Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0166 and Psyr_0167 are separated by 5 nucleotides Psyr_0167 and Psyr_0168 are separated by 370 nucleotides
Psyr_0166: Psyr_0166 - hypothetical protein, at 180,107 to 181,417
_0166
Psyr_0167: Psyr_0167 - hypothetical protein, at 181,423 to 181,845
_0167
Psyr_0168: Psyr_0168 - Phosphoenolpyruvate carboxykinase (ATP), at 182,216 to 183,760
_0168
Position (kb)
181
182 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 180.473 kb on - strand, within Psyr_0166 at 180.529 kb on + strand, within Psyr_0166 at 180.529 kb on + strand, within Psyr_0166 at 180.533 kb on + strand, within Psyr_0166 at 180.533 kb on + strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.722 kb on - strand, within Psyr_0166 at 180.748 kb on + strand, within Psyr_0166 at 180.847 kb on - strand, within Psyr_0166 at 180.847 kb on - strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.996 kb on - strand, within Psyr_0166 at 180.996 kb on - strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 181.012 kb on + strand, within Psyr_0166 at 181.016 kb on + strand, within Psyr_0166 at 181.017 kb on - strand, within Psyr_0166 at 181.042 kb on + strand, within Psyr_0166 at 181.042 kb on + strand, within Psyr_0166 at 181.092 kb on - strand, within Psyr_0166 at 181.151 kb on - strand, within Psyr_0166 at 181.184 kb on + strand, within Psyr_0166 at 181.274 kb on + strand, within Psyr_0166 at 181.286 kb on + strand, within Psyr_0166 at 181.287 kb on - strand at 181.288 kb on + strand at 181.289 kb on - strand at 181.328 kb on - strand at 181.387 kb on + strand at 181.611 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.694 kb on - strand, within Psyr_0167 at 181.694 kb on - strand, within Psyr_0167 at 181.696 kb on - strand, within Psyr_0167 at 181.798 kb on - strand, within Psyr_0167 at 181.843 kb on + strand at 181.847 kb on + strand at 181.847 kb on + strand at 181.847 kb on + strand at 181.848 kb on - strand at 181.848 kb on - strand at 181.904 kb on + strand at 181.904 kb on + strand at 181.904 kb on + strand at 181.905 kb on - strand at 182.034 kb on - strand at 182.054 kb on - strand at 182.084 kb on + strand at 182.086 kb on + strand at 182.087 kb on - strand at 182.087 kb on - strand at 182.104 kb on - strand at 182.128 kb on - strand at 182.207 kb on + strand at 182.207 kb on + strand at 182.208 kb on - strand at 182.217 kb on + strand at 182.218 kb on - strand at 182.218 kb on - strand at 182.313 kb on + strand at 182.314 kb on - strand at 182.532 kb on + strand, within Psyr_0168 at 182.532 kb on + strand, within Psyr_0168 at 182.532 kb on + strand, within Psyr_0168 at 182.532 kb on + strand, within Psyr_0168 at 182.532 kb on + strand, within Psyr_0168 at 182.533 kb on - strand, within Psyr_0168 at 182.533 kb on - strand, within Psyr_0168 at 182.744 kb on + strand, within Psyr_0168 at 182.835 kb on + strand, within Psyr_0168
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 180,473 - Psyr_0166 0.28 +1.2 180,529 + Psyr_0166 0.32 +0.2 180,529 + Psyr_0166 0.32 -0.1 180,533 + Psyr_0166 0.32 -1.7 180,533 + Psyr_0166 0.32 +0.2 180,534 - Psyr_0166 0.33 -2.7 180,534 - Psyr_0166 0.33 +4.2 180,534 - Psyr_0166 0.33 +1.2 180,534 - Psyr_0166 0.33 +2.5 180,534 - Psyr_0166 0.33 +2.5 180,534 - Psyr_0166 0.33 -0.5 180,534 - Psyr_0166 0.33 -1.2 180,722 - Psyr_0166 0.47 +3.5 180,748 + Psyr_0166 0.49 +4.7 180,847 - Psyr_0166 0.56 -2.5 180,847 - Psyr_0166 0.56 -0.3 180,995 + Psyr_0166 0.68 +1.2 180,995 + Psyr_0166 0.68 -0.7 180,995 + Psyr_0166 0.68 -5.1 180,995 + Psyr_0166 0.68 -0.2 180,996 - Psyr_0166 0.68 +1.2 180,996 - Psyr_0166 0.68 +0.2 180,997 + Psyr_0166 0.68 +5.0 180,997 + Psyr_0166 0.68 -0.2 180,997 + Psyr_0166 0.68 -1.7 180,997 + Psyr_0166 0.68 -1.0 181,012 + Psyr_0166 0.69 +0.6 181,016 + Psyr_0166 0.69 -0.8 181,017 - Psyr_0166 0.69 -1.6 181,042 + Psyr_0166 0.71 -0.3 181,042 + Psyr_0166 0.71 +4.7 181,092 - Psyr_0166 0.75 +0.2 181,151 - Psyr_0166 0.80 -3.8 181,184 + Psyr_0166 0.82 +0.5 181,274 + Psyr_0166 0.89 +3.5 181,286 + Psyr_0166 0.90 -0.7 181,287 - +0.2 181,288 + -1.6 181,289 - -0.8 181,328 - +2.0 181,387 + -2.1 181,611 + Psyr_0167 0.44 -0.4 181,693 + Psyr_0167 0.64 +0.2 181,693 + Psyr_0167 0.64 -1.1 181,693 + Psyr_0167 0.64 -1.9 181,693 + Psyr_0167 0.64 +0.2 181,694 - Psyr_0167 0.64 +2.2 181,694 - Psyr_0167 0.64 -2.5 181,696 - Psyr_0167 0.65 -2.0 181,798 - Psyr_0167 0.89 +2.2 181,843 + +1.8 181,847 + +3.5 181,847 + -1.4 181,847 + -1.8 181,848 - -1.7 181,848 - -3.6 181,904 + -1.3 181,904 + -1.7 181,904 + +0.2 181,905 - +0.2 182,034 - -1.1 182,054 - -0.5 182,084 + -0.1 182,086 + +1.8 182,087 - +2.5 182,087 - -1.0 182,104 - -2.5 182,128 - -4.0 182,207 + -0.1 182,207 + -0.9 182,208 - +2.5 182,217 + -1.1 182,218 - +0.7 182,218 - -1.3 182,313 + -1.5 182,314 - -1.5 182,532 + Psyr_0168 0.20 -3.1 182,532 + Psyr_0168 0.20 -0.9 182,532 + Psyr_0168 0.20 +2.5 182,532 + Psyr_0168 0.20 -1.6 182,532 + Psyr_0168 0.20 +4.3 182,533 - Psyr_0168 0.21 +3.4 182,533 - Psyr_0168 0.21 +2.5 182,744 + Psyr_0168 0.34 -0.8 182,835 + Psyr_0168 0.40 +4.3
Or see this region's nucleotide sequence