Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0144 and Psyr_0145 are separated by 47 nucleotides Psyr_0145 and Psyr_0146 are separated by 63 nucleotides Psyr_0146 and Psyr_0147 are separated by 284 nucleotides
Psyr_0144: Psyr_0144 - transcriptional regulator, AsnC family, at 157,030 to 157,518
_0144
Psyr_0145: Psyr_0145 - conserved hypothetical protein, at 157,566 to 158,240
_0145
Psyr_0146: Psyr_0146 - 4-aminobutyrate aminotransferase apoenzyme, at 158,304 to 159,608
_0146
Psyr_0147: Psyr_0147 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 159,893 to 160,822
_0147
Position (kb) 158
159
160 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 157.338 kb on + strand, within Psyr_0144 at 157.338 kb on + strand, within Psyr_0144 at 157.339 kb on - strand, within Psyr_0144 at 157.339 kb on - strand, within Psyr_0144 at 157.339 kb on - strand, within Psyr_0144 at 157.339 kb on - strand, within Psyr_0144 at 157.352 kb on + strand, within Psyr_0144 at 157.352 kb on + strand, within Psyr_0144 at 157.352 kb on + strand, within Psyr_0144 at 157.352 kb on + strand, within Psyr_0144 at 157.353 kb on - strand, within Psyr_0144 at 157.353 kb on - strand, within Psyr_0144 at 157.353 kb on - strand, within Psyr_0144 at 157.353 kb on - strand, within Psyr_0144 at 157.364 kb on + strand, within Psyr_0144 at 157.365 kb on - strand, within Psyr_0144 at 157.471 kb on + strand at 157.472 kb on - strand at 157.479 kb on + strand at 157.480 kb on - strand at 157.482 kb on - strand at 157.486 kb on - strand at 157.519 kb on + strand at 157.519 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.524 kb on - strand at 157.524 kb on - strand at 157.524 kb on - strand at 157.524 kb on - strand at 157.524 kb on - strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.551 kb on + strand at 157.552 kb on - strand at 157.552 kb on - strand at 157.558 kb on + strand at 157.559 kb on - strand at 157.620 kb on - strand at 157.625 kb on + strand at 157.625 kb on + strand at 157.864 kb on + strand, within Psyr_0145 at 157.864 kb on + strand, within Psyr_0145 at 157.865 kb on - strand, within Psyr_0145 at 157.865 kb on - strand, within Psyr_0145 at 157.865 kb on - strand, within Psyr_0145 at 157.894 kb on + strand, within Psyr_0145 at 157.895 kb on - strand, within Psyr_0145 at 157.895 kb on - strand, within Psyr_0145 at 158.056 kb on + strand, within Psyr_0145 at 158.056 kb on + strand, within Psyr_0145 at 158.056 kb on + strand, within Psyr_0145 at 158.056 kb on + strand, within Psyr_0145 at 158.057 kb on - strand, within Psyr_0145 at 158.214 kb on - strand at 158.320 kb on - strand at 158.320 kb on - strand at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.699 kb on - strand, within Psyr_0146 at 159.127 kb on + strand, within Psyr_0146 at 159.127 kb on + strand, within Psyr_0146 at 159.128 kb on - strand, within Psyr_0146 at 159.128 kb on - strand, within Psyr_0146 at 159.167 kb on + strand, within Psyr_0146 at 159.167 kb on + strand, within Psyr_0146 at 159.341 kb on - strand, within Psyr_0146 at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.686 kb on + strand at 159.686 kb on + strand at 159.686 kb on + strand at 159.686 kb on + strand at 159.908 kb on + strand at 159.908 kb on + strand at 160.020 kb on - strand, within Psyr_0147 at 160.020 kb on - strand, within Psyr_0147 at 160.333 kb on + strand, within Psyr_0147 at 160.333 kb on + strand, within Psyr_0147 at 160.334 kb on - strand, within Psyr_0147 at 160.334 kb on - strand, within Psyr_0147 at 160.415 kb on + strand, within Psyr_0147 at 160.416 kb on - strand, within Psyr_0147 at 160.416 kb on - strand, within Psyr_0147 at 160.416 kb on - strand, within Psyr_0147 at 160.556 kb on - strand, within Psyr_0147 at 160.556 kb on - strand, within Psyr_0147
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 157,338 + Psyr_0144 0.63 +2.2 157,338 + Psyr_0144 0.63 +3.8 157,339 - Psyr_0144 0.63 -0.6 157,339 - Psyr_0144 0.63 +2.8 157,339 - Psyr_0144 0.63 -0.6 157,339 - Psyr_0144 0.63 +0.7 157,352 + Psyr_0144 0.66 -1.3 157,352 + Psyr_0144 0.66 +3.2 157,352 + Psyr_0144 0.66 -1.6 157,352 + Psyr_0144 0.66 +0.2 157,353 - Psyr_0144 0.66 -1.6 157,353 - Psyr_0144 0.66 +0.2 157,353 - Psyr_0144 0.66 -1.8 157,353 - Psyr_0144 0.66 +1.8 157,364 + Psyr_0144 0.68 -0.4 157,365 - Psyr_0144 0.69 -2.0 157,471 + -0.0 157,472 - -0.9 157,479 + +0.2 157,480 - +3.7 157,482 - +0.5 157,486 - -0.8 157,519 + +0.1 157,519 + +3.4 157,523 + +0.0 157,523 + -0.4 157,523 + -1.3 157,523 + +3.7 157,523 + +4.3 157,523 + -1.1 157,524 - -1.3 157,524 - -1.0 157,524 - +4.0 157,524 - +4.0 157,524 - -0.5 157,532 + -0.2 157,532 + +2.2 157,532 + +2.8 157,532 + +4.1 157,532 + -0.4 157,532 + +3.0 157,532 + -0.9 157,532 + -1.9 157,532 + -1.3 157,532 + -0.4 157,532 + -0.4 157,532 + +2.6 157,532 + -0.5 157,532 + +0.7 157,532 + +3.2 157,532 + -0.9 157,532 + -1.1 157,532 + +1.9 157,532 + -0.8 157,532 + +3.0 157,532 + -1.9 157,532 + -1.0 157,532 + +0.2 157,532 + +3.7 157,532 + +0.3 157,533 - +0.5 157,533 - -0.6 157,533 - -0.1 157,533 - -1.8 157,533 - +0.2 157,533 - -0.1 157,533 - +1.9 157,533 - +0.5 157,533 - +1.8 157,533 - +1.2 157,533 - +3.7 157,533 - +0.6 157,533 - -1.4 157,533 - -1.1 157,533 - +2.5 157,533 - +1.8 157,551 + -0.8 157,552 - +3.4 157,552 - -1.1 157,558 + -1.6 157,559 - +2.2 157,620 - +0.2 157,625 + +0.7 157,625 + -0.8 157,864 + Psyr_0145 0.44 +1.2 157,864 + Psyr_0145 0.44 -2.3 157,865 - Psyr_0145 0.44 -0.5 157,865 - Psyr_0145 0.44 +2.2 157,865 - Psyr_0145 0.44 -0.3 157,894 + Psyr_0145 0.49 +3.7 157,895 - Psyr_0145 0.49 +5.0 157,895 - Psyr_0145 0.49 -0.4 158,056 + Psyr_0145 0.73 +2.5 158,056 + Psyr_0145 0.73 +2.5 158,056 + Psyr_0145 0.73 +0.0 158,056 + Psyr_0145 0.73 +1.1 158,057 - Psyr_0145 0.73 -1.3 158,214 - +0.9 158,320 - -1.1 158,320 - -0.1 158,647 + Psyr_0146 0.26 +3.9 158,647 + Psyr_0146 0.26 -1.2 158,647 + Psyr_0146 0.26 +0.2 158,647 + Psyr_0146 0.26 -2.6 158,647 + Psyr_0146 0.26 +2.5 158,647 + Psyr_0146 0.26 +0.2 158,647 + Psyr_0146 0.26 +0.7 158,647 + Psyr_0146 0.26 -0.7 158,647 + Psyr_0146 0.26 -1.6 158,648 - Psyr_0146 0.26 -0.0 158,648 - Psyr_0146 0.26 -1.0 158,648 - Psyr_0146 0.26 -1.5 158,648 - Psyr_0146 0.26 -1.0 158,648 - Psyr_0146 0.26 -1.1 158,648 - Psyr_0146 0.26 +0.2 158,648 - Psyr_0146 0.26 -1.4 158,648 - Psyr_0146 0.26 -0.1 158,648 - Psyr_0146 0.26 -0.6 158,648 - Psyr_0146 0.26 -1.4 158,648 - Psyr_0146 0.26 -2.1 158,648 - Psyr_0146 0.26 +0.1 158,648 - Psyr_0146 0.26 +2.2 158,648 - Psyr_0146 0.26 -0.9 158,648 - Psyr_0146 0.26 +2.2 158,648 - Psyr_0146 0.26 +2.5 158,699 - Psyr_0146 0.30 -1.9 159,127 + Psyr_0146 0.63 +0.6 159,127 + Psyr_0146 0.63 +1.8 159,128 - Psyr_0146 0.63 -1.1 159,128 - Psyr_0146 0.63 -0.3 159,167 + Psyr_0146 0.66 +2.2 159,167 + Psyr_0146 0.66 -0.6 159,341 - Psyr_0146 0.79 -0.7 159,614 + +3.9 159,614 + +0.2 159,614 + +0.1 159,614 + -0.0 159,614 + +2.5 159,614 + +1.8 159,614 + +3.8 159,614 + -3.0 159,614 + -0.8 159,614 + +3.0 159,614 + -3.2 159,614 + +0.2 159,614 + -1.0 159,614 + +0.9 159,614 + +1.0 159,614 + -0.5 159,615 - -2.4 159,615 - +0.2 159,615 - +2.5 159,615 - +1.3 159,615 - +3.2 159,615 - -1.6 159,615 - -0.3 159,615 - +1.1 159,615 - +3.0 159,615 - +4.4 159,615 - -0.9 159,615 - +0.7 159,615 - -1.8 159,615 - -2.3 159,686 + +1.8 159,686 + +0.2 159,686 + -1.8 159,686 + -0.8 159,908 + -1.3 159,908 + +0.1 160,020 - Psyr_0147 0.14 +1.2 160,020 - Psyr_0147 0.14 +1.2 160,333 + Psyr_0147 0.47 +1.1 160,333 + Psyr_0147 0.47 +3.8 160,334 - Psyr_0147 0.47 -1.4 160,334 - Psyr_0147 0.47 +3.7 160,415 + Psyr_0147 0.56 -2.4 160,416 - Psyr_0147 0.56 +4.0 160,416 - Psyr_0147 0.56 -1.4 160,416 - Psyr_0147 0.56 -1.1 160,556 - Psyr_0147 0.71 -1.2 160,556 - Psyr_0147 0.71 +2.5
Or see this region's nucleotide sequence