Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0161 and Psyr_0162 are separated by 441 nucleotides Psyr_0162 and Psyr_0163 are separated by 130 nucleotides Psyr_0163 and Psyr_0164 are separated by 9 nucleotides
Psyr_0161: Psyr_0161 - Protein of unknown function DUF861, at 175,914 to 176,627
_0161
Psyr_0162: Psyr_0162 - hypothetical protein, at 177,069 to 177,518
_0162
Psyr_0163: Psyr_0163 - conserved hypothetical protein, at 177,649 to 178,356
_0163
Psyr_0164: Psyr_0164 - conserved hypothetical protein, at 178,366 to 179,082
_0164
Position (kb) 177
178 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 176.097 kb on + strand, within Psyr_0161 at 176.104 kb on - strand, within Psyr_0161 at 176.104 kb on - strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.212 kb on + strand, within Psyr_0161 at 176.213 kb on - strand, within Psyr_0161 at 176.213 kb on - strand, within Psyr_0161 at 176.213 kb on - strand, within Psyr_0161 at 176.213 kb on - strand, within Psyr_0161 at 176.213 kb on - strand, within Psyr_0161 at 176.213 kb on - strand, within Psyr_0161 at 176.216 kb on + strand, within Psyr_0161 at 176.217 kb on - strand, within Psyr_0161 at 176.217 kb on - strand, within Psyr_0161 at 176.598 kb on + strand at 176.598 kb on + strand at 176.598 kb on + strand at 176.598 kb on + strand at 176.598 kb on + strand at 176.598 kb on + strand at 176.598 kb on + strand at 176.599 kb on - strand at 176.599 kb on - strand at 176.614 kb on - strand at 176.614 kb on - strand at 176.636 kb on + strand at 176.683 kb on + strand at 176.683 kb on + strand at 176.684 kb on - strand at 176.684 kb on - strand at 176.684 kb on - strand at 176.721 kb on - strand at 176.802 kb on - strand at 176.873 kb on + strand at 176.906 kb on + strand at 176.906 kb on + strand at 176.907 kb on - strand at 176.907 kb on - strand at 176.907 kb on - strand at 176.907 kb on - strand at 176.946 kb on + strand at 176.946 kb on + strand at 176.946 kb on + strand at 176.946 kb on + strand at 176.947 kb on - strand at 176.947 kb on - strand at 176.947 kb on - strand at 176.947 kb on - strand at 176.988 kb on + strand at 177.046 kb on - strand at 177.049 kb on + strand at 177.194 kb on + strand, within Psyr_0162 at 177.194 kb on + strand, within Psyr_0162 at 177.194 kb on + strand, within Psyr_0162 at 177.194 kb on + strand, within Psyr_0162 at 177.194 kb on + strand, within Psyr_0162 at 177.194 kb on + strand, within Psyr_0162 at 177.195 kb on - strand, within Psyr_0162 at 177.195 kb on - strand, within Psyr_0162 at 177.282 kb on + strand, within Psyr_0162 at 177.282 kb on + strand, within Psyr_0162 at 177.283 kb on - strand, within Psyr_0162 at 177.283 kb on - strand, within Psyr_0162 at 177.283 kb on - strand, within Psyr_0162 at 177.284 kb on + strand, within Psyr_0162 at 177.285 kb on - strand, within Psyr_0162 at 177.285 kb on - strand, within Psyr_0162 at 177.318 kb on + strand, within Psyr_0162 at 177.318 kb on + strand, within Psyr_0162 at 177.319 kb on - strand, within Psyr_0162 at 177.334 kb on - strand, within Psyr_0162 at 177.334 kb on - strand, within Psyr_0162 at 177.352 kb on + strand, within Psyr_0162 at 177.352 kb on + strand, within Psyr_0162 at 177.352 kb on + strand, within Psyr_0162 at 177.352 kb on + strand, within Psyr_0162 at 177.352 kb on + strand, within Psyr_0162 at 177.352 kb on + strand, within Psyr_0162 at 177.352 kb on + strand, within Psyr_0162 at 177.352 kb on + strand, within Psyr_0162 at 177.352 kb on + strand, within Psyr_0162 at 177.352 kb on + strand, within Psyr_0162 at 177.352 kb on + strand, within Psyr_0162 at 177.352 kb on + strand, within Psyr_0162 at 177.353 kb on - strand, within Psyr_0162 at 177.353 kb on - strand, within Psyr_0162 at 177.353 kb on - strand, within Psyr_0162 at 177.353 kb on - strand, within Psyr_0162 at 177.353 kb on - strand, within Psyr_0162 at 177.377 kb on - strand, within Psyr_0162 at 177.377 kb on - strand, within Psyr_0162 at 177.422 kb on + strand, within Psyr_0162 at 177.458 kb on + strand, within Psyr_0162 at 177.458 kb on + strand, within Psyr_0162 at 177.458 kb on + strand, within Psyr_0162 at 177.459 kb on - strand, within Psyr_0162 at 177.459 kb on - strand, within Psyr_0162 at 177.459 kb on - strand, within Psyr_0162 at 177.459 kb on - strand, within Psyr_0162 at 177.459 kb on - strand, within Psyr_0162 at 177.483 kb on - strand at 177.516 kb on + strand at 177.516 kb on + strand at 177.537 kb on - strand at 177.544 kb on + strand at 177.545 kb on - strand at 177.573 kb on + strand at 177.573 kb on + strand at 177.574 kb on - strand at 177.599 kb on + strand at 177.599 kb on + strand at 177.599 kb on + strand at 177.599 kb on + strand at 177.599 kb on + strand at 177.599 kb on + strand at 177.599 kb on + strand at 177.599 kb on + strand at 177.599 kb on + strand at 177.599 kb on + strand at 177.600 kb on - strand at 177.600 kb on - strand at 177.600 kb on - strand at 177.600 kb on - strand at 177.600 kb on - strand at 177.600 kb on - strand at 177.604 kb on - strand at 177.610 kb on + strand at 177.610 kb on + strand at 177.611 kb on - strand at 177.611 kb on - strand at 177.678 kb on + strand at 177.678 kb on + strand at 177.678 kb on + strand at 177.679 kb on - strand at 177.696 kb on - strand at 177.743 kb on + strand, within Psyr_0163 at 177.743 kb on + strand, within Psyr_0163 at 177.743 kb on + strand, within Psyr_0163 at 177.744 kb on - strand, within Psyr_0163 at 177.744 kb on - strand, within Psyr_0163 at 177.744 kb on - strand, within Psyr_0163 at 177.744 kb on - strand, within Psyr_0163 at 177.747 kb on + strand, within Psyr_0163 at 177.747 kb on + strand, within Psyr_0163 at 177.747 kb on + strand, within Psyr_0163 at 177.747 kb on + strand, within Psyr_0163 at 177.747 kb on + strand, within Psyr_0163 at 177.748 kb on - strand, within Psyr_0163 at 177.748 kb on - strand, within Psyr_0163 at 177.748 kb on - strand, within Psyr_0163 at 177.748 kb on - strand, within Psyr_0163 at 177.748 kb on - strand, within Psyr_0163 at 177.775 kb on - strand, within Psyr_0163 at 177.840 kb on + strand, within Psyr_0163 at 177.848 kb on + strand, within Psyr_0163 at 177.910 kb on + strand, within Psyr_0163 at 177.910 kb on + strand, within Psyr_0163 at 177.910 kb on + strand, within Psyr_0163 at 177.910 kb on + strand, within Psyr_0163 at 177.910 kb on + strand, within Psyr_0163 at 177.911 kb on - strand, within Psyr_0163 at 177.911 kb on - strand, within Psyr_0163 at 177.911 kb on - strand, within Psyr_0163 at 177.911 kb on - strand, within Psyr_0163 at 177.911 kb on - strand, within Psyr_0163 at 177.913 kb on + strand, within Psyr_0163 at 178.063 kb on + strand, within Psyr_0163 at 178.063 kb on + strand, within Psyr_0163 at 178.063 kb on + strand, within Psyr_0163 at 178.063 kb on + strand, within Psyr_0163 at 178.063 kb on + strand, within Psyr_0163 at 178.063 kb on + strand, within Psyr_0163 at 178.064 kb on - strand, within Psyr_0163 at 178.064 kb on - strand, within Psyr_0163 at 178.064 kb on - strand, within Psyr_0163 at 178.064 kb on - strand, within Psyr_0163 at 178.064 kb on - strand, within Psyr_0163 at 178.089 kb on + strand, within Psyr_0163 at 178.099 kb on - strand, within Psyr_0163 at 178.099 kb on - strand, within Psyr_0163 at 178.143 kb on + strand, within Psyr_0163 at 178.162 kb on + strand, within Psyr_0163 at 178.162 kb on + strand, within Psyr_0163 at 178.163 kb on - strand, within Psyr_0163 at 178.163 kb on - strand, within Psyr_0163 at 178.163 kb on - strand, within Psyr_0163 at 178.174 kb on + strand, within Psyr_0163 at 178.175 kb on - strand, within Psyr_0163 at 178.271 kb on - strand, within Psyr_0163 at 178.284 kb on + strand, within Psyr_0163 at 178.284 kb on + strand, within Psyr_0163 at 178.284 kb on + strand, within Psyr_0163 at 178.284 kb on + strand, within Psyr_0163 at 178.284 kb on + strand, within Psyr_0163 at 178.284 kb on + strand, within Psyr_0163 at 178.284 kb on + strand, within Psyr_0163 at 178.284 kb on + strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.285 kb on - strand, within Psyr_0163 at 178.365 kb on + strand at 178.365 kb on + strand at 178.365 kb on + strand at 178.365 kb on + strand at 178.365 kb on + strand at 178.365 kb on + strand at 178.366 kb on - strand at 178.366 kb on - strand at 178.366 kb on - strand at 178.366 kb on - strand at 178.366 kb on - strand at 178.366 kb on - strand at 178.366 kb on - strand at 178.366 kb on - strand at 178.366 kb on - strand at 178.366 kb on - strand at 178.366 kb on - strand at 178.366 kb on - strand at 178.380 kb on + strand at 178.380 kb on + strand at 178.381 kb on - strand at 178.381 kb on - strand at 178.381 kb on - strand at 178.381 kb on - strand at 178.475 kb on + strand, within Psyr_0164 at 178.475 kb on + strand, within Psyr_0164 at 178.476 kb on - strand, within Psyr_0164 at 178.476 kb on - strand, within Psyr_0164 at 178.476 kb on - strand, within Psyr_0164 at 178.476 kb on - strand, within Psyr_0164 at 178.476 kb on - strand, within Psyr_0164
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 176,097 + Psyr_0161 0.26 -0.9 176,104 - Psyr_0161 0.27 +0.3 176,104 - Psyr_0161 0.27 -1.5 176,212 + Psyr_0161 0.42 +0.1 176,212 + Psyr_0161 0.42 +2.5 176,212 + Psyr_0161 0.42 +3.8 176,212 + Psyr_0161 0.42 +0.2 176,212 + Psyr_0161 0.42 -1.7 176,212 + Psyr_0161 0.42 +3.8 176,212 + Psyr_0161 0.42 -1.7 176,212 + Psyr_0161 0.42 +0.4 176,212 + Psyr_0161 0.42 +3.9 176,212 + Psyr_0161 0.42 +0.3 176,212 + Psyr_0161 0.42 +3.4 176,212 + Psyr_0161 0.42 +2.2 176,212 + Psyr_0161 0.42 +0.2 176,213 - Psyr_0161 0.42 -0.4 176,213 - Psyr_0161 0.42 +0.3 176,213 - Psyr_0161 0.42 +3.2 176,213 - Psyr_0161 0.42 +2.2 176,213 - Psyr_0161 0.42 +2.8 176,213 - Psyr_0161 0.42 +0.9 176,216 + Psyr_0161 0.42 +3.2 176,217 - Psyr_0161 0.42 +0.0 176,217 - Psyr_0161 0.42 -0.1 176,598 + -1.8 176,598 + +3.0 176,598 + -1.1 176,598 + -1.1 176,598 + +0.6 176,598 + +0.3 176,598 + +1.8 176,599 - +3.0 176,599 - +0.4 176,614 - -1.5 176,614 - -0.8 176,636 + +3.7 176,683 + +0.2 176,683 + -3.2 176,684 - -0.8 176,684 - +4.2 176,684 - +0.8 176,721 - -1.6 176,802 - -0.3 176,873 + +0.2 176,906 + +3.2 176,906 + -0.9 176,907 - -0.4 176,907 - -1.6 176,907 - -0.3 176,907 - -3.8 176,946 + -0.8 176,946 + -3.5 176,946 + -0.4 176,946 + +2.5 176,947 - -0.6 176,947 - -1.2 176,947 - -0.3 176,947 - +3.4 176,988 + -1.8 177,046 - +1.8 177,049 + -0.1 177,194 + Psyr_0162 0.28 -0.4 177,194 + Psyr_0162 0.28 -1.0 177,194 + Psyr_0162 0.28 -3.6 177,194 + Psyr_0162 0.28 +4.3 177,194 + Psyr_0162 0.28 +3.5 177,194 + Psyr_0162 0.28 +3.7 177,195 - Psyr_0162 0.28 +0.9 177,195 - Psyr_0162 0.28 -0.1 177,282 + Psyr_0162 0.47 +2.5 177,282 + Psyr_0162 0.47 -0.4 177,283 - Psyr_0162 0.48 +1.8 177,283 - Psyr_0162 0.48 -0.7 177,283 - Psyr_0162 0.48 -0.3 177,284 + Psyr_0162 0.48 -1.1 177,285 - Psyr_0162 0.48 -0.9 177,285 - Psyr_0162 0.48 +1.8 177,318 + Psyr_0162 0.55 -0.9 177,318 + Psyr_0162 0.55 +1.8 177,319 - Psyr_0162 0.56 -1.8 177,334 - Psyr_0162 0.59 +2.2 177,334 - Psyr_0162 0.59 +3.4 177,352 + Psyr_0162 0.63 -0.8 177,352 + Psyr_0162 0.63 -0.3 177,352 + Psyr_0162 0.63 +0.9 177,352 + Psyr_0162 0.63 +0.2 177,352 + Psyr_0162 0.63 +1.8 177,352 + Psyr_0162 0.63 -0.1 177,352 + Psyr_0162 0.63 +4.4 177,352 + Psyr_0162 0.63 +2.2 177,352 + Psyr_0162 0.63 +3.7 177,352 + Psyr_0162 0.63 -0.1 177,352 + Psyr_0162 0.63 +3.9 177,352 + Psyr_0162 0.63 +1.8 177,353 - Psyr_0162 0.63 -1.6 177,353 - Psyr_0162 0.63 +0.2 177,353 - Psyr_0162 0.63 +1.2 177,353 - Psyr_0162 0.63 -0.7 177,353 - Psyr_0162 0.63 +2.2 177,377 - Psyr_0162 0.68 +1.0 177,377 - Psyr_0162 0.68 -3.0 177,422 + Psyr_0162 0.78 +0.7 177,458 + Psyr_0162 0.86 -1.1 177,458 + Psyr_0162 0.86 +1.3 177,458 + Psyr_0162 0.86 -0.7 177,459 - Psyr_0162 0.87 -3.0 177,459 - Psyr_0162 0.87 +2.2 177,459 - Psyr_0162 0.87 -0.5 177,459 - Psyr_0162 0.87 -2.0 177,459 - Psyr_0162 0.87 -1.4 177,483 - -1.8 177,516 + -1.1 177,516 + -1.6 177,537 - +3.0 177,544 + -1.1 177,545 - +3.8 177,573 + -0.6 177,573 + -0.9 177,574 - -1.2 177,599 + -2.0 177,599 + -1.7 177,599 + +0.8 177,599 + +0.8 177,599 + -2.1 177,599 + -1.2 177,599 + -0.3 177,599 + +2.5 177,599 + +2.5 177,599 + -0.2 177,600 - -2.1 177,600 - -1.8 177,600 - -0.6 177,600 - -0.3 177,600 - -0.5 177,600 - +1.2 177,604 - +1.1 177,610 + +4.7 177,610 + -1.7 177,611 - -2.4 177,611 - -0.2 177,678 + +0.2 177,678 + +0.5 177,678 + -0.5 177,679 - -1.5 177,696 - +0.2 177,743 + Psyr_0163 0.13 -1.4 177,743 + Psyr_0163 0.13 +2.8 177,743 + Psyr_0163 0.13 +3.9 177,744 - Psyr_0163 0.13 -0.8 177,744 - Psyr_0163 0.13 +0.3 177,744 - Psyr_0163 0.13 -2.5 177,744 - Psyr_0163 0.13 +0.2 177,747 + Psyr_0163 0.14 -0.4 177,747 + Psyr_0163 0.14 -1.6 177,747 + Psyr_0163 0.14 -1.4 177,747 + Psyr_0163 0.14 -0.9 177,747 + Psyr_0163 0.14 +3.7 177,748 - Psyr_0163 0.14 -0.4 177,748 - Psyr_0163 0.14 +0.7 177,748 - Psyr_0163 0.14 +2.2 177,748 - Psyr_0163 0.14 +1.8 177,748 - Psyr_0163 0.14 +2.2 177,775 - Psyr_0163 0.18 -0.5 177,840 + Psyr_0163 0.27 -1.7 177,848 + Psyr_0163 0.28 -1.6 177,910 + Psyr_0163 0.37 -0.5 177,910 + Psyr_0163 0.37 -0.1 177,910 + Psyr_0163 0.37 -0.7 177,910 + Psyr_0163 0.37 +3.8 177,910 + Psyr_0163 0.37 -1.7 177,911 - Psyr_0163 0.37 -4.1 177,911 - Psyr_0163 0.37 +3.8 177,911 - Psyr_0163 0.37 -0.0 177,911 - Psyr_0163 0.37 +0.4 177,911 - Psyr_0163 0.37 -1.1 177,913 + Psyr_0163 0.37 -0.4 178,063 + Psyr_0163 0.58 +3.4 178,063 + Psyr_0163 0.58 +0.5 178,063 + Psyr_0163 0.58 +0.2 178,063 + Psyr_0163 0.58 +3.0 178,063 + Psyr_0163 0.58 +0.2 178,063 + Psyr_0163 0.58 +1.2 178,064 - Psyr_0163 0.59 +2.2 178,064 - Psyr_0163 0.59 +2.2 178,064 - Psyr_0163 0.59 -0.6 178,064 - Psyr_0163 0.59 +3.2 178,064 - Psyr_0163 0.59 -1.3 178,089 + Psyr_0163 0.62 +0.0 178,099 - Psyr_0163 0.64 -0.8 178,099 - Psyr_0163 0.64 +3.5 178,143 + Psyr_0163 0.70 -2.1 178,162 + Psyr_0163 0.72 +2.8 178,162 + Psyr_0163 0.72 +0.2 178,163 - Psyr_0163 0.73 +0.2 178,163 - Psyr_0163 0.73 +1.2 178,163 - Psyr_0163 0.73 +1.2 178,174 + Psyr_0163 0.74 -0.9 178,175 - Psyr_0163 0.74 +2.2 178,271 - Psyr_0163 0.88 -1.0 178,284 + Psyr_0163 0.90 +3.9 178,284 + Psyr_0163 0.90 +2.8 178,284 + Psyr_0163 0.90 -0.4 178,284 + Psyr_0163 0.90 -2.0 178,284 + Psyr_0163 0.90 +0.6 178,284 + Psyr_0163 0.90 +0.5 178,284 + Psyr_0163 0.90 +2.8 178,284 + Psyr_0163 0.90 -0.8 178,285 - Psyr_0163 0.90 -0.3 178,285 - Psyr_0163 0.90 +2.8 178,285 - Psyr_0163 0.90 +3.0 178,285 - Psyr_0163 0.90 -2.4 178,285 - Psyr_0163 0.90 +4.0 178,285 - Psyr_0163 0.90 -0.9 178,285 - Psyr_0163 0.90 -1.1 178,285 - Psyr_0163 0.90 -0.0 178,285 - Psyr_0163 0.90 +3.1 178,285 - Psyr_0163 0.90 -0.8 178,285 - Psyr_0163 0.90 +2.8 178,285 - Psyr_0163 0.90 +0.4 178,285 - Psyr_0163 0.90 +2.2 178,365 + -0.7 178,365 + +4.1 178,365 + -2.1 178,365 + -3.1 178,365 + -2.3 178,365 + +0.4 178,366 - +1.8 178,366 - -1.2 178,366 - +1.9 178,366 - +0.4 178,366 - +2.5 178,366 - -0.7 178,366 - -2.7 178,366 - +0.2 178,366 - -0.2 178,366 - -1.7 178,366 - -0.2 178,366 - +1.3 178,380 + +2.2 178,380 + +0.9 178,381 - -1.1 178,381 - +1.8 178,381 - -1.4 178,381 - +2.8 178,475 + Psyr_0164 0.15 +2.5 178,475 + Psyr_0164 0.15 +4.8 178,476 - Psyr_0164 0.15 +0.2 178,476 - Psyr_0164 0.15 +2.8 178,476 - Psyr_0164 0.15 -0.4 178,476 - Psyr_0164 0.15 +0.7 178,476 - Psyr_0164 0.15 -1.0
Or see this region's nucleotide sequence