Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0469 and Psyr_0470 are separated by 75 nucleotides Psyr_0470 and Psyr_0471 are separated by 9 nucleotides Psyr_0471 and Psyr_0472 overlap by 4 nucleotides Psyr_0472 and Psyr_0473 are separated by 33 nucleotides
Psyr_0469: Psyr_0469 - dihydroxyacid dehydratase, at 508,137 to 509,984
_0469
Psyr_0470: Psyr_0470 - conserved hypothetical protein, at 510,060 to 510,383
_0470
Psyr_0471: Psyr_0471 - coproporphyrinogen III oxidase, anaerobic, at 510,393 to 511,607
_0471
Psyr_0472: Psyr_0472 - Ham1-like protein, at 511,604 to 512,197
_0472
Psyr_0473: Psyr_0473 - Methionine biosynthesis MetW, at 512,231 to 512,851
_0473
Position (kb)
510
511
512 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 509.404 kb on + strand, within Psyr_0469 at 509.405 kb on - strand, within Psyr_0469 at 509.405 kb on - strand, within Psyr_0469 at 509.405 kb on - strand, within Psyr_0469 at 509.405 kb on - strand, within Psyr_0469 at 509.406 kb on + strand, within Psyr_0469 at 509.406 kb on + strand, within Psyr_0469 at 509.407 kb on - strand, within Psyr_0469 at 509.611 kb on - strand, within Psyr_0469 at 509.623 kb on - strand, within Psyr_0469 at 509.910 kb on + strand at 509.996 kb on - strand at 510.002 kb on + strand at 510.003 kb on - strand at 510.003 kb on - strand at 510.003 kb on - strand at 510.061 kb on + strand at 510.061 kb on + strand at 510.061 kb on + strand at 510.062 kb on - strand at 510.062 kb on - strand at 510.062 kb on - strand at 510.124 kb on + strand, within Psyr_0470 at 510.124 kb on + strand, within Psyr_0470 at 510.124 kb on + strand, within Psyr_0470 at 510.124 kb on + strand, within Psyr_0470 at 510.169 kb on + strand, within Psyr_0470 at 510.169 kb on + strand, within Psyr_0470 at 510.170 kb on - strand, within Psyr_0470 at 510.170 kb on - strand, within Psyr_0470 at 510.394 kb on + strand at 510.394 kb on + strand at 510.394 kb on + strand at 510.394 kb on + strand at 510.394 kb on + strand at 510.394 kb on + strand at 510.395 kb on - strand at 510.395 kb on - strand at 510.406 kb on + strand at 510.406 kb on + strand at 510.407 kb on - strand at 510.550 kb on - strand, within Psyr_0471 at 510.665 kb on - strand, within Psyr_0471 at 510.692 kb on + strand, within Psyr_0471 at 510.772 kb on + strand, within Psyr_0471 at 510.772 kb on + strand, within Psyr_0471 at 510.772 kb on + strand, within Psyr_0471 at 510.772 kb on + strand, within Psyr_0471 at 510.811 kb on + strand, within Psyr_0471 at 510.811 kb on + strand, within Psyr_0471 at 510.812 kb on - strand, within Psyr_0471 at 510.826 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.417 kb on + strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.418 kb on - strand, within Psyr_0471 at 511.469 kb on - strand, within Psyr_0471 at 511.496 kb on + strand at 511.496 kb on + strand at 511.497 kb on - strand at 511.504 kb on + strand at 511.504 kb on + strand at 511.504 kb on + strand at 511.504 kb on + strand at 511.505 kb on - strand at 511.505 kb on - strand at 511.505 kb on - strand at 511.505 kb on - strand at 511.505 kb on - strand at 511.505 kb on - strand at 511.835 kb on + strand, within Psyr_0472 at 511.835 kb on + strand, within Psyr_0472 at 511.836 kb on - strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.944 kb on + strand, within Psyr_0472 at 511.945 kb on - strand, within Psyr_0472 at 511.945 kb on - strand, within Psyr_0472 at 511.945 kb on - strand, within Psyr_0472 at 511.945 kb on - strand, within Psyr_0472 at 511.945 kb on - strand, within Psyr_0472 at 511.945 kb on - strand, within Psyr_0472 at 511.948 kb on + strand, within Psyr_0472 at 512.277 kb on + strand at 512.277 kb on + strand at 512.303 kb on - strand, within Psyr_0473 at 512.436 kb on + strand, within Psyr_0473 at 512.437 kb on - strand, within Psyr_0473 at 512.443 kb on + strand, within Psyr_0473 at 512.444 kb on - strand, within Psyr_0473 at 512.444 kb on - strand, within Psyr_0473 at 512.445 kb on + strand, within Psyr_0473 at 512.445 kb on + strand, within Psyr_0473 at 512.445 kb on + strand, within Psyr_0473 at 512.446 kb on - strand, within Psyr_0473 at 512.446 kb on - strand, within Psyr_0473 at 512.446 kb on - strand, within Psyr_0473 at 512.490 kb on + strand, within Psyr_0473 at 512.490 kb on + strand, within Psyr_0473 at 512.491 kb on - strand, within Psyr_0473 at 512.491 kb on - strand, within Psyr_0473
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 509,404 + Psyr_0469 0.69 -1.0 509,405 - Psyr_0469 0.69 +4.4 509,405 - Psyr_0469 0.69 -0.2 509,405 - Psyr_0469 0.69 -1.0 509,405 - Psyr_0469 0.69 +0.1 509,406 + Psyr_0469 0.69 +2.5 509,406 + Psyr_0469 0.69 -0.9 509,407 - Psyr_0469 0.69 +3.1 509,611 - Psyr_0469 0.80 +0.1 509,623 - Psyr_0469 0.80 +0.4 509,910 + +2.1 509,996 - -0.3 510,002 + +2.9 510,003 - +0.7 510,003 - -2.0 510,003 - -1.8 510,061 + +3.3 510,061 + -0.5 510,061 + +3.1 510,062 - -1.4 510,062 - +0.0 510,062 - -0.4 510,124 + Psyr_0470 0.20 -0.7 510,124 + Psyr_0470 0.20 -0.9 510,124 + Psyr_0470 0.20 +0.5 510,124 + Psyr_0470 0.20 -0.9 510,169 + Psyr_0470 0.34 +0.2 510,169 + Psyr_0470 0.34 -1.2 510,170 - Psyr_0470 0.34 -0.8 510,170 - Psyr_0470 0.34 +3.3 510,394 + -0.5 510,394 + +3.5 510,394 + +1.5 510,394 + +3.0 510,394 + +0.8 510,394 + -0.9 510,395 - +2.9 510,395 - +2.7 510,406 + +2.7 510,406 + +1.7 510,407 - +3.8 510,550 - Psyr_0471 0.13 -0.0 510,665 - Psyr_0471 0.22 +0.1 510,692 + Psyr_0471 0.25 +1.7 510,772 + Psyr_0471 0.31 +1.7 510,772 + Psyr_0471 0.31 -2.0 510,772 + Psyr_0471 0.31 -1.8 510,772 + Psyr_0471 0.31 +2.5 510,811 + Psyr_0471 0.34 +0.1 510,811 + Psyr_0471 0.34 -2.5 510,812 - Psyr_0471 0.34 +1.7 510,826 + Psyr_0471 0.36 -2.0 511,417 + Psyr_0471 0.84 -2.7 511,417 + Psyr_0471 0.84 +3.9 511,417 + Psyr_0471 0.84 -1.1 511,417 + Psyr_0471 0.84 -0.7 511,417 + Psyr_0471 0.84 +1.1 511,417 + Psyr_0471 0.84 -1.3 511,417 + Psyr_0471 0.84 +0.7 511,417 + Psyr_0471 0.84 +0.2 511,417 + Psyr_0471 0.84 -1.4 511,417 + Psyr_0471 0.84 +1.2 511,417 + Psyr_0471 0.84 +2.5 511,418 - Psyr_0471 0.84 -0.9 511,418 - Psyr_0471 0.84 -1.8 511,418 - Psyr_0471 0.84 +3.3 511,418 - Psyr_0471 0.84 -0.4 511,418 - Psyr_0471 0.84 -2.3 511,418 - Psyr_0471 0.84 -1.2 511,418 - Psyr_0471 0.84 -0.4 511,469 - Psyr_0471 0.89 -0.7 511,496 + -1.0 511,496 + +0.7 511,497 - +0.7 511,504 + -0.9 511,504 + +1.1 511,504 + +3.9 511,504 + +0.1 511,505 - +2.5 511,505 - +0.8 511,505 - -1.3 511,505 - -1.0 511,505 - +2.7 511,505 - -0.1 511,835 + Psyr_0472 0.39 +0.6 511,835 + Psyr_0472 0.39 -0.8 511,836 - Psyr_0472 0.39 +0.1 511,944 + Psyr_0472 0.57 +2.7 511,944 + Psyr_0472 0.57 +3.6 511,944 + Psyr_0472 0.57 -2.3 511,944 + Psyr_0472 0.57 -2.8 511,944 + Psyr_0472 0.57 +1.7 511,944 + Psyr_0472 0.57 -0.0 511,944 + Psyr_0472 0.57 -2.4 511,944 + Psyr_0472 0.57 -1.1 511,944 + Psyr_0472 0.57 +0.7 511,944 + Psyr_0472 0.57 -0.7 511,945 - Psyr_0472 0.57 -0.0 511,945 - Psyr_0472 0.57 -1.3 511,945 - Psyr_0472 0.57 -0.3 511,945 - Psyr_0472 0.57 +2.7 511,945 - Psyr_0472 0.57 +1.7 511,945 - Psyr_0472 0.57 +0.7 511,948 + Psyr_0472 0.58 +1.1 512,277 + -0.9 512,277 + -1.4 512,303 - Psyr_0473 0.12 +4.3 512,436 + Psyr_0473 0.33 +2.1 512,437 - Psyr_0473 0.33 -1.4 512,443 + Psyr_0473 0.34 -1.6 512,444 - Psyr_0473 0.34 -2.4 512,444 - Psyr_0473 0.34 -0.7 512,445 + Psyr_0473 0.34 -1.4 512,445 + Psyr_0473 0.34 -0.8 512,445 + Psyr_0473 0.34 -1.9 512,446 - Psyr_0473 0.35 -3.7 512,446 - Psyr_0473 0.35 +2.5 512,446 - Psyr_0473 0.35 -0.6 512,490 + Psyr_0473 0.42 +2.1 512,490 + Psyr_0473 0.42 +1.8 512,491 - Psyr_0473 0.42 +2.5 512,491 - Psyr_0473 0.42 +1.3
Or see this region's nucleotide sequence