Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0229 and Psyr_0230 are separated by 72 nucleotides Psyr_0230 and Psyr_0231 are separated by 175 nucleotides Psyr_0231 and Psyr_0232 are separated by 2 nucleotides
Psyr_0229: Psyr_0229 - Phospholipase D/Transphosphatidylase, at 243,182 to 244,762
_0229
Psyr_0230: Psyr_0230 - transcriptional regulator, GntR family, at 244,835 to 246,256
_0230
Psyr_0231: Psyr_0231 - conserved hypothetical protein, at 246,432 to 246,683
_0231
Psyr_0232: Psyr_0232 - gamma-glutamylputrescine oxidase, at 246,686 to 247,999
_0232
Position (kb)
244
245
246
247 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 243.860 kb on + strand, within Psyr_0229 at 243.861 kb on - strand, within Psyr_0229 at 243.861 kb on - strand, within Psyr_0229 at 243.894 kb on + strand, within Psyr_0229 at 243.894 kb on + strand, within Psyr_0229 at 243.894 kb on + strand, within Psyr_0229 at 243.894 kb on + strand, within Psyr_0229 at 243.895 kb on - strand, within Psyr_0229 at 243.895 kb on - strand, within Psyr_0229 at 243.895 kb on - strand, within Psyr_0229 at 243.895 kb on - strand, within Psyr_0229 at 243.895 kb on - strand, within Psyr_0229 at 243.945 kb on + strand, within Psyr_0229 at 243.945 kb on + strand, within Psyr_0229 at 243.945 kb on + strand, within Psyr_0229 at 243.945 kb on + strand, within Psyr_0229 at 243.946 kb on - strand, within Psyr_0229 at 243.963 kb on + strand, within Psyr_0229 at 243.963 kb on + strand, within Psyr_0229 at 243.963 kb on + strand, within Psyr_0229 at 243.964 kb on - strand, within Psyr_0229 at 243.964 kb on - strand, within Psyr_0229 at 243.964 kb on - strand, within Psyr_0229 at 243.964 kb on - strand, within Psyr_0229 at 244.053 kb on + strand, within Psyr_0229 at 244.053 kb on + strand, within Psyr_0229 at 244.053 kb on + strand, within Psyr_0229 at 244.053 kb on + strand, within Psyr_0229 at 244.054 kb on - strand, within Psyr_0229 at 244.054 kb on - strand, within Psyr_0229 at 244.054 kb on - strand, within Psyr_0229 at 244.054 kb on - strand, within Psyr_0229 at 244.054 kb on - strand, within Psyr_0229 at 244.054 kb on - strand, within Psyr_0229 at 244.096 kb on - strand, within Psyr_0229 at 244.188 kb on + strand, within Psyr_0229 at 244.188 kb on + strand, within Psyr_0229 at 244.189 kb on - strand, within Psyr_0229 at 244.189 kb on - strand, within Psyr_0229 at 244.189 kb on - strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.506 kb on + strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.507 kb on - strand, within Psyr_0229 at 244.509 kb on + strand, within Psyr_0229 at 244.509 kb on + strand, within Psyr_0229 at 244.509 kb on + strand, within Psyr_0229 at 244.510 kb on - strand, within Psyr_0229 at 244.510 kb on - strand, within Psyr_0229 at 244.756 kb on + strand at 244.756 kb on + strand at 244.801 kb on + strand at 244.802 kb on - strand at 244.945 kb on - strand at 244.966 kb on + strand at 244.967 kb on - strand at 245.100 kb on + strand, within Psyr_0230 at 245.101 kb on - strand, within Psyr_0230 at 245.101 kb on - strand, within Psyr_0230 at 245.175 kb on + strand, within Psyr_0230 at 245.175 kb on + strand, within Psyr_0230 at 245.175 kb on + strand, within Psyr_0230 at 245.244 kb on + strand, within Psyr_0230 at 245.244 kb on + strand, within Psyr_0230 at 245.244 kb on + strand, within Psyr_0230 at 245.244 kb on + strand, within Psyr_0230 at 245.244 kb on + strand, within Psyr_0230 at 245.244 kb on + strand, within Psyr_0230 at 245.244 kb on + strand, within Psyr_0230 at 245.244 kb on + strand, within Psyr_0230 at 245.244 kb on + strand, within Psyr_0230 at 245.245 kb on - strand, within Psyr_0230 at 245.245 kb on - strand, within Psyr_0230 at 245.245 kb on - strand, within Psyr_0230 at 245.388 kb on + strand, within Psyr_0230 at 245.388 kb on + strand, within Psyr_0230 at 245.394 kb on + strand, within Psyr_0230 at 245.394 kb on + strand, within Psyr_0230 at 245.394 kb on + strand, within Psyr_0230 at 245.395 kb on - strand, within Psyr_0230 at 245.409 kb on + strand, within Psyr_0230 at 245.409 kb on + strand, within Psyr_0230 at 245.409 kb on + strand, within Psyr_0230 at 245.409 kb on + strand, within Psyr_0230 at 245.409 kb on + strand, within Psyr_0230 at 245.409 kb on + strand, within Psyr_0230 at 245.410 kb on - strand, within Psyr_0230 at 245.410 kb on - strand, within Psyr_0230 at 245.410 kb on - strand, within Psyr_0230 at 245.410 kb on - strand, within Psyr_0230 at 245.410 kb on - strand, within Psyr_0230 at 245.410 kb on - strand, within Psyr_0230 at 245.490 kb on + strand, within Psyr_0230 at 245.490 kb on + strand, within Psyr_0230 at 245.490 kb on + strand, within Psyr_0230 at 245.491 kb on - strand, within Psyr_0230 at 245.491 kb on - strand, within Psyr_0230 at 245.491 kb on - strand, within Psyr_0230 at 245.820 kb on + strand, within Psyr_0230 at 245.820 kb on + strand, within Psyr_0230 at 245.821 kb on - strand, within Psyr_0230 at 245.835 kb on + strand, within Psyr_0230 at 245.995 kb on + strand, within Psyr_0230 at 246.067 kb on - strand, within Psyr_0230 at 246.137 kb on - strand at 246.137 kb on - strand at 246.199 kb on - strand at 246.199 kb on - strand at 246.199 kb on - strand at 246.246 kb on + strand at 246.246 kb on + strand at 246.246 kb on + strand at 246.246 kb on + strand at 246.246 kb on + strand at 246.247 kb on - strand at 246.261 kb on + strand at 246.310 kb on - strand at 246.311 kb on + strand at 246.312 kb on - strand at 246.312 kb on - strand at 246.312 kb on - strand at 246.350 kb on + strand at 246.351 kb on - strand at 246.562 kb on - strand, within Psyr_0231 at 246.562 kb on - strand, within Psyr_0231 at 246.562 kb on - strand, within Psyr_0231 at 246.876 kb on + strand, within Psyr_0232 at 246.876 kb on + strand, within Psyr_0232 at 246.877 kb on - strand, within Psyr_0232 at 246.877 kb on - strand, within Psyr_0232 at 246.888 kb on + strand, within Psyr_0232 at 246.888 kb on + strand, within Psyr_0232 at 246.888 kb on + strand, within Psyr_0232 at 246.889 kb on - strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.893 kb on + strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.894 kb on - strand, within Psyr_0232 at 246.920 kb on + strand, within Psyr_0232 at 246.921 kb on - strand, within Psyr_0232 at 247.026 kb on + strand, within Psyr_0232 at 247.026 kb on + strand, within Psyr_0232 at 247.026 kb on + strand, within Psyr_0232 at 247.027 kb on - strand, within Psyr_0232 at 247.027 kb on - strand, within Psyr_0232 at 247.027 kb on - strand, within Psyr_0232 at 247.107 kb on + strand, within Psyr_0232 at 247.107 kb on + strand, within Psyr_0232 at 247.108 kb on - strand, within Psyr_0232 at 247.108 kb on - strand, within Psyr_0232 at 247.110 kb on + strand, within Psyr_0232 at 247.110 kb on + strand, within Psyr_0232 at 247.111 kb on - strand, within Psyr_0232 at 247.137 kb on - strand, within Psyr_0232 at 247.137 kb on - strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.203 kb on + strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232 at 247.204 kb on - strand, within Psyr_0232
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 243,860 + Psyr_0229 0.43 +1.2 243,861 - Psyr_0229 0.43 +1.8 243,861 - Psyr_0229 0.43 -0.2 243,894 + Psyr_0229 0.45 -1.6 243,894 + Psyr_0229 0.45 -0.1 243,894 + Psyr_0229 0.45 +0.2 243,894 + Psyr_0229 0.45 -1.6 243,895 - Psyr_0229 0.45 -0.4 243,895 - Psyr_0229 0.45 +0.4 243,895 - Psyr_0229 0.45 -1.0 243,895 - Psyr_0229 0.45 -2.5 243,895 - Psyr_0229 0.45 -0.3 243,945 + Psyr_0229 0.48 -0.1 243,945 + Psyr_0229 0.48 +4.5 243,945 + Psyr_0229 0.48 +2.0 243,945 + Psyr_0229 0.48 +0.5 243,946 - Psyr_0229 0.48 +4.5 243,963 + Psyr_0229 0.49 +2.8 243,963 + Psyr_0229 0.49 +2.8 243,963 + Psyr_0229 0.49 +0.9 243,964 - Psyr_0229 0.49 -0.8 243,964 - Psyr_0229 0.49 -2.4 243,964 - Psyr_0229 0.49 -1.4 243,964 - Psyr_0229 0.49 +3.7 244,053 + Psyr_0229 0.55 +2.8 244,053 + Psyr_0229 0.55 -0.3 244,053 + Psyr_0229 0.55 +3.0 244,053 + Psyr_0229 0.55 +0.5 244,054 - Psyr_0229 0.55 +0.0 244,054 - Psyr_0229 0.55 -0.5 244,054 - Psyr_0229 0.55 -1.8 244,054 - Psyr_0229 0.55 +0.2 244,054 - Psyr_0229 0.55 +0.9 244,054 - Psyr_0229 0.55 +0.1 244,096 - Psyr_0229 0.58 -0.5 244,188 + Psyr_0229 0.64 -1.8 244,188 + Psyr_0229 0.64 +2.2 244,189 - Psyr_0229 0.64 -1.7 244,189 - Psyr_0229 0.64 -0.7 244,189 - Psyr_0229 0.64 +0.9 244,506 + Psyr_0229 0.84 -1.4 244,506 + Psyr_0229 0.84 -1.3 244,506 + Psyr_0229 0.84 -1.4 244,506 + Psyr_0229 0.84 -1.7 244,506 + Psyr_0229 0.84 +3.2 244,506 + Psyr_0229 0.84 +3.5 244,506 + Psyr_0229 0.84 -2.1 244,506 + Psyr_0229 0.84 -1.2 244,506 + Psyr_0229 0.84 -3.6 244,506 + Psyr_0229 0.84 -0.1 244,506 + Psyr_0229 0.84 +1.2 244,506 + Psyr_0229 0.84 +3.4 244,506 + Psyr_0229 0.84 -0.2 244,506 + Psyr_0229 0.84 -0.2 244,506 + Psyr_0229 0.84 +2.2 244,506 + Psyr_0229 0.84 -1.3 244,506 + Psyr_0229 0.84 -0.6 244,506 + Psyr_0229 0.84 -1.2 244,506 + Psyr_0229 0.84 -0.8 244,506 + Psyr_0229 0.84 -0.8 244,506 + Psyr_0229 0.84 +1.2 244,506 + Psyr_0229 0.84 -0.4 244,506 + Psyr_0229 0.84 -0.8 244,507 - Psyr_0229 0.84 +0.6 244,507 - Psyr_0229 0.84 +3.8 244,507 - Psyr_0229 0.84 -1.9 244,507 - Psyr_0229 0.84 -0.8 244,507 - Psyr_0229 0.84 +1.2 244,507 - Psyr_0229 0.84 +1.6 244,507 - Psyr_0229 0.84 +1.5 244,507 - Psyr_0229 0.84 +1.3 244,507 - Psyr_0229 0.84 -0.8 244,507 - Psyr_0229 0.84 -1.0 244,507 - Psyr_0229 0.84 -2.0 244,507 - Psyr_0229 0.84 -0.3 244,507 - Psyr_0229 0.84 -2.4 244,507 - Psyr_0229 0.84 -2.6 244,507 - Psyr_0229 0.84 +0.7 244,507 - Psyr_0229 0.84 +3.4 244,507 - Psyr_0229 0.84 +4.3 244,507 - Psyr_0229 0.84 -1.8 244,507 - Psyr_0229 0.84 +4.3 244,507 - Psyr_0229 0.84 +3.0 244,507 - Psyr_0229 0.84 +0.3 244,507 - Psyr_0229 0.84 -2.3 244,507 - Psyr_0229 0.84 +0.1 244,507 - Psyr_0229 0.84 -0.9 244,509 + Psyr_0229 0.84 -0.7 244,509 + Psyr_0229 0.84 -0.1 244,509 + Psyr_0229 0.84 +2.7 244,510 - Psyr_0229 0.84 -2.6 244,510 - Psyr_0229 0.84 -1.6 244,756 + +0.6 244,756 + +0.2 244,801 + -0.6 244,802 - +0.5 244,945 - -0.7 244,966 + -0.2 244,967 - -2.7 245,100 + Psyr_0230 0.19 -0.9 245,101 - Psyr_0230 0.19 -1.0 245,101 - Psyr_0230 0.19 -0.9 245,175 + Psyr_0230 0.24 -0.9 245,175 + Psyr_0230 0.24 +0.9 245,175 + Psyr_0230 0.24 +1.8 245,244 + Psyr_0230 0.29 +0.2 245,244 + Psyr_0230 0.29 -0.9 245,244 + Psyr_0230 0.29 -0.3 245,244 + Psyr_0230 0.29 +2.5 245,244 + Psyr_0230 0.29 -1.3 245,244 + Psyr_0230 0.29 -2.7 245,244 + Psyr_0230 0.29 -0.4 245,244 + Psyr_0230 0.29 -2.1 245,244 + Psyr_0230 0.29 -0.3 245,245 - Psyr_0230 0.29 -2.0 245,245 - Psyr_0230 0.29 -0.8 245,245 - Psyr_0230 0.29 -1.8 245,388 + Psyr_0230 0.39 +3.4 245,388 + Psyr_0230 0.39 +2.5 245,394 + Psyr_0230 0.39 -1.7 245,394 + Psyr_0230 0.39 -1.0 245,394 + Psyr_0230 0.39 -0.1 245,395 - Psyr_0230 0.39 +3.2 245,409 + Psyr_0230 0.40 +1.8 245,409 + Psyr_0230 0.40 +4.2 245,409 + Psyr_0230 0.40 +2.2 245,409 + Psyr_0230 0.40 +4.1 245,409 + Psyr_0230 0.40 -1.1 245,409 + Psyr_0230 0.40 +0.2 245,410 - Psyr_0230 0.40 +3.0 245,410 - Psyr_0230 0.40 -1.4 245,410 - Psyr_0230 0.40 +0.2 245,410 - Psyr_0230 0.40 +0.6 245,410 - Psyr_0230 0.40 +3.7 245,410 - Psyr_0230 0.40 +2.8 245,490 + Psyr_0230 0.46 +0.7 245,490 + Psyr_0230 0.46 +1.8 245,490 + Psyr_0230 0.46 +3.5 245,491 - Psyr_0230 0.46 -2.3 245,491 - Psyr_0230 0.46 -1.4 245,491 - Psyr_0230 0.46 -2.5 245,820 + Psyr_0230 0.69 -0.8 245,820 + Psyr_0230 0.69 -1.1 245,821 - Psyr_0230 0.69 -1.9 245,835 + Psyr_0230 0.70 -1.2 245,995 + Psyr_0230 0.82 -0.3 246,067 - Psyr_0230 0.87 +4.5 246,137 - +4.3 246,137 - +2.8 246,199 - -1.7 246,199 - -0.7 246,199 - -0.7 246,246 + -1.3 246,246 + -2.5 246,246 + -2.6 246,246 + -0.9 246,246 + +2.5 246,247 - -1.5 246,261 + -1.2 246,310 - -0.1 246,311 + -0.8 246,312 - +0.3 246,312 - +0.5 246,312 - -1.4 246,350 + -0.5 246,351 - +2.2 246,562 - Psyr_0231 0.52 +0.2 246,562 - Psyr_0231 0.52 -1.1 246,562 - Psyr_0231 0.52 -1.4 246,876 + Psyr_0232 0.14 -0.1 246,876 + Psyr_0232 0.14 +3.4 246,877 - Psyr_0232 0.15 +0.2 246,877 - Psyr_0232 0.15 -1.5 246,888 + Psyr_0232 0.15 -0.2 246,888 + Psyr_0232 0.15 +0.2 246,888 + Psyr_0232 0.15 -0.6 246,889 - Psyr_0232 0.15 +4.2 246,893 + Psyr_0232 0.16 +0.2 246,893 + Psyr_0232 0.16 +2.8 246,893 + Psyr_0232 0.16 +3.7 246,893 + Psyr_0232 0.16 +3.0 246,893 + Psyr_0232 0.16 -1.5 246,893 + Psyr_0232 0.16 -0.4 246,893 + Psyr_0232 0.16 -0.6 246,894 - Psyr_0232 0.16 -0.4 246,894 - Psyr_0232 0.16 -0.6 246,894 - Psyr_0232 0.16 -2.2 246,894 - Psyr_0232 0.16 -0.1 246,894 - Psyr_0232 0.16 -1.8 246,894 - Psyr_0232 0.16 -0.7 246,894 - Psyr_0232 0.16 -1.4 246,920 + Psyr_0232 0.18 +0.2 246,921 - Psyr_0232 0.18 +0.0 247,026 + Psyr_0232 0.26 +3.0 247,026 + Psyr_0232 0.26 +4.1 247,026 + Psyr_0232 0.26 +1.8 247,027 - Psyr_0232 0.26 -1.5 247,027 - Psyr_0232 0.26 -0.5 247,027 - Psyr_0232 0.26 +3.0 247,107 + Psyr_0232 0.32 +0.2 247,107 + Psyr_0232 0.32 +2.2 247,108 - Psyr_0232 0.32 -0.8 247,108 - Psyr_0232 0.32 -1.3 247,110 + Psyr_0232 0.32 +3.0 247,110 + Psyr_0232 0.32 +4.7 247,111 - Psyr_0232 0.32 -2.3 247,137 - Psyr_0232 0.34 -0.2 247,137 - Psyr_0232 0.34 +3.5 247,203 + Psyr_0232 0.39 -1.3 247,203 + Psyr_0232 0.39 -2.7 247,203 + Psyr_0232 0.39 +4.4 247,203 + Psyr_0232 0.39 +4.7 247,203 + Psyr_0232 0.39 -2.5 247,204 - Psyr_0232 0.39 -1.5 247,204 - Psyr_0232 0.39 +1.2 247,204 - Psyr_0232 0.39 +4.3 247,204 - Psyr_0232 0.39 +2.2 247,204 - Psyr_0232 0.39 +0.4 247,204 - Psyr_0232 0.39 +1.2 247,204 - Psyr_0232 0.39 -1.8 247,204 - Psyr_0232 0.39 +1.2 247,204 - Psyr_0232 0.39 -0.2
Or see this region's nucleotide sequence