Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0363

Experiment: KB with Acridine orange 62.5 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0361 and Psyr_0362 overlap by 4 nucleotidesPsyr_0362 and Psyr_0363 are separated by 103 nucleotidesPsyr_0363 and Psyr_0364 are separated by 105 nucleotides Psyr_0361: Psyr_0361 - conserved hypothetical protein, at 386,578 to 387,285 _0361 Psyr_0362: Psyr_0362 - Sigma-70 region 2, at 387,282 to 387,848 _0362 Psyr_0363: Psyr_0363 - conserved hypothetical protein, at 387,952 to 388,485 _0363 Psyr_0364: Psyr_0364 - sodium/proton antiporter, NhaA family, at 388,591 to 389,766 _0364 Position (kb) 387 388 389Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 387.069 kb on + strand, within Psyr_0361at 387.199 kb on + strand, within Psyr_0361at 387.199 kb on + strand, within Psyr_0361at 387.200 kb on - strand, within Psyr_0361at 387.200 kb on - strand, within Psyr_0361at 387.200 kb on - strand, within Psyr_0361at 387.200 kb on - strand, within Psyr_0361at 387.200 kb on - strand, within Psyr_0361at 387.235 kb on + strandat 387.235 kb on + strandat 387.235 kb on + strandat 387.235 kb on + strandat 387.235 kb on + strandat 387.235 kb on + strandat 387.236 kb on - strandat 387.236 kb on - strandat 387.236 kb on - strandat 387.236 kb on - strandat 387.236 kb on - strandat 387.236 kb on - strandat 387.236 kb on - strandat 387.239 kb on + strandat 387.239 kb on + strandat 387.239 kb on + strandat 387.239 kb on + strandat 387.239 kb on + strandat 387.240 kb on - strandat 387.240 kb on - strandat 387.240 kb on - strandat 387.272 kb on - strandat 387.272 kb on - strandat 387.272 kb on - strandat 387.390 kb on + strand, within Psyr_0362at 387.390 kb on + strand, within Psyr_0362at 387.390 kb on + strand, within Psyr_0362at 387.391 kb on - strand, within Psyr_0362at 387.391 kb on - strand, within Psyr_0362at 387.391 kb on - strand, within Psyr_0362at 387.391 kb on - strand, within Psyr_0362at 387.391 kb on - strand, within Psyr_0362at 387.394 kb on + strand, within Psyr_0362at 387.394 kb on + strand, within Psyr_0362at 387.394 kb on + strand, within Psyr_0362at 387.395 kb on - strand, within Psyr_0362at 387.395 kb on - strand, within Psyr_0362at 387.395 kb on - strand, within Psyr_0362at 387.395 kb on - strand, within Psyr_0362at 387.395 kb on - strand, within Psyr_0362at 387.395 kb on - strand, within Psyr_0362at 387.407 kb on + strand, within Psyr_0362at 387.407 kb on + strand, within Psyr_0362at 387.407 kb on + strand, within Psyr_0362at 387.407 kb on + strand, within Psyr_0362at 387.408 kb on - strand, within Psyr_0362at 387.873 kb on + strandat 387.873 kb on + strandat 387.874 kb on - strandat 387.874 kb on - strandat 387.914 kb on - strandat 387.914 kb on - strandat 387.918 kb on + strandat 387.918 kb on + strandat 387.918 kb on + strandat 387.919 kb on - strandat 387.919 kb on - strandat 387.919 kb on - strandat 387.919 kb on - strandat 387.919 kb on - strandat 387.919 kb on - strandat 387.919 kb on - strandat 387.940 kb on + strandat 387.975 kb on - strandat 387.986 kb on + strandat 387.986 kb on + strandat 387.987 kb on - strandat 388.073 kb on + strand, within Psyr_0363at 388.073 kb on + strand, within Psyr_0363at 388.074 kb on - strand, within Psyr_0363at 388.074 kb on - strand, within Psyr_0363at 388.074 kb on - strand, within Psyr_0363at 388.074 kb on - strand, within Psyr_0363at 388.074 kb on - strand, within Psyr_0363at 388.130 kb on + strand, within Psyr_0363at 388.210 kb on - strand, within Psyr_0363at 388.223 kb on + strand, within Psyr_0363at 388.224 kb on - strand, within Psyr_0363at 388.226 kb on + strand, within Psyr_0363at 388.226 kb on + strand, within Psyr_0363at 388.226 kb on + strand, within Psyr_0363at 388.226 kb on + strand, within Psyr_0363at 388.227 kb on - strand, within Psyr_0363at 388.227 kb on - strand, within Psyr_0363at 388.227 kb on - strand, within Psyr_0363at 388.227 kb on - strand, within Psyr_0363at 388.227 kb on - strand, within Psyr_0363at 388.227 kb on - strand, within Psyr_0363at 388.328 kb on + strand, within Psyr_0363at 388.328 kb on + strand, within Psyr_0363at 388.328 kb on + strand, within Psyr_0363at 388.329 kb on - strand, within Psyr_0363at 388.329 kb on - strand, within Psyr_0363at 388.329 kb on - strand, within Psyr_0363at 388.329 kb on - strand, within Psyr_0363at 388.329 kb on - strand, within Psyr_0363at 388.453 kb on - strandat 388.542 kb on + strandat 388.542 kb on + strandat 388.542 kb on + strandat 388.542 kb on + strandat 388.543 kb on - strandat 388.543 kb on - strandat 388.543 kb on - strandat 388.543 kb on - strandat 388.543 kb on - strandat 388.543 kb on - strandat 388.543 kb on - strandat 388.543 kb on - strandat 388.545 kb on + strandat 388.585 kb on - strandat 388.661 kb on - strandat 388.766 kb on + strand, within Psyr_0364at 388.914 kb on + strand, within Psyr_0364at 388.915 kb on - strand, within Psyr_0364at 388.915 kb on - strand, within Psyr_0364at 388.915 kb on - strand, within Psyr_0364at 388.982 kb on + strand, within Psyr_0364at 388.983 kb on - strand, within Psyr_0364at 388.983 kb on - strand, within Psyr_0364at 389.136 kb on - strand, within Psyr_0364at 389.136 kb on - strand, within Psyr_0364at 389.136 kb on - strand, within Psyr_0364at 389.234 kb on + strand, within Psyr_0364at 389.234 kb on + strand, within Psyr_0364at 389.234 kb on + strand, within Psyr_0364at 389.235 kb on - strand, within Psyr_0364at 389.423 kb on + strand, within Psyr_0364at 389.423 kb on + strand, within Psyr_0364at 389.423 kb on + strand, within Psyr_0364at 389.423 kb on + strand, within Psyr_0364at 389.424 kb on - strand, within Psyr_0364

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml
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387,069 + Psyr_0361 0.69 +1.1
387,199 + Psyr_0361 0.88 -1.6
387,199 + Psyr_0361 0.88 +3.3
387,200 - Psyr_0361 0.88 +1.1
387,200 - Psyr_0361 0.88 +3.3
387,200 - Psyr_0361 0.88 -2.3
387,200 - Psyr_0361 0.88 -1.7
387,200 - Psyr_0361 0.88 +4.4
387,235 + -0.6
387,235 + -0.9
387,235 + -0.2
387,235 + +0.1
387,235 + +1.1
387,235 + -2.9
387,236 - +0.6
387,236 - -0.7
387,236 - -1.3
387,236 - -2.1
387,236 - +0.9
387,236 - -3.7
387,236 - +2.3
387,239 + -0.2
387,239 + +2.5
387,239 + -2.0
387,239 + +1.7
387,239 + +1.5
387,240 - -0.1
387,240 - -0.4
387,240 - +1.3
387,272 - -0.9
387,272 - -1.3
387,272 - -0.1
387,390 + Psyr_0362 0.19 +1.1
387,390 + Psyr_0362 0.19 -0.9
387,390 + Psyr_0362 0.19 -1.4
387,391 - Psyr_0362 0.19 -0.4
387,391 - Psyr_0362 0.19 -2.0
387,391 - Psyr_0362 0.19 +3.1
387,391 - Psyr_0362 0.19 -0.8
387,391 - Psyr_0362 0.19 -2.4
387,394 + Psyr_0362 0.20 +0.1
387,394 + Psyr_0362 0.20 +0.1
387,394 + Psyr_0362 0.20 +0.1
387,395 - Psyr_0362 0.20 +1.7
387,395 - Psyr_0362 0.20 +2.7
387,395 - Psyr_0362 0.20 -1.3
387,395 - Psyr_0362 0.20 -0.7
387,395 - Psyr_0362 0.20 +3.8
387,395 - Psyr_0362 0.20 +0.1
387,407 + Psyr_0362 0.22 +1.1
387,407 + Psyr_0362 0.22 +0.9
387,407 + Psyr_0362 0.22 +1.3
387,407 + Psyr_0362 0.22 +1.3
387,408 - Psyr_0362 0.22 -1.2
387,873 + -3.6
387,873 + +0.7
387,874 - -1.2
387,874 - -0.3
387,914 - +0.1
387,914 - -1.0
387,918 + +3.5
387,918 + -3.4
387,918 + +0.1
387,919 - +0.9
387,919 - -1.1
387,919 - +3.3
387,919 - +3.7
387,919 - +3.9
387,919 - -2.9
387,919 - +0.1
387,940 + +0.6
387,975 - -0.1
387,986 + -0.7
387,986 + -0.6
387,987 - -0.3
388,073 + Psyr_0363 0.23 +0.8
388,073 + Psyr_0363 0.23 +2.7
388,074 - Psyr_0363 0.23 +0.1
388,074 - Psyr_0363 0.23 +1.7
388,074 - Psyr_0363 0.23 -2.1
388,074 - Psyr_0363 0.23 +2.5
388,074 - Psyr_0363 0.23 +4.1
388,130 + Psyr_0363 0.33 -1.4
388,210 - Psyr_0363 0.48 +0.3
388,223 + Psyr_0363 0.51 +3.8
388,224 - Psyr_0363 0.51 -2.2
388,226 + Psyr_0363 0.51 +3.8
388,226 + Psyr_0363 0.51 +1.6
388,226 + Psyr_0363 0.51 -0.4
388,226 + Psyr_0363 0.51 -3.8
388,227 - Psyr_0363 0.51 +4.4
388,227 - Psyr_0363 0.51 -1.3
388,227 - Psyr_0363 0.51 -1.3
388,227 - Psyr_0363 0.51 +0.9
388,227 - Psyr_0363 0.51 -1.4
388,227 - Psyr_0363 0.51 +2.9
388,328 + Psyr_0363 0.70 -2.1
388,328 + Psyr_0363 0.70 -0.9
388,328 + Psyr_0363 0.70 +2.1
388,329 - Psyr_0363 0.71 +1.1
388,329 - Psyr_0363 0.71 +3.3
388,329 - Psyr_0363 0.71 +0.1
388,329 - Psyr_0363 0.71 +1.5
388,329 - Psyr_0363 0.71 -0.3
388,453 - -1.5
388,542 + -1.4
388,542 + -1.9
388,542 + +2.7
388,542 + +4.4
388,543 - -2.4
388,543 - +0.1
388,543 - -2.8
388,543 - -0.0
388,543 - +2.7
388,543 - -0.5
388,543 - +1.0
388,543 - -0.9
388,545 + -2.5
388,585 - -1.5
388,661 - +2.1
388,766 + Psyr_0364 0.15 +3.1
388,914 + Psyr_0364 0.27 +1.5
388,915 - Psyr_0364 0.28 -0.9
388,915 - Psyr_0364 0.28 +2.9
388,915 - Psyr_0364 0.28 -0.4
388,982 + Psyr_0364 0.33 -3.9
388,983 - Psyr_0364 0.33 +2.5
388,983 - Psyr_0364 0.33 -0.1
389,136 - Psyr_0364 0.46 +1.1
389,136 - Psyr_0364 0.46 +1.7
389,136 - Psyr_0364 0.46 +2.7
389,234 + Psyr_0364 0.55 +1.1
389,234 + Psyr_0364 0.55 +2.9
389,234 + Psyr_0364 0.55 +0.1
389,235 - Psyr_0364 0.55 -1.9
389,423 + Psyr_0364 0.71 -1.6
389,423 + Psyr_0364 0.71 +0.3
389,423 + Psyr_0364 0.71 -0.4
389,423 + Psyr_0364 0.71 -2.7
389,424 - Psyr_0364 0.71 +1.1

Or see this region's nucleotide sequence