Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0512 and Psyr_0513 are separated by 226 nucleotides Psyr_0513 and Psyr_0514 overlap by 38 nucleotides Psyr_0514 and Psyr_0515 are separated by 137 nucleotides
Psyr_0512: Psyr_0512 - GCN5-related N-acetyltransferase, at 567,673 to 568,131
_0512
Psyr_0513: Psyr_0513 - amino acid ABC transporter substrate-binding protein, PAAT family, at 568,358 to 569,113
_0513
Psyr_0514: Psyr_0514 - Intergral membrane protein, YccS:Integral membrane protein, YccS/YhfK, at 569,076 to 571,274
_0514
Psyr_0515: Psyr_0515 - HI0933-like protein, at 571,412 to 572,590
_0515
Position (kb) 569
570
571
572 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 568.076 kb on + strand, within Psyr_0512 at 568.076 kb on + strand, within Psyr_0512 at 568.076 kb on + strand, within Psyr_0512 at 568.076 kb on + strand, within Psyr_0512 at 568.076 kb on + strand, within Psyr_0512 at 568.077 kb on - strand, within Psyr_0512 at 568.077 kb on - strand, within Psyr_0512 at 568.077 kb on - strand, within Psyr_0512 at 568.166 kb on + strand at 568.166 kb on + strand at 568.167 kb on - strand at 568.172 kb on + strand at 568.252 kb on - strand at 568.252 kb on - strand at 568.252 kb on - strand at 568.275 kb on + strand at 568.275 kb on + strand at 568.276 kb on - strand at 568.276 kb on - strand at 568.276 kb on - strand at 568.322 kb on + strand at 568.322 kb on + strand at 568.322 kb on + strand at 568.323 kb on - strand at 568.323 kb on - strand at 568.323 kb on - strand at 568.544 kb on + strand, within Psyr_0513 at 568.545 kb on - strand, within Psyr_0513 at 568.545 kb on - strand, within Psyr_0513 at 568.545 kb on - strand, within Psyr_0513 at 568.634 kb on - strand, within Psyr_0513 at 568.634 kb on - strand, within Psyr_0513 at 568.661 kb on + strand, within Psyr_0513 at 568.662 kb on - strand, within Psyr_0513 at 568.662 kb on - strand, within Psyr_0513 at 568.739 kb on + strand, within Psyr_0513 at 568.740 kb on - strand, within Psyr_0513 at 568.740 kb on - strand, within Psyr_0513 at 568.740 kb on - strand, within Psyr_0513 at 568.740 kb on - strand, within Psyr_0513 at 568.740 kb on - strand, within Psyr_0513 at 568.769 kb on + strand, within Psyr_0513 at 568.769 kb on + strand, within Psyr_0513 at 568.770 kb on - strand, within Psyr_0513 at 568.770 kb on - strand, within Psyr_0513 at 568.770 kb on - strand, within Psyr_0513 at 568.836 kb on + strand, within Psyr_0513 at 568.837 kb on - strand, within Psyr_0513 at 568.883 kb on + strand, within Psyr_0513 at 568.883 kb on + strand, within Psyr_0513 at 568.883 kb on + strand, within Psyr_0513 at 568.883 kb on + strand, within Psyr_0513 at 568.883 kb on + strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.884 kb on - strand, within Psyr_0513 at 568.887 kb on + strand, within Psyr_0513 at 568.887 kb on + strand, within Psyr_0513 at 568.888 kb on - strand, within Psyr_0513 at 568.888 kb on - strand, within Psyr_0513 at 568.888 kb on - strand, within Psyr_0513 at 568.895 kb on + strand, within Psyr_0513 at 568.944 kb on + strand, within Psyr_0513 at 568.976 kb on - strand, within Psyr_0513 at 569.045 kb on - strand at 569.063 kb on + strand at 569.064 kb on - strand at 569.064 kb on - strand at 569.064 kb on - strand at 569.369 kb on + strand, within Psyr_0514 at 569.398 kb on + strand, within Psyr_0514 at 569.398 kb on + strand, within Psyr_0514 at 569.398 kb on + strand, within Psyr_0514 at 569.399 kb on - strand, within Psyr_0514 at 569.596 kb on + strand, within Psyr_0514 at 569.596 kb on + strand, within Psyr_0514 at 569.596 kb on + strand, within Psyr_0514 at 569.596 kb on + strand, within Psyr_0514 at 569.596 kb on + strand, within Psyr_0514 at 569.597 kb on - strand, within Psyr_0514 at 569.597 kb on - strand, within Psyr_0514 at 569.708 kb on + strand, within Psyr_0514 at 569.709 kb on - strand, within Psyr_0514 at 569.709 kb on - strand, within Psyr_0514 at 569.709 kb on - strand, within Psyr_0514 at 569.803 kb on + strand, within Psyr_0514 at 569.876 kb on + strand, within Psyr_0514 at 570.352 kb on + strand, within Psyr_0514 at 570.352 kb on + strand, within Psyr_0514 at 570.352 kb on + strand, within Psyr_0514 at 570.352 kb on + strand, within Psyr_0514 at 570.353 kb on - strand, within Psyr_0514 at 570.369 kb on + strand, within Psyr_0514 at 570.369 kb on + strand, within Psyr_0514 at 570.370 kb on - strand, within Psyr_0514 at 570.478 kb on + strand, within Psyr_0514 at 570.478 kb on + strand, within Psyr_0514 at 570.478 kb on + strand, within Psyr_0514 at 570.479 kb on - strand, within Psyr_0514 at 570.484 kb on + strand, within Psyr_0514 at 570.529 kb on + strand, within Psyr_0514 at 570.529 kb on + strand, within Psyr_0514 at 570.529 kb on + strand, within Psyr_0514 at 570.529 kb on + strand, within Psyr_0514 at 570.530 kb on - strand, within Psyr_0514 at 570.530 kb on - strand, within Psyr_0514 at 570.852 kb on + strand, within Psyr_0514 at 570.853 kb on - strand, within Psyr_0514 at 570.853 kb on - strand, within Psyr_0514 at 570.853 kb on - strand, within Psyr_0514 at 570.853 kb on - strand, within Psyr_0514 at 570.889 kb on + strand, within Psyr_0514 at 570.889 kb on + strand, within Psyr_0514 at 570.928 kb on + strand, within Psyr_0514 at 570.928 kb on + strand, within Psyr_0514 at 571.211 kb on + strand at 571.211 kb on + strand at 571.213 kb on + strand at 571.213 kb on + strand at 571.213 kb on + strand at 571.213 kb on + strand at 571.214 kb on - strand at 571.214 kb on - strand at 571.214 kb on - strand at 571.214 kb on - strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.250 kb on + strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.251 kb on - strand at 571.323 kb on + strand at 571.323 kb on - strand at 571.324 kb on - strand at 571.324 kb on - strand at 571.324 kb on - strand at 571.324 kb on - strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.327 kb on + strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.328 kb on - strand at 571.348 kb on + strand at 571.348 kb on + strand at 571.348 kb on + strand at 571.348 kb on + strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.349 kb on - strand at 571.370 kb on + strand at 571.398 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.419 kb on + strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.420 kb on - strand at 571.434 kb on + strand at 571.434 kb on + strand at 571.435 kb on - strand at 571.671 kb on + strand, within Psyr_0515 at 571.671 kb on + strand, within Psyr_0515 at 571.672 kb on - strand, within Psyr_0515 at 571.672 kb on - strand, within Psyr_0515 at 571.672 kb on - strand, within Psyr_0515
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 568,076 + Psyr_0512 0.88 -1.7 568,076 + Psyr_0512 0.88 -0.2 568,076 + Psyr_0512 0.88 -0.6 568,076 + Psyr_0512 0.88 +1.2 568,076 + Psyr_0512 0.88 -2.2 568,077 - Psyr_0512 0.88 -2.0 568,077 - Psyr_0512 0.88 -1.7 568,077 - Psyr_0512 0.88 -3.1 568,166 + -0.6 568,166 + -0.2 568,167 - +3.7 568,172 + -1.8 568,252 - +1.7 568,252 - +0.8 568,252 - +5.1 568,275 + -0.5 568,275 + +0.7 568,276 - +0.2 568,276 - +3.7 568,276 - -0.4 568,322 + +4.5 568,322 + +3.3 568,322 + +3.9 568,323 - +0.1 568,323 - -0.9 568,323 - +2.1 568,544 + Psyr_0513 0.25 -1.2 568,545 - Psyr_0513 0.25 -0.2 568,545 - Psyr_0513 0.25 -1.1 568,545 - Psyr_0513 0.25 +1.0 568,634 - Psyr_0513 0.37 +2.5 568,634 - Psyr_0513 0.37 +3.5 568,661 + Psyr_0513 0.40 +3.1 568,662 - Psyr_0513 0.40 -0.6 568,662 - Psyr_0513 0.40 -0.4 568,739 + Psyr_0513 0.50 -2.0 568,740 - Psyr_0513 0.51 +1.7 568,740 - Psyr_0513 0.51 +1.8 568,740 - Psyr_0513 0.51 -1.1 568,740 - Psyr_0513 0.51 +0.0 568,740 - Psyr_0513 0.51 -0.3 568,769 + Psyr_0513 0.54 +1.7 568,769 + Psyr_0513 0.54 -0.9 568,770 - Psyr_0513 0.54 +3.9 568,770 - Psyr_0513 0.54 -1.0 568,770 - Psyr_0513 0.54 +0.1 568,836 + Psyr_0513 0.63 +2.1 568,837 - Psyr_0513 0.63 -3.0 568,883 + Psyr_0513 0.69 +3.1 568,883 + Psyr_0513 0.69 +0.1 568,883 + Psyr_0513 0.69 -0.4 568,883 + Psyr_0513 0.69 -1.3 568,883 + Psyr_0513 0.69 +4.2 568,884 - Psyr_0513 0.70 +3.3 568,884 - Psyr_0513 0.70 +1.1 568,884 - Psyr_0513 0.70 +3.1 568,884 - Psyr_0513 0.70 -0.1 568,884 - Psyr_0513 0.70 -0.2 568,884 - Psyr_0513 0.70 -2.3 568,884 - Psyr_0513 0.70 +0.5 568,884 - Psyr_0513 0.70 -2.6 568,884 - Psyr_0513 0.70 -0.2 568,884 - Psyr_0513 0.70 +2.1 568,884 - Psyr_0513 0.70 -2.5 568,887 + Psyr_0513 0.70 -1.3 568,887 + Psyr_0513 0.70 +0.5 568,888 - Psyr_0513 0.70 +3.9 568,888 - Psyr_0513 0.70 -4.0 568,888 - Psyr_0513 0.70 +4.1 568,895 + Psyr_0513 0.71 +0.0 568,944 + Psyr_0513 0.78 +0.5 568,976 - Psyr_0513 0.82 +0.1 569,045 - +4.6 569,063 + -1.2 569,064 - -1.8 569,064 - +2.5 569,064 - +3.5 569,369 + Psyr_0514 0.13 +3.5 569,398 + Psyr_0514 0.15 +4.0 569,398 + Psyr_0514 0.15 +0.3 569,398 + Psyr_0514 0.15 +2.3 569,399 - Psyr_0514 0.15 +3.5 569,596 + Psyr_0514 0.24 +0.7 569,596 + Psyr_0514 0.24 +3.1 569,596 + Psyr_0514 0.24 -0.6 569,596 + Psyr_0514 0.24 -0.1 569,596 + Psyr_0514 0.24 +0.3 569,597 - Psyr_0514 0.24 -0.4 569,597 - Psyr_0514 0.24 -1.2 569,708 + Psyr_0514 0.29 -0.8 569,709 - Psyr_0514 0.29 -3.0 569,709 - Psyr_0514 0.29 -0.3 569,709 - Psyr_0514 0.29 +1.5 569,803 + Psyr_0514 0.33 -0.0 569,876 + Psyr_0514 0.36 +0.1 570,352 + Psyr_0514 0.58 +3.5 570,352 + Psyr_0514 0.58 -1.3 570,352 + Psyr_0514 0.58 -0.1 570,352 + Psyr_0514 0.58 +2.1 570,353 - Psyr_0514 0.58 +1.3 570,369 + Psyr_0514 0.59 +2.9 570,369 + Psyr_0514 0.59 -0.2 570,370 - Psyr_0514 0.59 +3.1 570,478 + Psyr_0514 0.64 -0.1 570,478 + Psyr_0514 0.64 +0.1 570,478 + Psyr_0514 0.64 +0.4 570,479 - Psyr_0514 0.64 -1.7 570,484 + Psyr_0514 0.64 -0.2 570,529 + Psyr_0514 0.66 -1.0 570,529 + Psyr_0514 0.66 +2.9 570,529 + Psyr_0514 0.66 +0.5 570,529 + Psyr_0514 0.66 +1.3 570,530 - Psyr_0514 0.66 +4.0 570,530 - Psyr_0514 0.66 +0.4 570,852 + Psyr_0514 0.81 -0.8 570,853 - Psyr_0514 0.81 +3.5 570,853 - Psyr_0514 0.81 +2.7 570,853 - Psyr_0514 0.81 +0.1 570,853 - Psyr_0514 0.81 -0.3 570,889 + Psyr_0514 0.82 +5.1 570,889 + Psyr_0514 0.82 +0.8 570,928 + Psyr_0514 0.84 +1.4 570,928 + Psyr_0514 0.84 -2.0 571,211 + -1.0 571,211 + +0.1 571,213 + +2.6 571,213 + -1.2 571,213 + -0.6 571,213 + +3.1 571,214 - -0.9 571,214 - +1.1 571,214 - -1.1 571,214 - -0.4 571,250 + +3.1 571,250 + -1.2 571,250 + -0.8 571,250 + -1.9 571,250 + +3.5 571,251 - -0.5 571,251 - +0.8 571,251 - -0.8 571,251 - -1.6 571,251 - -0.9 571,251 - -1.0 571,323 + -1.5 571,323 - +3.3 571,324 - -1.2 571,324 - +1.1 571,324 - -0.1 571,324 - +1.7 571,327 + -0.2 571,327 + -0.9 571,327 + -2.0 571,327 + +2.5 571,327 + +3.6 571,327 + +3.5 571,327 + +3.3 571,327 + -3.6 571,327 + -0.4 571,327 + +4.3 571,328 - +0.1 571,328 - -1.9 571,328 - -0.9 571,328 - +1.3 571,328 - +0.1 571,328 - -0.5 571,328 - -1.1 571,328 - +2.1 571,328 - -2.0 571,328 - +0.3 571,328 - +1.1 571,328 - +2.5 571,328 - +2.5 571,328 - +0.7 571,348 + +3.6 571,348 + +1.1 571,348 + +3.1 571,348 + +1.7 571,349 - +0.0 571,349 - +1.1 571,349 - -0.3 571,349 - -1.5 571,349 - -0.0 571,349 - +1.3 571,349 - -0.6 571,370 + -1.5 571,398 + -0.8 571,419 + +2.1 571,419 + +3.1 571,419 + +1.1 571,419 + -0.2 571,419 + +1.7 571,419 + +3.7 571,419 + +0.2 571,419 + +4.0 571,419 + +3.8 571,419 + -2.7 571,420 - +1.7 571,420 - -3.9 571,420 - +0.2 571,420 - -1.8 571,420 - +0.1 571,434 + +2.9 571,434 + -0.0 571,435 - +2.7 571,671 + Psyr_0515 0.22 +4.3 571,671 + Psyr_0515 0.22 -0.5 571,672 - Psyr_0515 0.22 +2.5 571,672 - Psyr_0515 0.22 +0.4 571,672 - Psyr_0515 0.22 +0.6
Or see this region's nucleotide sequence