Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0213 and Psyr_0214 are separated by 21 nucleotides Psyr_0214 and Psyr_0215 are separated by 69 nucleotides Psyr_0215 and Psyr_0216 are separated by 82 nucleotides Psyr_0216 and Psyr_0217 are separated by 10 nucleotides
Psyr_0213: Psyr_0213 - RNAse PH, at 227,415 to 228,137
_0213
Psyr_0214: Psyr_0214 - conserved hypothetical protein, at 228,159 to 228,527
_0214
Psyr_0215: Psyr_0215 - Exodeoxyribonuclease III xth, at 228,597 to 229,376
_0215
Psyr_0216: Psyr_0216 - orotate phosphoribosyltransferase, at 229,459 to 230,100
_0216
Psyr_0217: Psyr_0217 - conserved hypothetical protein, at 230,111 to 230,743
_0217
Position (kb)
228
229
230 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 227.624 kb on + strand, within Psyr_0213 at 227.637 kb on - strand, within Psyr_0213 at 227.675 kb on + strand, within Psyr_0213 at 227.676 kb on - strand, within Psyr_0213 at 227.676 kb on - strand, within Psyr_0213 at 227.676 kb on - strand, within Psyr_0213 at 227.676 kb on - strand, within Psyr_0213 at 227.754 kb on + strand, within Psyr_0213 at 227.754 kb on + strand, within Psyr_0213 at 227.754 kb on + strand, within Psyr_0213 at 227.754 kb on + strand, within Psyr_0213 at 227.754 kb on + strand, within Psyr_0213 at 227.755 kb on - strand, within Psyr_0213 at 227.755 kb on - strand, within Psyr_0213 at 227.755 kb on - strand, within Psyr_0213 at 227.804 kb on + strand, within Psyr_0213 at 227.805 kb on - strand, within Psyr_0213 at 227.805 kb on - strand, within Psyr_0213 at 227.805 kb on - strand, within Psyr_0213 at 227.805 kb on - strand, within Psyr_0213 at 227.805 kb on - strand, within Psyr_0213 at 227.805 kb on - strand, within Psyr_0213 at 227.805 kb on - strand, within Psyr_0213 at 227.807 kb on + strand, within Psyr_0213 at 227.919 kb on + strand, within Psyr_0213 at 227.919 kb on + strand, within Psyr_0213 at 227.919 kb on + strand, within Psyr_0213 at 227.919 kb on + strand, within Psyr_0213 at 227.920 kb on - strand, within Psyr_0213 at 227.920 kb on - strand, within Psyr_0213 at 227.920 kb on - strand, within Psyr_0213 at 227.920 kb on - strand, within Psyr_0213 at 227.920 kb on - strand, within Psyr_0213 at 227.949 kb on + strand, within Psyr_0213 at 227.950 kb on - strand, within Psyr_0213 at 227.950 kb on - strand, within Psyr_0213 at 228.284 kb on + strand, within Psyr_0214 at 228.284 kb on + strand, within Psyr_0214 at 228.470 kb on - strand, within Psyr_0214 at 228.492 kb on + strand at 228.492 kb on + strand at 228.492 kb on + strand at 228.493 kb on - strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.525 kb on + strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.526 kb on - strand at 228.603 kb on - strand at 228.603 kb on - strand at 228.603 kb on - strand at 228.619 kb on + strand at 228.715 kb on + strand, within Psyr_0215 at 228.716 kb on - strand, within Psyr_0215 at 228.776 kb on - strand, within Psyr_0215 at 228.776 kb on - strand, within Psyr_0215 at 228.928 kb on + strand, within Psyr_0215 at 228.928 kb on + strand, within Psyr_0215 at 228.928 kb on + strand, within Psyr_0215 at 228.928 kb on + strand, within Psyr_0215 at 228.928 kb on + strand, within Psyr_0215 at 228.928 kb on + strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.929 kb on - strand, within Psyr_0215 at 228.943 kb on + strand, within Psyr_0215 at 228.943 kb on + strand, within Psyr_0215 at 228.943 kb on + strand, within Psyr_0215 at 228.944 kb on - strand, within Psyr_0215 at 228.944 kb on - strand, within Psyr_0215 at 228.944 kb on - strand, within Psyr_0215 at 228.944 kb on - strand, within Psyr_0215 at 228.944 kb on - strand, within Psyr_0215 at 228.949 kb on + strand, within Psyr_0215 at 228.950 kb on - strand, within Psyr_0215 at 228.950 kb on - strand, within Psyr_0215 at 228.950 kb on - strand, within Psyr_0215 at 228.950 kb on - strand, within Psyr_0215 at 228.950 kb on - strand, within Psyr_0215 at 228.950 kb on - strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.982 kb on + strand, within Psyr_0215 at 228.983 kb on - strand, within Psyr_0215 at 228.983 kb on - strand, within Psyr_0215 at 228.983 kb on - strand, within Psyr_0215 at 228.983 kb on - strand, within Psyr_0215 at 228.983 kb on - strand, within Psyr_0215 at 229.166 kb on - strand, within Psyr_0215 at 229.166 kb on - strand, within Psyr_0215 at 229.210 kb on + strand, within Psyr_0215 at 229.210 kb on + strand, within Psyr_0215 at 229.211 kb on - strand, within Psyr_0215 at 229.219 kb on + strand, within Psyr_0215 at 229.219 kb on + strand, within Psyr_0215 at 229.219 kb on + strand, within Psyr_0215 at 229.220 kb on - strand, within Psyr_0215 at 229.220 kb on - strand, within Psyr_0215 at 229.278 kb on + strand, within Psyr_0215 at 229.278 kb on + strand, within Psyr_0215 at 229.353 kb on + strand at 229.353 kb on + strand at 229.354 kb on - strand at 229.354 kb on - strand at 229.354 kb on - strand at 229.354 kb on - strand at 229.376 kb on + strand at 229.403 kb on + strand at 229.403 kb on + strand at 229.403 kb on + strand at 229.403 kb on + strand at 229.404 kb on - strand at 229.404 kb on - strand at 229.420 kb on - strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.427 kb on + strand at 229.427 kb on + strand at 229.428 kb on - strand at 229.442 kb on + strand at 229.672 kb on + strand, within Psyr_0216 at 230.238 kb on - strand, within Psyr_0217 at 230.297 kb on + strand, within Psyr_0217 at 230.297 kb on + strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.313 kb on - strand, within Psyr_0217 at 230.365 kb on + strand, within Psyr_0217
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 227,624 + Psyr_0213 0.29 +0.5 227,637 - Psyr_0213 0.31 +2.8 227,675 + Psyr_0213 0.36 +0.1 227,676 - Psyr_0213 0.36 -3.5 227,676 - Psyr_0213 0.36 -0.5 227,676 - Psyr_0213 0.36 -1.2 227,676 - Psyr_0213 0.36 -2.0 227,754 + Psyr_0213 0.47 +1.8 227,754 + Psyr_0213 0.47 +1.8 227,754 + Psyr_0213 0.47 +3.0 227,754 + Psyr_0213 0.47 +3.2 227,754 + Psyr_0213 0.47 +1.8 227,755 - Psyr_0213 0.47 +0.9 227,755 - Psyr_0213 0.47 +4.0 227,755 - Psyr_0213 0.47 +1.2 227,804 + Psyr_0213 0.54 -2.0 227,805 - Psyr_0213 0.54 +1.2 227,805 - Psyr_0213 0.54 -0.5 227,805 - Psyr_0213 0.54 -0.3 227,805 - Psyr_0213 0.54 -0.2 227,805 - Psyr_0213 0.54 +1.2 227,805 - Psyr_0213 0.54 -1.4 227,805 - Psyr_0213 0.54 +2.5 227,807 + Psyr_0213 0.54 +1.8 227,919 + Psyr_0213 0.70 -0.4 227,919 + Psyr_0213 0.70 -2.1 227,919 + Psyr_0213 0.70 -0.6 227,919 + Psyr_0213 0.70 +1.8 227,920 - Psyr_0213 0.70 +2.8 227,920 - Psyr_0213 0.70 +2.5 227,920 - Psyr_0213 0.70 +1.8 227,920 - Psyr_0213 0.70 -0.9 227,920 - Psyr_0213 0.70 -1.7 227,949 + Psyr_0213 0.74 +0.5 227,950 - Psyr_0213 0.74 +1.3 227,950 - Psyr_0213 0.74 +1.0 228,284 + Psyr_0214 0.34 -2.3 228,284 + Psyr_0214 0.34 +3.4 228,470 - Psyr_0214 0.84 +4.2 228,492 + -0.9 228,492 + -0.5 228,492 + -1.0 228,493 - +0.3 228,525 + -1.3 228,525 + -0.2 228,525 + -0.9 228,525 + +0.2 228,525 + -3.0 228,525 + +1.2 228,525 + +0.2 228,525 + -0.1 228,525 + +2.8 228,526 - +2.2 228,526 - -1.5 228,526 - -2.0 228,526 - +2.5 228,526 - +0.2 228,526 - -1.1 228,526 - -0.6 228,526 - +0.3 228,526 - -1.9 228,603 - +1.2 228,603 - -2.6 228,603 - -1.9 228,619 + -4.3 228,715 + Psyr_0215 0.15 +0.2 228,716 - Psyr_0215 0.15 +3.5 228,776 - Psyr_0215 0.23 -0.9 228,776 - Psyr_0215 0.23 +0.2 228,928 + Psyr_0215 0.42 -0.9 228,928 + Psyr_0215 0.42 +1.8 228,928 + Psyr_0215 0.42 +1.2 228,928 + Psyr_0215 0.42 -1.4 228,928 + Psyr_0215 0.42 -4.7 228,928 + Psyr_0215 0.42 -3.2 228,929 - Psyr_0215 0.43 +1.0 228,929 - Psyr_0215 0.43 -2.3 228,929 - Psyr_0215 0.43 -2.8 228,929 - Psyr_0215 0.43 +0.7 228,929 - Psyr_0215 0.43 -4.1 228,929 - Psyr_0215 0.43 +2.8 228,929 - Psyr_0215 0.43 -1.5 228,943 + Psyr_0215 0.44 -5.2 228,943 + Psyr_0215 0.44 +0.2 228,943 + Psyr_0215 0.44 +0.2 228,944 - Psyr_0215 0.44 -1.3 228,944 - Psyr_0215 0.44 +1.2 228,944 - Psyr_0215 0.44 -1.6 228,944 - Psyr_0215 0.44 -3.7 228,944 - Psyr_0215 0.44 +1.8 228,949 + Psyr_0215 0.45 -0.7 228,950 - Psyr_0215 0.45 -0.9 228,950 - Psyr_0215 0.45 +0.7 228,950 - Psyr_0215 0.45 -2.4 228,950 - Psyr_0215 0.45 -1.1 228,950 - Psyr_0215 0.45 +0.2 228,950 - Psyr_0215 0.45 -4.1 228,982 + Psyr_0215 0.49 -2.0 228,982 + Psyr_0215 0.49 +1.2 228,982 + Psyr_0215 0.49 +2.2 228,982 + Psyr_0215 0.49 -4.0 228,982 + Psyr_0215 0.49 -3.6 228,982 + Psyr_0215 0.49 -3.1 228,982 + Psyr_0215 0.49 -3.8 228,982 + Psyr_0215 0.49 -1.6 228,982 + Psyr_0215 0.49 -1.9 228,982 + Psyr_0215 0.49 -2.6 228,982 + Psyr_0215 0.49 +1.2 228,983 - Psyr_0215 0.49 -1.3 228,983 - Psyr_0215 0.49 +0.2 228,983 - Psyr_0215 0.49 -0.2 228,983 - Psyr_0215 0.49 -2.1 228,983 - Psyr_0215 0.49 -2.1 229,166 - Psyr_0215 0.73 +1.8 229,166 - Psyr_0215 0.73 +1.8 229,210 + Psyr_0215 0.79 +1.8 229,210 + Psyr_0215 0.79 -3.1 229,211 - Psyr_0215 0.79 -1.3 229,219 + Psyr_0215 0.80 +2.5 229,219 + Psyr_0215 0.80 -3.0 229,219 + Psyr_0215 0.80 -0.5 229,220 - Psyr_0215 0.80 -0.9 229,220 - Psyr_0215 0.80 +3.5 229,278 + Psyr_0215 0.87 -3.8 229,278 + Psyr_0215 0.87 -5.0 229,353 + -2.4 229,353 + -2.5 229,354 - -1.7 229,354 - -0.2 229,354 - +3.0 229,354 - -1.0 229,376 + +0.2 229,403 + +2.2 229,403 + +2.8 229,403 + +3.0 229,403 + -1.1 229,404 - +1.8 229,404 - +2.5 229,420 - +1.8 229,423 + +1.2 229,423 + -0.1 229,423 + -3.3 229,423 + +0.5 229,423 + +1.8 229,427 + +0.2 229,427 + -1.4 229,428 - +0.2 229,442 + -0.9 229,672 + Psyr_0216 0.33 -2.0 230,238 - Psyr_0217 0.20 -2.5 230,297 + Psyr_0217 0.29 +3.0 230,297 + Psyr_0217 0.29 +5.3 230,298 - Psyr_0217 0.30 +3.9 230,298 - Psyr_0217 0.30 +0.5 230,298 - Psyr_0217 0.30 -0.1 230,298 - Psyr_0217 0.30 +3.9 230,313 - Psyr_0217 0.32 -0.7 230,365 + Psyr_0217 0.40 -1.6
Or see this region's nucleotide sequence