Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0305 and Psyr_0306 are separated by 274 nucleotides Psyr_0306 and Psyr_0307 are separated by 586 nucleotides
Psyr_0305: Psyr_0305 - GlpT transporter:Glycerol-3-phosphate transporter, at 325,019 to 326,368
_0305
Psyr_0306: Psyr_0306 - TonB, C-terminal, at 326,643 to 327,308
_0306
Psyr_0307: Psyr_0307 - hypothetical protein, at 327,895 to 328,398
_0307
Position (kb) 326
327
328 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 325.678 kb on - strand, within Psyr_0305 at 325.701 kb on + strand, within Psyr_0305 at 325.701 kb on + strand, within Psyr_0305 at 325.702 kb on - strand, within Psyr_0305 at 325.702 kb on - strand, within Psyr_0305 at 325.702 kb on - strand, within Psyr_0305 at 325.702 kb on - strand, within Psyr_0305 at 325.797 kb on + strand, within Psyr_0305 at 325.797 kb on + strand, within Psyr_0305 at 325.797 kb on + strand, within Psyr_0305 at 325.797 kb on + strand, within Psyr_0305 at 325.798 kb on - strand, within Psyr_0305 at 325.798 kb on - strand, within Psyr_0305 at 325.798 kb on - strand, within Psyr_0305 at 325.798 kb on - strand, within Psyr_0305 at 326.088 kb on + strand, within Psyr_0305 at 326.089 kb on - strand, within Psyr_0305 at 326.188 kb on + strand, within Psyr_0305 at 326.189 kb on - strand, within Psyr_0305 at 326.189 kb on - strand, within Psyr_0305 at 326.189 kb on - strand, within Psyr_0305 at 326.190 kb on + strand, within Psyr_0305 at 326.208 kb on + strand, within Psyr_0305 at 326.209 kb on - strand, within Psyr_0305 at 326.209 kb on - strand, within Psyr_0305 at 326.244 kb on + strand at 326.244 kb on + strand at 326.245 kb on - strand at 326.245 kb on - strand at 326.245 kb on - strand at 326.245 kb on - strand at 326.302 kb on - strand at 326.384 kb on + strand at 326.385 kb on - strand at 326.385 kb on - strand at 326.459 kb on - strand at 326.523 kb on + strand at 326.539 kb on + strand at 326.539 kb on + strand at 326.587 kb on + strand at 326.587 kb on + strand at 326.588 kb on - strand at 326.636 kb on + strand at 326.636 kb on + strand at 326.637 kb on - strand at 326.637 kb on - strand at 326.637 kb on - strand at 326.721 kb on + strand, within Psyr_0306 at 326.721 kb on + strand, within Psyr_0306 at 326.721 kb on + strand, within Psyr_0306 at 326.721 kb on + strand, within Psyr_0306 at 326.722 kb on - strand, within Psyr_0306 at 326.722 kb on - strand, within Psyr_0306 at 326.722 kb on - strand, within Psyr_0306 at 326.722 kb on - strand, within Psyr_0306 at 326.722 kb on - strand, within Psyr_0306 at 326.892 kb on + strand, within Psyr_0306 at 326.894 kb on + strand, within Psyr_0306 at 326.894 kb on + strand, within Psyr_0306 at 326.894 kb on + strand, within Psyr_0306 at 326.894 kb on + strand, within Psyr_0306 at 326.894 kb on + strand, within Psyr_0306 at 326.895 kb on - strand, within Psyr_0306 at 326.895 kb on - strand, within Psyr_0306 at 327.080 kb on + strand, within Psyr_0306 at 327.099 kb on + strand, within Psyr_0306 at 327.100 kb on - strand, within Psyr_0306 at 327.100 kb on - strand at 327.243 kb on + strand at 327.243 kb on + strand at 327.244 kb on - strand at 327.244 kb on - strand at 327.244 kb on - strand at 327.244 kb on - strand at 327.244 kb on - strand at 327.325 kb on + strand at 327.325 kb on + strand at 327.325 kb on + strand at 327.325 kb on + strand at 327.325 kb on + strand at 327.325 kb on + strand at 327.326 kb on - strand at 327.326 kb on - strand at 327.326 kb on - strand at 327.326 kb on - strand at 327.326 kb on - strand at 327.326 kb on - strand at 327.326 kb on - strand at 327.354 kb on + strand at 327.355 kb on - strand at 327.537 kb on + strand at 327.537 kb on + strand at 327.538 kb on - strand at 327.585 kb on + strand at 327.646 kb on + strand at 327.669 kb on + strand at 327.670 kb on - strand at 327.670 kb on - strand at 327.670 kb on - strand at 327.779 kb on + strand at 327.779 kb on + strand at 327.806 kb on + strand at 327.806 kb on + strand at 327.807 kb on - strand at 327.813 kb on + strand at 327.813 kb on + strand at 327.814 kb on - strand at 327.837 kb on - strand at 327.837 kb on - strand at 327.842 kb on - strand at 327.931 kb on - strand at 327.996 kb on - strand, within Psyr_0307 at 328.012 kb on - strand, within Psyr_0307 at 328.029 kb on - strand, within Psyr_0307 at 328.044 kb on + strand, within Psyr_0307 at 328.047 kb on + strand, within Psyr_0307 at 328.047 kb on + strand, within Psyr_0307 at 328.117 kb on + strand, within Psyr_0307 at 328.121 kb on + strand, within Psyr_0307 at 328.178 kb on - strand, within Psyr_0307 at 328.214 kb on + strand, within Psyr_0307 at 328.214 kb on + strand at 328.214 kb on + strand, within Psyr_0307 at 328.257 kb on + strand, within Psyr_0307 at 328.257 kb on + strand, within Psyr_0307 at 328.257 kb on + strand, within Psyr_0307 at 328.258 kb on - strand, within Psyr_0307 at 328.258 kb on - strand, within Psyr_0307 at 328.294 kb on + strand, within Psyr_0307 at 328.295 kb on - strand, within Psyr_0307
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 325,678 - Psyr_0305 0.49 +2.8 325,701 + Psyr_0305 0.51 -1.1 325,701 + Psyr_0305 0.51 -2.9 325,702 - Psyr_0305 0.51 -0.9 325,702 - Psyr_0305 0.51 +1.0 325,702 - Psyr_0305 0.51 -0.8 325,702 - Psyr_0305 0.51 -3.8 325,797 + Psyr_0305 0.58 -1.8 325,797 + Psyr_0305 0.58 +0.2 325,797 + Psyr_0305 0.58 -0.2 325,797 + Psyr_0305 0.58 -2.8 325,798 - Psyr_0305 0.58 -1.0 325,798 - Psyr_0305 0.58 -0.2 325,798 - Psyr_0305 0.58 +3.8 325,798 - Psyr_0305 0.58 -0.9 326,088 + Psyr_0305 0.79 +3.9 326,089 - Psyr_0305 0.79 -1.6 326,188 + Psyr_0305 0.87 +2.2 326,189 - Psyr_0305 0.87 +1.2 326,189 - Psyr_0305 0.87 -1.3 326,189 - Psyr_0305 0.87 -2.6 326,190 + Psyr_0305 0.87 -1.6 326,208 + Psyr_0305 0.88 +2.2 326,209 - Psyr_0305 0.88 +2.2 326,209 - Psyr_0305 0.88 +0.6 326,244 + -2.0 326,244 + -1.8 326,245 - +4.3 326,245 - +2.2 326,245 - -1.5 326,245 - +2.8 326,302 - -1.4 326,384 + -1.5 326,385 - -0.9 326,385 - -0.3 326,459 - -2.0 326,523 + +3.0 326,539 + -1.8 326,539 + +2.5 326,587 + -2.1 326,587 + -3.4 326,588 - -1.7 326,636 + -0.3 326,636 + -1.5 326,637 - -2.3 326,637 - +3.0 326,637 - -1.2 326,721 + Psyr_0306 0.12 +0.6 326,721 + Psyr_0306 0.12 +0.1 326,721 + Psyr_0306 0.12 -0.4 326,721 + Psyr_0306 0.12 +0.3 326,722 - Psyr_0306 0.12 -3.1 326,722 - Psyr_0306 0.12 +4.5 326,722 - Psyr_0306 0.12 +4.3 326,722 - Psyr_0306 0.12 +3.0 326,722 - Psyr_0306 0.12 -2.3 326,892 + Psyr_0306 0.37 -0.4 326,894 + Psyr_0306 0.38 +2.2 326,894 + Psyr_0306 0.38 +1.3 326,894 + Psyr_0306 0.38 +4.0 326,894 + Psyr_0306 0.38 -1.5 326,894 + Psyr_0306 0.38 +1.8 326,895 - Psyr_0306 0.38 +2.8 326,895 - Psyr_0306 0.38 -0.8 327,080 + Psyr_0306 0.66 -1.4 327,099 + Psyr_0306 0.68 +3.0 327,100 - Psyr_0306 0.69 -0.2 327,100 - +0.2 327,243 + +2.6 327,243 + +3.5 327,244 - -0.5 327,244 - -0.5 327,244 - -1.8 327,244 - -1.1 327,244 - +2.5 327,325 + -3.2 327,325 + -1.4 327,325 + -0.4 327,325 + +1.8 327,325 + -1.8 327,325 + -1.1 327,326 - +0.5 327,326 - -0.7 327,326 - +0.2 327,326 - +4.1 327,326 - +2.5 327,326 - -1.6 327,326 - -0.4 327,354 + +2.5 327,355 - +2.2 327,537 + +0.8 327,537 + -0.4 327,538 - +0.6 327,585 + -0.9 327,646 + +0.5 327,669 + -1.9 327,670 - -1.4 327,670 - +0.3 327,670 - +3.9 327,779 + -1.8 327,779 + -4.4 327,806 + -1.4 327,806 + +0.1 327,807 - -2.0 327,813 + -1.4 327,813 + +0.1 327,814 - -0.7 327,837 - +3.3 327,837 - +0.0 327,842 - -1.0 327,931 - -1.0 327,996 - Psyr_0307 0.20 -1.4 328,012 - Psyr_0307 0.23 -0.4 328,029 - Psyr_0307 0.27 -0.1 328,044 + Psyr_0307 0.30 +2.6 328,047 + Psyr_0307 0.30 +2.2 328,047 + Psyr_0307 0.30 +0.2 328,117 + Psyr_0307 0.44 -1.8 328,121 + Psyr_0307 0.45 +3.0 328,178 - Psyr_0307 0.56 +2.5 328,214 + Psyr_0307 0.63 -2.4 328,214 + -1.8 328,214 + Psyr_0307 0.63 +2.2 328,257 + Psyr_0307 0.72 +0.0 328,257 + Psyr_0307 0.72 -0.8 328,257 + Psyr_0307 0.72 +4.8 328,258 - Psyr_0307 0.72 -1.6 328,258 - Psyr_0307 0.72 -1.6 328,294 + Psyr_0307 0.79 -1.3 328,295 - Psyr_0307 0.79 -0.5
Or see this region's nucleotide sequence