Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0563 and Psyr_0564 are separated by 108 nucleotides Psyr_0564 and Psyr_0565 are separated by 96 nucleotides Psyr_0565 and Psyr_0566 are separated by 736 nucleotides
Psyr_0563: Psyr_0563 - Protein of unknown function DUF258, at 639,956 to 640,987
_0563
Psyr_0564: Psyr_0564 - Exonuclease, at 641,096 to 641,632
_0564
Psyr_0565: Psyr_0565 - Protein of unknown function UPF0126, at 641,729 to 642,340
_0565
Psyr_0566: Psyr_0566 - 4Fe-4S cluster binding protein, at 643,077 to 644,165
_0566
Position (kb)
641
642
643 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 641.007 kb on - strand at 641.007 kb on - strand at 641.018 kb on + strand at 641.019 kb on - strand at 641.019 kb on - strand at 641.046 kb on + strand at 641.046 kb on + strand at 641.046 kb on + strand at 641.064 kb on + strand at 641.064 kb on + strand at 641.065 kb on - strand at 641.077 kb on + strand at 641.078 kb on - strand at 641.657 kb on - strand at 641.657 kb on - strand at 641.657 kb on - strand at 641.697 kb on - strand at 641.744 kb on + strand at 641.745 kb on - strand at 641.745 kb on - strand at 641.745 kb on - strand at 641.745 kb on - strand at 641.755 kb on + strand at 641.871 kb on + strand, within Psyr_0565 at 641.871 kb on + strand, within Psyr_0565 at 641.871 kb on + strand, within Psyr_0565 at 641.871 kb on + strand, within Psyr_0565 at 641.872 kb on - strand, within Psyr_0565 at 641.872 kb on - strand, within Psyr_0565 at 641.915 kb on + strand, within Psyr_0565 at 641.916 kb on - strand, within Psyr_0565 at 642.025 kb on + strand, within Psyr_0565 at 642.025 kb on + strand, within Psyr_0565 at 642.040 kb on + strand, within Psyr_0565 at 642.040 kb on + strand, within Psyr_0565 at 642.041 kb on - strand, within Psyr_0565 at 642.170 kb on + strand, within Psyr_0565 at 642.171 kb on - strand, within Psyr_0565 at 642.210 kb on + strand, within Psyr_0565 at 642.211 kb on - strand, within Psyr_0565 at 642.218 kb on + strand, within Psyr_0565 at 642.219 kb on - strand, within Psyr_0565 at 642.323 kb on + strand at 642.324 kb on - strand at 642.324 kb on - strand at 642.324 kb on - strand at 642.325 kb on + strand at 642.326 kb on - strand at 642.376 kb on + strand at 642.376 kb on + strand at 642.376 kb on + strand at 642.376 kb on + strand at 642.376 kb on + strand at 642.377 kb on - strand at 642.377 kb on - strand at 642.377 kb on - strand at 642.408 kb on - strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.415 kb on + strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.416 kb on - strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.470 kb on + strand at 642.471 kb on - strand at 642.471 kb on - strand at 642.471 kb on - strand at 642.471 kb on - strand at 642.471 kb on - strand at 642.471 kb on - strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.727 kb on + strand at 642.841 kb on + strand at 642.841 kb on + strand at 642.841 kb on + strand at 642.841 kb on + strand at 642.841 kb on + strand at 642.841 kb on + strand at 642.841 kb on + strand at 642.843 kb on + strand at 642.843 kb on + strand at 642.845 kb on + strand at 642.845 kb on + strand at 642.845 kb on + strand at 642.905 kb on - strand at 642.905 kb on - strand at 642.905 kb on - strand at 642.905 kb on - strand at 642.905 kb on - strand at 643.018 kb on + strand at 643.018 kb on + strand at 643.018 kb on + strand at 643.018 kb on + strand at 643.018 kb on + strand at 643.018 kb on + strand at 643.020 kb on + strand at 643.020 kb on + strand at 643.020 kb on + strand at 643.020 kb on + strand at 643.020 kb on + strand at 643.022 kb on + strand at 643.022 kb on + strand at 643.022 kb on + strand at 643.022 kb on + strand at 643.022 kb on + strand at 643.022 kb on + strand at 643.022 kb on + strand at 643.061 kb on + strand at 643.072 kb on + strand at 643.078 kb on + strand at 643.078 kb on + strand at 643.235 kb on - strand, within Psyr_0566
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 641,007 - -1.8 641,007 - +0.7 641,018 + +2.7 641,019 - -0.4 641,019 - +2.1 641,046 + -0.5 641,046 + -1.0 641,046 + +1.7 641,064 + +0.1 641,064 + +2.1 641,065 - -0.0 641,077 + +1.7 641,078 - +0.1 641,657 - -0.0 641,657 - -1.1 641,657 - -0.0 641,697 - -2.5 641,744 + +0.5 641,745 - +2.5 641,745 - -1.8 641,745 - +0.1 641,745 - +1.7 641,755 + -0.8 641,871 + Psyr_0565 0.23 +0.1 641,871 + Psyr_0565 0.23 -1.4 641,871 + Psyr_0565 0.23 +1.7 641,871 + Psyr_0565 0.23 -4.0 641,872 - Psyr_0565 0.23 -1.1 641,872 - Psyr_0565 0.23 -1.9 641,915 + Psyr_0565 0.30 -0.6 641,916 - Psyr_0565 0.31 -2.9 642,025 + Psyr_0565 0.48 +3.1 642,025 + Psyr_0565 0.48 -1.1 642,040 + Psyr_0565 0.51 +0.1 642,040 + Psyr_0565 0.51 -4.0 642,041 - Psyr_0565 0.51 -2.9 642,170 + Psyr_0565 0.72 -1.0 642,171 - Psyr_0565 0.72 +0.1 642,210 + Psyr_0565 0.79 -1.8 642,211 - Psyr_0565 0.79 +1.1 642,218 + Psyr_0565 0.80 +2.1 642,219 - Psyr_0565 0.80 -3.4 642,323 + +0.6 642,324 - +3.1 642,324 - -3.0 642,324 - +0.3 642,325 + -0.6 642,326 - -3.2 642,376 + -0.1 642,376 + -1.2 642,376 + -0.5 642,376 + -2.1 642,376 + -0.7 642,377 - +2.5 642,377 - -2.5 642,377 - -0.5 642,408 - +1.0 642,415 + -1.5 642,415 + -1.2 642,415 + +2.7 642,415 + +2.5 642,415 + +2.7 642,415 + -2.0 642,415 + -1.3 642,415 + -0.3 642,415 + +4.0 642,415 + -2.5 642,415 + +2.1 642,415 + +2.9 642,416 - -0.5 642,416 - +0.1 642,416 - -3.3 642,416 - -2.2 642,416 - -0.7 642,416 - -1.2 642,416 - +0.5 642,416 - -1.2 642,416 - -0.6 642,470 + -0.0 642,470 + -1.2 642,470 + -2.3 642,470 + +4.5 642,470 + -0.5 642,470 + +3.1 642,470 + -1.7 642,470 + +2.0 642,470 + +1.9 642,471 - -0.3 642,471 - -1.4 642,471 - -1.7 642,471 - +3.7 642,471 - -0.3 642,471 - +0.7 642,727 + -1.3 642,727 + -0.3 642,727 + +2.5 642,727 + +2.5 642,727 + -1.4 642,727 + +3.5 642,727 + +1.7 642,727 + +0.3 642,727 + +0.3 642,727 + -2.0 642,727 + +3.5 642,727 + -1.1 642,727 + -0.0 642,727 + +0.1 642,841 + -2.9 642,841 + +2.5 642,841 + +3.1 642,841 + -1.8 642,841 + +1.7 642,841 + -0.6 642,841 + +1.7 642,843 + -0.1 642,843 + -1.7 642,845 + -2.1 642,845 + -0.5 642,845 + -0.1 642,905 - -3.0 642,905 - -0.1 642,905 - +1.2 642,905 - +1.1 642,905 - -0.2 643,018 + -1.0 643,018 + -1.1 643,018 + -0.9 643,018 + -0.2 643,018 + -0.8 643,018 + +1.7 643,020 + -1.3 643,020 + +1.1 643,020 + -0.5 643,020 + -2.5 643,020 + -1.9 643,022 + -0.9 643,022 + +2.5 643,022 + -0.4 643,022 + -0.8 643,022 + -0.7 643,022 + +2.1 643,022 + -1.5 643,061 + +1.7 643,072 + +3.5 643,078 + -0.9 643,078 + +0.3 643,235 - Psyr_0566 0.15 -1.2
Or see this region's nucleotide sequence