031:YjeF-related protein, N-terminal, at 644,229 to 645,719
_0567
Position (kb)
643
644
645 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 642.170 kb on + strand, within Psyr_0565 at 642.171 kb on - strand, within Psyr_0565 at 642.210 kb on + strand, within Psyr_0565 at 642.211 kb on - strand, within Psyr_0565 at 642.218 kb on + strand, within Psyr_0565 at 642.219 kb on - strand, within Psyr_0565 at 642.323 kb on + strand at 642.324 kb on - strand at 642.324 kb on - strand at 642.324 kb on - strand at 642.325 kb on + strand at 642.326 kb on - strand at 642.376 kb on + strand at 642.376 kb on + strand at 642.376 kb on + strand at 642.376 kb on + strand at 642.376 kb on + strand at 642.377 kb on - strand at 642.377 kb on - strand at 642.377 kb on - strand at 642.408 kb on - strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.471 kb on - strand at 642.471 kb on - strand at 642.471 kb on - strand at 642.471 kb on - strand at 642.471 kb on - strand at 642.471 kb on - strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.841 kb on + strand at 642.841 kb on + strand at 642.841 kb on + strand at 642.841 kb on + strand at 642.841 kb on + strand at 642.841 kb on + strand at 642.841 kb on + strand at 642.843 kb on + strand at 642.843 kb on + strand at 642.845 kb on + strand at 642.845 kb on + strand at 642.845 kb on + strand at 642.905 kb on - strand at 642.905 kb on - strand at 642.905 kb on - strand at 642.905 kb on - strand at 642.905 kb on - strand at 643.018 kb on + strand at 643.018 kb on + strand at 643.018 kb on + strand at 643.018 kb on + strand at 643.018 kb on + strand at 643.018 kb on + strand at 643.020 kb on + strand at 643.020 kb on + strand at 643.020 kb on + strand at 643.020 kb on + strand at 643.020 kb on + strand at 643.022 kb on + strand at 643.022 kb on + strand at 643.022 kb on + strand at 643.022 kb on + strand at 643.022 kb on + strand at 643.022 kb on + strand at 643.022 kb on + strand at 643.061 kb on + strand at 643.072 kb on + strand at 643.078 kb on + strand at 643.078 kb on + strand at 643.235 kb on - strand, within Psyr_0566 at 643.484 kb on + strand, within Psyr_0566 at 643.484 kb on + strand, within Psyr_0566 at 643.484 kb on + strand, within Psyr_0566 at 643.484 kb on + strand, within Psyr_0566 at 643.484 kb on + strand, within Psyr_0566 at 643.485 kb on - strand, within Psyr_0566 at 643.485 kb on - strand, within Psyr_0566 at 643.507 kb on + strand, within Psyr_0566 at 643.508 kb on - strand, within Psyr_0566 at 643.553 kb on + strand, within Psyr_0566 at 643.554 kb on - strand, within Psyr_0566 at 643.554 kb on - strand, within Psyr_0566 at 643.772 kb on + strand, within Psyr_0566 at 643.772 kb on + strand, within Psyr_0566 at 643.773 kb on - strand, within Psyr_0566 at 643.816 kb on + strand, within Psyr_0566 at 643.817 kb on - strand, within Psyr_0566 at 643.846 kb on + strand, within Psyr_0566 at 643.846 kb on + strand, within Psyr_0566 at 643.847 kb on - strand, within Psyr_0566 at 643.847 kb on - strand, within Psyr_0566 at 643.847 kb on - strand, within Psyr_0566 at 643.847 kb on - strand, within Psyr_0566 at 643.900 kb on + strand, within Psyr_0566 at 643.900 kb on + strand, within Psyr_0566 at 643.900 kb on + strand, within Psyr_0566 at 643.901 kb on - strand, within Psyr_0566 at 643.901 kb on - strand, within Psyr_0566 at 643.901 kb on - strand, within Psyr_0566 at 643.901 kb on - strand, within Psyr_0566 at 643.982 kb on + strand, within Psyr_0566 at 643.983 kb on - strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.984 kb on + strand, within Psyr_0566 at 643.985 kb on - strand, within Psyr_0566 at 643.985 kb on - strand, within Psyr_0566 at 643.985 kb on - strand, within Psyr_0566 at 643.985 kb on - strand, within Psyr_0566 at 643.985 kb on - strand, within Psyr_0566 at 643.985 kb on - strand, within Psyr_0566 at 644.002 kb on + strand, within Psyr_0566 at 644.002 kb on + strand, within Psyr_0566 at 644.145 kb on + strand at 644.145 kb on + strand at 644.145 kb on + strand at 644.145 kb on + strand at 644.145 kb on + strand at 644.146 kb on - strand at 644.146 kb on - strand at 644.146 kb on - strand at 644.146 kb on - strand at 644.146 kb on - strand at 644.146 kb on - strand at 644.153 kb on + strand at 644.153 kb on + strand at 644.154 kb on - strand at 644.154 kb on - strand at 644.195 kb on + strand at 644.195 kb on + strand at 644.195 kb on + strand at 644.196 kb on - strand at 644.196 kb on - strand at 644.196 kb on - strand at 644.197 kb on + strand at 644.197 kb on + strand at 644.197 kb on + strand at 644.197 kb on + strand at 644.197 kb on + strand at 644.197 kb on + strand at 644.198 kb on - strand at 644.198 kb on - strand at 644.222 kb on + strand at 644.240 kb on + strand at 644.241 kb on - strand at 644.252 kb on - strand at 644.265 kb on + strand at 644.265 kb on + strand at 644.368 kb on + strand at 644.368 kb on + strand at 644.559 kb on - strand, within Psyr_0567 at 644.646 kb on + strand, within Psyr_0567 at 644.646 kb on + strand, within Psyr_0567 at 644.646 kb on + strand, within Psyr_0567 at 644.647 kb on - strand, within Psyr_0567 at 644.647 kb on - strand, within Psyr_0567 at 644.647 kb on - strand, within Psyr_0567 at 644.647 kb on - strand, within Psyr_0567 at 644.771 kb on + strand, within Psyr_0567
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 642,170 + Psyr_0565 0.72 -1.0 642,171 - Psyr_0565 0.72 +0.1 642,210 + Psyr_0565 0.79 -1.8 642,211 - Psyr_0565 0.79 +1.1 642,218 + Psyr_0565 0.80 +2.1 642,219 - Psyr_0565 0.80 -3.4 642,323 + +0.6 642,324 - +3.1 642,324 - -3.0 642,324 - +0.3 642,325 + -0.6 642,326 - -3.2 642,376 + -0.1 642,376 + -1.2 642,376 + -0.5 642,376 + -2.1 642,376 + -0.7 642,377 - +2.5 642,377 - -2.5 642,377 - -0.5 642,408 - +1.0 642,415 + -1.5 642,415 + -1.2 642,415 + +2.7 642,415 + +2.5 642,415 + +2.7 642,415 + -2.0 642,415 + -1.3 642,415 + -0.3 642,415 + +4.0 642,415 + -2.5 642,415 + +2.1 642,415 + +2.9 642,416 - -0.5 642,416 - +0.1 642,416 - -3.3 642,416 - -2.2 642,416 - -0.7 642,416 - -1.2 642,416 - +0.5 642,416 - -1.2 642,416 - -0.6 642,470 + -0.0 642,470 + -1.2 642,470 + -2.3 642,470 + +4.5 642,470 + -0.5 642,470 + +3.1 642,470 + -1.7 642,470 + +2.0 642,470 + +1.9 642,471 - -0.3 642,471 - -1.4 642,471 - -1.7 642,471 - +3.7 642,471 - -0.3 642,471 - +0.7 642,727 + -1.3 642,727 + -0.3 642,727 + +2.5 642,727 + +2.5 642,727 + -1.4 642,727 + +3.5 642,727 + +1.7 642,727 + +0.3 642,727 + +0.3 642,727 + -2.0 642,727 + +3.5 642,727 + -1.1 642,727 + -0.0 642,727 + +0.1 642,841 + -2.9 642,841 + +2.5 642,841 + +3.1 642,841 + -1.8 642,841 + +1.7 642,841 + -0.6 642,841 + +1.7 642,843 + -0.1 642,843 + -1.7 642,845 + -2.1 642,845 + -0.5 642,845 + -0.1 642,905 - -3.0 642,905 - -0.1 642,905 - +1.2 642,905 - +1.1 642,905 - -0.2 643,018 + -1.0 643,018 + -1.1 643,018 + -0.9 643,018 + -0.2 643,018 + -0.8 643,018 + +1.7 643,020 + -1.3 643,020 + +1.1 643,020 + -0.5 643,020 + -2.5 643,020 + -1.9 643,022 + -0.9 643,022 + +2.5 643,022 + -0.4 643,022 + -0.8 643,022 + -0.7 643,022 + +2.1 643,022 + -1.5 643,061 + +1.7 643,072 + +3.5 643,078 + -0.9 643,078 + +0.3 643,235 - Psyr_0566 0.15 -1.2 643,484 + Psyr_0566 0.37 +2.5 643,484 + Psyr_0566 0.37 +1.7 643,484 + Psyr_0566 0.37 +2.1 643,484 + Psyr_0566 0.37 -0.6 643,484 + Psyr_0566 0.37 +3.7 643,485 - Psyr_0566 0.37 +2.7 643,485 - Psyr_0566 0.37 +3.9 643,507 + Psyr_0566 0.39 +3.7 643,508 - Psyr_0566 0.40 -1.3 643,553 + Psyr_0566 0.44 +0.1 643,554 - Psyr_0566 0.44 +2.1 643,554 - Psyr_0566 0.44 -0.8 643,772 + Psyr_0566 0.64 -0.5 643,772 + Psyr_0566 0.64 +1.7 643,773 - Psyr_0566 0.64 -2.3 643,816 + Psyr_0566 0.68 -1.0 643,817 - Psyr_0566 0.68 +0.8 643,846 + Psyr_0566 0.71 +3.1 643,846 + Psyr_0566 0.71 +2.9 643,847 - Psyr_0566 0.71 +2.9 643,847 - Psyr_0566 0.71 -0.2 643,847 - Psyr_0566 0.71 -0.2 643,847 - Psyr_0566 0.71 -0.9 643,900 + Psyr_0566 0.76 -0.9 643,900 + Psyr_0566 0.76 -1.1 643,900 + Psyr_0566 0.76 -1.9 643,901 - Psyr_0566 0.76 +1.7 643,901 - Psyr_0566 0.76 +3.6 643,901 - Psyr_0566 0.76 -0.6 643,901 - Psyr_0566 0.76 +0.7 643,982 + Psyr_0566 0.83 -1.5 643,983 - Psyr_0566 0.83 +0.6 643,984 + Psyr_0566 0.83 +1.5 643,984 + Psyr_0566 0.83 +0.4 643,984 + Psyr_0566 0.83 -0.1 643,984 + Psyr_0566 0.83 +3.7 643,984 + Psyr_0566 0.83 -0.5 643,984 + Psyr_0566 0.83 -0.9 643,984 + Psyr_0566 0.83 -2.7 643,984 + Psyr_0566 0.83 +0.1 643,984 + Psyr_0566 0.83 +2.5 643,984 + Psyr_0566 0.83 +0.0 643,984 + Psyr_0566 0.83 +3.5 643,984 + Psyr_0566 0.83 +2.1 643,984 + Psyr_0566 0.83 +0.2 643,985 - Psyr_0566 0.83 +2.1 643,985 - Psyr_0566 0.83 +3.3 643,985 - Psyr_0566 0.83 -0.1 643,985 - Psyr_0566 0.83 -0.9 643,985 - Psyr_0566 0.83 -3.5 643,985 - Psyr_0566 0.83 -2.3 644,002 + Psyr_0566 0.85 +3.8 644,002 + Psyr_0566 0.85 -1.3 644,145 + -1.1 644,145 + -0.2 644,145 + +0.4 644,145 + -0.9 644,145 + -2.2 644,146 - -2.3 644,146 - -1.0 644,146 - +2.7 644,146 - -0.4 644,146 - +1.7 644,146 - +0.0 644,153 + -2.8 644,153 + +2.5 644,154 - -1.5 644,154 - +0.7 644,195 + -1.4 644,195 + +0.1 644,195 + -1.2 644,196 - +1.5 644,196 - -2.9 644,196 - -0.5 644,197 + +2.7 644,197 + -0.5 644,197 + +2.5 644,197 + +2.7 644,197 + +0.1 644,197 + -3.4 644,198 - -1.6 644,198 - +0.1 644,222 + +2.1 644,240 + +4.5 644,241 - +2.5 644,252 - -1.1 644,265 + -2.5 644,265 + -2.5 644,368 + +2.7 644,368 + -1.5 644,559 - Psyr_0567 0.22 -1.5 644,646 + Psyr_0567 0.28 +1.1 644,646 + Psyr_0567 0.28 -0.6 644,646 + Psyr_0567 0.28 +0.9 644,647 - Psyr_0567 0.28 +2.9 644,647 - Psyr_0567 0.28 -0.8 644,647 - Psyr_0567 0.28 +2.1 644,647 - Psyr_0567 0.28 +2.5 644,771 + Psyr_0567 0.36 +2.5
Or see this region's nucleotide sequence