Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0479 and Psyr_0480 are separated by 250 nucleotides Psyr_0480 and Psyr_0481 are separated by 282 nucleotides
Psyr_0479: Psyr_0479 - NLP/P60, at 517,592 to 518,269
_0479
Psyr_0480: Psyr_0480 - TM2, at 518,520 to 518,924
_0480
Psyr_0481: Psyr_0481 - dihydroorotase, at 519,207 to 520,478
_0481
Position (kb) 518
519 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 517.654 kb on - strand at 517.743 kb on + strand, within Psyr_0479 at 517.743 kb on + strand, within Psyr_0479 at 517.744 kb on - strand, within Psyr_0479 at 517.896 kb on + strand, within Psyr_0479 at 517.896 kb on + strand, within Psyr_0479 at 517.896 kb on + strand, within Psyr_0479 at 517.896 kb on + strand, within Psyr_0479 at 517.896 kb on + strand, within Psyr_0479 at 517.896 kb on + strand, within Psyr_0479 at 517.896 kb on + strand, within Psyr_0479 at 517.896 kb on + strand, within Psyr_0479 at 517.897 kb on - strand, within Psyr_0479 at 517.897 kb on - strand, within Psyr_0479 at 517.897 kb on - strand, within Psyr_0479 at 517.986 kb on - strand, within Psyr_0479 at 518.167 kb on - strand, within Psyr_0479 at 518.167 kb on - strand, within Psyr_0479 at 518.227 kb on + strand at 518.227 kb on + strand at 518.227 kb on + strand at 518.227 kb on + strand at 518.227 kb on + strand at 518.227 kb on + strand at 518.227 kb on + strand at 518.227 kb on + strand at 518.227 kb on + strand at 518.227 kb on + strand at 518.227 kb on + strand at 518.228 kb on - strand at 518.228 kb on - strand at 518.228 kb on - strand at 518.228 kb on - strand at 518.228 kb on - strand at 518.228 kb on - strand at 518.228 kb on - strand at 518.228 kb on - strand at 518.228 kb on - strand at 518.231 kb on + strand at 518.231 kb on + strand at 518.231 kb on + strand at 518.231 kb on + strand at 518.231 kb on + strand at 518.303 kb on + strand at 518.303 kb on + strand at 518.303 kb on + strand at 518.303 kb on + strand at 518.303 kb on + strand at 518.303 kb on + strand at 518.303 kb on + strand at 518.304 kb on - strand at 518.304 kb on - strand at 518.306 kb on - strand at 518.449 kb on + strand at 518.449 kb on + strand at 518.449 kb on + strand at 518.460 kb on - strand at 518.485 kb on - strand at 518.604 kb on + strand, within Psyr_0480 at 518.605 kb on - strand, within Psyr_0480 at 518.605 kb on - strand, within Psyr_0480 at 518.605 kb on - strand, within Psyr_0480 at 518.605 kb on - strand, within Psyr_0480 at 518.605 kb on - strand, within Psyr_0480 at 518.605 kb on - strand, within Psyr_0480 at 518.605 kb on - strand, within Psyr_0480 at 518.742 kb on + strand, within Psyr_0480 at 518.799 kb on + strand, within Psyr_0480 at 518.799 kb on + strand, within Psyr_0480 at 518.799 kb on + strand, within Psyr_0480 at 518.800 kb on - strand, within Psyr_0480 at 518.800 kb on - strand, within Psyr_0480 at 518.800 kb on - strand, within Psyr_0480 at 518.800 kb on - strand, within Psyr_0480 at 518.800 kb on - strand, within Psyr_0480 at 518.800 kb on - strand, within Psyr_0480 at 518.800 kb on - strand, within Psyr_0480 at 518.868 kb on + strand, within Psyr_0480 at 518.869 kb on - strand, within Psyr_0480 at 518.966 kb on + strand at 518.966 kb on + strand at 518.966 kb on + strand at 518.967 kb on - strand at 518.967 kb on - strand at 518.967 kb on - strand at 518.988 kb on - strand at 519.164 kb on + strand at 519.218 kb on - strand at 519.798 kb on + strand, within Psyr_0481
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 517,654 - +0.1 517,743 + Psyr_0479 0.22 +0.6 517,743 + Psyr_0479 0.22 -1.4 517,744 - Psyr_0479 0.22 +0.1 517,896 + Psyr_0479 0.45 +1.3 517,896 + Psyr_0479 0.45 -2.1 517,896 + Psyr_0479 0.45 -1.4 517,896 + Psyr_0479 0.45 +0.1 517,896 + Psyr_0479 0.45 +1.7 517,896 + Psyr_0479 0.45 +2.5 517,896 + Psyr_0479 0.45 +0.9 517,896 + Psyr_0479 0.45 +1.7 517,897 - Psyr_0479 0.45 -1.6 517,897 - Psyr_0479 0.45 +1.7 517,897 - Psyr_0479 0.45 +3.5 517,986 - Psyr_0479 0.58 -1.3 518,167 - Psyr_0479 0.85 +2.9 518,167 - Psyr_0479 0.85 +2.9 518,227 + +0.8 518,227 + -1.5 518,227 + +4.2 518,227 + +3.1 518,227 + +2.1 518,227 + -0.4 518,227 + -1.6 518,227 + -0.9 518,227 + +2.5 518,227 + +1.7 518,227 + -1.4 518,228 - -0.2 518,228 - -1.6 518,228 - -2.0 518,228 - -0.3 518,228 - -2.3 518,228 - -1.1 518,228 - -0.5 518,228 - +3.3 518,228 - -1.2 518,231 + -2.2 518,231 + -1.5 518,231 + -0.8 518,231 + -1.9 518,231 + -1.2 518,303 + +3.1 518,303 + -1.9 518,303 + -3.6 518,303 + +2.9 518,303 + +2.5 518,303 + +0.7 518,303 + -0.9 518,304 - -3.4 518,304 - +0.0 518,306 - -3.2 518,449 + +1.7 518,449 + -0.8 518,449 + +2.5 518,460 - -1.1 518,485 - -0.0 518,604 + Psyr_0480 0.21 -1.6 518,605 - Psyr_0480 0.21 -2.0 518,605 - Psyr_0480 0.21 -0.2 518,605 - Psyr_0480 0.21 +3.9 518,605 - Psyr_0480 0.21 -3.6 518,605 - Psyr_0480 0.21 +4.3 518,605 - Psyr_0480 0.21 -1.6 518,605 - Psyr_0480 0.21 +1.7 518,742 + Psyr_0480 0.55 -1.4 518,799 + Psyr_0480 0.69 +0.4 518,799 + Psyr_0480 0.69 -0.2 518,799 + Psyr_0480 0.69 +0.1 518,800 - Psyr_0480 0.69 -1.9 518,800 - Psyr_0480 0.69 -2.8 518,800 - Psyr_0480 0.69 +4.1 518,800 - Psyr_0480 0.69 +3.1 518,800 - Psyr_0480 0.69 +0.0 518,800 - Psyr_0480 0.69 -0.8 518,800 - Psyr_0480 0.69 +3.3 518,868 + Psyr_0480 0.86 +3.6 518,869 - Psyr_0480 0.86 -1.6 518,966 + +0.7 518,966 + -2.4 518,966 + +3.8 518,967 - +4.0 518,967 - +0.1 518,967 - -0.7 518,988 - +3.5 519,164 + -0.0 519,218 - -0.9 519,798 + Psyr_0481 0.46 +0.0
Or see this region's nucleotide sequence