Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0321 and Psyr_0322 overlap by 4 nucleotides Psyr_0322 and Psyr_0323 are separated by 51 nucleotides
Psyr_0321: Psyr_0321 - aminopeptidase P, Metallo peptidase, MEROPS family M24B, at 337,506 to 338,840
_0321
Psyr_0322: Psyr_0322 - 2-octaprenyl-6-methoxyphenol hydroxylase / 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 338,837 to 340,024
_0322
Psyr_0323: Psyr_0323 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase / 2-octaprenyl-6-methoxyphenol hydroxylase, at 340,076 to 341,305
_0323
Position (kb) 338
339
340
341 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 337.887 kb on + strand, within Psyr_0321 at 337.887 kb on + strand, within Psyr_0321 at 338.028 kb on + strand, within Psyr_0321 at 338.028 kb on + strand, within Psyr_0321 at 338.028 kb on + strand, within Psyr_0321 at 338.028 kb on + strand, within Psyr_0321 at 338.072 kb on + strand, within Psyr_0321 at 338.190 kb on + strand, within Psyr_0321 at 338.307 kb on + strand, within Psyr_0321 at 338.308 kb on - strand, within Psyr_0321 at 338.308 kb on - strand, within Psyr_0321 at 338.373 kb on + strand, within Psyr_0321 at 338.373 kb on + strand, within Psyr_0321 at 338.373 kb on + strand, within Psyr_0321 at 338.373 kb on + strand, within Psyr_0321 at 338.373 kb on + strand, within Psyr_0321 at 338.374 kb on - strand, within Psyr_0321 at 338.374 kb on - strand, within Psyr_0321 at 338.535 kb on + strand, within Psyr_0321 at 338.535 kb on + strand, within Psyr_0321 at 338.535 kb on + strand, within Psyr_0321 at 338.535 kb on + strand, within Psyr_0321 at 338.535 kb on + strand, within Psyr_0321 at 338.547 kb on + strand, within Psyr_0321 at 338.547 kb on + strand, within Psyr_0321 at 338.547 kb on + strand, within Psyr_0321 at 338.547 kb on + strand, within Psyr_0321 at 338.547 kb on + strand, within Psyr_0321 at 338.547 kb on + strand, within Psyr_0321 at 338.547 kb on + strand, within Psyr_0321 at 338.547 kb on + strand, within Psyr_0321 at 338.547 kb on + strand, within Psyr_0321 at 338.548 kb on - strand, within Psyr_0321 at 338.601 kb on + strand, within Psyr_0321 at 338.601 kb on + strand, within Psyr_0321 at 338.601 kb on + strand, within Psyr_0321 at 338.601 kb on + strand, within Psyr_0321 at 338.602 kb on - strand, within Psyr_0321 at 338.667 kb on + strand, within Psyr_0321 at 338.667 kb on + strand, within Psyr_0321 at 338.667 kb on + strand, within Psyr_0321 at 338.667 kb on + strand, within Psyr_0321 at 338.667 kb on + strand, within Psyr_0321 at 338.667 kb on + strand, within Psyr_0321 at 338.667 kb on + strand, within Psyr_0321 at 338.667 kb on + strand, within Psyr_0321 at 338.667 kb on + strand, within Psyr_0321 at 338.667 kb on + strand, within Psyr_0321 at 338.667 kb on + strand, within Psyr_0321 at 338.667 kb on + strand, within Psyr_0321 at 338.668 kb on - strand, within Psyr_0321 at 338.668 kb on - strand, within Psyr_0321 at 338.668 kb on - strand, within Psyr_0321 at 338.668 kb on - strand, within Psyr_0321 at 338.711 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 337,887 + Psyr_0321 0.29 +0.4 337,887 + Psyr_0321 0.29 +4.0 338,028 + Psyr_0321 0.39 -1.3 338,028 + Psyr_0321 0.39 -2.1 338,028 + Psyr_0321 0.39 +0.4 338,028 + Psyr_0321 0.39 -0.8 338,072 + Psyr_0321 0.42 +0.2 338,190 + Psyr_0321 0.51 -1.0 338,307 + Psyr_0321 0.60 +2.7 338,308 - Psyr_0321 0.60 -0.9 338,308 - Psyr_0321 0.60 +3.3 338,373 + Psyr_0321 0.65 +3.5 338,373 + Psyr_0321 0.65 -0.3 338,373 + Psyr_0321 0.65 -1.4 338,373 + Psyr_0321 0.65 -1.2 338,373 + Psyr_0321 0.65 +0.9 338,374 - Psyr_0321 0.65 -3.4 338,374 - Psyr_0321 0.65 -0.5 338,535 + Psyr_0321 0.77 -0.1 338,535 + Psyr_0321 0.77 +0.8 338,535 + Psyr_0321 0.77 +2.7 338,535 + Psyr_0321 0.77 -0.8 338,535 + Psyr_0321 0.77 -2.1 338,547 + Psyr_0321 0.78 -0.6 338,547 + Psyr_0321 0.78 -0.1 338,547 + Psyr_0321 0.78 +1.3 338,547 + Psyr_0321 0.78 +0.2 338,547 + Psyr_0321 0.78 -1.2 338,547 + Psyr_0321 0.78 +1.0 338,547 + Psyr_0321 0.78 -0.5 338,547 + Psyr_0321 0.78 +3.0 338,547 + Psyr_0321 0.78 +5.1 338,548 - Psyr_0321 0.78 +0.2 338,601 + Psyr_0321 0.82 -0.2 338,601 + Psyr_0321 0.82 -1.1 338,601 + Psyr_0321 0.82 -1.8 338,601 + Psyr_0321 0.82 +3.5 338,602 - Psyr_0321 0.82 +3.6 338,667 + Psyr_0321 0.87 -1.1 338,667 + Psyr_0321 0.87 +3.7 338,667 + Psyr_0321 0.87 -1.5 338,667 + Psyr_0321 0.87 -0.1 338,667 + Psyr_0321 0.87 +3.5 338,667 + Psyr_0321 0.87 -2.2 338,667 + Psyr_0321 0.87 -1.0 338,667 + Psyr_0321 0.87 +3.2 338,667 + Psyr_0321 0.87 +1.6 338,667 + Psyr_0321 0.87 -4.6 338,667 + Psyr_0321 0.87 -1.0 338,667 + Psyr_0321 0.87 +1.7 338,668 - Psyr_0321 0.87 +2.5 338,668 - Psyr_0321 0.87 +3.0 338,668 - Psyr_0321 0.87 -2.3 338,668 - Psyr_0321 0.87 +3.9 338,711 + +0.4
Or see this region's nucleotide sequence