Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0340 and Psyr_0341 are separated by 625 nucleotides Psyr_0341 and Psyr_0342 are separated by 89 nucleotides
Psyr_0340: Psyr_0340 - ABC transporter, periplasmic substrate-binding protein, at 362,237 to 363,256
_0340
Psyr_0341: Psyr_0341 - hypothetical protein, at 363,882 to 364,115
_0341
Psyr_0342: Psyr_0342 - hypothetical protein, at 364,205 to 365,200
_0342
Position (kb) 363
364
365 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 362.887 kb on + strand, within Psyr_0340 at 362.887 kb on + strand, within Psyr_0340 at 362.887 kb on + strand, within Psyr_0340 at 362.887 kb on + strand, within Psyr_0340 at 362.887 kb on + strand, within Psyr_0340 at 362.887 kb on + strand, within Psyr_0340 at 362.888 kb on - strand, within Psyr_0340 at 362.888 kb on - strand, within Psyr_0340 at 362.888 kb on - strand, within Psyr_0340 at 362.903 kb on + strand, within Psyr_0340 at 362.903 kb on + strand, within Psyr_0340 at 362.903 kb on + strand, within Psyr_0340 at 362.904 kb on - strand, within Psyr_0340 at 362.904 kb on - strand, within Psyr_0340 at 362.942 kb on + strand, within Psyr_0340 at 362.942 kb on + strand, within Psyr_0340 at 362.942 kb on + strand, within Psyr_0340 at 362.943 kb on - strand, within Psyr_0340 at 362.943 kb on - strand, within Psyr_0340 at 362.943 kb on - strand, within Psyr_0340 at 362.943 kb on - strand, within Psyr_0340 at 362.943 kb on - strand, within Psyr_0340 at 362.943 kb on - strand, within Psyr_0340 at 362.981 kb on + strand, within Psyr_0340 at 362.981 kb on + strand, within Psyr_0340 at 362.981 kb on + strand, within Psyr_0340 at 362.982 kb on - strand, within Psyr_0340 at 362.982 kb on - strand, within Psyr_0340 at 362.993 kb on + strand, within Psyr_0340 at 362.993 kb on + strand, within Psyr_0340 at 362.993 kb on + strand, within Psyr_0340 at 362.994 kb on - strand, within Psyr_0340 at 362.994 kb on - strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.056 kb on + strand, within Psyr_0340 at 363.057 kb on - strand, within Psyr_0340 at 363.057 kb on - strand, within Psyr_0340 at 363.057 kb on - strand, within Psyr_0340 at 363.057 kb on - strand, within Psyr_0340 at 363.057 kb on - strand, within Psyr_0340 at 363.159 kb on - strand at 363.159 kb on - strand at 363.323 kb on + strand at 363.323 kb on + strand at 363.323 kb on + strand at 363.323 kb on + strand at 363.324 kb on - strand at 363.324 kb on - strand at 363.348 kb on - strand at 363.401 kb on + strand at 363.401 kb on + strand at 363.401 kb on + strand at 363.401 kb on + strand at 363.402 kb on - strand at 363.452 kb on + strand at 363.453 kb on - strand at 363.453 kb on - strand at 363.453 kb on - strand at 363.453 kb on - strand at 363.453 kb on - strand at 363.453 kb on - strand at 363.453 kb on - strand at 363.544 kb on - strand at 363.606 kb on + strand at 363.606 kb on + strand at 363.607 kb on - strand at 363.658 kb on + strand at 363.658 kb on + strand at 363.658 kb on + strand at 363.658 kb on + strand at 363.659 kb on - strand at 363.659 kb on - strand at 363.659 kb on - strand at 363.659 kb on - strand at 363.659 kb on - strand at 363.839 kb on - strand at 363.869 kb on - strand at 363.869 kb on - strand at 363.876 kb on + strand at 363.877 kb on - strand at 363.952 kb on + strand, within Psyr_0341 at 363.952 kb on + strand, within Psyr_0341 at 363.952 kb on + strand, within Psyr_0341 at 363.952 kb on + strand, within Psyr_0341 at 363.953 kb on - strand, within Psyr_0341 at 363.953 kb on - strand, within Psyr_0341 at 363.953 kb on - strand, within Psyr_0341 at 363.953 kb on - strand, within Psyr_0341 at 363.953 kb on - strand, within Psyr_0341 at 363.979 kb on - strand, within Psyr_0341 at 364.113 kb on + strand at 364.113 kb on + strand at 364.122 kb on + strand at 364.122 kb on + strand at 364.122 kb on + strand at 364.122 kb on + strand at 364.301 kb on + strand at 364.301 kb on + strand at 364.301 kb on + strand at 364.301 kb on + strand at 364.381 kb on + strand, within Psyr_0342 at 364.556 kb on + strand, within Psyr_0342 at 364.556 kb on + strand, within Psyr_0342 at 364.557 kb on - strand, within Psyr_0342 at 364.583 kb on + strand, within Psyr_0342 at 364.583 kb on + strand, within Psyr_0342 at 364.584 kb on - strand, within Psyr_0342 at 364.655 kb on + strand, within Psyr_0342 at 364.662 kb on - strand, within Psyr_0342 at 364.662 kb on - strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.730 kb on + strand, within Psyr_0342 at 364.731 kb on - strand, within Psyr_0342 at 364.731 kb on - strand, within Psyr_0342 at 364.731 kb on - strand, within Psyr_0342 at 364.731 kb on - strand, within Psyr_0342 at 364.731 kb on - strand, within Psyr_0342 at 364.731 kb on - strand, within Psyr_0342 at 364.731 kb on - strand, within Psyr_0342 at 364.731 kb on - strand, within Psyr_0342 at 364.731 kb on - strand, within Psyr_0342 at 364.731 kb on - strand, within Psyr_0342 at 364.736 kb on + strand, within Psyr_0342 at 364.736 kb on + strand, within Psyr_0342 at 364.736 kb on + strand, within Psyr_0342 at 364.736 kb on + strand, within Psyr_0342 at 364.736 kb on + strand, within Psyr_0342 at 364.736 kb on + strand, within Psyr_0342 at 364.737 kb on - strand, within Psyr_0342 at 364.737 kb on - strand, within Psyr_0342 at 364.737 kb on - strand, within Psyr_0342 at 364.737 kb on - strand, within Psyr_0342 at 364.737 kb on - strand, within Psyr_0342 at 364.793 kb on - strand, within Psyr_0342 at 364.793 kb on - strand, within Psyr_0342 at 364.958 kb on + strand, within Psyr_0342 at 364.958 kb on + strand, within Psyr_0342 at 364.958 kb on + strand, within Psyr_0342 at 364.958 kb on + strand, within Psyr_0342 at 364.958 kb on + strand, within Psyr_0342 at 364.958 kb on + strand, within Psyr_0342 at 364.959 kb on - strand, within Psyr_0342 at 364.959 kb on - strand, within Psyr_0342 at 364.959 kb on - strand, within Psyr_0342 at 364.959 kb on - strand, within Psyr_0342 at 364.959 kb on - strand, within Psyr_0342 at 364.959 kb on - strand, within Psyr_0342 at 364.959 kb on - strand, within Psyr_0342 at 364.959 kb on - strand, within Psyr_0342 at 364.959 kb on - strand, within Psyr_0342 at 364.959 kb on - strand, within Psyr_0342 at 364.960 kb on + strand, within Psyr_0342 at 364.960 kb on + strand, within Psyr_0342 at 364.960 kb on + strand, within Psyr_0342 at 364.960 kb on + strand, within Psyr_0342 at 364.960 kb on + strand, within Psyr_0342 at 364.960 kb on + strand, within Psyr_0342 at 364.961 kb on - strand, within Psyr_0342 at 364.961 kb on - strand, within Psyr_0342 at 364.961 kb on - strand, within Psyr_0342 at 364.961 kb on - strand, within Psyr_0342 at 364.961 kb on - strand, within Psyr_0342 at 364.961 kb on - strand, within Psyr_0342 at 364.970 kb on - strand, within Psyr_0342 at 364.999 kb on + strand, within Psyr_0342 at 364.999 kb on + strand, within Psyr_0342 at 365.000 kb on - strand, within Psyr_0342 at 365.017 kb on + strand, within Psyr_0342 at 365.017 kb on + strand, within Psyr_0342 at 365.017 kb on + strand, within Psyr_0342 at 365.018 kb on - strand, within Psyr_0342 at 365.018 kb on - strand, within Psyr_0342 at 365.019 kb on + strand, within Psyr_0342 at 365.019 kb on + strand, within Psyr_0342 at 365.019 kb on + strand, within Psyr_0342 at 365.019 kb on + strand, within Psyr_0342 at 365.019 kb on + strand, within Psyr_0342 at 365.019 kb on + strand, within Psyr_0342 at 365.019 kb on + strand, within Psyr_0342 at 365.019 kb on + strand, within Psyr_0342 at 365.019 kb on + strand, within Psyr_0342 at 365.019 kb on + strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.020 kb on - strand, within Psyr_0342 at 365.060 kb on + strand, within Psyr_0342 at 365.060 kb on + strand, within Psyr_0342 at 365.060 kb on + strand, within Psyr_0342 at 365.060 kb on + strand, within Psyr_0342 at 365.060 kb on + strand, within Psyr_0342 at 365.060 kb on + strand, within Psyr_0342 at 365.060 kb on + strand, within Psyr_0342 at 365.060 kb on + strand, within Psyr_0342 at 365.061 kb on - strand, within Psyr_0342 at 365.061 kb on - strand, within Psyr_0342 at 365.061 kb on - strand, within Psyr_0342 at 365.061 kb on - strand, within Psyr_0342 at 365.061 kb on - strand, within Psyr_0342 at 365.061 kb on - strand, within Psyr_0342 at 365.063 kb on + strand, within Psyr_0342 at 365.063 kb on + strand, within Psyr_0342 at 365.064 kb on - strand, within Psyr_0342
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 362,887 + Psyr_0340 0.64 +3.5 362,887 + Psyr_0340 0.64 -1.9 362,887 + Psyr_0340 0.64 +2.9 362,887 + Psyr_0340 0.64 +0.2 362,887 + Psyr_0340 0.64 +1.5 362,887 + Psyr_0340 0.64 -1.9 362,888 - Psyr_0340 0.64 -0.1 362,888 - Psyr_0340 0.64 -1.6 362,888 - Psyr_0340 0.64 +4.2 362,903 + Psyr_0340 0.65 +3.3 362,903 + Psyr_0340 0.65 -1.6 362,903 + Psyr_0340 0.65 +0.0 362,904 - Psyr_0340 0.65 -3.1 362,904 - Psyr_0340 0.65 +4.5 362,942 + Psyr_0340 0.69 -1.0 362,942 + Psyr_0340 0.69 +1.7 362,942 + Psyr_0340 0.69 +0.3 362,943 - Psyr_0340 0.69 -2.3 362,943 - Psyr_0340 0.69 +0.5 362,943 - Psyr_0340 0.69 -1.6 362,943 - Psyr_0340 0.69 -1.6 362,943 - Psyr_0340 0.69 -1.4 362,943 - Psyr_0340 0.69 +2.7 362,981 + Psyr_0340 0.73 +2.1 362,981 + Psyr_0340 0.73 +4.5 362,981 + Psyr_0340 0.73 +0.3 362,982 - Psyr_0340 0.73 +0.2 362,982 - Psyr_0340 0.73 +2.1 362,993 + Psyr_0340 0.74 -1.9 362,993 + Psyr_0340 0.74 +0.3 362,993 + Psyr_0340 0.74 +2.0 362,994 - Psyr_0340 0.74 -1.2 362,994 - Psyr_0340 0.74 +2.9 363,056 + Psyr_0340 0.80 -0.1 363,056 + Psyr_0340 0.80 +1.1 363,056 + Psyr_0340 0.80 -0.6 363,056 + Psyr_0340 0.80 +2.5 363,056 + Psyr_0340 0.80 -1.6 363,056 + Psyr_0340 0.80 -1.2 363,056 + Psyr_0340 0.80 +2.1 363,057 - Psyr_0340 0.80 -1.0 363,057 - Psyr_0340 0.80 +0.0 363,057 - Psyr_0340 0.80 -2.2 363,057 - Psyr_0340 0.80 -0.6 363,057 - Psyr_0340 0.80 -1.9 363,159 - +2.1 363,159 - +4.4 363,323 + -2.2 363,323 + +2.7 363,323 + +2.9 363,323 + +2.5 363,324 - -1.1 363,324 - -0.1 363,348 - -0.6 363,401 + +3.3 363,401 + -0.1 363,401 + +0.6 363,401 + -0.7 363,402 - -1.3 363,452 + +3.3 363,453 - +2.1 363,453 - -0.3 363,453 - -3.2 363,453 - -0.7 363,453 - -0.1 363,453 - +0.8 363,453 - -1.6 363,544 - +0.3 363,606 + -0.3 363,606 + +0.3 363,607 - +4.0 363,658 + -2.0 363,658 + +3.8 363,658 + -0.4 363,658 + -2.5 363,659 - -4.2 363,659 - -1.5 363,659 - +2.7 363,659 - +3.1 363,659 - -1.1 363,839 - -0.9 363,869 - -0.9 363,869 - +0.9 363,876 + +1.0 363,877 - -1.4 363,952 + Psyr_0341 0.30 -1.0 363,952 + Psyr_0341 0.30 -1.6 363,952 + Psyr_0341 0.30 -1.3 363,952 + Psyr_0341 0.30 -1.3 363,953 - Psyr_0341 0.30 -1.2 363,953 - Psyr_0341 0.30 +2.4 363,953 - Psyr_0341 0.30 -2.0 363,953 - Psyr_0341 0.30 -2.1 363,953 - Psyr_0341 0.30 +0.1 363,979 - Psyr_0341 0.41 -0.3 364,113 + +1.2 364,113 + -0.4 364,122 + -1.1 364,122 + +1.7 364,122 + -0.2 364,122 + +3.5 364,301 + -0.2 364,301 + -1.4 364,301 + -0.2 364,301 + -1.9 364,381 + Psyr_0342 0.18 -1.4 364,556 + Psyr_0342 0.35 -0.3 364,556 + Psyr_0342 0.35 -1.6 364,557 - Psyr_0342 0.35 -0.2 364,583 + Psyr_0342 0.38 +0.1 364,583 + Psyr_0342 0.38 +2.7 364,584 - Psyr_0342 0.38 -1.3 364,655 + Psyr_0342 0.45 -1.3 364,662 - Psyr_0342 0.46 +0.1 364,662 - Psyr_0342 0.46 -1.3 364,730 + Psyr_0342 0.53 -0.9 364,730 + Psyr_0342 0.53 +3.5 364,730 + Psyr_0342 0.53 -1.8 364,730 + Psyr_0342 0.53 +2.5 364,730 + Psyr_0342 0.53 +3.5 364,730 + Psyr_0342 0.53 -0.9 364,730 + Psyr_0342 0.53 -0.3 364,730 + Psyr_0342 0.53 -2.3 364,730 + Psyr_0342 0.53 -1.7 364,730 + Psyr_0342 0.53 -1.9 364,730 + Psyr_0342 0.53 -1.2 364,730 + Psyr_0342 0.53 -0.2 364,730 + Psyr_0342 0.53 -1.6 364,730 + Psyr_0342 0.53 +2.9 364,730 + Psyr_0342 0.53 +2.5 364,730 + Psyr_0342 0.53 +2.9 364,731 - Psyr_0342 0.53 +4.8 364,731 - Psyr_0342 0.53 -1.0 364,731 - Psyr_0342 0.53 +2.9 364,731 - Psyr_0342 0.53 +3.8 364,731 - Psyr_0342 0.53 +1.2 364,731 - Psyr_0342 0.53 -0.9 364,731 - Psyr_0342 0.53 -2.3 364,731 - Psyr_0342 0.53 +2.1 364,731 - Psyr_0342 0.53 -1.1 364,731 - Psyr_0342 0.53 -1.0 364,736 + Psyr_0342 0.53 +0.4 364,736 + Psyr_0342 0.53 +1.6 364,736 + Psyr_0342 0.53 -1.1 364,736 + Psyr_0342 0.53 -2.4 364,736 + Psyr_0342 0.53 +1.1 364,736 + Psyr_0342 0.53 +2.1 364,737 - Psyr_0342 0.53 -0.5 364,737 - Psyr_0342 0.53 +2.9 364,737 - Psyr_0342 0.53 -0.9 364,737 - Psyr_0342 0.53 -0.1 364,737 - Psyr_0342 0.53 -0.8 364,793 - Psyr_0342 0.59 +2.5 364,793 - Psyr_0342 0.59 +3.1 364,958 + Psyr_0342 0.76 +3.8 364,958 + Psyr_0342 0.76 +1.1 364,958 + Psyr_0342 0.76 +0.7 364,958 + Psyr_0342 0.76 -1.2 364,958 + Psyr_0342 0.76 -1.1 364,958 + Psyr_0342 0.76 -1.2 364,959 - Psyr_0342 0.76 +3.6 364,959 - Psyr_0342 0.76 -0.7 364,959 - Psyr_0342 0.76 +0.1 364,959 - Psyr_0342 0.76 +0.1 364,959 - Psyr_0342 0.76 +0.6 364,959 - Psyr_0342 0.76 +2.3 364,959 - Psyr_0342 0.76 +1.7 364,959 - Psyr_0342 0.76 -2.0 364,959 - Psyr_0342 0.76 +0.1 364,959 - Psyr_0342 0.76 +3.7 364,960 + Psyr_0342 0.76 -1.6 364,960 + Psyr_0342 0.76 -1.0 364,960 + Psyr_0342 0.76 +1.7 364,960 + Psyr_0342 0.76 -2.1 364,960 + Psyr_0342 0.76 -2.5 364,960 + Psyr_0342 0.76 -3.1 364,961 - Psyr_0342 0.76 +1.7 364,961 - Psyr_0342 0.76 +1.1 364,961 - Psyr_0342 0.76 -0.1 364,961 - Psyr_0342 0.76 -3.3 364,961 - Psyr_0342 0.76 -2.0 364,961 - Psyr_0342 0.76 -1.3 364,970 - Psyr_0342 0.77 -0.3 364,999 + Psyr_0342 0.80 -0.7 364,999 + Psyr_0342 0.80 +2.1 365,000 - Psyr_0342 0.80 -0.9 365,017 + Psyr_0342 0.82 -0.6 365,017 + Psyr_0342 0.82 +4.1 365,017 + Psyr_0342 0.82 +1.1 365,018 - Psyr_0342 0.82 +0.3 365,018 - Psyr_0342 0.82 -1.9 365,019 + Psyr_0342 0.82 -0.4 365,019 + Psyr_0342 0.82 +1.4 365,019 + Psyr_0342 0.82 +3.1 365,019 + Psyr_0342 0.82 +2.5 365,019 + Psyr_0342 0.82 -0.4 365,019 + Psyr_0342 0.82 -1.9 365,019 + Psyr_0342 0.82 -2.2 365,019 + Psyr_0342 0.82 +3.5 365,019 + Psyr_0342 0.82 -0.3 365,019 + Psyr_0342 0.82 -3.1 365,020 - Psyr_0342 0.82 -0.3 365,020 - Psyr_0342 0.82 -1.4 365,020 - Psyr_0342 0.82 +2.5 365,020 - Psyr_0342 0.82 +0.1 365,020 - Psyr_0342 0.82 +2.9 365,020 - Psyr_0342 0.82 -0.7 365,020 - Psyr_0342 0.82 +1.4 365,020 - Psyr_0342 0.82 +1.6 365,020 - Psyr_0342 0.82 +0.4 365,020 - Psyr_0342 0.82 -1.3 365,020 - Psyr_0342 0.82 -0.1 365,020 - Psyr_0342 0.82 +0.6 365,020 - Psyr_0342 0.82 +1.1 365,060 + Psyr_0342 0.86 +3.1 365,060 + Psyr_0342 0.86 +0.1 365,060 + Psyr_0342 0.86 -2.3 365,060 + Psyr_0342 0.86 +3.0 365,060 + Psyr_0342 0.86 -1.2 365,060 + Psyr_0342 0.86 +1.1 365,060 + Psyr_0342 0.86 +0.7 365,060 + Psyr_0342 0.86 +1.3 365,061 - Psyr_0342 0.86 -1.5 365,061 - Psyr_0342 0.86 -2.6 365,061 - Psyr_0342 0.86 +0.3 365,061 - Psyr_0342 0.86 -1.2 365,061 - Psyr_0342 0.86 -1.3 365,061 - Psyr_0342 0.86 +0.1 365,063 + Psyr_0342 0.86 -2.0 365,063 + Psyr_0342 0.86 -3.7 365,064 - Psyr_0342 0.86 +3.3
Or see this region's nucleotide sequence