Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0171 and Psyr_0172 are separated by 16 nucleotides Psyr_0172 and Psyr_0173 overlap by 4 nucleotides Psyr_0173 and Psyr_0174 are separated by 456 nucleotides
Psyr_0171: Psyr_0171 - EAL, at 185,595 to 186,377
_0171
Psyr_0172: Psyr_0172 - Protein of unknown function DUF785, at 186,394 to 186,870
_0172
Psyr_0173: Psyr_0173 - SSU ribosomal protein S6P modification protein, at 186,867 to 187,772
_0173
Psyr_0174: Psyr_0174 - Sodium:dicarboxylate symporter, at 188,229 to 189,560
_0174
Position (kb) 186
187
188 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 185.921 kb on + strand, within Psyr_0171 at 186.230 kb on + strand, within Psyr_0171 at 186.230 kb on + strand, within Psyr_0171 at 186.231 kb on - strand, within Psyr_0171 at 186.231 kb on - strand, within Psyr_0171 at 186.257 kb on + strand, within Psyr_0171 at 186.257 kb on + strand, within Psyr_0171 at 186.258 kb on - strand, within Psyr_0171 at 186.258 kb on - strand, within Psyr_0171 at 186.258 kb on - strand, within Psyr_0171 at 186.258 kb on - strand, within Psyr_0171 at 186.258 kb on - strand, within Psyr_0171 at 186.258 kb on - strand, within Psyr_0171 at 186.293 kb on - strand, within Psyr_0171 at 186.309 kb on + strand at 186.309 kb on + strand at 186.309 kb on + strand at 186.310 kb on - strand at 186.310 kb on - strand at 186.382 kb on + strand at 186.382 kb on + strand at 186.382 kb on + strand at 186.383 kb on - strand at 186.383 kb on - strand at 186.639 kb on + strand, within Psyr_0172 at 186.639 kb on + strand, within Psyr_0172 at 186.670 kb on + strand, within Psyr_0172 at 186.670 kb on + strand, within Psyr_0172 at 186.670 kb on + strand, within Psyr_0172 at 186.670 kb on + strand, within Psyr_0172 at 186.671 kb on - strand, within Psyr_0172 at 186.671 kb on - strand, within Psyr_0172 at 186.671 kb on - strand, within Psyr_0172 at 186.671 kb on - strand, within Psyr_0172 at 186.706 kb on - strand, within Psyr_0172 at 186.706 kb on - strand, within Psyr_0172 at 186.817 kb on + strand, within Psyr_0172 at 186.817 kb on + strand, within Psyr_0172 at 186.817 kb on + strand, within Psyr_0172 at 186.817 kb on + strand, within Psyr_0172 at 186.817 kb on + strand, within Psyr_0172 at 186.817 kb on + strand, within Psyr_0172 at 186.817 kb on + strand, within Psyr_0172 at 186.817 kb on + strand, within Psyr_0172 at 186.817 kb on + strand, within Psyr_0172 at 186.817 kb on + strand, within Psyr_0172 at 186.818 kb on - strand, within Psyr_0172 at 186.818 kb on - strand, within Psyr_0172 at 186.818 kb on - strand, within Psyr_0172 at 186.818 kb on - strand, within Psyr_0172 at 186.818 kb on - strand, within Psyr_0172 at 186.818 kb on - strand, within Psyr_0172 at 186.818 kb on - strand, within Psyr_0172 at 186.821 kb on + strand, within Psyr_0172 at 186.821 kb on + strand, within Psyr_0172 at 186.821 kb on + strand, within Psyr_0172 at 186.821 kb on + strand, within Psyr_0172 at 186.821 kb on + strand, within Psyr_0172 at 186.821 kb on + strand, within Psyr_0172 at 186.821 kb on + strand, within Psyr_0172 at 186.822 kb on - strand, within Psyr_0172 at 186.822 kb on - strand, within Psyr_0172 at 186.822 kb on - strand, within Psyr_0172 at 186.822 kb on - strand, within Psyr_0172 at 186.822 kb on - strand, within Psyr_0172 at 186.822 kb on - strand, within Psyr_0172 at 186.822 kb on - strand, within Psyr_0172 at 186.849 kb on + strand at 186.849 kb on + strand at 186.849 kb on + strand at 186.849 kb on + strand at 186.849 kb on + strand at 186.850 kb on - strand at 186.903 kb on + strand at 186.903 kb on + strand at 186.903 kb on + strand at 186.904 kb on - strand at 186.904 kb on - strand at 186.904 kb on - strand at 186.978 kb on + strand, within Psyr_0173 at 186.978 kb on + strand, within Psyr_0173 at 186.978 kb on + strand, within Psyr_0173 at 186.978 kb on + strand, within Psyr_0173 at 186.978 kb on + strand, within Psyr_0173 at 186.978 kb on + strand, within Psyr_0173 at 186.978 kb on + strand, within Psyr_0173 at 186.978 kb on + strand, within Psyr_0173 at 186.978 kb on + strand, within Psyr_0173 at 186.979 kb on - strand, within Psyr_0173 at 186.979 kb on - strand, within Psyr_0173 at 186.979 kb on - strand, within Psyr_0173 at 186.979 kb on - strand, within Psyr_0173 at 186.979 kb on - strand, within Psyr_0173 at 186.979 kb on - strand, within Psyr_0173 at 186.979 kb on - strand, within Psyr_0173 at 186.980 kb on + strand, within Psyr_0173 at 186.980 kb on + strand, within Psyr_0173 at 186.980 kb on + strand, within Psyr_0173 at 186.981 kb on - strand, within Psyr_0173 at 186.981 kb on - strand, within Psyr_0173 at 186.981 kb on - strand, within Psyr_0173 at 186.981 kb on - strand, within Psyr_0173 at 186.981 kb on - strand, within Psyr_0173 at 186.981 kb on - strand, within Psyr_0173 at 186.981 kb on - strand, within Psyr_0173 at 187.081 kb on - strand, within Psyr_0173 at 187.081 kb on - strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.401 kb on + strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.402 kb on - strand, within Psyr_0173 at 187.843 kb on - strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.847 kb on + strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.848 kb on - strand at 187.856 kb on + strand at 187.935 kb on + strand at 187.935 kb on + strand at 187.935 kb on + strand at 187.935 kb on + strand at 187.936 kb on - strand at 187.936 kb on - strand at 187.936 kb on - strand at 187.978 kb on + strand at 187.979 kb on - strand at 188.047 kb on + strand at 188.047 kb on + strand at 188.047 kb on + strand at 188.047 kb on + strand at 188.047 kb on + strand at 188.047 kb on + strand at 188.048 kb on - strand at 188.048 kb on - strand at 188.048 kb on - strand at 188.048 kb on - strand at 188.048 kb on - strand at 188.050 kb on - strand at 188.066 kb on + strand at 188.066 kb on + strand at 188.067 kb on - strand at 188.067 kb on - strand at 188.089 kb on + strand at 188.222 kb on + strand at 188.223 kb on - strand at 188.223 kb on - strand at 188.225 kb on + strand at 188.225 kb on + strand at 188.225 kb on + strand at 188.226 kb on - strand at 188.226 kb on - strand at 188.226 kb on - strand at 188.226 kb on - strand at 188.226 kb on - strand at 188.226 kb on - strand at 188.226 kb on - strand at 188.228 kb on + strand at 188.228 kb on + strand at 188.228 kb on + strand at 188.229 kb on - strand at 188.229 kb on - strand at 188.229 kb on - strand at 188.367 kb on - strand, within Psyr_0174 at 188.396 kb on + strand, within Psyr_0174 at 188.465 kb on + strand, within Psyr_0174 at 188.465 kb on + strand, within Psyr_0174 at 188.488 kb on - strand, within Psyr_0174 at 188.488 kb on - strand, within Psyr_0174 at 188.488 kb on - strand, within Psyr_0174 at 188.601 kb on + strand, within Psyr_0174 at 188.602 kb on - strand, within Psyr_0174
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 185,921 + Psyr_0171 0.42 -0.5 186,230 + Psyr_0171 0.81 -0.5 186,230 + Psyr_0171 0.81 -1.3 186,231 - Psyr_0171 0.81 +2.7 186,231 - Psyr_0171 0.81 -1.0 186,257 + Psyr_0171 0.85 -2.0 186,257 + Psyr_0171 0.85 +2.2 186,258 - Psyr_0171 0.85 +3.7 186,258 - Psyr_0171 0.85 -2.3 186,258 - Psyr_0171 0.85 +1.0 186,258 - Psyr_0171 0.85 +2.5 186,258 - Psyr_0171 0.85 -1.0 186,258 - Psyr_0171 0.85 +1.8 186,293 - Psyr_0171 0.89 -1.1 186,309 + +2.5 186,309 + +0.2 186,309 + -1.4 186,310 - -0.7 186,310 - -1.6 186,382 + -0.6 186,382 + +0.7 186,382 + -0.7 186,383 - -0.2 186,383 - +3.7 186,639 + Psyr_0172 0.51 -2.6 186,639 + Psyr_0172 0.51 +0.0 186,670 + Psyr_0172 0.58 +3.2 186,670 + Psyr_0172 0.58 +3.9 186,670 + Psyr_0172 0.58 +4.3 186,670 + Psyr_0172 0.58 +1.8 186,671 - Psyr_0172 0.58 -1.0 186,671 - Psyr_0172 0.58 +3.4 186,671 - Psyr_0172 0.58 -1.7 186,671 - Psyr_0172 0.58 -0.7 186,706 - Psyr_0172 0.65 -1.6 186,706 - Psyr_0172 0.65 +3.2 186,817 + Psyr_0172 0.89 -0.7 186,817 + Psyr_0172 0.89 +3.2 186,817 + Psyr_0172 0.89 -0.5 186,817 + Psyr_0172 0.89 +3.4 186,817 + Psyr_0172 0.89 +0.5 186,817 + Psyr_0172 0.89 -1.6 186,817 + Psyr_0172 0.89 +0.2 186,817 + Psyr_0172 0.89 +0.9 186,817 + Psyr_0172 0.89 +4.5 186,817 + Psyr_0172 0.89 -0.1 186,818 - Psyr_0172 0.89 +1.1 186,818 - Psyr_0172 0.89 +0.8 186,818 - Psyr_0172 0.89 -3.2 186,818 - Psyr_0172 0.89 +1.2 186,818 - Psyr_0172 0.89 +0.8 186,818 - Psyr_0172 0.89 -0.1 186,818 - Psyr_0172 0.89 -0.2 186,821 + Psyr_0172 0.90 -2.0 186,821 + Psyr_0172 0.90 +3.9 186,821 + Psyr_0172 0.90 +0.2 186,821 + Psyr_0172 0.90 +0.0 186,821 + Psyr_0172 0.90 -1.2 186,821 + Psyr_0172 0.90 -1.1 186,821 + Psyr_0172 0.90 -0.9 186,822 - Psyr_0172 0.90 +1.2 186,822 - Psyr_0172 0.90 +1.2 186,822 - Psyr_0172 0.90 +2.5 186,822 - Psyr_0172 0.90 -0.3 186,822 - Psyr_0172 0.90 +0.2 186,822 - Psyr_0172 0.90 +3.0 186,822 - Psyr_0172 0.90 -1.0 186,849 + -0.7 186,849 + -1.2 186,849 + -0.4 186,849 + -0.2 186,849 + -0.9 186,850 - -3.6 186,903 + -1.5 186,903 + -2.3 186,903 + +2.5 186,904 - -0.9 186,904 - -2.7 186,904 - +0.1 186,978 + Psyr_0173 0.12 -1.2 186,978 + Psyr_0173 0.12 +0.4 186,978 + Psyr_0173 0.12 +4.0 186,978 + Psyr_0173 0.12 +3.2 186,978 + Psyr_0173 0.12 +3.2 186,978 + Psyr_0173 0.12 -0.6 186,978 + Psyr_0173 0.12 -0.1 186,978 + Psyr_0173 0.12 -0.2 186,978 + Psyr_0173 0.12 +0.1 186,979 - Psyr_0173 0.12 +3.9 186,979 - Psyr_0173 0.12 +3.4 186,979 - Psyr_0173 0.12 +3.8 186,979 - Psyr_0173 0.12 +0.2 186,979 - Psyr_0173 0.12 -1.5 186,979 - Psyr_0173 0.12 +3.5 186,979 - Psyr_0173 0.12 +1.5 186,980 + Psyr_0173 0.12 +0.5 186,980 + Psyr_0173 0.12 -1.4 186,980 + Psyr_0173 0.12 +0.7 186,981 - Psyr_0173 0.13 +2.6 186,981 - Psyr_0173 0.13 -0.5 186,981 - Psyr_0173 0.13 +1.2 186,981 - Psyr_0173 0.13 +1.2 186,981 - Psyr_0173 0.13 +1.2 186,981 - Psyr_0173 0.13 +0.7 186,981 - Psyr_0173 0.13 +0.3 187,081 - Psyr_0173 0.24 +3.5 187,081 - Psyr_0173 0.24 +4.4 187,401 + Psyr_0173 0.59 +3.2 187,401 + Psyr_0173 0.59 -2.0 187,401 + Psyr_0173 0.59 -0.5 187,401 + Psyr_0173 0.59 -1.1 187,401 + Psyr_0173 0.59 -4.7 187,401 + Psyr_0173 0.59 +0.2 187,401 + Psyr_0173 0.59 +3.0 187,401 + Psyr_0173 0.59 +3.9 187,401 + Psyr_0173 0.59 +3.2 187,401 + Psyr_0173 0.59 -1.4 187,402 - Psyr_0173 0.59 +2.5 187,402 - Psyr_0173 0.59 -2.5 187,402 - Psyr_0173 0.59 -3.8 187,402 - Psyr_0173 0.59 +2.5 187,402 - Psyr_0173 0.59 -0.6 187,402 - Psyr_0173 0.59 -0.4 187,402 - Psyr_0173 0.59 +0.2 187,402 - Psyr_0173 0.59 -0.2 187,843 - +1.2 187,847 + +1.8 187,847 + -1.9 187,847 + -0.2 187,847 + -3.2 187,847 + -2.2 187,847 + -0.8 187,847 + -1.8 187,847 + +0.2 187,847 + +0.2 187,847 + +0.5 187,847 + -1.6 187,848 - +1.2 187,848 - -0.8 187,848 - +1.8 187,848 - -1.9 187,848 - +3.7 187,848 - -1.2 187,848 - +3.0 187,848 - -0.7 187,848 - -1.5 187,848 - -0.1 187,856 + -1.2 187,935 + -2.4 187,935 + +4.0 187,935 + +2.5 187,935 + -0.9 187,936 - -0.1 187,936 - +2.8 187,936 - -4.3 187,978 + +0.3 187,979 - +3.9 188,047 + -0.5 188,047 + -1.2 188,047 + -0.6 188,047 + +0.9 188,047 + +3.2 188,047 + +1.3 188,048 - +1.0 188,048 - +2.5 188,048 - -1.4 188,048 - +2.2 188,048 - -0.7 188,050 - -1.5 188,066 + +4.7 188,066 + +0.7 188,067 - +0.2 188,067 - -2.0 188,089 + +2.5 188,222 + -1.0 188,223 - -0.5 188,223 - +3.2 188,225 + -1.1 188,225 + -1.6 188,225 + -1.5 188,226 - +2.5 188,226 - +3.5 188,226 - -1.6 188,226 - +0.5 188,226 - -0.7 188,226 - -0.1 188,226 - +0.2 188,228 + +0.3 188,228 + +1.8 188,228 + -0.3 188,229 - -0.4 188,229 - -4.0 188,229 - -0.8 188,367 - Psyr_0174 0.10 +3.5 188,396 + Psyr_0174 0.13 -2.0 188,465 + Psyr_0174 0.18 -2.3 188,465 + Psyr_0174 0.18 +1.8 188,488 - Psyr_0174 0.19 -2.1 188,488 - Psyr_0174 0.19 -0.7 188,488 - Psyr_0174 0.19 -2.0 188,601 + Psyr_0174 0.28 +0.2 188,602 - Psyr_0174 0.28 +3.0
Or see this region's nucleotide sequence