Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0241 and Psyr_0242 are separated by 47 nucleotides Psyr_0242 and Psyr_0243 are separated by 81 nucleotides Psyr_0243 and Psyr_0244 are separated by 116 nucleotides
Psyr_0241: Psyr_0241 - conserved hypothetical protein, at 253,210 to 255,141
_0241
Psyr_0242: Psyr_0242 - Protein of unknown function DUF962, at 255,189 to 255,713
_0242
Psyr_0243: Psyr_0243 - cyclic nucleotide-binding protein, at 255,795 to 256,505
_0243
Psyr_0244: Psyr_0244 - extracellular solute-binding protein, family 1, at 256,622 to 257,623
_0244
Position (kb) 255
256
257 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 255.088 kb on + strand at 255.088 kb on + strand at 255.088 kb on + strand at 255.088 kb on + strand at 255.088 kb on + strand at 255.089 kb on - strand at 255.089 kb on - strand at 255.089 kb on - strand at 255.190 kb on + strand at 255.190 kb on + strand at 255.190 kb on + strand at 255.190 kb on + strand at 255.191 kb on - strand at 255.358 kb on + strand, within Psyr_0242 at 255.358 kb on + strand, within Psyr_0242 at 255.358 kb on + strand, within Psyr_0242 at 255.358 kb on + strand, within Psyr_0242 at 255.359 kb on - strand, within Psyr_0242 at 255.361 kb on + strand, within Psyr_0242 at 255.361 kb on + strand, within Psyr_0242 at 255.362 kb on - strand, within Psyr_0242 at 255.465 kb on - strand, within Psyr_0242 at 255.465 kb on - strand, within Psyr_0242 at 255.520 kb on + strand, within Psyr_0242 at 255.521 kb on - strand, within Psyr_0242 at 255.521 kb on - strand, within Psyr_0242 at 255.521 kb on - strand, within Psyr_0242 at 255.521 kb on - strand, within Psyr_0242 at 255.653 kb on + strand, within Psyr_0242 at 255.654 kb on - strand, within Psyr_0242 at 255.673 kb on + strand at 255.674 kb on - strand at 255.714 kb on + strand at 255.714 kb on + strand at 255.718 kb on - strand at 255.760 kb on + strand at 255.760 kb on + strand at 255.760 kb on + strand at 255.760 kb on + strand at 255.838 kb on - strand at 255.954 kb on + strand, within Psyr_0243 at 255.954 kb on + strand, within Psyr_0243 at 255.954 kb on + strand, within Psyr_0243 at 255.954 kb on + strand, within Psyr_0243 at 255.954 kb on + strand, within Psyr_0243 at 255.955 kb on - strand, within Psyr_0243 at 255.955 kb on - strand, within Psyr_0243 at 255.955 kb on - strand, within Psyr_0243 at 255.955 kb on - strand, within Psyr_0243 at 255.955 kb on - strand, within Psyr_0243 at 255.955 kb on - strand, within Psyr_0243 at 255.955 kb on - strand, within Psyr_0243 at 256.167 kb on + strand, within Psyr_0243 at 256.387 kb on + strand, within Psyr_0243 at 256.388 kb on - strand, within Psyr_0243 at 256.388 kb on - strand, within Psyr_0243 at 256.569 kb on - strand at 256.665 kb on + strand at 256.666 kb on - strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.707 kb on - strand at 256.707 kb on - strand at 256.707 kb on - strand at 256.707 kb on - strand at 256.751 kb on + strand, within Psyr_0244 at 256.751 kb on + strand, within Psyr_0244 at 256.752 kb on - strand, within Psyr_0244 at 256.752 kb on - strand, within Psyr_0244 at 256.901 kb on - strand, within Psyr_0244 at 256.953 kb on - strand, within Psyr_0244 at 257.009 kb on + strand, within Psyr_0244 at 257.010 kb on - strand, within Psyr_0244 at 257.048 kb on + strand, within Psyr_0244 at 257.048 kb on + strand, within Psyr_0244 at 257.048 kb on + strand, within Psyr_0244 at 257.048 kb on + strand, within Psyr_0244 at 257.048 kb on + strand, within Psyr_0244 at 257.048 kb on + strand, within Psyr_0244 at 257.049 kb on - strand, within Psyr_0244 at 257.049 kb on - strand, within Psyr_0244 at 257.049 kb on - strand, within Psyr_0244 at 257.111 kb on + strand, within Psyr_0244 at 257.111 kb on + strand, within Psyr_0244 at 257.111 kb on + strand, within Psyr_0244 at 257.112 kb on - strand, within Psyr_0244 at 257.112 kb on - strand, within Psyr_0244 at 257.112 kb on - strand, within Psyr_0244 at 257.112 kb on - strand, within Psyr_0244 at 257.132 kb on - strand, within Psyr_0244 at 257.280 kb on - strand, within Psyr_0244 at 257.282 kb on + strand, within Psyr_0244 at 257.282 kb on + strand, within Psyr_0244 at 257.282 kb on + strand, within Psyr_0244 at 257.283 kb on - strand, within Psyr_0244 at 257.283 kb on - strand, within Psyr_0244 at 257.283 kb on - strand, within Psyr_0244 at 257.285 kb on + strand, within Psyr_0244 at 257.285 kb on + strand, within Psyr_0244 at 257.285 kb on + strand, within Psyr_0244 at 257.285 kb on + strand, within Psyr_0244 at 257.285 kb on - strand, within Psyr_0244 at 257.286 kb on - strand, within Psyr_0244 at 257.393 kb on + strand, within Psyr_0244 at 257.393 kb on + strand, within Psyr_0244 at 257.393 kb on + strand, within Psyr_0244 at 257.393 kb on + strand, within Psyr_0244 at 257.393 kb on + strand, within Psyr_0244 at 257.394 kb on - strand, within Psyr_0244 at 257.394 kb on - strand, within Psyr_0244 at 257.394 kb on - strand, within Psyr_0244 at 257.394 kb on - strand, within Psyr_0244 at 257.394 kb on - strand, within Psyr_0244 at 257.394 kb on - strand, within Psyr_0244 at 257.394 kb on - strand, within Psyr_0244
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 255,088 + -3.1 255,088 + +2.2 255,088 + -0.0 255,088 + +0.1 255,088 + -1.6 255,089 - +2.2 255,089 - +0.5 255,089 - -2.8 255,190 + +2.2 255,190 + +1.2 255,190 + +1.0 255,190 + -0.8 255,191 - +0.7 255,358 + Psyr_0242 0.32 -1.6 255,358 + Psyr_0242 0.32 -0.8 255,358 + Psyr_0242 0.32 -0.2 255,358 + Psyr_0242 0.32 -0.1 255,359 - Psyr_0242 0.32 -0.3 255,361 + Psyr_0242 0.33 +0.2 255,361 + Psyr_0242 0.33 -1.4 255,362 - Psyr_0242 0.33 +0.6 255,465 - Psyr_0242 0.53 +3.2 255,465 - Psyr_0242 0.53 +1.8 255,520 + Psyr_0242 0.63 +4.9 255,521 - Psyr_0242 0.63 +0.1 255,521 - Psyr_0242 0.63 -0.6 255,521 - Psyr_0242 0.63 +3.9 255,521 - Psyr_0242 0.63 +1.8 255,653 + Psyr_0242 0.88 -1.4 255,654 - Psyr_0242 0.89 +0.8 255,673 + +1.8 255,674 - -0.9 255,714 + +3.2 255,714 + +0.9 255,718 - +0.5 255,760 + +2.8 255,760 + +1.6 255,760 + +1.8 255,760 + -1.4 255,838 - +4.1 255,954 + Psyr_0243 0.22 +3.0 255,954 + Psyr_0243 0.22 +2.2 255,954 + Psyr_0243 0.22 +4.1 255,954 + Psyr_0243 0.22 +0.2 255,954 + Psyr_0243 0.22 -0.1 255,955 - Psyr_0243 0.23 -0.4 255,955 - Psyr_0243 0.23 +1.2 255,955 - Psyr_0243 0.23 +1.8 255,955 - Psyr_0243 0.23 -1.4 255,955 - Psyr_0243 0.23 -0.4 255,955 - Psyr_0243 0.23 +3.5 255,955 - Psyr_0243 0.23 +2.8 256,167 + Psyr_0243 0.52 +4.1 256,387 + Psyr_0243 0.83 +0.5 256,388 - Psyr_0243 0.83 -0.1 256,388 - Psyr_0243 0.83 +0.4 256,569 - +4.4 256,665 + +3.0 256,666 - -0.6 256,706 + -2.8 256,706 + -3.8 256,706 + -1.7 256,706 + +0.4 256,706 + -1.8 256,706 + -0.4 256,706 + +4.0 256,706 + -0.1 256,706 + +1.8 256,706 + +2.2 256,707 - -2.3 256,707 - -1.9 256,707 - +1.2 256,707 - -2.4 256,751 + Psyr_0244 0.13 +3.4 256,751 + Psyr_0244 0.13 -1.6 256,752 - Psyr_0244 0.13 -1.9 256,752 - Psyr_0244 0.13 +0.2 256,901 - Psyr_0244 0.28 -1.6 256,953 - Psyr_0244 0.33 -0.3 257,009 + Psyr_0244 0.39 -0.7 257,010 - Psyr_0244 0.39 -2.3 257,048 + Psyr_0244 0.43 -1.3 257,048 + Psyr_0244 0.43 -4.4 257,048 + Psyr_0244 0.43 -0.8 257,048 + Psyr_0244 0.43 -1.0 257,048 + Psyr_0244 0.43 -0.1 257,048 + Psyr_0244 0.43 -1.9 257,049 - Psyr_0244 0.43 -1.4 257,049 - Psyr_0244 0.43 +0.7 257,049 - Psyr_0244 0.43 -1.2 257,111 + Psyr_0244 0.49 +3.2 257,111 + Psyr_0244 0.49 -0.1 257,111 + Psyr_0244 0.49 +1.2 257,112 - Psyr_0244 0.49 +0.2 257,112 - Psyr_0244 0.49 -0.3 257,112 - Psyr_0244 0.49 +1.1 257,112 - Psyr_0244 0.49 -1.6 257,132 - Psyr_0244 0.51 +4.4 257,280 - Psyr_0244 0.66 +1.8 257,282 + Psyr_0244 0.66 +2.5 257,282 + Psyr_0244 0.66 +2.2 257,282 + Psyr_0244 0.66 +1.3 257,283 - Psyr_0244 0.66 +2.5 257,283 - Psyr_0244 0.66 +1.7 257,283 - Psyr_0244 0.66 -1.2 257,285 + Psyr_0244 0.66 +2.5 257,285 + Psyr_0244 0.66 +3.2 257,285 + Psyr_0244 0.66 +2.7 257,285 + Psyr_0244 0.66 -1.0 257,285 - Psyr_0244 0.66 +1.1 257,286 - Psyr_0244 0.66 +1.2 257,393 + Psyr_0244 0.77 -2.9 257,393 + Psyr_0244 0.77 -1.8 257,393 + Psyr_0244 0.77 +3.0 257,393 + Psyr_0244 0.77 +0.2 257,393 + Psyr_0244 0.77 +0.8 257,394 - Psyr_0244 0.77 -1.3 257,394 - Psyr_0244 0.77 -2.0 257,394 - Psyr_0244 0.77 +1.8 257,394 - Psyr_0244 0.77 +3.0 257,394 - Psyr_0244 0.77 -1.3 257,394 - Psyr_0244 0.77 +3.8 257,394 - Psyr_0244 0.77 +1.9
Or see this region's nucleotide sequence