Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0375 and Psyr_0376 are separated by 39 nucleotides Psyr_0376 and Psyr_0377 are separated by 263 nucleotides Psyr_0377 and Psyr_0378 overlap by 8 nucleotides
Psyr_0375: Psyr_0375 - prolyl aminopeptidase, Serine peptidase, MEROPS family S33, at 400,343 to 401,314
_0375
Psyr_0376: Psyr_0376 - D-tyrosyl-tRNA(Tyr) deacylase, at 401,354 to 401,791
_0376
Psyr_0377: Psyr_0377 - Periplasmic glucan biosynthesis protein, MdoG, at 402,055 to 403,983
_0377
Psyr_0378: Psyr_0378 - Glycosyl transferase, family 2, at 403,976 to 406,558
_0378
Position (kb) 402
403
404 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 401.133 kb on - strand, within Psyr_0375 at 401.133 kb on - strand, within Psyr_0375 at 401.353 kb on + strand at 401.353 kb on + strand at 401.353 kb on + strand at 401.353 kb on + strand at 401.353 kb on + strand at 401.353 kb on + strand at 401.354 kb on - strand at 401.354 kb on - strand at 401.354 kb on - strand at 401.354 kb on - strand at 401.354 kb on - strand at 401.354 kb on - strand at 401.354 kb on - strand at 401.433 kb on + strand, within Psyr_0376 at 401.433 kb on + strand, within Psyr_0376 at 401.434 kb on - strand, within Psyr_0376 at 401.451 kb on + strand, within Psyr_0376 at 401.452 kb on - strand, within Psyr_0376 at 401.483 kb on - strand, within Psyr_0376 at 401.675 kb on + strand, within Psyr_0376 at 401.712 kb on + strand, within Psyr_0376 at 401.754 kb on + strand at 401.785 kb on + strand at 401.785 kb on + strand at 401.785 kb on + strand at 401.785 kb on + strand at 401.785 kb on + strand at 401.785 kb on + strand at 401.785 kb on + strand at 401.786 kb on - strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.789 kb on + strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.790 kb on - strand at 401.794 kb on + strand at 401.794 kb on + strand at 401.795 kb on - strand at 401.807 kb on + strand at 401.807 kb on + strand at 401.807 kb on + strand at 401.807 kb on + strand at 401.807 kb on + strand at 401.808 kb on - strand at 401.808 kb on - strand at 401.853 kb on + strand at 401.853 kb on + strand at 401.853 kb on + strand at 401.853 kb on + strand at 401.853 kb on + strand at 401.854 kb on - strand at 401.854 kb on - strand at 401.965 kb on - strand at 402.106 kb on - strand at 402.106 kb on - strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.134 kb on + strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.135 kb on - strand at 402.183 kb on - strand at 402.346 kb on + strand, within Psyr_0377 at 402.347 kb on - strand, within Psyr_0377 at 402.347 kb on - strand, within Psyr_0377 at 402.421 kb on + strand, within Psyr_0377 at 402.421 kb on + strand, within Psyr_0377 at 402.421 kb on + strand, within Psyr_0377 at 402.422 kb on - strand, within Psyr_0377 at 402.422 kb on - strand, within Psyr_0377 at 402.565 kb on + strand, within Psyr_0377 at 402.596 kb on - strand, within Psyr_0377 at 402.596 kb on - strand, within Psyr_0377 at 402.596 kb on - strand, within Psyr_0377 at 402.855 kb on + strand, within Psyr_0377 at 402.855 kb on + strand, within Psyr_0377 at 402.855 kb on + strand, within Psyr_0377 at 402.855 kb on + strand, within Psyr_0377 at 402.855 kb on + strand, within Psyr_0377 at 402.855 kb on + strand, within Psyr_0377 at 402.855 kb on + strand, within Psyr_0377 at 402.855 kb on + strand, within Psyr_0377 at 402.856 kb on - strand, within Psyr_0377 at 402.856 kb on - strand, within Psyr_0377 at 402.856 kb on - strand, within Psyr_0377 at 402.856 kb on - strand, within Psyr_0377 at 402.856 kb on - strand, within Psyr_0377 at 402.895 kb on - strand, within Psyr_0377 at 402.895 kb on - strand, within Psyr_0377 at 402.898 kb on + strand, within Psyr_0377 at 402.899 kb on - strand, within Psyr_0377 at 402.981 kb on + strand, within Psyr_0377 at 403.063 kb on + strand, within Psyr_0377 at 403.063 kb on + strand, within Psyr_0377 at 403.190 kb on + strand, within Psyr_0377 at 403.190 kb on + strand, within Psyr_0377 at 403.190 kb on + strand, within Psyr_0377 at 403.191 kb on - strand, within Psyr_0377 at 403.191 kb on - strand, within Psyr_0377 at 403.191 kb on - strand, within Psyr_0377 at 403.195 kb on + strand, within Psyr_0377 at 403.571 kb on - strand, within Psyr_0377 at 403.706 kb on + strand, within Psyr_0377 at 403.706 kb on + strand, within Psyr_0377 at 403.960 kb on + strand at 403.961 kb on - strand at 403.961 kb on - strand at 403.961 kb on - strand at 403.981 kb on + strand at 403.981 kb on + strand at 403.981 kb on + strand at 403.981 kb on + strand at 403.982 kb on - strand at 403.982 kb on - strand at 403.982 kb on - strand at 403.982 kb on - strand at 403.982 kb on - strand at 403.982 kb on - strand at 404.016 kb on - strand at 404.028 kb on + strand at 404.408 kb on + strand, within Psyr_0378 at 404.409 kb on - strand, within Psyr_0378 at 404.409 kb on - strand, within Psyr_0378 at 404.456 kb on + strand, within Psyr_0378 at 404.456 kb on + strand, within Psyr_0378 at 404.457 kb on - strand, within Psyr_0378 at 404.457 kb on - strand, within Psyr_0378 at 404.457 kb on - strand, within Psyr_0378 at 404.555 kb on + strand, within Psyr_0378 at 404.556 kb on - strand, within Psyr_0378 at 404.670 kb on - strand, within Psyr_0378 at 404.670 kb on - strand, within Psyr_0378 at 404.722 kb on + strand, within Psyr_0378 at 404.722 kb on + strand, within Psyr_0378 at 404.722 kb on + strand, within Psyr_0378 at 404.722 kb on + strand, within Psyr_0378 at 404.722 kb on + strand, within Psyr_0378 at 404.723 kb on - strand, within Psyr_0378 at 404.785 kb on + strand, within Psyr_0378 at 404.786 kb on - strand, within Psyr_0378 at 404.955 kb on + strand, within Psyr_0378 at 404.955 kb on + strand, within Psyr_0378
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 401,133 - Psyr_0375 0.81 -1.1 401,133 - Psyr_0375 0.81 +2.6 401,353 + -0.1 401,353 + -3.6 401,353 + +2.1 401,353 + +4.2 401,353 + -1.8 401,353 + -0.4 401,354 - -1.9 401,354 - -2.8 401,354 - +2.5 401,354 - +2.5 401,354 - -0.0 401,354 - +0.1 401,354 - -1.2 401,433 + Psyr_0376 0.18 +1.1 401,433 + Psyr_0376 0.18 -0.5 401,434 - Psyr_0376 0.18 +0.1 401,451 + Psyr_0376 0.22 +3.9 401,452 - Psyr_0376 0.22 -2.5 401,483 - Psyr_0376 0.29 +1.1 401,675 + Psyr_0376 0.73 -2.4 401,712 + Psyr_0376 0.82 -0.4 401,754 + -0.6 401,785 + +1.7 401,785 + +5.0 401,785 + +0.6 401,785 + -5.0 401,785 + -2.3 401,785 + -2.6 401,785 + +0.7 401,786 - +0.1 401,789 + +3.1 401,789 + +0.8 401,789 + -0.5 401,789 + +3.7 401,789 + +3.6 401,789 + +2.5 401,789 + -3.5 401,789 + -1.3 401,789 + -4.3 401,789 + -0.8 401,789 + -2.8 401,789 + -2.8 401,789 + -0.3 401,789 + +2.7 401,790 - -1.0 401,790 - -2.8 401,790 - -0.6 401,790 - -1.1 401,790 - +2.1 401,790 - -0.9 401,790 - -0.1 401,790 - -2.5 401,790 - +2.5 401,790 - -1.0 401,790 - -0.6 401,790 - +0.1 401,794 + -0.3 401,794 + -1.7 401,795 - -2.9 401,807 + -0.0 401,807 + +2.1 401,807 + -1.1 401,807 + -0.4 401,807 + -1.5 401,808 - +1.7 401,808 - -3.1 401,853 + -1.3 401,853 + -0.4 401,853 + -1.2 401,853 + +3.3 401,853 + +2.5 401,854 - -1.2 401,854 - +1.7 401,965 - -0.9 402,106 - -2.0 402,106 - -1.5 402,134 + -1.8 402,134 + -2.9 402,134 + -1.0 402,134 + -1.8 402,134 + -1.6 402,134 + +0.1 402,134 + +2.7 402,134 + -1.4 402,134 + -0.0 402,134 + +0.1 402,134 + -2.7 402,134 + -1.2 402,134 + +0.1 402,134 + +1.7 402,134 + +1.1 402,134 + +1.1 402,134 + +1.7 402,134 + +3.6 402,134 + +1.1 402,135 - -2.1 402,135 - +1.1 402,135 - -1.7 402,135 - -2.9 402,135 - +1.7 402,135 - +2.1 402,135 - +1.1 402,135 - +1.1 402,135 - +3.1 402,135 - +2.1 402,135 - +2.9 402,135 - +3.6 402,135 - -1.6 402,135 - -0.8 402,135 - +0.1 402,135 - -3.4 402,135 - +0.1 402,135 - -0.9 402,135 - -1.1 402,135 - +2.7 402,183 - -1.0 402,346 + Psyr_0377 0.15 +0.1 402,347 - Psyr_0377 0.15 -1.7 402,347 - Psyr_0377 0.15 +1.1 402,421 + Psyr_0377 0.19 -2.3 402,421 + Psyr_0377 0.19 -1.3 402,421 + Psyr_0377 0.19 -2.5 402,422 - Psyr_0377 0.19 -1.1 402,422 - Psyr_0377 0.19 -2.8 402,565 + Psyr_0377 0.26 -0.6 402,596 - Psyr_0377 0.28 -2.0 402,596 - Psyr_0377 0.28 +2.5 402,596 - Psyr_0377 0.28 -3.8 402,855 + Psyr_0377 0.41 +3.1 402,855 + Psyr_0377 0.41 -3.8 402,855 + Psyr_0377 0.41 -2.0 402,855 + Psyr_0377 0.41 +1.1 402,855 + Psyr_0377 0.41 -1.4 402,855 + Psyr_0377 0.41 -1.5 402,855 + Psyr_0377 0.41 -3.0 402,855 + Psyr_0377 0.41 +0.1 402,856 - Psyr_0377 0.42 -3.5 402,856 - Psyr_0377 0.42 +1.0 402,856 - Psyr_0377 0.42 -2.9 402,856 - Psyr_0377 0.42 +2.1 402,856 - Psyr_0377 0.42 -3.8 402,895 - Psyr_0377 0.44 +0.1 402,895 - Psyr_0377 0.44 +2.5 402,898 + Psyr_0377 0.44 +3.1 402,899 - Psyr_0377 0.44 -3.0 402,981 + Psyr_0377 0.48 -1.5 403,063 + Psyr_0377 0.52 -1.0 403,063 + Psyr_0377 0.52 +1.1 403,190 + Psyr_0377 0.59 +1.7 403,190 + Psyr_0377 0.59 +3.1 403,190 + Psyr_0377 0.59 +2.5 403,191 - Psyr_0377 0.59 -1.8 403,191 - Psyr_0377 0.59 -2.7 403,191 - Psyr_0377 0.59 -0.8 403,195 + Psyr_0377 0.59 +2.7 403,571 - Psyr_0377 0.79 -1.0 403,706 + Psyr_0377 0.86 +2.5 403,706 + Psyr_0377 0.86 -4.7 403,960 + -1.9 403,961 - -5.8 403,961 - -2.9 403,961 - -1.3 403,981 + +2.5 403,981 + +3.6 403,981 + -1.6 403,981 + -1.6 403,982 - -1.7 403,982 - -1.2 403,982 - -3.8 403,982 - +2.9 403,982 - +3.5 403,982 - +3.5 404,016 - +0.1 404,028 + -0.8 404,408 + Psyr_0378 0.17 -2.2 404,409 - Psyr_0378 0.17 -1.0 404,409 - Psyr_0378 0.17 -2.8 404,456 + Psyr_0378 0.19 +2.1 404,456 + Psyr_0378 0.19 -2.7 404,457 - Psyr_0378 0.19 +0.1 404,457 - Psyr_0378 0.19 +0.1 404,457 - Psyr_0378 0.19 -0.5 404,555 + Psyr_0378 0.22 -2.4 404,556 - Psyr_0378 0.22 -1.7 404,670 - Psyr_0378 0.27 +1.7 404,670 - Psyr_0378 0.27 -3.0 404,722 + Psyr_0378 0.29 +0.1 404,722 + Psyr_0378 0.29 +1.7 404,722 + Psyr_0378 0.29 +0.1 404,722 + Psyr_0378 0.29 +1.7 404,722 + Psyr_0378 0.29 -1.0 404,723 - Psyr_0378 0.29 -2.5 404,785 + Psyr_0378 0.31 -2.1 404,786 - Psyr_0378 0.31 -4.3 404,955 + Psyr_0378 0.38 -1.3 404,955 + Psyr_0378 0.38 +0.1
Or see this region's nucleotide sequence