Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0377 and Psyr_0378 overlap by 8 nucleotides
Psyr_0377: Psyr_0377 - Periplasmic glucan biosynthesis protein, MdoG, at 402,055 to 403,983
_0377
Psyr_0378: Psyr_0378 - Glycosyl transferase, family 2, at 403,976 to 406,558
_0378
Position (kb) 403
404
405
406
407 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 402.981 kb on + strand, within Psyr_0377 at 403.063 kb on + strand, within Psyr_0377 at 403.063 kb on + strand, within Psyr_0377 at 403.190 kb on + strand, within Psyr_0377 at 403.190 kb on + strand, within Psyr_0377 at 403.190 kb on + strand, within Psyr_0377 at 403.191 kb on - strand, within Psyr_0377 at 403.191 kb on - strand, within Psyr_0377 at 403.191 kb on - strand, within Psyr_0377 at 403.195 kb on + strand, within Psyr_0377 at 403.571 kb on - strand, within Psyr_0377 at 403.706 kb on + strand, within Psyr_0377 at 403.706 kb on + strand, within Psyr_0377 at 403.960 kb on + strand at 403.961 kb on - strand at 403.961 kb on - strand at 403.961 kb on - strand at 403.981 kb on + strand at 403.981 kb on + strand at 403.981 kb on + strand at 403.981 kb on + strand at 403.982 kb on - strand at 403.982 kb on - strand at 403.982 kb on - strand at 403.982 kb on - strand at 403.982 kb on - strand at 403.982 kb on - strand at 404.016 kb on - strand at 404.028 kb on + strand at 404.408 kb on + strand, within Psyr_0378 at 404.409 kb on - strand, within Psyr_0378 at 404.409 kb on - strand, within Psyr_0378 at 404.456 kb on + strand, within Psyr_0378 at 404.456 kb on + strand, within Psyr_0378 at 404.457 kb on - strand, within Psyr_0378 at 404.457 kb on - strand, within Psyr_0378 at 404.457 kb on - strand, within Psyr_0378 at 404.555 kb on + strand, within Psyr_0378 at 404.556 kb on - strand, within Psyr_0378 at 404.670 kb on - strand, within Psyr_0378 at 404.670 kb on - strand, within Psyr_0378 at 404.722 kb on + strand, within Psyr_0378 at 404.722 kb on + strand, within Psyr_0378 at 404.722 kb on + strand, within Psyr_0378 at 404.722 kb on + strand, within Psyr_0378 at 404.722 kb on + strand, within Psyr_0378 at 404.723 kb on - strand, within Psyr_0378 at 404.785 kb on + strand, within Psyr_0378 at 404.786 kb on - strand, within Psyr_0378 at 404.955 kb on + strand, within Psyr_0378 at 404.955 kb on + strand, within Psyr_0378 at 405.014 kb on + strand, within Psyr_0378 at 405.014 kb on + strand, within Psyr_0378 at 405.015 kb on - strand, within Psyr_0378 at 405.015 kb on - strand, within Psyr_0378 at 405.015 kb on - strand, within Psyr_0378 at 405.015 kb on - strand, within Psyr_0378 at 405.015 kb on - strand, within Psyr_0378 at 405.015 kb on - strand, within Psyr_0378 at 405.015 kb on - strand, within Psyr_0378 at 405.146 kb on + strand, within Psyr_0378 at 405.146 kb on + strand, within Psyr_0378 at 405.146 kb on + strand, within Psyr_0378 at 405.147 kb on - strand, within Psyr_0378 at 405.147 kb on - strand, within Psyr_0378 at 405.179 kb on + strand, within Psyr_0378 at 405.180 kb on - strand, within Psyr_0378 at 405.180 kb on - strand, within Psyr_0378 at 405.180 kb on - strand, within Psyr_0378 at 405.180 kb on - strand, within Psyr_0378 at 405.180 kb on - strand, within Psyr_0378 at 405.410 kb on + strand, within Psyr_0378 at 405.411 kb on - strand, within Psyr_0378 at 405.554 kb on + strand, within Psyr_0378 at 405.554 kb on + strand, within Psyr_0378 at 405.555 kb on - strand, within Psyr_0378 at 405.555 kb on - strand, within Psyr_0378 at 405.555 kb on - strand, within Psyr_0378 at 405.635 kb on + strand, within Psyr_0378 at 405.636 kb on - strand, within Psyr_0378 at 406.193 kb on + strand, within Psyr_0378 at 406.199 kb on + strand, within Psyr_0378 at 406.199 kb on + strand, within Psyr_0378 at 406.199 kb on + strand, within Psyr_0378 at 406.200 kb on - strand, within Psyr_0378 at 406.200 kb on - strand, within Psyr_0378 at 406.200 kb on - strand, within Psyr_0378 at 406.200 kb on - strand, within Psyr_0378 at 406.200 kb on - strand, within Psyr_0378 at 406.327 kb on + strand at 406.531 kb on + strand at 406.532 kb on - strand at 406.556 kb on + strand at 406.717 kb on - strand at 407.120 kb on + strand at 407.120 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 402,981 + Psyr_0377 0.48 -1.5 403,063 + Psyr_0377 0.52 -1.0 403,063 + Psyr_0377 0.52 +1.1 403,190 + Psyr_0377 0.59 +1.7 403,190 + Psyr_0377 0.59 +3.1 403,190 + Psyr_0377 0.59 +2.5 403,191 - Psyr_0377 0.59 -1.8 403,191 - Psyr_0377 0.59 -2.7 403,191 - Psyr_0377 0.59 -0.8 403,195 + Psyr_0377 0.59 +2.7 403,571 - Psyr_0377 0.79 -1.0 403,706 + Psyr_0377 0.86 +2.5 403,706 + Psyr_0377 0.86 -4.7 403,960 + -1.9 403,961 - -5.8 403,961 - -2.9 403,961 - -1.3 403,981 + +2.5 403,981 + +3.6 403,981 + -1.6 403,981 + -1.6 403,982 - -1.7 403,982 - -1.2 403,982 - -3.8 403,982 - +2.9 403,982 - +3.5 403,982 - +3.5 404,016 - +0.1 404,028 + -0.8 404,408 + Psyr_0378 0.17 -2.2 404,409 - Psyr_0378 0.17 -1.0 404,409 - Psyr_0378 0.17 -2.8 404,456 + Psyr_0378 0.19 +2.1 404,456 + Psyr_0378 0.19 -2.7 404,457 - Psyr_0378 0.19 +0.1 404,457 - Psyr_0378 0.19 +0.1 404,457 - Psyr_0378 0.19 -0.5 404,555 + Psyr_0378 0.22 -2.4 404,556 - Psyr_0378 0.22 -1.7 404,670 - Psyr_0378 0.27 +1.7 404,670 - Psyr_0378 0.27 -3.0 404,722 + Psyr_0378 0.29 +0.1 404,722 + Psyr_0378 0.29 +1.7 404,722 + Psyr_0378 0.29 +0.1 404,722 + Psyr_0378 0.29 +1.7 404,722 + Psyr_0378 0.29 -1.0 404,723 - Psyr_0378 0.29 -2.5 404,785 + Psyr_0378 0.31 -2.1 404,786 - Psyr_0378 0.31 -4.3 404,955 + Psyr_0378 0.38 -1.3 404,955 + Psyr_0378 0.38 +0.1 405,014 + Psyr_0378 0.40 -1.2 405,014 + Psyr_0378 0.40 -1.6 405,015 - Psyr_0378 0.40 -1.0 405,015 - Psyr_0378 0.40 +2.9 405,015 - Psyr_0378 0.40 +1.7 405,015 - Psyr_0378 0.40 -2.1 405,015 - Psyr_0378 0.40 +0.1 405,015 - Psyr_0378 0.40 +3.7 405,015 - Psyr_0378 0.40 +0.1 405,146 + Psyr_0378 0.45 -2.7 405,146 + Psyr_0378 0.45 -3.0 405,146 + Psyr_0378 0.45 +0.1 405,147 - Psyr_0378 0.45 -1.5 405,147 - Psyr_0378 0.45 +1.1 405,179 + Psyr_0378 0.47 -2.5 405,180 - Psyr_0378 0.47 +1.1 405,180 - Psyr_0378 0.47 -2.2 405,180 - Psyr_0378 0.47 -1.5 405,180 - Psyr_0378 0.47 +1.1 405,180 - Psyr_0378 0.47 -1.7 405,410 + Psyr_0378 0.56 +1.1 405,411 - Psyr_0378 0.56 -2.7 405,554 + Psyr_0378 0.61 +1.7 405,554 + Psyr_0378 0.61 -3.4 405,555 - Psyr_0378 0.61 -2.5 405,555 - Psyr_0378 0.61 +0.1 405,555 - Psyr_0378 0.61 -0.7 405,635 + Psyr_0378 0.64 +2.1 405,636 - Psyr_0378 0.64 +2.5 406,193 + Psyr_0378 0.86 -0.9 406,199 + Psyr_0378 0.86 -3.3 406,199 + Psyr_0378 0.86 +0.1 406,199 + Psyr_0378 0.86 +1.7 406,200 - Psyr_0378 0.86 -2.0 406,200 - Psyr_0378 0.86 -0.2 406,200 - Psyr_0378 0.86 +0.1 406,200 - Psyr_0378 0.86 +2.1 406,200 - Psyr_0378 0.86 +1.7 406,327 + -0.6 406,531 + -0.4 406,532 - -0.2 406,556 + +0.5 406,717 - -1.2 407,120 + +2.5 407,120 + +4.8
Or see this region's nucleotide sequence