Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0177 and Psyr_0178 are separated by 131 nucleotides Psyr_0178 and Psyr_0179 are separated by 134 nucleotides Psyr_0179 and Psyr_0180 are separated by 2 nucleotides Psyr_0180 and Psyr_0181 are separated by 238 nucleotides Psyr_0181 and Psyr_0182 are separated by 9 nucleotides
Psyr_0177: Psyr_0177 - adenylate cyclase, at 190,574 to 193,420
_0177
Psyr_0178: Psyr_0178 - regulator of nucleoside diphosphate kinase, at 193,552 to 193,959
_0178
Psyr_0179: Psyr_0179 - Protein of unknown function DUF1289, at 194,094 to 194,330
_0179
Psyr_0180: Psyr_0180 - Frataxin-like protein, at 194,333 to 194,665
_0180
Psyr_0181: Psyr_0181 - lipoprotein LppL, at 194,904 to 195,050
_0181
Psyr_0182: Psyr_0182 - diaminopimelate decarboxylase, at 195,060 to 196,307
_0182
Position (kb) 194
195 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 193.554 kb on - strand at 193.583 kb on + strand at 193.583 kb on + strand at 193.583 kb on + strand at 193.583 kb on + strand at 193.745 kb on + strand, within Psyr_0178 at 193.745 kb on + strand, within Psyr_0178 at 193.745 kb on + strand, within Psyr_0178 at 193.746 kb on - strand, within Psyr_0178 at 193.918 kb on + strand, within Psyr_0178 at 193.918 kb on + strand, within Psyr_0178 at 193.918 kb on + strand, within Psyr_0178 at 193.919 kb on - strand at 193.919 kb on - strand at 193.919 kb on - strand at 193.919 kb on - strand at 193.919 kb on - strand at 193.978 kb on + strand at 193.978 kb on + strand at 193.979 kb on - strand at 193.991 kb on + strand at 193.991 kb on + strand at 193.991 kb on + strand at 193.991 kb on + strand at 193.992 kb on - strand at 193.992 kb on - strand at 193.992 kb on - strand at 194.058 kb on + strand at 194.059 kb on - strand at 194.059 kb on - strand at 194.071 kb on + strand at 194.072 kb on - strand at 194.075 kb on + strand at 194.075 kb on + strand at 194.075 kb on + strand at 194.075 kb on + strand at 194.075 kb on + strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.076 kb on - strand at 194.258 kb on + strand, within Psyr_0179 at 194.258 kb on + strand, within Psyr_0179 at 194.259 kb on - strand, within Psyr_0179 at 194.449 kb on - strand, within Psyr_0180 at 194.518 kb on + strand, within Psyr_0180 at 194.518 kb on + strand, within Psyr_0180 at 194.518 kb on + strand, within Psyr_0180 at 194.518 kb on + strand, within Psyr_0180 at 194.519 kb on - strand, within Psyr_0180 at 194.519 kb on - strand, within Psyr_0180 at 194.519 kb on - strand, within Psyr_0180 at 194.553 kb on - strand, within Psyr_0180 at 194.600 kb on + strand, within Psyr_0180 at 194.600 kb on + strand, within Psyr_0180 at 194.601 kb on - strand, within Psyr_0180 at 194.601 kb on - strand, within Psyr_0180 at 194.601 kb on - strand, within Psyr_0180 at 194.612 kb on - strand, within Psyr_0180 at 194.694 kb on + strand at 194.694 kb on + strand at 194.694 kb on + strand at 194.694 kb on + strand at 194.712 kb on + strand at 194.741 kb on + strand at 194.741 kb on + strand at 194.741 kb on + strand at 194.771 kb on - strand at 194.771 kb on - strand at 194.786 kb on + strand at 194.786 kb on + strand at 194.786 kb on + strand at 194.786 kb on + strand at 194.786 kb on + strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 194.787 kb on - strand at 195.540 kb on + strand, within Psyr_0182
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 193,554 - -0.3 193,583 + +0.4 193,583 + -2.7 193,583 + +3.0 193,583 + -2.6 193,745 + Psyr_0178 0.47 -1.5 193,745 + Psyr_0178 0.47 +0.1 193,745 + Psyr_0178 0.47 -2.1 193,746 - Psyr_0178 0.48 +1.2 193,918 + Psyr_0178 0.90 -0.3 193,918 + Psyr_0178 0.90 +3.5 193,918 + Psyr_0178 0.90 +3.2 193,919 - +4.1 193,919 - +2.8 193,919 - -0.0 193,919 - -0.2 193,919 - +2.2 193,978 + -1.6 193,978 + +4.0 193,979 - +0.1 193,991 + -0.8 193,991 + -1.3 193,991 + +4.3 193,991 + -1.4 193,992 - -0.4 193,992 - +2.2 193,992 - +0.6 194,058 + -3.1 194,059 - -0.4 194,059 - +0.2 194,071 + -1.8 194,072 - +2.2 194,075 + +2.8 194,075 + -0.8 194,075 + -1.7 194,075 + +1.3 194,075 + +3.4 194,076 - -1.0 194,076 - +2.2 194,076 - -1.5 194,076 - +0.0 194,076 - +3.5 194,076 - +3.4 194,076 - +0.2 194,076 - -1.9 194,076 - +3.4 194,076 - +2.2 194,076 - +0.3 194,258 + Psyr_0179 0.69 -3.8 194,258 + Psyr_0179 0.69 -1.1 194,259 - Psyr_0179 0.70 +3.5 194,449 - Psyr_0180 0.35 +3.4 194,518 + Psyr_0180 0.56 +3.2 194,518 + Psyr_0180 0.56 +3.0 194,518 + Psyr_0180 0.56 -1.5 194,518 + Psyr_0180 0.56 +3.0 194,519 - Psyr_0180 0.56 +3.8 194,519 - Psyr_0180 0.56 +2.8 194,519 - Psyr_0180 0.56 +3.0 194,553 - Psyr_0180 0.66 -0.3 194,600 + Psyr_0180 0.80 -2.2 194,600 + Psyr_0180 0.80 +2.6 194,601 - Psyr_0180 0.80 +1.7 194,601 - Psyr_0180 0.80 +1.2 194,601 - Psyr_0180 0.80 +1.8 194,612 - Psyr_0180 0.84 +0.7 194,694 + +1.1 194,694 + -4.8 194,694 + -3.1 194,694 + -2.4 194,712 + -0.6 194,741 + +2.8 194,741 + +3.0 194,741 + -2.5 194,771 - -0.8 194,771 - -0.4 194,786 + -0.4 194,786 + -0.5 194,786 + +1.2 194,786 + +3.7 194,786 + -1.5 194,787 - +1.2 194,787 - +5.1 194,787 - +1.8 194,787 - +0.2 194,787 - +4.0 194,787 - +0.4 194,787 - -2.0 194,787 - +0.3 195,540 + Psyr_0182 0.38 +0.2
Or see this region's nucleotide sequence