Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0429 and Psyr_0430 overlap by 29 nucleotides Psyr_0430 and Psyr_0431 overlap by 4 nucleotides Psyr_0431 and Psyr_0432 are separated by 54 nucleotides
Psyr_0429: Psyr_0429 - membrane protein, putative, at 467,655 to 470,030
_0429
Psyr_0430: Psyr_0430 - conserved hypothetical protein, at 470,002 to 470,616
_0430
Psyr_0431: Psyr_0431 - Thioesterase superfamily, at 470,613 to 471,062
_0431
Psyr_0432: Psyr_0432 - conserved hypothetical protein, at 471,117 to 472,064
_0432
Psyr_0433: Psyr_0433 - Glycosyl transferase, family 2, at 472,061 to 472,795
_0433
Position (kb) 470
471
472 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 469.959 kb on + strand at 469.960 kb on - strand at 470.632 kb on - strand at 470.632 kb on - strand at 470.729 kb on + strand, within Psyr_0431 at 470.730 kb on - strand, within Psyr_0431 at 470.858 kb on - strand, within Psyr_0431 at 470.858 kb on - strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.918 kb on + strand, within Psyr_0431 at 470.918 kb on + strand, within Psyr_0431 at 470.919 kb on - strand, within Psyr_0431 at 470.919 kb on - strand, within Psyr_0431 at 470.919 kb on - strand, within Psyr_0431 at 470.962 kb on + strand, within Psyr_0431 at 470.962 kb on + strand, within Psyr_0431 at 470.962 kb on + strand, within Psyr_0431 at 470.971 kb on + strand, within Psyr_0431 at 470.971 kb on + strand, within Psyr_0431 at 470.972 kb on - strand, within Psyr_0431 at 470.972 kb on - strand, within Psyr_0431 at 470.972 kb on - strand, within Psyr_0431 at 471.064 kb on + strand at 471.064 kb on + strand at 471.064 kb on + strand at 471.064 kb on + strand at 471.064 kb on + strand at 471.065 kb on - strand at 471.065 kb on - strand at 471.065 kb on - strand at 471.093 kb on + strand at 471.093 kb on + strand at 471.093 kb on + strand at 471.094 kb on - strand at 471.094 kb on - strand at 471.094 kb on - strand at 471.094 kb on - strand at 471.094 kb on - strand at 471.878 kb on - strand, within Psyr_0432 at 471.923 kb on - strand, within Psyr_0432 at 471.923 kb on - strand, within Psyr_0432 at 471.923 kb on - strand, within Psyr_0432 at 471.937 kb on + strand, within Psyr_0432
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 469,959 + +2.1 469,960 - -1.4 470,632 - +0.6 470,632 - +2.1 470,729 + Psyr_0431 0.26 +0.6 470,730 - Psyr_0431 0.26 +0.1 470,858 - Psyr_0431 0.54 +0.8 470,858 - Psyr_0431 0.54 +3.5 470,914 + Psyr_0431 0.67 -0.5 470,914 + Psyr_0431 0.67 +0.1 470,914 + Psyr_0431 0.67 -5.1 470,914 + Psyr_0431 0.67 -0.5 470,914 + Psyr_0431 0.67 -2.4 470,914 + Psyr_0431 0.67 -1.2 470,914 + Psyr_0431 0.67 -1.3 470,914 + Psyr_0431 0.67 +4.4 470,914 + Psyr_0431 0.67 +3.8 470,914 + Psyr_0431 0.67 +4.4 470,914 + Psyr_0431 0.67 +2.5 470,914 + Psyr_0431 0.67 -0.6 470,914 + Psyr_0431 0.67 -0.9 470,914 + Psyr_0431 0.67 +3.1 470,914 + Psyr_0431 0.67 +2.7 470,914 + Psyr_0431 0.67 +3.5 470,914 + Psyr_0431 0.67 -0.3 470,914 + Psyr_0431 0.67 -2.2 470,914 + Psyr_0431 0.67 -0.4 470,915 - Psyr_0431 0.67 +0.4 470,915 - Psyr_0431 0.67 +1.1 470,915 - Psyr_0431 0.67 -0.7 470,915 - Psyr_0431 0.67 -0.6 470,915 - Psyr_0431 0.67 +1.6 470,915 - Psyr_0431 0.67 -2.4 470,915 - Psyr_0431 0.67 +0.6 470,915 - Psyr_0431 0.67 -2.2 470,915 - Psyr_0431 0.67 -2.5 470,915 - Psyr_0431 0.67 +2.5 470,918 + Psyr_0431 0.68 -3.2 470,918 + Psyr_0431 0.68 -1.0 470,919 - Psyr_0431 0.68 +2.5 470,919 - Psyr_0431 0.68 +3.5 470,919 - Psyr_0431 0.68 +4.3 470,962 + Psyr_0431 0.78 +3.7 470,962 + Psyr_0431 0.78 -0.4 470,962 + Psyr_0431 0.78 +3.6 470,971 + Psyr_0431 0.80 +1.1 470,971 + Psyr_0431 0.80 -0.6 470,972 - Psyr_0431 0.80 -1.1 470,972 - Psyr_0431 0.80 +0.9 470,972 - Psyr_0431 0.80 +1.7 471,064 + -1.2 471,064 + +0.1 471,064 + +0.3 471,064 + +0.6 471,064 + -2.1 471,065 - -0.9 471,065 - -0.4 471,065 - +2.7 471,093 + -1.6 471,093 + +1.8 471,093 + +0.3 471,094 - +1.1 471,094 - +1.7 471,094 - -1.7 471,094 - -1.2 471,094 - -0.4 471,878 - Psyr_0432 0.80 -0.7 471,923 - Psyr_0432 0.85 +0.9 471,923 - Psyr_0432 0.85 -0.5 471,923 - Psyr_0432 0.85 -1.2 471,937 + Psyr_0432 0.86 +4.0
Or see this region's nucleotide sequence