Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0266 and Psyr_0267 overlap by 4 nucleotides Psyr_0267 and Psyr_0268 are separated by 9 nucleotides Psyr_0268 and Psyr_0269 are separated by 221 nucleotides
Psyr_0266: Psyr_0266 - GGDEF domain protein, at 282,921 to 284,993
_0266
Psyr_0267: Psyr_0267 - Endonuclease/exonuclease/phosphatase, at 284,990 to 285,865
_0267
Psyr_0268: Psyr_0268 - DSBA oxidoreductase, at 285,875 to 286,519
_0268
Psyr_0269: Psyr_0269 - cell division checkpoint GTPase YihA, at 286,741 to 287,448
_0269
Position (kb) 285
286
287 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 284.959 kb on + strand at 284.959 kb on + strand at 284.960 kb on - strand at 284.960 kb on - strand at 284.960 kb on - strand at 285.185 kb on + strand, within Psyr_0267 at 285.185 kb on + strand, within Psyr_0267 at 285.186 kb on - strand, within Psyr_0267 at 285.306 kb on + strand, within Psyr_0267 at 285.306 kb on + strand, within Psyr_0267 at 285.307 kb on - strand, within Psyr_0267 at 285.307 kb on - strand, within Psyr_0267 at 285.467 kb on + strand, within Psyr_0267 at 285.468 kb on - strand, within Psyr_0267 at 285.468 kb on - strand, within Psyr_0267 at 285.468 kb on - strand, within Psyr_0267 at 285.468 kb on - strand, within Psyr_0267 at 285.468 kb on - strand, within Psyr_0267 at 285.468 kb on - strand, within Psyr_0267 at 285.519 kb on + strand, within Psyr_0267 at 285.706 kb on - strand, within Psyr_0267 at 285.714 kb on + strand, within Psyr_0267 at 285.876 kb on + strand at 285.876 kb on + strand at 285.877 kb on - strand at 285.877 kb on - strand at 285.877 kb on - strand at 285.975 kb on + strand, within Psyr_0268 at 286.178 kb on + strand, within Psyr_0268 at 286.425 kb on + strand, within Psyr_0268 at 286.426 kb on - strand, within Psyr_0268 at 286.426 kb on - strand, within Psyr_0268 at 286.426 kb on - strand, within Psyr_0268 at 286.468 kb on + strand at 286.513 kb on + strand at 286.592 kb on + strand at 286.598 kb on - strand at 286.611 kb on + strand at 286.644 kb on + strand at 286.644 kb on + strand at 286.701 kb on + strand at 286.701 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.738 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.740 kb on + strand at 286.741 kb on + strand at 287.446 kb on + strand at 287.446 kb on + strand at 287.504 kb on + strand at 287.504 kb on + strand at 287.505 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 284,959 + +2.2 284,959 + -0.2 284,960 - -0.1 284,960 - +0.3 284,960 - +1.5 285,185 + Psyr_0267 0.22 +2.5 285,185 + Psyr_0267 0.22 +3.2 285,186 - Psyr_0267 0.22 +2.5 285,306 + Psyr_0267 0.36 +2.8 285,306 + Psyr_0267 0.36 -2.7 285,307 - Psyr_0267 0.36 -1.1 285,307 - Psyr_0267 0.36 -3.6 285,467 + Psyr_0267 0.54 -2.1 285,468 - Psyr_0267 0.55 +3.8 285,468 - Psyr_0267 0.55 -1.5 285,468 - Psyr_0267 0.55 -1.0 285,468 - Psyr_0267 0.55 +1.8 285,468 - Psyr_0267 0.55 +3.9 285,468 - Psyr_0267 0.55 +0.4 285,519 + Psyr_0267 0.60 +0.2 285,706 - Psyr_0267 0.82 -3.9 285,714 + Psyr_0267 0.83 -0.4 285,876 + -1.0 285,876 + -1.4 285,877 - -0.9 285,877 - -0.1 285,877 - +1.3 285,975 + Psyr_0268 0.16 -1.2 286,178 + Psyr_0268 0.47 -1.1 286,425 + Psyr_0268 0.85 -1.1 286,426 - Psyr_0268 0.85 +0.2 286,426 - Psyr_0268 0.85 -2.0 286,426 - Psyr_0268 0.85 -2.5 286,468 + +2.8 286,513 + +2.3 286,592 + +3.2 286,598 - -0.4 286,611 + -1.2 286,644 + +0.2 286,644 + -2.1 286,701 + -1.7 286,701 + -0.7 286,738 + -0.1 286,738 + -1.0 286,738 + +0.6 286,738 + -1.6 286,738 + -0.4 286,740 + -2.5 286,740 + +2.2 286,740 + +4.7 286,740 + +1.1 286,740 + -0.6 286,740 + -0.3 286,740 + -0.7 286,740 + -0.7 286,741 + +4.4 287,446 + -2.4 287,446 + +1.8 287,504 + +0.0 287,504 + -0.6 287,505 - -0.2
Or see this region's nucleotide sequence