Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0194 and Psyr_0195 are separated by 15 nucleotides Psyr_0195 and Psyr_0196 are separated by 3 nucleotides Psyr_0196 and Psyr_0197 are separated by 397 nucleotides
Psyr_0194: Psyr_0194 - Short-chain dehydrogenase/reductase SDR, at 205,070 to 205,804
_0194
Psyr_0195: Psyr_0195 - conserved hypothetical protein, at 205,820 to 206,563
_0195
Psyr_0196: Psyr_0196 - conserved hypothetical protein, at 206,567 to 209,947
_0196
Psyr_0197: Psyr_0197 - Mg chelatase-related protein, at 210,345 to 211,838
_0197
Position (kb) 206
207
208
209
210 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 205.733 kb on + strand at 205.733 kb on + strand at 205.733 kb on + strand at 205.734 kb on - strand at 205.734 kb on - strand at 205.734 kb on - strand at 205.787 kb on - strand at 205.800 kb on - strand at 205.800 kb on - strand at 205.887 kb on + strand at 205.887 kb on + strand at 205.888 kb on - strand at 205.996 kb on - strand, within Psyr_0195 at 205.996 kb on - strand, within Psyr_0195 at 206.048 kb on + strand, within Psyr_0195 at 206.065 kb on + strand, within Psyr_0195 at 206.065 kb on + strand, within Psyr_0195 at 206.120 kb on + strand, within Psyr_0195 at 206.199 kb on + strand, within Psyr_0195 at 206.232 kb on + strand, within Psyr_0195 at 206.232 kb on + strand, within Psyr_0195 at 206.232 kb on + strand, within Psyr_0195 at 206.233 kb on - strand, within Psyr_0195 at 206.238 kb on + strand, within Psyr_0195 at 206.238 kb on + strand, within Psyr_0195 at 206.238 kb on + strand, within Psyr_0195 at 206.238 kb on + strand, within Psyr_0195 at 206.238 kb on + strand, within Psyr_0195 at 206.238 kb on + strand, within Psyr_0195 at 206.239 kb on - strand, within Psyr_0195 at 206.239 kb on - strand, within Psyr_0195 at 206.270 kb on - strand, within Psyr_0195 at 206.274 kb on + strand, within Psyr_0195 at 206.274 kb on + strand, within Psyr_0195 at 206.275 kb on - strand, within Psyr_0195 at 206.305 kb on + strand, within Psyr_0195 at 206.305 kb on + strand, within Psyr_0195 at 206.379 kb on + strand, within Psyr_0195 at 206.380 kb on - strand, within Psyr_0195 at 206.390 kb on - strand, within Psyr_0195 at 206.533 kb on + strand at 206.563 kb on + strand at 206.564 kb on - strand at 206.653 kb on + strand at 206.654 kb on - strand at 206.669 kb on + strand at 206.674 kb on + strand at 206.683 kb on - strand at 206.713 kb on + strand at 206.714 kb on - strand at 206.735 kb on + strand at 206.735 kb on + strand at 206.736 kb on - strand at 206.736 kb on - strand at 206.736 kb on - strand at 206.736 kb on - strand at 206.747 kb on + strand at 206.747 kb on + strand at 206.747 kb on + strand at 206.747 kb on + strand at 206.748 kb on - strand at 206.748 kb on - strand at 206.748 kb on - strand at 206.748 kb on - strand at 206.752 kb on - strand at 206.752 kb on - strand at 206.752 kb on - strand at 206.752 kb on - strand at 206.907 kb on - strand, within Psyr_0196 at 206.921 kb on + strand, within Psyr_0196 at 206.933 kb on + strand, within Psyr_0196 at 206.934 kb on - strand, within Psyr_0196 at 206.951 kb on + strand, within Psyr_0196 at 206.951 kb on + strand, within Psyr_0196 at 206.951 kb on + strand, within Psyr_0196 at 206.991 kb on - strand, within Psyr_0196 at 207.061 kb on - strand, within Psyr_0196 at 207.064 kb on - strand, within Psyr_0196 at 207.111 kb on - strand, within Psyr_0196 at 207.111 kb on - strand, within Psyr_0196 at 207.139 kb on + strand, within Psyr_0196 at 207.139 kb on + strand, within Psyr_0196 at 207.140 kb on - strand at 207.236 kb on + strand, within Psyr_0196 at 207.237 kb on - strand, within Psyr_0196 at 207.237 kb on - strand, within Psyr_0196 at 207.237 kb on - strand, within Psyr_0196 at 207.278 kb on + strand, within Psyr_0196 at 207.278 kb on + strand, within Psyr_0196 at 207.278 kb on + strand, within Psyr_0196 at 207.282 kb on + strand, within Psyr_0196 at 207.282 kb on + strand, within Psyr_0196 at 207.283 kb on - strand, within Psyr_0196 at 207.293 kb on + strand, within Psyr_0196 at 207.293 kb on + strand, within Psyr_0196 at 207.294 kb on - strand, within Psyr_0196 at 207.294 kb on - strand, within Psyr_0196 at 207.308 kb on + strand, within Psyr_0196 at 207.308 kb on + strand, within Psyr_0196 at 207.311 kb on - strand, within Psyr_0196 at 207.371 kb on - strand, within Psyr_0196 at 207.371 kb on - strand, within Psyr_0196 at 207.431 kb on + strand, within Psyr_0196 at 207.448 kb on + strand, within Psyr_0196 at 207.448 kb on + strand, within Psyr_0196 at 207.449 kb on - strand, within Psyr_0196 at 207.449 kb on - strand, within Psyr_0196 at 207.482 kb on + strand, within Psyr_0196 at 207.548 kb on + strand, within Psyr_0196 at 207.548 kb on + strand, within Psyr_0196 at 207.549 kb on - strand, within Psyr_0196 at 207.549 kb on - strand, within Psyr_0196 at 207.680 kb on + strand, within Psyr_0196 at 207.698 kb on - strand, within Psyr_0196 at 207.703 kb on + strand, within Psyr_0196 at 207.703 kb on + strand, within Psyr_0196 at 207.703 kb on + strand, within Psyr_0196 at 207.703 kb on + strand, within Psyr_0196 at 207.703 kb on + strand, within Psyr_0196 at 207.703 kb on + strand, within Psyr_0196 at 207.703 kb on + strand, within Psyr_0196 at 207.703 kb on + strand, within Psyr_0196 at 207.704 kb on - strand, within Psyr_0196 at 207.704 kb on - strand, within Psyr_0196 at 207.704 kb on - strand, within Psyr_0196 at 207.704 kb on - strand, within Psyr_0196 at 207.704 kb on - strand, within Psyr_0196 at 207.704 kb on - strand, within Psyr_0196 at 207.704 kb on - strand, within Psyr_0196 at 207.704 kb on - strand, within Psyr_0196 at 207.704 kb on - strand, within Psyr_0196 at 207.704 kb on - strand, within Psyr_0196 at 207.704 kb on - strand, within Psyr_0196 at 207.717 kb on + strand, within Psyr_0196 at 207.717 kb on + strand, within Psyr_0196 at 207.718 kb on - strand, within Psyr_0196 at 207.718 kb on - strand, within Psyr_0196 at 207.718 kb on - strand, within Psyr_0196 at 207.718 kb on - strand, within Psyr_0196 at 207.718 kb on - strand, within Psyr_0196 at 207.774 kb on + strand, within Psyr_0196 at 207.775 kb on - strand, within Psyr_0196 at 207.943 kb on - strand, within Psyr_0196 at 208.048 kb on - strand, within Psyr_0196 at 208.048 kb on - strand, within Psyr_0196 at 208.048 kb on - strand, within Psyr_0196 at 208.048 kb on - strand, within Psyr_0196 at 208.159 kb on + strand, within Psyr_0196 at 208.168 kb on + strand, within Psyr_0196 at 208.623 kb on + strand, within Psyr_0196 at 208.623 kb on + strand, within Psyr_0196 at 208.623 kb on + strand, within Psyr_0196 at 208.623 kb on + strand, within Psyr_0196 at 208.628 kb on + strand, within Psyr_0196 at 208.628 kb on + strand, within Psyr_0196 at 208.768 kb on - strand, within Psyr_0196 at 208.998 kb on + strand, within Psyr_0196 at 209.302 kb on + strand, within Psyr_0196 at 209.302 kb on + strand, within Psyr_0196 at 209.302 kb on + strand, within Psyr_0196 at 209.302 kb on + strand, within Psyr_0196 at 209.303 kb on - strand, within Psyr_0196 at 209.303 kb on - strand, within Psyr_0196 at 209.303 kb on - strand, within Psyr_0196 at 209.303 kb on - strand, within Psyr_0196 at 209.422 kb on - strand, within Psyr_0196 at 209.655 kb on + strand at 209.656 kb on - strand at 209.656 kb on - strand at 209.656 kb on - strand at 209.656 kb on - strand at 209.656 kb on - strand at 209.656 kb on - strand at 209.656 kb on - strand at 209.656 kb on - strand at 209.656 kb on - strand at 209.656 kb on - strand at 209.685 kb on + strand at 209.685 kb on + strand at 209.686 kb on - strand at 209.686 kb on - strand at 209.686 kb on - strand at 209.686 kb on - strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.691 kb on + strand at 209.698 kb on - strand at 209.698 kb on - strand at 209.698 kb on - strand at 209.706 kb on + strand at 209.706 kb on + strand at 209.706 kb on + strand at 209.706 kb on + strand at 209.706 kb on + strand at 209.706 kb on + strand at 209.707 kb on - strand at 209.723 kb on - strand at 209.725 kb on - strand at 209.725 kb on - strand at 209.725 kb on - strand at 209.725 kb on - strand at 209.725 kb on - strand at 209.725 kb on - strand at 209.989 kb on + strand at 209.990 kb on - strand at 209.990 kb on - strand at 210.012 kb on - strand at 210.047 kb on + strand at 210.048 kb on - strand at 210.093 kb on - strand at 210.152 kb on + strand at 210.152 kb on + strand at 210.153 kb on - strand at 210.153 kb on - strand at 210.153 kb on - strand at 210.154 kb on + strand at 210.154 kb on + strand at 210.154 kb on + strand at 210.155 kb on - strand at 210.155 kb on - strand at 210.207 kb on + strand at 210.207 kb on + strand at 210.207 kb on + strand at 210.207 kb on + strand at 210.207 kb on + strand at 210.207 kb on + strand at 210.208 kb on - strand at 210.208 kb on - strand at 210.208 kb on - strand at 210.235 kb on + strand at 210.235 kb on + strand at 210.236 kb on - strand at 210.236 kb on - strand at 210.236 kb on - strand at 210.271 kb on - strand at 210.302 kb on + strand at 210.302 kb on + strand at 210.303 kb on - strand at 210.329 kb on + strand at 210.329 kb on + strand at 210.329 kb on + strand at 210.330 kb on - strand at 210.365 kb on + strand at 210.917 kb on + strand, within Psyr_0197
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 205,733 + -0.2 205,733 + -1.7 205,733 + -0.2 205,734 - -0.5 205,734 - -1.4 205,734 - -1.5 205,787 - -0.8 205,800 - -1.2 205,800 - +3.0 205,887 + -0.8 205,887 + +2.8 205,888 - -1.1 205,996 - Psyr_0195 0.24 -0.8 205,996 - Psyr_0195 0.24 -0.4 206,048 + Psyr_0195 0.31 +2.5 206,065 + Psyr_0195 0.33 -0.6 206,065 + Psyr_0195 0.33 -1.7 206,120 + Psyr_0195 0.40 -1.5 206,199 + Psyr_0195 0.51 -2.1 206,232 + Psyr_0195 0.55 -1.3 206,232 + Psyr_0195 0.55 +0.2 206,232 + Psyr_0195 0.55 +2.1 206,233 - Psyr_0195 0.56 -2.9 206,238 + Psyr_0195 0.56 +1.8 206,238 + Psyr_0195 0.56 +1.8 206,238 + Psyr_0195 0.56 -0.9 206,238 + Psyr_0195 0.56 -0.2 206,238 + Psyr_0195 0.56 -1.2 206,238 + Psyr_0195 0.56 -1.0 206,239 - Psyr_0195 0.56 -2.3 206,239 - Psyr_0195 0.56 +1.8 206,270 - Psyr_0195 0.60 -1.3 206,274 + Psyr_0195 0.61 -4.0 206,274 + Psyr_0195 0.61 +0.2 206,275 - Psyr_0195 0.61 -2.0 206,305 + Psyr_0195 0.65 +0.2 206,305 + Psyr_0195 0.65 -1.2 206,379 + Psyr_0195 0.75 +0.6 206,380 - Psyr_0195 0.75 -1.1 206,390 - Psyr_0195 0.77 +3.5 206,533 + -0.9 206,563 + -1.6 206,564 - +2.5 206,653 + -1.5 206,654 - -0.8 206,669 + -0.8 206,674 + +0.2 206,683 - +2.5 206,713 + -4.0 206,714 - +4.1 206,735 + -0.1 206,735 + +0.2 206,736 - +1.4 206,736 - +3.9 206,736 - +3.5 206,736 - -2.5 206,747 + +0.3 206,747 + +1.2 206,747 + -2.5 206,747 + -0.6 206,748 - +1.2 206,748 - +0.5 206,748 - -1.0 206,748 - -1.8 206,752 - -0.8 206,752 - +1.8 206,752 - -1.1 206,752 - +1.8 206,907 - Psyr_0196 0.10 +0.6 206,921 + Psyr_0196 0.10 +4.4 206,933 + Psyr_0196 0.11 +0.2 206,934 - Psyr_0196 0.11 -0.3 206,951 + Psyr_0196 0.11 -0.2 206,951 + Psyr_0196 0.11 -1.5 206,951 + Psyr_0196 0.11 -0.4 206,991 - Psyr_0196 0.13 -1.8 207,061 - Psyr_0196 0.15 -0.1 207,064 - Psyr_0196 0.15 -1.1 207,111 - Psyr_0196 0.16 -1.7 207,111 - Psyr_0196 0.16 -3.2 207,139 + Psyr_0196 0.17 +0.6 207,139 + Psyr_0196 0.17 +0.2 207,140 - +0.2 207,236 + Psyr_0196 0.20 -2.6 207,237 - Psyr_0196 0.20 -3.5 207,237 - Psyr_0196 0.20 -3.7 207,237 - Psyr_0196 0.20 -1.7 207,278 + Psyr_0196 0.21 -0.5 207,278 + Psyr_0196 0.21 +2.8 207,278 + Psyr_0196 0.21 +4.4 207,282 + Psyr_0196 0.21 -1.4 207,282 + Psyr_0196 0.21 -2.7 207,283 - Psyr_0196 0.21 +1.0 207,293 + Psyr_0196 0.21 +1.8 207,293 + Psyr_0196 0.21 +1.5 207,294 - Psyr_0196 0.22 -2.5 207,294 - Psyr_0196 0.22 -2.8 207,308 + Psyr_0196 0.22 +3.0 207,308 + Psyr_0196 0.22 -2.7 207,311 - Psyr_0196 0.22 -1.6 207,371 - Psyr_0196 0.24 +2.5 207,371 - Psyr_0196 0.24 -1.4 207,431 + Psyr_0196 0.26 -0.8 207,448 + Psyr_0196 0.26 -2.2 207,448 + Psyr_0196 0.26 -1.9 207,449 - Psyr_0196 0.26 +2.5 207,449 - Psyr_0196 0.26 -1.6 207,482 + Psyr_0196 0.27 -0.2 207,548 + Psyr_0196 0.29 -1.4 207,548 + Psyr_0196 0.29 +1.1 207,549 - Psyr_0196 0.29 -2.1 207,549 - Psyr_0196 0.29 -2.1 207,680 + Psyr_0196 0.33 -2.7 207,698 - Psyr_0196 0.33 +3.0 207,703 + Psyr_0196 0.34 +3.9 207,703 + Psyr_0196 0.34 -1.2 207,703 + Psyr_0196 0.34 -1.8 207,703 + Psyr_0196 0.34 -0.4 207,703 + Psyr_0196 0.34 +3.8 207,703 + Psyr_0196 0.34 -1.0 207,703 + Psyr_0196 0.34 +2.5 207,703 + Psyr_0196 0.34 +0.4 207,704 - Psyr_0196 0.34 +1.8 207,704 - Psyr_0196 0.34 -0.6 207,704 - Psyr_0196 0.34 -1.8 207,704 - Psyr_0196 0.34 +0.7 207,704 - Psyr_0196 0.34 -0.1 207,704 - Psyr_0196 0.34 +2.2 207,704 - Psyr_0196 0.34 +1.2 207,704 - Psyr_0196 0.34 +4.0 207,704 - Psyr_0196 0.34 -0.1 207,704 - Psyr_0196 0.34 -0.4 207,704 - Psyr_0196 0.34 +0.6 207,717 + Psyr_0196 0.34 -1.5 207,717 + Psyr_0196 0.34 -0.8 207,718 - Psyr_0196 0.34 -1.8 207,718 - Psyr_0196 0.34 -0.3 207,718 - Psyr_0196 0.34 +2.2 207,718 - Psyr_0196 0.34 -1.0 207,718 - Psyr_0196 0.34 +0.9 207,774 + Psyr_0196 0.36 -0.9 207,775 - Psyr_0196 0.36 +1.2 207,943 - Psyr_0196 0.41 -1.4 208,048 - Psyr_0196 0.44 -0.4 208,048 - Psyr_0196 0.44 +0.6 208,048 - Psyr_0196 0.44 -1.3 208,048 - Psyr_0196 0.44 -1.1 208,159 + Psyr_0196 0.47 -0.9 208,168 + Psyr_0196 0.47 +0.5 208,623 + Psyr_0196 0.61 -0.2 208,623 + Psyr_0196 0.61 -0.8 208,623 + Psyr_0196 0.61 -2.2 208,623 + Psyr_0196 0.61 +4.5 208,628 + Psyr_0196 0.61 -1.1 208,628 + Psyr_0196 0.61 -3.9 208,768 - Psyr_0196 0.65 -0.3 208,998 + Psyr_0196 0.72 -1.7 209,302 + Psyr_0196 0.81 +0.0 209,302 + Psyr_0196 0.81 +5.3 209,302 + Psyr_0196 0.81 +0.0 209,302 + Psyr_0196 0.81 +0.5 209,303 - Psyr_0196 0.81 -0.9 209,303 - Psyr_0196 0.81 -1.0 209,303 - Psyr_0196 0.81 +4.0 209,303 - Psyr_0196 0.81 +0.0 209,422 - Psyr_0196 0.84 -3.5 209,655 + +0.2 209,656 - +1.0 209,656 - +3.3 209,656 - -2.7 209,656 - -0.1 209,656 - -3.5 209,656 - -0.8 209,656 - -1.2 209,656 - +3.4 209,656 - -1.8 209,656 - -1.3 209,685 + +1.2 209,685 + -1.9 209,686 - -1.9 209,686 - +2.2 209,686 - +2.2 209,686 - +2.2 209,691 + -1.3 209,691 + +0.6 209,691 + -0.8 209,691 + -1.3 209,691 + -0.5 209,691 + -1.2 209,691 + -1.5 209,691 + -0.3 209,691 + +3.4 209,691 + -0.6 209,691 + -1.2 209,691 + +1.8 209,691 + -3.1 209,691 + +1.8 209,691 + -1.2 209,691 + +2.8 209,691 + +3.8 209,691 + -1.5 209,691 + +0.1 209,691 + +2.2 209,691 + -2.1 209,691 + +1.2 209,691 + -1.5 209,691 + +0.2 209,691 + -0.6 209,691 + -0.4 209,691 + -0.2 209,691 + -1.7 209,691 + -0.8 209,691 + +3.0 209,691 + -0.8 209,691 + -0.1 209,691 + -3.1 209,691 + +3.0 209,691 + +0.6 209,691 + +1.8 209,691 + +0.8 209,691 + -0.3 209,691 + +1.5 209,691 + +0.6 209,691 + -2.4 209,691 + +4.1 209,691 + -0.9 209,691 + -0.3 209,691 + -1.2 209,691 + +0.0 209,698 - -0.7 209,698 - -1.8 209,698 - -0.1 209,706 + +0.3 209,706 + -0.3 209,706 + -1.0 209,706 + +1.8 209,706 + -1.7 209,706 + +0.7 209,707 - +0.2 209,723 - -0.9 209,725 - -0.4 209,725 - -0.5 209,725 - +2.2 209,725 - +1.2 209,725 - -2.8 209,725 - -1.8 209,989 + -0.6 209,990 - +0.2 209,990 - -0.5 210,012 - -0.4 210,047 + -3.1 210,048 - +1.8 210,093 - -2.3 210,152 + +0.3 210,152 + +2.5 210,153 - +1.2 210,153 - +1.8 210,153 - +1.2 210,154 + +0.7 210,154 + +1.2 210,154 + -1.1 210,155 - +3.5 210,155 - -2.9 210,207 + -0.3 210,207 + -1.0 210,207 + -0.6 210,207 + -2.9 210,207 + +0.1 210,207 + +0.8 210,208 - -0.5 210,208 - -0.8 210,208 - +4.1 210,235 + -2.3 210,235 + +0.8 210,236 - +3.2 210,236 - +2.8 210,236 - -0.6 210,271 - -0.4 210,302 + +0.1 210,302 + -0.6 210,303 - -3.5 210,329 + -0.3 210,329 + +0.8 210,329 + +2.3 210,330 - -0.6 210,365 + +2.2 210,917 + Psyr_0197 0.38 -0.4
Or see this region's nucleotide sequence