Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0135 and Psyr_0136 are separated by 53 nucleotides Psyr_0136 and Psyr_0137 overlap by 1 nucleotides
Psyr_0135: Psyr_0135 - outer membrane transport energization protein TonB, at 144,401 to 145,225
_0135
Psyr_0136: Psyr_0136 - outer membrane transport energization protein ExbB, at 145,279 to 145,986
_0136
Psyr_0137: Psyr_0137 - outer membrane transport energization protein ExbD, at 145,986 to 146,387
_0137
Position (kb)
144
145
146 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 143.403 kb on - strand at 143.437 kb on + strand at 143.437 kb on + strand at 143.438 kb on - strand at 143.532 kb on + strand at 143.532 kb on + strand at 143.532 kb on + strand at 143.532 kb on + strand at 143.532 kb on + strand at 143.533 kb on - strand at 143.533 kb on - strand at 143.533 kb on - strand at 143.533 kb on - strand at 143.533 kb on - strand at 143.533 kb on - strand at 143.655 kb on - strand at 143.681 kb on + strand at 143.682 kb on - strand at 143.682 kb on - strand at 143.682 kb on - strand at 143.753 kb on + strand at 143.754 kb on - strand at 143.754 kb on - strand at 143.754 kb on - strand at 143.754 kb on - strand at 143.754 kb on - strand at 143.775 kb on + strand at 143.791 kb on + strand at 143.791 kb on + strand at 143.905 kb on + strand at 143.906 kb on - strand at 143.906 kb on - strand at 144.055 kb on + strand at 144.109 kb on - strand at 144.109 kb on - strand at 144.175 kb on + strand at 144.175 kb on + strand at 144.175 kb on + strand at 144.175 kb on + strand at 144.175 kb on + strand at 144.175 kb on + strand at 144.176 kb on - strand at 144.176 kb on - strand at 144.176 kb on - strand at 144.176 kb on - strand at 144.176 kb on - strand at 144.176 kb on - strand at 144.176 kb on - strand at 144.208 kb on - strand at 144.208 kb on - strand at 144.217 kb on + strand at 144.490 kb on + strand, within Psyr_0135 at 144.525 kb on + strand, within Psyr_0135 at 144.525 kb on + strand, within Psyr_0135 at 144.526 kb on - strand, within Psyr_0135 at 144.550 kb on - strand, within Psyr_0135 at 144.553 kb on + strand, within Psyr_0135 at 144.553 kb on + strand, within Psyr_0135 at 144.553 kb on + strand, within Psyr_0135 at 144.553 kb on + strand, within Psyr_0135 at 144.553 kb on + strand, within Psyr_0135 at 144.554 kb on - strand, within Psyr_0135 at 144.554 kb on - strand, within Psyr_0135 at 144.554 kb on - strand, within Psyr_0135 at 144.554 kb on - strand, within Psyr_0135 at 144.554 kb on - strand, within Psyr_0135 at 144.554 kb on - strand, within Psyr_0135 at 144.575 kb on + strand, within Psyr_0135 at 144.576 kb on - strand, within Psyr_0135 at 144.576 kb on - strand, within Psyr_0135 at 144.576 kb on - strand, within Psyr_0135 at 144.576 kb on - strand, within Psyr_0135 at 144.993 kb on - strand, within Psyr_0135 at 145.180 kb on - strand at 145.255 kb on - strand at 145.273 kb on + strand at 145.273 kb on + strand at 145.274 kb on - strand at 145.343 kb on + strand at 145.381 kb on + strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.382 kb on - strand, within Psyr_0136 at 145.472 kb on - strand, within Psyr_0136 at 145.491 kb on + strand, within Psyr_0136 at 145.607 kb on + strand, within Psyr_0136 at 145.607 kb on + strand, within Psyr_0136 at 145.608 kb on - strand, within Psyr_0136 at 145.834 kb on + strand, within Psyr_0136 at 145.834 kb on + strand, within Psyr_0136 at 145.835 kb on - strand, within Psyr_0136 at 145.835 kb on - strand, within Psyr_0136 at 145.835 kb on - strand, within Psyr_0136 at 145.984 kb on + strand at 145.984 kb on + strand at 145.985 kb on - strand at 146.199 kb on + strand, within Psyr_0137 at 146.199 kb on + strand, within Psyr_0137
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 143,403 - -1.3 143,437 + -1.5 143,437 + -1.2 143,438 - -0.4 143,532 + +0.2 143,532 + -1.1 143,532 + +0.3 143,532 + +3.7 143,532 + -0.5 143,533 - +1.2 143,533 - +1.2 143,533 - +2.8 143,533 - +1.8 143,533 - -0.4 143,533 - +1.1 143,655 - +0.9 143,681 + +0.2 143,682 - +3.2 143,682 - -0.4 143,682 - +4.5 143,753 + -0.9 143,754 - -1.1 143,754 - +3.2 143,754 - -1.3 143,754 - +2.8 143,754 - -1.5 143,775 + -2.6 143,791 + +0.3 143,791 + +0.3 143,905 + +3.2 143,906 - +3.2 143,906 - -1.2 144,055 + +0.6 144,109 - -1.4 144,109 - -0.7 144,175 + -0.6 144,175 + +0.2 144,175 + -2.3 144,175 + -0.1 144,175 + -2.5 144,175 + +1.8 144,176 - -1.0 144,176 - -1.0 144,176 - +1.4 144,176 - +0.5 144,176 - -3.0 144,176 - -2.6 144,176 - +3.0 144,208 - +0.6 144,208 - -0.1 144,217 + -0.6 144,490 + Psyr_0135 0.11 +0.0 144,525 + Psyr_0135 0.15 +1.8 144,525 + Psyr_0135 0.15 -0.4 144,526 - Psyr_0135 0.15 +1.8 144,550 - Psyr_0135 0.18 -0.9 144,553 + Psyr_0135 0.18 -1.4 144,553 + Psyr_0135 0.18 +2.5 144,553 + Psyr_0135 0.18 -1.6 144,553 + Psyr_0135 0.18 +0.6 144,553 + Psyr_0135 0.18 +0.2 144,554 - Psyr_0135 0.19 -3.7 144,554 - Psyr_0135 0.19 +3.7 144,554 - Psyr_0135 0.19 +1.2 144,554 - Psyr_0135 0.19 +0.2 144,554 - Psyr_0135 0.19 +1.8 144,554 - Psyr_0135 0.19 +5.3 144,575 + Psyr_0135 0.21 +2.5 144,576 - Psyr_0135 0.21 -2.3 144,576 - Psyr_0135 0.21 -3.7 144,576 - Psyr_0135 0.21 -0.9 144,576 - Psyr_0135 0.21 -2.3 144,993 - Psyr_0135 0.72 -1.3 145,180 - -0.4 145,255 - -0.5 145,273 + -0.0 145,273 + +3.0 145,274 - -0.8 145,343 + +2.5 145,381 + Psyr_0136 0.14 +2.5 145,382 - Psyr_0136 0.15 +2.8 145,382 - Psyr_0136 0.15 -1.5 145,382 - Psyr_0136 0.15 -4.4 145,382 - Psyr_0136 0.15 -1.2 145,382 - Psyr_0136 0.15 +3.7 145,382 - Psyr_0136 0.15 -1.4 145,472 - Psyr_0136 0.27 +0.2 145,491 + Psyr_0136 0.30 -2.1 145,607 + Psyr_0136 0.46 +2.8 145,607 + Psyr_0136 0.46 -1.2 145,608 - Psyr_0136 0.46 -3.7 145,834 + Psyr_0136 0.78 +3.2 145,834 + Psyr_0136 0.78 -1.0 145,835 - Psyr_0136 0.79 +4.3 145,835 - Psyr_0136 0.79 +0.6 145,835 - Psyr_0136 0.79 -0.0 145,984 + -1.0 145,984 + +1.6 145,985 - +1.3 146,199 + Psyr_0137 0.53 +0.6 146,199 + Psyr_0137 0.53 -0.4
Or see this region's nucleotide sequence