Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0233 and Psyr_0234 are separated by 7 nucleotides Psyr_0234 and Psyr_0235 are separated by 157 nucleotides Psyr_0235 and Psyr_0236 overlap by 29 nucleotides Psyr_0236 and Psyr_0237 are separated by 74 nucleotides
Psyr_0233: Psyr_0233 - Protein of unknown function UPF0153, at 248,105 to 248,458
_0233
Psyr_0234: Psyr_0234 - transcriptional regulator, AsnC family, at 248,466 to 248,954
_0234
Psyr_0235: Psyr_0235 - D-amino acid dehydrogenase small subunit, at 249,112 to 250,413
_0235
Psyr_0236: Psyr_0236 - Endoribonuclease L-PSP, at 250,385 to 250,738
_0236
Psyr_0237: Psyr_0237 - alanine racemase, at 250,813 to 251,886
_0237
Position (kb) 249
250
251 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 248.280 kb on + strand, within Psyr_0233 at 248.280 kb on + strand, within Psyr_0233 at 248.280 kb on + strand, within Psyr_0233 at 248.280 kb on + strand, within Psyr_0233 at 248.281 kb on - strand, within Psyr_0233 at 248.281 kb on - strand, within Psyr_0233 at 248.281 kb on - strand, within Psyr_0233 at 248.309 kb on + strand, within Psyr_0233 at 248.309 kb on + strand, within Psyr_0233 at 248.309 kb on + strand, within Psyr_0233 at 248.310 kb on - strand, within Psyr_0233 at 248.310 kb on - strand, within Psyr_0233 at 248.310 kb on - strand, within Psyr_0233 at 248.418 kb on + strand, within Psyr_0233 at 248.418 kb on + strand, within Psyr_0233 at 248.418 kb on + strand, within Psyr_0233 at 248.419 kb on - strand, within Psyr_0233 at 248.419 kb on - strand, within Psyr_0233 at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.426 kb on + strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.427 kb on - strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.428 kb on + strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.429 kb on - strand at 248.448 kb on + strand at 248.448 kb on + strand at 248.449 kb on - strand at 248.449 kb on - strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.454 kb on + strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.455 kb on - strand at 248.456 kb on + strand at 248.456 kb on + strand at 248.456 kb on + strand at 248.456 kb on + strand at 248.456 kb on + strand at 248.457 kb on - strand at 248.457 kb on - strand at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.518 kb on + strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.519 kb on - strand, within Psyr_0234 at 248.614 kb on + strand, within Psyr_0234 at 248.615 kb on - strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.707 kb on + strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.708 kb on - strand, within Psyr_0234 at 248.835 kb on - strand, within Psyr_0234 at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.913 kb on + strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 248.914 kb on - strand at 249.018 kb on - strand at 249.048 kb on + strand at 249.048 kb on + strand at 249.049 kb on - strand at 249.147 kb on + strand at 249.160 kb on + strand at 249.160 kb on + strand at 249.161 kb on - strand at 249.161 kb on - strand at 249.163 kb on + strand at 249.163 kb on + strand at 249.163 kb on + strand at 249.163 kb on + strand at 249.163 kb on + strand at 249.164 kb on - strand at 249.262 kb on + strand, within Psyr_0235 at 249.356 kb on - strand, within Psyr_0235 at 249.358 kb on - strand, within Psyr_0235 at 249.367 kb on + strand, within Psyr_0235 at 249.368 kb on - strand, within Psyr_0235 at 249.412 kb on + strand, within Psyr_0235 at 249.601 kb on + strand, within Psyr_0235 at 249.602 kb on - strand, within Psyr_0235 at 249.813 kb on + strand, within Psyr_0235 at 249.857 kb on - strand, within Psyr_0235 at 249.860 kb on + strand, within Psyr_0235 at 249.860 kb on + strand, within Psyr_0235 at 249.922 kb on + strand, within Psyr_0235 at 250.003 kb on + strand, within Psyr_0235 at 250.003 kb on + strand, within Psyr_0235 at 250.003 kb on + strand, within Psyr_0235 at 250.003 kb on + strand, within Psyr_0235 at 250.003 kb on + strand, within Psyr_0235 at 250.004 kb on - strand, within Psyr_0235 at 250.004 kb on - strand, within Psyr_0235 at 250.004 kb on - strand, within Psyr_0235 at 250.004 kb on - strand, within Psyr_0235 at 250.198 kb on - strand, within Psyr_0235 at 250.347 kb on + strand at 250.347 kb on + strand at 250.347 kb on + strand at 250.347 kb on + strand at 250.347 kb on + strand at 250.348 kb on - strand at 250.348 kb on - strand at 250.348 kb on - strand at 250.365 kb on + strand at 250.451 kb on + strand, within Psyr_0236 at 250.452 kb on - strand, within Psyr_0236 at 250.802 kb on + strand at 250.802 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 248,280 + Psyr_0233 0.49 +3.0 248,280 + Psyr_0233 0.49 +2.2 248,280 + Psyr_0233 0.49 +4.7 248,280 + Psyr_0233 0.49 +0.4 248,281 - Psyr_0233 0.50 -1.4 248,281 - Psyr_0233 0.50 -0.3 248,281 - Psyr_0233 0.50 +1.8 248,309 + Psyr_0233 0.58 -1.0 248,309 + Psyr_0233 0.58 +0.6 248,309 + Psyr_0233 0.58 +0.8 248,310 - Psyr_0233 0.58 -1.3 248,310 - Psyr_0233 0.58 -0.4 248,310 - Psyr_0233 0.58 +0.7 248,418 + Psyr_0233 0.88 +1.0 248,418 + Psyr_0233 0.88 -1.5 248,418 + Psyr_0233 0.88 +3.7 248,419 - Psyr_0233 0.89 +3.3 248,419 - Psyr_0233 0.89 -0.9 248,426 + +4.2 248,426 + +4.4 248,426 + -1.1 248,426 + -2.3 248,426 + -0.4 248,426 + +2.2 248,426 + +2.2 248,426 + +3.2 248,426 + -0.7 248,426 + -0.2 248,426 + -0.8 248,426 + +2.2 248,426 + -2.1 248,426 + +2.5 248,427 - +2.8 248,427 - +3.7 248,427 - -1.4 248,427 - -3.3 248,427 - -0.3 248,427 - +3.5 248,427 - +0.5 248,427 - +2.2 248,427 - +0.2 248,427 - -4.0 248,427 - -0.3 248,427 - -0.4 248,427 - +1.8 248,427 - +3.5 248,428 + -0.4 248,428 + -0.9 248,428 + +1.8 248,428 + +1.8 248,428 + -2.8 248,428 + +2.5 248,428 + +2.2 248,428 + -1.5 248,429 - -0.4 248,429 - +0.2 248,429 - +0.8 248,429 - -0.8 248,429 - +0.4 248,429 - +3.4 248,429 - -0.8 248,429 - -2.4 248,429 - -1.2 248,448 + -0.7 248,448 + -1.6 248,449 - -4.6 248,449 - -0.4 248,454 + -0.9 248,454 + +4.1 248,454 + +0.4 248,454 + +3.9 248,454 + +0.7 248,454 + -0.1 248,454 + -0.4 248,455 - +3.5 248,455 - -0.2 248,455 - +0.4 248,455 - -1.0 248,455 - +0.0 248,455 - +1.2 248,456 + +0.0 248,456 + -0.9 248,456 + -1.2 248,456 + -0.5 248,456 + +0.7 248,457 - +4.1 248,457 - -1.3 248,518 + Psyr_0234 0.11 +1.4 248,518 + Psyr_0234 0.11 +2.2 248,518 + Psyr_0234 0.11 -1.3 248,518 + Psyr_0234 0.11 +2.2 248,518 + Psyr_0234 0.11 +0.2 248,518 + Psyr_0234 0.11 +2.5 248,518 + Psyr_0234 0.11 +0.4 248,518 + Psyr_0234 0.11 -3.7 248,518 + Psyr_0234 0.11 +2.8 248,518 + Psyr_0234 0.11 -2.5 248,518 + Psyr_0234 0.11 -0.6 248,518 + Psyr_0234 0.11 -1.6 248,519 - Psyr_0234 0.11 +1.0 248,519 - Psyr_0234 0.11 -2.1 248,519 - Psyr_0234 0.11 -1.4 248,519 - Psyr_0234 0.11 -2.1 248,519 - Psyr_0234 0.11 +0.2 248,519 - Psyr_0234 0.11 -2.4 248,519 - Psyr_0234 0.11 -2.1 248,519 - Psyr_0234 0.11 -1.2 248,519 - Psyr_0234 0.11 -1.0 248,519 - Psyr_0234 0.11 -2.0 248,519 - Psyr_0234 0.11 +2.2 248,519 - Psyr_0234 0.11 -1.6 248,614 + Psyr_0234 0.30 +2.2 248,615 - Psyr_0234 0.30 -1.2 248,707 + Psyr_0234 0.49 -2.1 248,707 + Psyr_0234 0.49 -2.4 248,707 + Psyr_0234 0.49 -1.4 248,707 + Psyr_0234 0.49 +3.9 248,707 + Psyr_0234 0.49 -1.1 248,708 - Psyr_0234 0.49 +3.5 248,708 - Psyr_0234 0.49 +3.2 248,708 - Psyr_0234 0.49 +3.7 248,708 - Psyr_0234 0.49 +2.5 248,708 - Psyr_0234 0.49 -2.1 248,835 - Psyr_0234 0.75 +0.2 248,913 + +3.2 248,913 + -0.3 248,913 + +1.2 248,913 + +1.2 248,913 + -1.2 248,913 + +4.3 248,913 + +0.2 248,913 + -0.8 248,913 + -0.2 248,913 + -1.2 248,913 + -0.8 248,914 - +1.9 248,914 - +2.5 248,914 - -0.8 248,914 - -0.3 248,914 - +2.5 248,914 - +1.8 248,914 - -3.3 248,914 - +0.2 248,914 - -2.4 248,914 - -2.2 249,018 - -0.8 249,048 + +1.8 249,048 + -3.8 249,049 - -2.6 249,147 + +2.8 249,160 + -2.9 249,160 + +1.8 249,161 - -1.2 249,161 - -1.7 249,163 + +3.5 249,163 + +2.8 249,163 + -1.2 249,163 + +4.2 249,163 + -0.3 249,164 - -1.1 249,262 + Psyr_0235 0.12 -1.0 249,356 - Psyr_0235 0.19 -0.7 249,358 - Psyr_0235 0.19 +2.5 249,367 + Psyr_0235 0.20 -1.4 249,368 - Psyr_0235 0.20 -2.3 249,412 + Psyr_0235 0.23 -1.5 249,601 + Psyr_0235 0.38 -2.0 249,602 - Psyr_0235 0.38 +2.2 249,813 + Psyr_0235 0.54 -2.4 249,857 - Psyr_0235 0.57 +0.9 249,860 + Psyr_0235 0.57 +2.2 249,860 + Psyr_0235 0.57 +1.2 249,922 + Psyr_0235 0.62 -2.1 250,003 + Psyr_0235 0.68 +1.8 250,003 + Psyr_0235 0.68 +3.0 250,003 + Psyr_0235 0.68 -3.1 250,003 + Psyr_0235 0.68 +1.2 250,003 + Psyr_0235 0.68 -0.5 250,004 - Psyr_0235 0.69 +0.2 250,004 - Psyr_0235 0.69 +0.2 250,004 - Psyr_0235 0.69 -1.6 250,004 - Psyr_0235 0.69 -2.4 250,198 - Psyr_0235 0.83 -2.3 250,347 + -3.0 250,347 + +2.2 250,347 + -1.3 250,347 + -2.8 250,347 + +1.2 250,348 - +0.2 250,348 - -1.8 250,348 - +0.2 250,365 + -0.6 250,451 + Psyr_0236 0.19 +3.4 250,452 - Psyr_0236 0.19 +2.2 250,802 + +1.8 250,802 + +0.8
Or see this region's nucleotide sequence