Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0549 and Psyr_0550 are separated by 88 nucleotides Psyr_0550 and Psyr_0551 are separated by 76 nucleotides
Psyr_0549: Psyr_0549 - Metallophosphoesterase, at 620,912 to 621,718
_0549
Psyr_0550: Psyr_0550 - Protein of unknown function UPF0227, at 621,807 to 622,424
_0550
Psyr_0551: Psyr_0551 - DNA topoisomerase IV subunit B, at 622,501 to 624,405
_0551
Position (kb) 621
622
623 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 621.117 kb on - strand, within Psyr_0549 at 621.448 kb on - strand, within Psyr_0549 at 621.486 kb on + strand, within Psyr_0549 at 621.733 kb on - strand at 621.768 kb on + strand at 621.773 kb on + strand at 621.773 kb on + strand at 621.774 kb on - strand at 621.774 kb on - strand at 621.774 kb on - strand at 621.774 kb on - strand at 621.774 kb on - strand at 621.832 kb on + strand at 621.833 kb on - strand at 621.833 kb on - strand at 621.833 kb on - strand at 621.833 kb on - strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.834 kb on + strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 621.835 kb on - strand at 622.030 kb on - strand, within Psyr_0550 at 622.032 kb on + strand, within Psyr_0550 at 622.032 kb on + strand, within Psyr_0550 at 622.032 kb on + strand, within Psyr_0550 at 622.140 kb on + strand, within Psyr_0550 at 622.287 kb on + strand, within Psyr_0550 at 622.422 kb on + strand at 622.422 kb on + strand at 622.422 kb on + strand at 622.422 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 621,117 - Psyr_0549 0.25 -3.0 621,448 - Psyr_0549 0.66 +3.8 621,486 + Psyr_0549 0.71 +3.3 621,733 - -1.0 621,768 + +1.1 621,773 + +1.0 621,773 + -0.6 621,774 - +1.1 621,774 - -1.0 621,774 - +0.8 621,774 - +1.1 621,774 - -1.5 621,832 + +3.7 621,833 - +2.7 621,833 - -0.9 621,833 - -1.3 621,833 - +0.5 621,834 + -1.9 621,834 + -1.5 621,834 + -0.2 621,834 + +2.7 621,834 + +2.9 621,834 + -1.4 621,834 + -2.3 621,834 + +2.5 621,834 + +0.0 621,834 + -0.9 621,834 + -0.3 621,834 + +1.1 621,834 + -3.5 621,834 + +3.8 621,834 + -1.0 621,834 + -1.8 621,834 + -1.1 621,834 + +0.6 621,834 + -3.6 621,835 - +0.7 621,835 - -1.7 621,835 - +1.2 621,835 - -2.1 621,835 - +3.1 621,835 - +0.1 621,835 - -0.6 621,835 - -2.9 621,835 - -0.1 621,835 - +1.0 621,835 - +1.2 621,835 - +0.2 621,835 - -2.1 621,835 - -0.6 621,835 - -1.0 621,835 - +3.1 621,835 - -0.2 621,835 - +0.2 621,835 - -0.7 621,835 - +3.9 621,835 - +0.5 621,835 - -2.0 621,835 - -2.6 621,835 - -2.4 621,835 - -0.8 621,835 - -1.5 621,835 - +2.9 621,835 - +1.7 621,835 - +0.6 621,835 - -1.4 622,030 - Psyr_0550 0.36 -4.3 622,032 + Psyr_0550 0.36 -1.4 622,032 + Psyr_0550 0.36 -1.8 622,032 + Psyr_0550 0.36 -0.8 622,140 + Psyr_0550 0.54 -2.0 622,287 + Psyr_0550 0.78 -2.1 622,422 + -0.4 622,422 + +0.6 622,422 + +3.9 622,422 + -3.5
Or see this region's nucleotide sequence