Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0428 and Psyr_0429 are separated by 54 nucleotides Psyr_0429 and Psyr_0430 overlap by 29 nucleotides Psyr_0430 and Psyr_0431 overlap by 4 nucleotides
Psyr_0428: Psyr_0428 - Monooxygenase, FAD-binding protein, at 466,353 to 467,600
_0428
Psyr_0429: Psyr_0429 - membrane protein, putative, at 467,655 to 470,030
_0429
Psyr_0430: Psyr_0430 - conserved hypothetical protein, at 470,002 to 470,616
_0430
Psyr_0431: Psyr_0431 - Thioesterase superfamily, at 470,613 to 471,062
_0431
Position (kb) 467
468
469
470
471 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 467.140 kb on + strand, within Psyr_0428 at 467.141 kb on - strand, within Psyr_0428 at 467.342 kb on - strand, within Psyr_0428 at 467.342 kb on - strand, within Psyr_0428 at 467.342 kb on - strand, within Psyr_0428 at 467.342 kb on - strand, within Psyr_0428 at 467.342 kb on - strand, within Psyr_0428 at 467.342 kb on - strand, within Psyr_0428 at 467.604 kb on + strand at 467.605 kb on - strand at 467.699 kb on + strand at 467.869 kb on - strand at 467.894 kb on - strand, within Psyr_0429 at 468.066 kb on + strand, within Psyr_0429 at 468.066 kb on + strand, within Psyr_0429 at 468.339 kb on + strand, within Psyr_0429 at 468.339 kb on + strand, within Psyr_0429 at 468.404 kb on - strand, within Psyr_0429 at 468.481 kb on + strand, within Psyr_0429 at 468.481 kb on + strand, within Psyr_0429 at 468.482 kb on - strand, within Psyr_0429 at 468.482 kb on - strand, within Psyr_0429 at 468.482 kb on - strand, within Psyr_0429 at 468.482 kb on - strand, within Psyr_0429 at 468.482 kb on - strand, within Psyr_0429 at 468.482 kb on - strand, within Psyr_0429 at 468.482 kb on - strand, within Psyr_0429 at 468.482 kb on - strand, within Psyr_0429 at 468.869 kb on - strand, within Psyr_0429 at 469.048 kb on + strand, within Psyr_0429 at 469.048 kb on + strand, within Psyr_0429 at 469.049 kb on - strand, within Psyr_0429 at 469.049 kb on - strand, within Psyr_0429 at 469.049 kb on - strand, within Psyr_0429 at 469.049 kb on - strand, within Psyr_0429 at 469.298 kb on - strand, within Psyr_0429 at 469.298 kb on - strand, within Psyr_0429 at 469.443 kb on - strand, within Psyr_0429 at 469.597 kb on - strand, within Psyr_0429 at 469.959 kb on + strand at 469.960 kb on - strand at 470.632 kb on - strand at 470.632 kb on - strand at 470.729 kb on + strand, within Psyr_0431 at 470.730 kb on - strand, within Psyr_0431 at 470.858 kb on - strand, within Psyr_0431 at 470.858 kb on - strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.914 kb on + strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.915 kb on - strand, within Psyr_0431 at 470.918 kb on + strand, within Psyr_0431 at 470.918 kb on + strand, within Psyr_0431 at 470.919 kb on - strand, within Psyr_0431 at 470.919 kb on - strand, within Psyr_0431 at 470.919 kb on - strand, within Psyr_0431 at 470.962 kb on + strand, within Psyr_0431 at 470.962 kb on + strand, within Psyr_0431 at 470.962 kb on + strand, within Psyr_0431 at 470.971 kb on + strand, within Psyr_0431 at 470.971 kb on + strand, within Psyr_0431 at 470.972 kb on - strand, within Psyr_0431 at 470.972 kb on - strand, within Psyr_0431 at 470.972 kb on - strand, within Psyr_0431
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 467,140 + Psyr_0428 0.63 -0.5 467,141 - Psyr_0428 0.63 -1.9 467,342 - Psyr_0428 0.79 +1.7 467,342 - Psyr_0428 0.79 +1.7 467,342 - Psyr_0428 0.79 -0.9 467,342 - Psyr_0428 0.79 -2.2 467,342 - Psyr_0428 0.79 +1.2 467,342 - Psyr_0428 0.79 -0.9 467,604 + +2.7 467,605 - +0.5 467,699 + +3.8 467,869 - -2.2 467,894 - Psyr_0429 0.10 -2.0 468,066 + Psyr_0429 0.17 -2.0 468,066 + Psyr_0429 0.17 +2.0 468,339 + Psyr_0429 0.29 +2.7 468,339 + Psyr_0429 0.29 +1.7 468,404 - Psyr_0429 0.32 +2.1 468,481 + Psyr_0429 0.35 +4.4 468,481 + Psyr_0429 0.35 +0.3 468,482 - Psyr_0429 0.35 -0.7 468,482 - Psyr_0429 0.35 -1.0 468,482 - Psyr_0429 0.35 +3.9 468,482 - Psyr_0429 0.35 +2.6 468,482 - Psyr_0429 0.35 -0.9 468,482 - Psyr_0429 0.35 +2.5 468,482 - Psyr_0429 0.35 -0.1 468,482 - Psyr_0429 0.35 +4.1 468,869 - Psyr_0429 0.51 -1.7 469,048 + Psyr_0429 0.59 +0.3 469,048 + Psyr_0429 0.59 -1.5 469,049 - Psyr_0429 0.59 -0.8 469,049 - Psyr_0429 0.59 +0.7 469,049 - Psyr_0429 0.59 +0.0 469,049 - Psyr_0429 0.59 +1.7 469,298 - Psyr_0429 0.69 +1.1 469,298 - Psyr_0429 0.69 +2.5 469,443 - Psyr_0429 0.75 +3.7 469,597 - Psyr_0429 0.82 +4.1 469,959 + +2.1 469,960 - -1.4 470,632 - +0.6 470,632 - +2.1 470,729 + Psyr_0431 0.26 +0.6 470,730 - Psyr_0431 0.26 +0.1 470,858 - Psyr_0431 0.54 +0.8 470,858 - Psyr_0431 0.54 +3.5 470,914 + Psyr_0431 0.67 -0.5 470,914 + Psyr_0431 0.67 +0.1 470,914 + Psyr_0431 0.67 -5.1 470,914 + Psyr_0431 0.67 -0.5 470,914 + Psyr_0431 0.67 -2.4 470,914 + Psyr_0431 0.67 -1.2 470,914 + Psyr_0431 0.67 -1.3 470,914 + Psyr_0431 0.67 +4.4 470,914 + Psyr_0431 0.67 +3.8 470,914 + Psyr_0431 0.67 +4.4 470,914 + Psyr_0431 0.67 +2.5 470,914 + Psyr_0431 0.67 -0.6 470,914 + Psyr_0431 0.67 -0.9 470,914 + Psyr_0431 0.67 +3.1 470,914 + Psyr_0431 0.67 +2.7 470,914 + Psyr_0431 0.67 +3.5 470,914 + Psyr_0431 0.67 -0.3 470,914 + Psyr_0431 0.67 -2.2 470,914 + Psyr_0431 0.67 -0.4 470,915 - Psyr_0431 0.67 +0.4 470,915 - Psyr_0431 0.67 +1.1 470,915 - Psyr_0431 0.67 -0.7 470,915 - Psyr_0431 0.67 -0.6 470,915 - Psyr_0431 0.67 +1.6 470,915 - Psyr_0431 0.67 -2.4 470,915 - Psyr_0431 0.67 +0.6 470,915 - Psyr_0431 0.67 -2.2 470,915 - Psyr_0431 0.67 -2.5 470,915 - Psyr_0431 0.67 +2.5 470,918 + Psyr_0431 0.68 -3.2 470,918 + Psyr_0431 0.68 -1.0 470,919 - Psyr_0431 0.68 +2.5 470,919 - Psyr_0431 0.68 +3.5 470,919 - Psyr_0431 0.68 +4.3 470,962 + Psyr_0431 0.78 +3.7 470,962 + Psyr_0431 0.78 -0.4 470,962 + Psyr_0431 0.78 +3.6 470,971 + Psyr_0431 0.80 +1.1 470,971 + Psyr_0431 0.80 -0.6 470,972 - Psyr_0431 0.80 -1.1 470,972 - Psyr_0431 0.80 +0.9 470,972 - Psyr_0431 0.80 +1.7
Or see this region's nucleotide sequence