Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0252 and Psyr_0253 are separated by 37 nucleotides Psyr_0253 and Psyr_0254 are separated by 144 nucleotides
Psyr_0252: Psyr_0252 - adenylate cyclase, at 264,861 to 266,315
_0252
Psyr_0253: Psyr_0253 - acetylornithine deacetylase, at 266,353 to 267,540
_0253
Psyr_0254: Psyr_0254 - N-acetylglutamate synthase, at 267,685 to 268,983
_0254
Position (kb) 266
267
268 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 265.373 kb on + strand, within Psyr_0252 at 265.547 kb on - strand, within Psyr_0252 at 265.547 kb on - strand, within Psyr_0252 at 265.547 kb on - strand, within Psyr_0252 at 265.972 kb on + strand, within Psyr_0252 at 265.973 kb on - strand, within Psyr_0252 at 265.973 kb on - strand, within Psyr_0252 at 266.105 kb on - strand, within Psyr_0252 at 266.105 kb on - strand, within Psyr_0252 at 266.253 kb on - strand at 266.344 kb on + strand at 266.352 kb on + strand at 266.353 kb on - strand at 266.378 kb on + strand at 266.379 kb on - strand at 266.581 kb on + strand, within Psyr_0253 at 266.581 kb on + strand, within Psyr_0253 at 266.581 kb on + strand, within Psyr_0253 at 266.582 kb on - strand, within Psyr_0253 at 266.582 kb on - strand, within Psyr_0253 at 266.582 kb on - strand, within Psyr_0253 at 266.916 kb on + strand, within Psyr_0253 at 267.108 kb on + strand, within Psyr_0253 at 267.108 kb on + strand, within Psyr_0253 at 267.109 kb on - strand, within Psyr_0253 at 267.109 kb on - strand, within Psyr_0253 at 267.109 kb on - strand, within Psyr_0253 at 267.263 kb on - strand, within Psyr_0253 at 267.348 kb on + strand, within Psyr_0253 at 267.362 kb on + strand, within Psyr_0253 at 267.460 kb on + strand at 267.461 kb on - strand at 267.486 kb on + strand at 267.487 kb on - strand at 267.487 kb on - strand at 267.487 kb on - strand at 267.500 kb on + strand at 267.500 kb on + strand at 267.501 kb on - strand at 267.501 kb on - strand at 267.520 kb on + strand at 267.520 kb on + strand at 267.520 kb on + strand at 267.521 kb on - strand at 267.521 kb on - strand at 267.581 kb on + strand at 267.581 kb on + strand at 267.581 kb on + strand at 267.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 265,373 + Psyr_0252 0.35 -1.1 265,547 - Psyr_0252 0.47 +3.0 265,547 - Psyr_0252 0.47 +2.8 265,547 - Psyr_0252 0.47 -2.3 265,972 + Psyr_0252 0.76 +3.0 265,973 - Psyr_0252 0.76 +2.8 265,973 - Psyr_0252 0.76 +4.2 266,105 - Psyr_0252 0.85 -0.0 266,105 - Psyr_0252 0.85 -1.1 266,253 - -1.9 266,344 + +1.2 266,352 + +0.6 266,353 - +1.2 266,378 + +4.8 266,379 - -0.1 266,581 + Psyr_0253 0.19 -1.1 266,581 + Psyr_0253 0.19 +0.8 266,581 + Psyr_0253 0.19 +0.2 266,582 - Psyr_0253 0.19 -2.8 266,582 - Psyr_0253 0.19 -0.5 266,582 - Psyr_0253 0.19 -1.9 266,916 + Psyr_0253 0.47 +5.0 267,108 + Psyr_0253 0.64 -0.9 267,108 + Psyr_0253 0.64 -1.7 267,109 - Psyr_0253 0.64 -1.1 267,109 - Psyr_0253 0.64 +3.7 267,109 - Psyr_0253 0.64 -1.5 267,263 - Psyr_0253 0.77 -0.9 267,348 + Psyr_0253 0.84 +3.0 267,362 + Psyr_0253 0.85 -0.7 267,460 + -1.1 267,461 - +2.2 267,486 + +3.5 267,487 - +1.8 267,487 - +2.8 267,487 - +1.8 267,500 + +2.8 267,500 + -0.6 267,501 - -1.0 267,501 - +2.8 267,520 + -0.6 267,520 + -0.7 267,520 + +1.8 267,521 - +2.5 267,521 - -0.7 267,581 + +1.1 267,581 + -1.9 267,581 + -1.7 267,668 + -2.5
Or see this region's nucleotide sequence