Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0514 and Psyr_0515 are separated by 137 nucleotides Psyr_0515 and Psyr_0516 are separated by 175 nucleotides
Psyr_0514: Psyr_0514 - Intergral membrane protein, YccS:Integral membrane protein, YccS/YhfK, at 569,076 to 571,274
_0514
Psyr_0515: Psyr_0515 - HI0933-like protein, at 571,412 to 572,590
_0515
Psyr_0516: Psyr_0516 - ATP-dependent RNA helicase DbpA, at 572,766 to 574,187
_0516
Position (kb)
571
572
573 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 570.478 kb on + strand, within Psyr_0514 at 570.478 kb on + strand, within Psyr_0514 at 570.478 kb on + strand, within Psyr_0514 at 570.479 kb on - strand, within Psyr_0514 at 570.484 kb on + strand, within Psyr_0514 at 570.529 kb on + strand, within Psyr_0514 at 570.529 kb on + strand, within Psyr_0514 at 570.529 kb on + strand, within Psyr_0514 at 570.529 kb on + strand, within Psyr_0514 at 570.530 kb on - strand, within Psyr_0514 at 570.530 kb on - strand, within Psyr_0514 at 570.852 kb on + strand, within Psyr_0514 at 570.853 kb on - strand, within Psyr_0514 at 570.853 kb on - strand, within Psyr_0514 at 570.853 kb on - strand, within Psyr_0514 at 570.853 kb on - strand, within Psyr_0514 at 570.889 kb on + strand, within Psyr_0514 at 570.889 kb on + strand, within Psyr_0514 at 570.928 kb on + strand, within Psyr_0514 at 570.928 kb on + strand, within Psyr_0514 at 571.211 kb on + strand at 571.211 kb on + strand at 571.213 kb on + strand at 571.213 kb on + strand at 571.213 kb on + strand at 571.213 kb on + strand at 571.214 kb on - strand at 571.214 kb on - strand at 571.214 kb on - strand at 571.214 kb on - strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.323 kb on + strand at 571.323 kb on - strand at 571.324 kb on - strand at 571.324 kb on - strand at 571.324 kb on - strand at 571.324 kb on - strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.348 kb on + strand at 571.348 kb on + strand at 571.348 kb on + strand at 571.348 kb on + strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.370 kb on + strand at 571.398 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.434 kb on + strand at 571.434 kb on + strand at 571.435 kb on - strand at 571.671 kb on + strand, within Psyr_0515 at 571.671 kb on + strand, within Psyr_0515 at 571.672 kb on - strand, within Psyr_0515 at 571.672 kb on - strand, within Psyr_0515 at 571.672 kb on - strand, within Psyr_0515 at 572.424 kb on + strand, within Psyr_0515 at 572.424 kb on + strand, within Psyr_0515 at 572.424 kb on + strand, within Psyr_0515 at 572.424 kb on + strand, within Psyr_0515 at 572.424 kb on + strand, within Psyr_0515 at 572.424 kb on + strand, within Psyr_0515 at 572.424 kb on + strand, within Psyr_0515 at 572.424 kb on + strand, within Psyr_0515 at 572.425 kb on - strand, within Psyr_0515 at 572.425 kb on - strand, within Psyr_0515 at 572.428 kb on + strand, within Psyr_0515 at 572.428 kb on + strand, within Psyr_0515 at 572.428 kb on + strand, within Psyr_0515 at 572.428 kb on + strand, within Psyr_0515 at 572.428 kb on + strand, within Psyr_0515 at 572.428 kb on + strand, within Psyr_0515 at 572.429 kb on - strand, within Psyr_0515 at 572.429 kb on - strand, within Psyr_0515 at 572.429 kb on - strand, within Psyr_0515 at 572.429 kb on - strand, within Psyr_0515 at 572.429 kb on - strand, within Psyr_0515 at 572.429 kb on - strand, within Psyr_0515 at 572.429 kb on - strand, within Psyr_0515 at 572.429 kb on - strand, within Psyr_0515 at 572.429 kb on - strand, within Psyr_0515 at 572.570 kb on + strand at 572.570 kb on + strand at 572.570 kb on + strand at 572.571 kb on - strand at 572.571 kb on - strand at 572.571 kb on - strand at 572.644 kb on + strand at 572.644 kb on + strand at 572.645 kb on - strand at 572.645 kb on - strand at 572.645 kb on - strand at 572.645 kb on - strand at 572.715 kb on + strand at 572.715 kb on + strand at 572.715 kb on + strand at 572.715 kb on + strand at 572.715 kb on + strand at 572.715 kb on + strand at 572.715 kb on + strand at 572.715 kb on + strand at 572.715 kb on + strand at 572.715 kb on + strand at 572.715 kb on + strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.716 kb on - strand at 572.717 kb on + strand at 572.717 kb on + strand at 572.718 kb on - strand at 572.718 kb on - strand at 572.718 kb on - strand at 572.718 kb on - strand at 572.718 kb on - strand at 572.718 kb on - strand at 572.718 kb on - strand at 572.738 kb on + strand at 572.768 kb on - strand at 572.861 kb on - strand at 573.269 kb on + strand, within Psyr_0516 at 573.270 kb on - strand, within Psyr_0516 at 573.295 kb on - strand, within Psyr_0516 at 573.438 kb on + strand, within Psyr_0516 at 573.439 kb on - strand, within Psyr_0516
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 570,478 + Psyr_0514 0.64 -0.1 570,478 + Psyr_0514 0.64 +0.1 570,478 + Psyr_0514 0.64 +0.4 570,479 - Psyr_0514 0.64 -1.7 570,484 + Psyr_0514 0.64 -0.2 570,529 + Psyr_0514 0.66 -1.0 570,529 + Psyr_0514 0.66 +2.9 570,529 + Psyr_0514 0.66 +0.5 570,529 + Psyr_0514 0.66 +1.3 570,530 - Psyr_0514 0.66 +4.0 570,530 - Psyr_0514 0.66 +0.4 570,852 + Psyr_0514 0.81 -0.8 570,853 - Psyr_0514 0.81 +3.5 570,853 - Psyr_0514 0.81 +2.7 570,853 - Psyr_0514 0.81 +0.1 570,853 - Psyr_0514 0.81 -0.3 570,889 + Psyr_0514 0.82 +5.1 570,889 + Psyr_0514 0.82 +0.8 570,928 + Psyr_0514 0.84 +1.4 570,928 + Psyr_0514 0.84 -2.0 571,211 + -1.0 571,211 + +0.1 571,213 + +2.6 571,213 + -1.2 571,213 + -0.6 571,213 + +3.1 571,214 - -0.9 571,214 - +1.1 571,214 - -1.1 571,214 - -0.4 571,250 + +3.1 571,250 + -1.2 571,250 + -0.8 571,250 + -1.9 571,250 + +3.5 571,251 - -0.5 571,251 - +0.8 571,251 - -0.8 571,251 - -1.6 571,251 - -0.9 571,251 - -1.0 571,323 + -1.5 571,323 - +3.3 571,324 - -1.2 571,324 - +1.1 571,324 - -0.1 571,324 - +1.7 571,327 + -0.2 571,327 + -0.9 571,327 + -2.0 571,327 + +2.5 571,327 + +3.6 571,327 + +3.5 571,327 + +3.3 571,327 + -3.6 571,327 + -0.4 571,327 + +4.3 571,328 - +0.1 571,328 - -1.9 571,328 - -0.9 571,328 - +1.3 571,328 - +0.1 571,328 - -0.5 571,328 - -1.1 571,328 - +2.1 571,328 - -2.0 571,328 - +0.3 571,328 - +1.1 571,328 - +2.5 571,328 - +2.5 571,328 - +0.7 571,348 + +3.6 571,348 + +1.1 571,348 + +3.1 571,348 + +1.7 571,349 - +0.0 571,349 - +1.1 571,349 - -0.3 571,349 - -1.5 571,349 - -0.0 571,349 - +1.3 571,349 - -0.6 571,370 + -1.5 571,398 + -0.8 571,419 + +2.1 571,419 + +3.1 571,419 + +1.1 571,419 + -0.2 571,419 + +1.7 571,419 + +3.7 571,419 + +0.2 571,419 + +4.0 571,419 + +3.8 571,419 + -2.7 571,420 - +1.7 571,420 - -3.9 571,420 - +0.2 571,420 - -1.8 571,420 - +0.1 571,434 + +2.9 571,434 + -0.0 571,435 - +2.7 571,671 + Psyr_0515 0.22 +4.3 571,671 + Psyr_0515 0.22 -0.5 571,672 - Psyr_0515 0.22 +2.5 571,672 - Psyr_0515 0.22 +0.4 571,672 - Psyr_0515 0.22 +0.6 572,424 + Psyr_0515 0.86 +2.9 572,424 + Psyr_0515 0.86 +3.7 572,424 + Psyr_0515 0.86 +2.7 572,424 + Psyr_0515 0.86 +1.8 572,424 + Psyr_0515 0.86 +0.3 572,424 + Psyr_0515 0.86 +2.3 572,424 + Psyr_0515 0.86 +3.6 572,424 + Psyr_0515 0.86 +1.3 572,425 - Psyr_0515 0.86 +0.9 572,425 - Psyr_0515 0.86 -0.7 572,428 + Psyr_0515 0.86 +1.7 572,428 + Psyr_0515 0.86 +4.2 572,428 + Psyr_0515 0.86 -0.5 572,428 + Psyr_0515 0.86 -2.9 572,428 + Psyr_0515 0.86 -1.0 572,428 + Psyr_0515 0.86 -1.5 572,429 - Psyr_0515 0.86 -0.6 572,429 - Psyr_0515 0.86 +3.6 572,429 - Psyr_0515 0.86 -0.5 572,429 - Psyr_0515 0.86 -2.3 572,429 - Psyr_0515 0.86 -1.1 572,429 - Psyr_0515 0.86 -2.8 572,429 - Psyr_0515 0.86 -1.7 572,429 - Psyr_0515 0.86 +3.1 572,429 - Psyr_0515 0.86 -0.5 572,570 + -0.9 572,570 + +0.0 572,570 + -1.6 572,571 - +2.5 572,571 - +1.9 572,571 - -1.9 572,644 + -1.1 572,644 + -2.1 572,645 - +0.5 572,645 - -2.3 572,645 - -1.5 572,645 - -0.0 572,715 + +0.2 572,715 + -0.2 572,715 + -0.7 572,715 + +2.7 572,715 + +1.7 572,715 + -1.2 572,715 + -0.5 572,715 + +3.1 572,715 + -1.2 572,715 + +3.8 572,715 + +4.1 572,716 - -0.3 572,716 - -0.0 572,716 - -0.9 572,716 - +0.3 572,716 - +0.5 572,716 - +5.3 572,716 - -0.2 572,716 - +1.1 572,716 - +2.5 572,716 - -2.7 572,716 - -0.1 572,716 - +0.9 572,716 - -1.4 572,716 - +2.1 572,716 - +0.1 572,716 - -0.9 572,716 - -0.6 572,716 - +1.7 572,716 - -3.0 572,717 + +2.9 572,717 + -1.6 572,718 - -2.0 572,718 - +4.3 572,718 - +2.7 572,718 - -0.2 572,718 - +2.7 572,718 - -0.9 572,718 - +1.3 572,738 + -2.6 572,768 - +3.3 572,861 - -3.5 573,269 + Psyr_0516 0.35 +0.0 573,270 - Psyr_0516 0.35 +2.7 573,295 - Psyr_0516 0.37 +0.6 573,438 + Psyr_0516 0.47 -1.0 573,439 - Psyr_0516 0.47 -1.6
Or see this region's nucleotide sequence