Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0269 and Psyr_0270 are separated by 241 nucleotides Psyr_0270 and Psyr_0271 are separated by 74 nucleotides Psyr_0271 and Psyr_0272 are separated by 36 nucleotides
Psyr_0269: Psyr_0269 - cell division checkpoint GTPase YihA, at 286,741 to 287,448
_0269
Psyr_0270: Psyr_0270 - DNA polymerase I, at 287,690 to 290,455
_0270
Psyr_0271: Psyr_0271 - conserved hypothetical protein, at 290,530 to 290,817
_0271
Psyr_0272: Psyr_0272 - homoserine kinase, at 290,854 to 291,804
_0272
Position (kb) 287
288
289
290
291 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 286.701 kb on + strand at 286.701 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.741 kb on + strand at 287.446 kb on + strand at 287.446 kb on + strand at 287.504 kb on + strand at 287.504 kb on + strand at 287.505 kb on - strand at 287.548 kb on + strand at 287.548 kb on + strand at 287.548 kb on + strand at 287.548 kb on + strand at 287.549 kb on - strand at 287.549 kb on - strand at 287.549 kb on - strand at 287.549 kb on - strand at 287.549 kb on - strand at 287.549 kb on - strand at 287.557 kb on + strand at 287.557 kb on + strand at 287.557 kb on + strand at 287.557 kb on + strand at 287.557 kb on + strand at 287.557 kb on + strand at 287.557 kb on + strand at 287.558 kb on - strand at 287.558 kb on - strand at 287.558 kb on - strand at 287.558 kb on - strand at 287.558 kb on - strand at 287.614 kb on - strand at 287.647 kb on + strand at 287.652 kb on + strand at 287.653 kb on - strand at 287.653 kb on - strand at 287.653 kb on - strand at 287.653 kb on - strand at 287.730 kb on - strand at 287.730 kb on - strand at 287.838 kb on - strand at 288.990 kb on + strand, within Psyr_0270 at 289.086 kb on + strand, within Psyr_0270 at 289.087 kb on - strand, within Psyr_0270 at 289.137 kb on - strand, within Psyr_0270 at 289.140 kb on + strand, within Psyr_0270 at 289.140 kb on + strand, within Psyr_0270 at 289.140 kb on + strand, within Psyr_0270 at 289.141 kb on - strand, within Psyr_0270 at 289.141 kb on - strand, within Psyr_0270 at 289.141 kb on - strand, within Psyr_0270 at 289.141 kb on - strand, within Psyr_0270 at 290.540 kb on + strand at 290.540 kb on + strand at 290.541 kb on - strand at 290.541 kb on - strand at 290.628 kb on + strand, within Psyr_0271 at 290.628 kb on + strand, within Psyr_0271 at 290.628 kb on + strand, within Psyr_0271 at 290.628 kb on + strand, within Psyr_0271 at 290.631 kb on + strand, within Psyr_0271 at 290.631 kb on + strand, within Psyr_0271 at 290.631 kb on + strand, within Psyr_0271 at 290.631 kb on + strand, within Psyr_0271 at 290.687 kb on - strand, within Psyr_0271 at 290.687 kb on - strand, within Psyr_0271 at 290.687 kb on - strand, within Psyr_0271 at 290.745 kb on + strand, within Psyr_0271 at 290.745 kb on + strand, within Psyr_0271 at 290.804 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand at 290.823 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 286,701 + -1.7 286,701 + -0.7 286,738 + -0.1 286,738 + -1.0 286,738 + +0.6 286,738 + -1.6 286,738 + -0.4 286,740 + -2.5 286,740 + +2.2 286,740 + +4.7 286,740 + +1.1 286,740 + -0.6 286,740 + -0.3 286,740 + -0.7 286,740 + -0.7 286,741 + +4.4 287,446 + -2.4 287,446 + +1.8 287,504 + +0.0 287,504 + -0.6 287,505 - -0.2 287,548 + +0.6 287,548 + +1.5 287,548 + -1.1 287,548 + +2.2 287,549 - +2.0 287,549 - -1.3 287,549 - +0.6 287,549 - -0.9 287,549 - +3.9 287,549 - -1.4 287,557 + +3.9 287,557 + -0.7 287,557 + -2.6 287,557 + -1.4 287,557 + -1.5 287,557 + +2.2 287,557 + +1.3 287,558 - -0.1 287,558 - +0.4 287,558 - -0.2 287,558 - +0.9 287,558 - -2.6 287,614 - -0.1 287,647 + +3.5 287,652 + +1.8 287,653 - +2.5 287,653 - -1.8 287,653 - -1.1 287,653 - +2.5 287,730 - -1.5 287,730 - +3.5 287,838 - -2.4 288,990 + Psyr_0270 0.47 +1.8 289,086 + Psyr_0270 0.50 +3.0 289,087 - Psyr_0270 0.51 -0.1 289,137 - Psyr_0270 0.52 -1.4 289,140 + Psyr_0270 0.52 -1.1 289,140 + Psyr_0270 0.52 +3.4 289,140 + Psyr_0270 0.52 -2.4 289,141 - Psyr_0270 0.52 +0.6 289,141 - Psyr_0270 0.52 -1.8 289,141 - Psyr_0270 0.52 -1.3 289,141 - Psyr_0270 0.52 -4.9 290,540 + +3.5 290,540 + -0.5 290,541 - +4.4 290,541 - +1.8 290,628 + Psyr_0271 0.34 +2.5 290,628 + Psyr_0271 0.34 -0.4 290,628 + Psyr_0271 0.34 -0.5 290,628 + Psyr_0271 0.34 +3.6 290,631 + Psyr_0271 0.35 -0.4 290,631 + Psyr_0271 0.35 -1.4 290,631 + Psyr_0271 0.35 -1.7 290,631 + Psyr_0271 0.35 +3.2 290,687 - Psyr_0271 0.55 -1.5 290,687 - Psyr_0271 0.55 +4.4 290,687 - Psyr_0271 0.55 +2.6 290,745 + Psyr_0271 0.75 +3.5 290,745 + Psyr_0271 0.75 +0.5 290,804 + -1.1 290,823 + -1.8 290,823 + -0.6 290,823 + -1.2 290,823 + -0.9 290,823 + -2.1 290,823 + -0.9 290,823 + -1.1 290,823 + +1.2
Or see this region's nucleotide sequence