Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0321

Experiment: KB with Acridine orange 62.5 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0319 and Psyr_0320 are separated by 150 nucleotidesPsyr_0320 and Psyr_0321 are separated by 75 nucleotidesPsyr_0321 and Psyr_0322 overlap by 4 nucleotides Psyr_0319: Psyr_0319 - conserved hypothetical protein, at 336,501 to 336,710 _0319 Psyr_0320: Psyr_0320 - Protein of unknown function UPF0149, at 336,861 to 337,430 _0320 Psyr_0321: Psyr_0321 - aminopeptidase P, Metallo peptidase, MEROPS family M24B, at 337,506 to 338,840 _0321 Psyr_0322: Psyr_0322 - 2-octaprenyl-6-methoxyphenol hydroxylase / 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 338,837 to 340,024 _0322 Position (kb) 337 338 339Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4 5at 336.618 kb on + strand, within Psyr_0319at 336.618 kb on + strand, within Psyr_0319at 336.618 kb on + strand, within Psyr_0319at 336.618 kb on + strand, within Psyr_0319at 336.619 kb on - strand, within Psyr_0319at 336.619 kb on - strand, within Psyr_0319at 336.619 kb on - strand, within Psyr_0319at 336.621 kb on + strand, within Psyr_0319at 336.621 kb on + strand, within Psyr_0319at 336.621 kb on + strand, within Psyr_0319at 336.621 kb on + strand, within Psyr_0319at 336.621 kb on + strand, within Psyr_0319at 336.621 kb on + strand, within Psyr_0319at 336.621 kb on + strand, within Psyr_0319at 336.621 kb on + strand, within Psyr_0319at 336.621 kb on + strand, within Psyr_0319at 336.621 kb on + strand, within Psyr_0319at 336.621 kb on + strand, within Psyr_0319at 336.622 kb on - strand, within Psyr_0319at 336.641 kb on + strand, within Psyr_0319at 336.655 kb on + strand, within Psyr_0319at 336.655 kb on + strand, within Psyr_0319at 336.655 kb on + strand, within Psyr_0319at 336.656 kb on - strand, within Psyr_0319at 336.656 kb on - strand, within Psyr_0319at 336.656 kb on - strand, within Psyr_0319at 336.656 kb on - strand, within Psyr_0319at 336.725 kb on + strandat 336.725 kb on + strandat 336.725 kb on + strandat 336.725 kb on + strandat 336.808 kb on + strandat 336.813 kb on + strandat 336.813 kb on + strandat 336.813 kb on + strandat 336.813 kb on + strandat 336.829 kb on + strandat 336.829 kb on + strandat 336.829 kb on + strandat 336.853 kb on - strandat 336.856 kb on + strandat 336.882 kb on + strandat 336.920 kb on + strand, within Psyr_0320at 336.925 kb on + strand, within Psyr_0320at 337.332 kb on + strand, within Psyr_0320at 337.350 kb on + strand, within Psyr_0320at 337.350 kb on + strand, within Psyr_0320at 337.350 kb on + strand, within Psyr_0320at 337.350 kb on + strand, within Psyr_0320at 337.350 kb on + strand, within Psyr_0320at 337.350 kb on + strand, within Psyr_0320at 337.350 kb on + strand, within Psyr_0320at 337.350 kb on + strand, within Psyr_0320at 337.350 kb on + strand, within Psyr_0320at 337.350 kb on + strand, within Psyr_0320at 337.350 kb on + strand, within Psyr_0320at 337.350 kb on + strand, within Psyr_0320at 337.351 kb on - strand, within Psyr_0320at 337.351 kb on - strand, within Psyr_0320at 337.530 kb on + strandat 337.716 kb on + strand, within Psyr_0321at 337.725 kb on + strand, within Psyr_0321at 337.725 kb on + strand, within Psyr_0321at 337.725 kb on + strand, within Psyr_0321at 337.725 kb on + strand, within Psyr_0321at 337.725 kb on + strand, within Psyr_0321at 337.725 kb on + strand, within Psyr_0321at 337.725 kb on + strand, within Psyr_0321at 337.725 kb on + strand, within Psyr_0321at 337.725 kb on + strand, within Psyr_0321at 337.725 kb on + strand, within Psyr_0321at 337.725 kb on + strand, within Psyr_0321at 337.729 kb on + strand, within Psyr_0321at 337.729 kb on + strand, within Psyr_0321at 337.729 kb on + strand, within Psyr_0321at 337.729 kb on + strand, within Psyr_0321at 337.729 kb on + strand, within Psyr_0321at 337.729 kb on + strand, within Psyr_0321at 337.729 kb on + strand, within Psyr_0321at 337.730 kb on - strand, within Psyr_0321at 337.730 kb on - strand, within Psyr_0321at 337.730 kb on - strand, within Psyr_0321at 337.730 kb on - strand, within Psyr_0321at 337.730 kb on - strand, within Psyr_0321at 337.730 kb on - strand, within Psyr_0321at 337.887 kb on + strand, within Psyr_0321at 337.887 kb on + strand, within Psyr_0321at 338.028 kb on + strand, within Psyr_0321at 338.028 kb on + strand, within Psyr_0321at 338.028 kb on + strand, within Psyr_0321at 338.028 kb on + strand, within Psyr_0321at 338.072 kb on + strand, within Psyr_0321at 338.190 kb on + strand, within Psyr_0321at 338.307 kb on + strand, within Psyr_0321at 338.308 kb on - strand, within Psyr_0321at 338.308 kb on - strand, within Psyr_0321at 338.373 kb on + strand, within Psyr_0321at 338.373 kb on + strand, within Psyr_0321at 338.373 kb on + strand, within Psyr_0321at 338.373 kb on + strand, within Psyr_0321at 338.373 kb on + strand, within Psyr_0321at 338.374 kb on - strand, within Psyr_0321at 338.374 kb on - strand, within Psyr_0321at 338.535 kb on + strand, within Psyr_0321at 338.535 kb on + strand, within Psyr_0321at 338.535 kb on + strand, within Psyr_0321at 338.535 kb on + strand, within Psyr_0321at 338.535 kb on + strand, within Psyr_0321at 338.547 kb on + strand, within Psyr_0321at 338.547 kb on + strand, within Psyr_0321at 338.547 kb on + strand, within Psyr_0321at 338.547 kb on + strand, within Psyr_0321at 338.547 kb on + strand, within Psyr_0321at 338.547 kb on + strand, within Psyr_0321at 338.547 kb on + strand, within Psyr_0321at 338.547 kb on + strand, within Psyr_0321at 338.547 kb on + strand, within Psyr_0321at 338.548 kb on - strand, within Psyr_0321at 338.601 kb on + strand, within Psyr_0321at 338.601 kb on + strand, within Psyr_0321at 338.601 kb on + strand, within Psyr_0321at 338.601 kb on + strand, within Psyr_0321at 338.602 kb on - strand, within Psyr_0321at 338.667 kb on + strand, within Psyr_0321at 338.667 kb on + strand, within Psyr_0321at 338.667 kb on + strand, within Psyr_0321at 338.667 kb on + strand, within Psyr_0321at 338.667 kb on + strand, within Psyr_0321at 338.667 kb on + strand, within Psyr_0321at 338.667 kb on + strand, within Psyr_0321at 338.667 kb on + strand, within Psyr_0321at 338.667 kb on + strand, within Psyr_0321at 338.667 kb on + strand, within Psyr_0321at 338.667 kb on + strand, within Psyr_0321at 338.667 kb on + strand, within Psyr_0321at 338.668 kb on - strand, within Psyr_0321at 338.668 kb on - strand, within Psyr_0321at 338.668 kb on - strand, within Psyr_0321at 338.668 kb on - strand, within Psyr_0321at 338.711 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml
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336,618 + Psyr_0319 0.56 -1.4
336,618 + Psyr_0319 0.56 +2.2
336,618 + Psyr_0319 0.56 +0.2
336,618 + Psyr_0319 0.56 +2.2
336,619 - Psyr_0319 0.56 +2.5
336,619 - Psyr_0319 0.56 -2.1
336,619 - Psyr_0319 0.56 -1.8
336,621 + Psyr_0319 0.57 -3.3
336,621 + Psyr_0319 0.57 -5.3
336,621 + Psyr_0319 0.57 -3.6
336,621 + Psyr_0319 0.57 +0.2
336,621 + Psyr_0319 0.57 -5.0
336,621 + Psyr_0319 0.57 -1.4
336,621 + Psyr_0319 0.57 +0.2
336,621 + Psyr_0319 0.57 +1.2
336,621 + Psyr_0319 0.57 +2.2
336,621 + Psyr_0319 0.57 +0.2
336,621 + Psyr_0319 0.57 +1.2
336,622 - Psyr_0319 0.58 -1.3
336,641 + Psyr_0319 0.67 -2.1
336,655 + Psyr_0319 0.73 -3.6
336,655 + Psyr_0319 0.73 +1.2
336,655 + Psyr_0319 0.73 -2.9
336,656 - Psyr_0319 0.74 -3.1
336,656 - Psyr_0319 0.74 -0.8
336,656 - Psyr_0319 0.74 -1.2
336,656 - Psyr_0319 0.74 +1.7
336,725 + +1.2
336,725 + +3.0
336,725 + +2.7
336,725 + -0.6
336,808 + -1.6
336,813 + +2.2
336,813 + +2.5
336,813 + -0.2
336,813 + -0.8
336,829 + -1.5
336,829 + -0.6
336,829 + +3.2
336,853 - -3.5
336,856 + +2.7
336,882 + +2.2
336,920 + Psyr_0320 0.10 +2.7
336,925 + Psyr_0320 0.11 -1.3
337,332 + Psyr_0320 0.83 -0.8
337,350 + Psyr_0320 0.86 -1.7
337,350 + Psyr_0320 0.86 +0.2
337,350 + Psyr_0320 0.86 -3.6
337,350 + Psyr_0320 0.86 +0.7
337,350 + Psyr_0320 0.86 +2.5
337,350 + Psyr_0320 0.86 -0.9
337,350 + Psyr_0320 0.86 -0.8
337,350 + Psyr_0320 0.86 +1.2
337,350 + Psyr_0320 0.86 +0.8
337,350 + Psyr_0320 0.86 +2.2
337,350 + Psyr_0320 0.86 +1.2
337,350 + Psyr_0320 0.86 -0.7
337,351 - Psyr_0320 0.86 +1.2
337,351 - Psyr_0320 0.86 -0.2
337,530 + +2.7
337,716 + Psyr_0321 0.16 +3.5
337,725 + Psyr_0321 0.16 -0.4
337,725 + Psyr_0321 0.16 +3.0
337,725 + Psyr_0321 0.16 +0.6
337,725 + Psyr_0321 0.16 -1.3
337,725 + Psyr_0321 0.16 +4.3
337,725 + Psyr_0321 0.16 -0.5
337,725 + Psyr_0321 0.16 -1.2
337,725 + Psyr_0321 0.16 +0.2
337,725 + Psyr_0321 0.16 +2.2
337,725 + Psyr_0321 0.16 +4.3
337,725 + Psyr_0321 0.16 +3.5
337,729 + Psyr_0321 0.17 -1.5
337,729 + Psyr_0321 0.17 +3.5
337,729 + Psyr_0321 0.17 -2.4
337,729 + Psyr_0321 0.17 -0.2
337,729 + Psyr_0321 0.17 +3.6
337,729 + Psyr_0321 0.17 -1.6
337,729 + Psyr_0321 0.17 +2.2
337,730 - Psyr_0321 0.17 +0.2
337,730 - Psyr_0321 0.17 -1.1
337,730 - Psyr_0321 0.17 +3.3
337,730 - Psyr_0321 0.17 +2.5
337,730 - Psyr_0321 0.17 -2.7
337,730 - Psyr_0321 0.17 +0.7
337,887 + Psyr_0321 0.29 +0.4
337,887 + Psyr_0321 0.29 +4.0
338,028 + Psyr_0321 0.39 -1.3
338,028 + Psyr_0321 0.39 -2.1
338,028 + Psyr_0321 0.39 +0.4
338,028 + Psyr_0321 0.39 -0.8
338,072 + Psyr_0321 0.42 +0.2
338,190 + Psyr_0321 0.51 -1.0
338,307 + Psyr_0321 0.60 +2.7
338,308 - Psyr_0321 0.60 -0.9
338,308 - Psyr_0321 0.60 +3.3
338,373 + Psyr_0321 0.65 +3.5
338,373 + Psyr_0321 0.65 -0.3
338,373 + Psyr_0321 0.65 -1.4
338,373 + Psyr_0321 0.65 -1.2
338,373 + Psyr_0321 0.65 +0.9
338,374 - Psyr_0321 0.65 -3.4
338,374 - Psyr_0321 0.65 -0.5
338,535 + Psyr_0321 0.77 -0.1
338,535 + Psyr_0321 0.77 +0.8
338,535 + Psyr_0321 0.77 +2.7
338,535 + Psyr_0321 0.77 -0.8
338,535 + Psyr_0321 0.77 -2.1
338,547 + Psyr_0321 0.78 -0.6
338,547 + Psyr_0321 0.78 -0.1
338,547 + Psyr_0321 0.78 +1.3
338,547 + Psyr_0321 0.78 +0.2
338,547 + Psyr_0321 0.78 -1.2
338,547 + Psyr_0321 0.78 +1.0
338,547 + Psyr_0321 0.78 -0.5
338,547 + Psyr_0321 0.78 +3.0
338,547 + Psyr_0321 0.78 +5.1
338,548 - Psyr_0321 0.78 +0.2
338,601 + Psyr_0321 0.82 -0.2
338,601 + Psyr_0321 0.82 -1.1
338,601 + Psyr_0321 0.82 -1.8
338,601 + Psyr_0321 0.82 +3.5
338,602 - Psyr_0321 0.82 +3.6
338,667 + Psyr_0321 0.87 -1.1
338,667 + Psyr_0321 0.87 +3.7
338,667 + Psyr_0321 0.87 -1.5
338,667 + Psyr_0321 0.87 -0.1
338,667 + Psyr_0321 0.87 +3.5
338,667 + Psyr_0321 0.87 -2.2
338,667 + Psyr_0321 0.87 -1.0
338,667 + Psyr_0321 0.87 +3.2
338,667 + Psyr_0321 0.87 +1.6
338,667 + Psyr_0321 0.87 -4.6
338,667 + Psyr_0321 0.87 -1.0
338,667 + Psyr_0321 0.87 +1.7
338,668 - Psyr_0321 0.87 +2.5
338,668 - Psyr_0321 0.87 +3.0
338,668 - Psyr_0321 0.87 -2.3
338,668 - Psyr_0321 0.87 +3.9
338,711 + +0.4

Or see this region's nucleotide sequence