Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0258 and Psyr_0259 are separated by 151 nucleotides Psyr_0259 and Psyr_0260 are separated by 255 nucleotides Psyr_0260 and Psyr_0261 are separated by 224 nucleotides
Psyr_0258: Psyr_0258 - Two-component response regulator OmpR, at 274,133 to 274,873
_0258
Psyr_0259: Psyr_0259 - Osmolarity sensor protein envZ, at 275,025 to 276,347
_0259
Psyr_0260: Psyr_0260 - conserved hypothetical protein, at 276,603 to 277,070
_0260
Psyr_0261: Psyr_0261 - conserved hypothetical protein, at 277,295 to 277,582
_0261
Position (kb) 275
276
277 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 274.096 kb on + strand at 274.096 kb on + strand at 274.915 kb on + strand at 274.915 kb on + strand at 274.916 kb on - strand at 274.947 kb on + strand at 274.948 kb on - strand at 275.024 kb on + strand at 275.024 kb on + strand at 275.024 kb on + strand at 275.024 kb on + strand at 275.024 kb on + strand at 275.025 kb on - strand at 275.025 kb on - strand at 275.025 kb on - strand at 275.036 kb on + strand at 275.036 kb on + strand at 275.036 kb on + strand at 275.320 kb on - strand, within Psyr_0259 at 276.072 kb on + strand, within Psyr_0259 at 276.073 kb on - strand, within Psyr_0259 at 276.076 kb on + strand, within Psyr_0259 at 276.099 kb on + strand, within Psyr_0259 at 276.099 kb on + strand, within Psyr_0259 at 276.099 kb on + strand, within Psyr_0259 at 276.099 kb on + strand, within Psyr_0259 at 276.099 kb on + strand, within Psyr_0259 at 276.100 kb on - strand, within Psyr_0259 at 276.236 kb on + strand at 276.305 kb on - strand at 276.345 kb on + strand at 276.373 kb on + strand at 276.373 kb on + strand at 276.373 kb on + strand at 276.373 kb on + strand at 276.373 kb on + strand at 276.373 kb on + strand at 276.374 kb on - strand at 276.374 kb on - strand at 276.374 kb on - strand at 276.375 kb on + strand at 276.375 kb on + strand at 276.376 kb on - strand at 276.470 kb on + strand at 276.470 kb on + strand at 276.470 kb on + strand at 276.470 kb on + strand at 276.470 kb on + strand at 276.479 kb on + strand at 276.486 kb on - strand at 276.486 kb on - strand at 276.538 kb on + strand at 276.539 kb on - strand at 276.668 kb on + strand, within Psyr_0260 at 276.668 kb on + strand, within Psyr_0260 at 276.683 kb on - strand, within Psyr_0260 at 276.683 kb on - strand, within Psyr_0260 at 276.683 kb on - strand, within Psyr_0260 at 276.749 kb on - strand, within Psyr_0260 at 276.749 kb on - strand, within Psyr_0260 at 276.803 kb on + strand, within Psyr_0260 at 276.804 kb on - strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 277.071 kb on + strand at 277.187 kb on + strand at 277.187 kb on + strand at 277.187 kb on + strand at 277.243 kb on + strand at 277.244 kb on - strand at 277.263 kb on + strand at 277.263 kb on + strand at 277.263 kb on + strand at 277.267 kb on - strand at 277.331 kb on + strand, within Psyr_0261 at 277.331 kb on + strand, within Psyr_0261 at 277.332 kb on - strand, within Psyr_0261 at 277.332 kb on - strand, within Psyr_0261 at 277.332 kb on - strand, within Psyr_0261
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 274,096 + -1.1 274,096 + +3.0 274,915 + +1.2 274,915 + -0.4 274,916 - +0.2 274,947 + -0.8 274,948 - +1.0 275,024 + -0.3 275,024 + +4.8 275,024 + +0.3 275,024 + +3.5 275,024 + +1.0 275,025 - -2.6 275,025 - -1.1 275,025 - -1.2 275,036 + +3.4 275,036 + +0.3 275,036 + +3.4 275,320 - Psyr_0259 0.22 +0.2 276,072 + Psyr_0259 0.79 -3.3 276,073 - Psyr_0259 0.79 +0.2 276,076 + Psyr_0259 0.79 +0.2 276,099 + Psyr_0259 0.81 +1.2 276,099 + Psyr_0259 0.81 -1.5 276,099 + Psyr_0259 0.81 -2.1 276,099 + Psyr_0259 0.81 +0.2 276,099 + Psyr_0259 0.81 -2.3 276,100 - Psyr_0259 0.81 +0.2 276,236 + -2.6 276,305 - +3.0 276,345 + +0.8 276,373 + -2.4 276,373 + +3.0 276,373 + -0.2 276,373 + +0.2 276,373 + +3.0 276,373 + -1.9 276,374 - -1.7 276,374 - +1.2 276,374 - +0.1 276,375 + -1.2 276,375 + -0.8 276,376 - +0.2 276,470 + -1.5 276,470 + +3.8 276,470 + -0.3 276,470 + +3.8 276,470 + +2.2 276,479 + +1.9 276,486 - -0.9 276,486 - +1.8 276,538 + +3.2 276,539 - +0.4 276,668 + Psyr_0260 0.14 +3.8 276,668 + Psyr_0260 0.14 +0.3 276,683 - Psyr_0260 0.17 +4.3 276,683 - Psyr_0260 0.17 +0.7 276,683 - Psyr_0260 0.17 +4.0 276,749 - Psyr_0260 0.31 -2.6 276,749 - Psyr_0260 0.31 -1.3 276,803 + Psyr_0260 0.43 +0.1 276,804 - Psyr_0260 0.43 +0.3 276,835 + Psyr_0260 0.50 +1.2 276,835 + Psyr_0260 0.50 -2.0 276,835 + Psyr_0260 0.50 +0.4 276,835 + Psyr_0260 0.50 -4.3 276,835 + Psyr_0260 0.50 -0.8 276,835 + Psyr_0260 0.50 +2.5 276,835 + Psyr_0260 0.50 -1.0 276,835 + Psyr_0260 0.50 -0.2 276,835 + Psyr_0260 0.50 +4.6 276,836 - Psyr_0260 0.50 +1.2 276,836 - Psyr_0260 0.50 -0.4 276,836 - Psyr_0260 0.50 +1.8 276,836 - Psyr_0260 0.50 -0.9 276,836 - Psyr_0260 0.50 +1.7 276,836 - Psyr_0260 0.50 +0.5 276,836 - Psyr_0260 0.50 +3.4 276,836 - Psyr_0260 0.50 -1.1 276,836 - Psyr_0260 0.50 +4.0 277,071 + -3.5 277,187 + -0.4 277,187 + +0.2 277,187 + +3.0 277,243 + +0.7 277,244 - -1.7 277,263 + +3.8 277,263 + +0.2 277,263 + +4.0 277,267 - -0.6 277,331 + Psyr_0261 0.12 -1.5 277,331 + Psyr_0261 0.12 +0.3 277,332 - Psyr_0261 0.13 +1.0 277,332 - Psyr_0261 0.13 +0.6 277,332 - Psyr_0261 0.13 -0.5
Or see this region's nucleotide sequence