Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0108 and Psyr_0109 are separated by 414 nucleotides Psyr_0109 and Psyr_0110 are separated by 363 nucleotides
Psyr_0108: Psyr_0108 - Major facilitator superfamily, at 118,946 to 120,304
_0108
Psyr_0109: Psyr_0109 - hypothetical protein, at 120,719 to 121,144
_0109
Psyr_0110: Psyr_0110 - hypothetical protein, at 121,508 to 122,362
_0110
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 119.958 kb on - strand, within Psyr_0108 at 120.110 kb on + strand, within Psyr_0108 at 120.150 kb on - strand, within Psyr_0108 at 120.150 kb on - strand, within Psyr_0108 at 120.260 kb on + strand at 120.349 kb on + strand at 120.349 kb on + strand at 120.349 kb on + strand at 120.349 kb on + strand at 120.349 kb on + strand at 120.350 kb on - strand at 120.350 kb on - strand at 120.350 kb on - strand at 120.350 kb on - strand at 120.519 kb on + strand at 120.519 kb on + strand at 120.520 kb on - strand at 120.520 kb on - strand at 120.520 kb on - strand at 120.622 kb on + strand at 120.649 kb on - strand at 120.664 kb on + strand at 120.665 kb on - strand at 120.680 kb on + strand at 120.680 kb on + strand at 120.680 kb on + strand at 120.681 kb on - strand at 120.681 kb on - strand at 120.688 kb on + strand at 120.688 kb on + strand at 120.688 kb on + strand at 120.689 kb on - strand at 120.693 kb on + strand at 120.694 kb on - strand at 120.694 kb on - strand at 120.694 kb on - strand at 120.694 kb on - strand at 120.741 kb on - strand at 120.741 kb on - strand at 120.741 kb on - strand at 120.759 kb on + strand at 120.759 kb on + strand at 120.760 kb on - strand at 120.760 kb on - strand at 120.769 kb on + strand, within Psyr_0109 at 120.769 kb on + strand, within Psyr_0109 at 120.829 kb on + strand, within Psyr_0109 at 120.908 kb on - strand, within Psyr_0109 at 120.909 kb on + strand, within Psyr_0109 at 120.910 kb on - strand, within Psyr_0109 at 120.910 kb on - strand, within Psyr_0109 at 120.917 kb on + strand, within Psyr_0109 at 120.917 kb on + strand, within Psyr_0109 at 120.918 kb on - strand, within Psyr_0109 at 120.918 kb on - strand, within Psyr_0109 at 120.918 kb on - strand, within Psyr_0109 at 120.918 kb on - strand, within Psyr_0109 at 120.927 kb on - strand, within Psyr_0109 at 120.939 kb on + strand, within Psyr_0109 at 120.950 kb on + strand, within Psyr_0109 at 121.001 kb on - strand, within Psyr_0109 at 121.001 kb on - strand, within Psyr_0109 at 121.019 kb on + strand, within Psyr_0109 at 121.019 kb on + strand, within Psyr_0109 at 121.019 kb on + strand, within Psyr_0109 at 121.019 kb on + strand, within Psyr_0109 at 121.019 kb on + strand, within Psyr_0109 at 121.019 kb on + strand, within Psyr_0109 at 121.020 kb on - strand, within Psyr_0109 at 121.020 kb on - strand, within Psyr_0109 at 121.020 kb on - strand, within Psyr_0109 at 121.021 kb on + strand, within Psyr_0109 at 121.022 kb on - strand, within Psyr_0109 at 121.091 kb on + strand, within Psyr_0109 at 121.126 kb on + strand at 121.126 kb on + strand at 121.127 kb on - strand at 121.139 kb on - strand at 121.139 kb on - strand at 121.142 kb on + strand at 121.239 kb on - strand at 121.239 kb on - strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.252 kb on - strand at 121.252 kb on - strand at 121.255 kb on + strand at 121.255 kb on + strand at 121.255 kb on + strand at 121.255 kb on + strand at 121.255 kb on + strand at 121.256 kb on - strand at 121.256 kb on - strand at 121.256 kb on - strand at 121.338 kb on + strand at 121.338 kb on + strand at 121.339 kb on - strand at 121.364 kb on + strand at 121.364 kb on + strand at 121.365 kb on - strand at 121.412 kb on + strand at 121.412 kb on + strand at 121.459 kb on - strand at 121.505 kb on + strand at 121.505 kb on + strand at 121.505 kb on + strand at 121.505 kb on + strand at 121.505 kb on + strand at 121.505 kb on + strand at 121.505 kb on + strand at 121.505 kb on + strand at 121.505 kb on + strand at 121.505 kb on + strand at 121.505 kb on + strand at 121.506 kb on - strand at 121.506 kb on - strand at 121.506 kb on - strand at 121.506 kb on - strand at 121.522 kb on - strand at 121.550 kb on + strand at 121.551 kb on - strand at 121.551 kb on - strand at 121.551 kb on - strand at 121.551 kb on - strand at 121.565 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.571 kb on + strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.572 kb on - strand at 121.654 kb on + strand, within Psyr_0110 at 121.655 kb on - strand, within Psyr_0110 at 121.689 kb on + strand, within Psyr_0110 at 121.792 kb on - strand, within Psyr_0110 at 121.836 kb on + strand, within Psyr_0110 at 121.836 kb on + strand, within Psyr_0110 at 121.836 kb on + strand, within Psyr_0110 at 121.836 kb on + strand, within Psyr_0110 at 121.836 kb on + strand, within Psyr_0110 at 121.837 kb on - strand, within Psyr_0110 at 121.837 kb on - strand, within Psyr_0110 at 121.865 kb on + strand, within Psyr_0110 at 121.866 kb on - strand, within Psyr_0110 at 121.866 kb on - strand, within Psyr_0110 at 121.866 kb on - strand, within Psyr_0110 at 121.866 kb on - strand, within Psyr_0110 at 121.899 kb on + strand, within Psyr_0110 at 121.915 kb on + strand, within Psyr_0110 at 121.916 kb on - strand, within Psyr_0110 at 121.916 kb on - strand, within Psyr_0110 at 121.931 kb on - strand, within Psyr_0110 at 121.931 kb on - strand, within Psyr_0110 at 121.956 kb on - strand, within Psyr_0110 at 121.967 kb on + strand, within Psyr_0110 at 121.967 kb on + strand, within Psyr_0110 at 121.967 kb on + strand, within Psyr_0110 at 121.967 kb on + strand, within Psyr_0110 at 121.967 kb on + strand, within Psyr_0110 at 121.967 kb on + strand, within Psyr_0110 at 121.967 kb on + strand, within Psyr_0110 at 121.967 kb on + strand, within Psyr_0110 at 121.967 kb on + strand, within Psyr_0110 at 121.967 kb on + strand, within Psyr_0110 at 121.967 kb on + strand, within Psyr_0110 at 121.967 kb on + strand, within Psyr_0110 at 121.968 kb on - strand, within Psyr_0110 at 121.968 kb on - strand, within Psyr_0110 at 121.968 kb on - strand, within Psyr_0110 at 121.968 kb on - strand, within Psyr_0110 at 121.968 kb on - strand, within Psyr_0110 at 121.968 kb on - strand, within Psyr_0110 at 121.968 kb on - strand, within Psyr_0110 at 121.968 kb on - strand, within Psyr_0110 at 121.968 kb on - strand, within Psyr_0110 at 121.968 kb on - strand, within Psyr_0110 at 121.968 kb on - strand, within Psyr_0110 at 121.968 kb on - strand, within Psyr_0110 at 122.012 kb on + strand, within Psyr_0110 at 122.013 kb on - strand, within Psyr_0110 at 122.141 kb on + strand, within Psyr_0110 at 122.141 kb on + strand, within Psyr_0110 at 122.141 kb on + strand, within Psyr_0110 at 122.141 kb on + strand, within Psyr_0110 at 122.141 kb on + strand, within Psyr_0110 at 122.141 kb on + strand, within Psyr_0110 at 122.141 kb on + strand, within Psyr_0110 at 122.142 kb on - strand, within Psyr_0110 at 122.142 kb on - strand, within Psyr_0110 at 122.142 kb on - strand, within Psyr_0110
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 119,958 - Psyr_0108 0.74 +2.8 120,110 + Psyr_0108 0.86 -0.9 120,150 - Psyr_0108 0.89 -0.5 120,150 - Psyr_0108 0.89 +3.2 120,260 + -0.3 120,349 + +0.2 120,349 + -0.6 120,349 + +3.0 120,349 + +3.8 120,349 + +2.5 120,350 - +0.0 120,350 - +2.5 120,350 - +0.2 120,350 - -0.4 120,519 + -1.9 120,519 + +3.0 120,520 - -0.4 120,520 - +3.5 120,520 - +0.2 120,622 + +4.1 120,649 - -0.2 120,664 + -3.4 120,665 - -2.0 120,680 + -0.7 120,680 + -0.9 120,680 + -0.3 120,681 - +1.4 120,681 - +2.5 120,688 + -1.2 120,688 + +0.5 120,688 + +0.4 120,689 - -1.0 120,693 + -2.2 120,694 - +0.8 120,694 - -0.3 120,694 - +1.2 120,694 - +1.0 120,741 - -1.1 120,741 - +3.9 120,741 - -0.9 120,759 + -5.2 120,759 + -1.9 120,760 - -1.7 120,760 - +0.2 120,769 + Psyr_0109 0.12 -2.6 120,769 + Psyr_0109 0.12 -1.9 120,829 + Psyr_0109 0.26 +4.6 120,908 - Psyr_0109 0.44 +2.5 120,909 + Psyr_0109 0.45 -1.5 120,910 - Psyr_0109 0.45 +0.5 120,910 - Psyr_0109 0.45 -1.1 120,917 + Psyr_0109 0.46 -0.8 120,917 + Psyr_0109 0.46 -0.0 120,918 - Psyr_0109 0.47 +2.2 120,918 - Psyr_0109 0.47 +4.0 120,918 - Psyr_0109 0.47 +2.2 120,918 - Psyr_0109 0.47 -0.7 120,927 - Psyr_0109 0.49 -2.4 120,939 + Psyr_0109 0.52 +3.2 120,950 + Psyr_0109 0.54 -2.1 121,001 - Psyr_0109 0.66 -1.1 121,001 - Psyr_0109 0.66 +3.7 121,019 + Psyr_0109 0.70 -0.5 121,019 + Psyr_0109 0.70 +0.2 121,019 + Psyr_0109 0.70 +3.2 121,019 + Psyr_0109 0.70 +3.7 121,019 + Psyr_0109 0.70 -0.0 121,019 + Psyr_0109 0.70 +1.8 121,020 - Psyr_0109 0.71 -1.0 121,020 - Psyr_0109 0.71 +3.2 121,020 - Psyr_0109 0.71 -0.2 121,021 + Psyr_0109 0.71 -1.4 121,022 - Psyr_0109 0.71 +3.7 121,091 + Psyr_0109 0.87 -1.6 121,126 + +2.2 121,126 + -0.2 121,127 - -4.1 121,139 - -0.3 121,139 - -2.9 121,142 + +1.2 121,239 - -2.0 121,239 - -1.8 121,251 + -1.0 121,251 + -1.7 121,251 + -0.9 121,251 + +0.7 121,251 + -1.1 121,251 + +3.2 121,251 + -1.5 121,251 + -5.1 121,251 + +1.8 121,252 - -1.8 121,252 - -0.5 121,255 + -2.0 121,255 + -0.1 121,255 + +3.7 121,255 + +0.8 121,255 + +0.1 121,256 - -0.7 121,256 - +2.2 121,256 - -2.4 121,338 + -0.8 121,338 + -0.1 121,339 - -2.1 121,364 + +1.2 121,364 + -0.6 121,365 - +3.9 121,412 + +4.0 121,412 + -1.2 121,459 - -0.2 121,505 + +0.1 121,505 + +0.2 121,505 + +2.2 121,505 + +0.4 121,505 + -0.5 121,505 + +2.5 121,505 + -1.0 121,505 + +1.1 121,505 + -2.1 121,505 + -1.0 121,505 + -1.9 121,506 - +0.2 121,506 - -3.6 121,506 - +0.6 121,506 - -2.8 121,522 - -0.1 121,550 + -1.4 121,551 - +3.5 121,551 - -2.5 121,551 - -1.2 121,551 - +1.2 121,565 + +0.1 121,571 + +0.4 121,571 + +2.8 121,571 + -4.0 121,571 + -1.9 121,571 + -0.5 121,571 + -2.0 121,571 + +3.2 121,571 + +1.6 121,571 + +2.2 121,571 + +0.5 121,571 + -0.6 121,571 + -0.2 121,571 + -0.2 121,571 + -0.2 121,571 + -0.6 121,572 - -0.7 121,572 - -1.0 121,572 - +0.1 121,572 - +1.8 121,572 - +1.9 121,572 - -2.8 121,572 - +1.6 121,572 - -3.6 121,572 - -0.9 121,572 - +2.2 121,572 - +2.5 121,572 - +0.2 121,572 - -2.3 121,572 - -1.6 121,654 + Psyr_0110 0.17 +2.1 121,655 - Psyr_0110 0.17 -2.5 121,689 + Psyr_0110 0.21 -1.1 121,792 - Psyr_0110 0.33 -1.8 121,836 + Psyr_0110 0.38 -1.3 121,836 + Psyr_0110 0.38 +0.3 121,836 + Psyr_0110 0.38 +0.2 121,836 + Psyr_0110 0.38 -0.9 121,836 + Psyr_0110 0.38 +0.2 121,837 - Psyr_0110 0.38 +1.2 121,837 - Psyr_0110 0.38 +1.8 121,865 + Psyr_0110 0.42 +0.3 121,866 - Psyr_0110 0.42 -0.1 121,866 - Psyr_0110 0.42 +0.2 121,866 - Psyr_0110 0.42 -0.5 121,866 - Psyr_0110 0.42 -1.1 121,899 + Psyr_0110 0.46 +1.1 121,915 + Psyr_0110 0.48 -0.5 121,916 - Psyr_0110 0.48 -1.2 121,916 - Psyr_0110 0.48 +0.5 121,931 - Psyr_0110 0.49 +1.2 121,931 - Psyr_0110 0.49 -2.1 121,956 - Psyr_0110 0.52 -0.9 121,967 + Psyr_0110 0.54 -1.3 121,967 + Psyr_0110 0.54 -0.2 121,967 + Psyr_0110 0.54 -0.6 121,967 + Psyr_0110 0.54 +0.1 121,967 + Psyr_0110 0.54 -0.0 121,967 + Psyr_0110 0.54 -1.0 121,967 + Psyr_0110 0.54 -2.1 121,967 + Psyr_0110 0.54 +3.8 121,967 + Psyr_0110 0.54 -0.0 121,967 + Psyr_0110 0.54 +2.0 121,967 + Psyr_0110 0.54 -1.4 121,967 + Psyr_0110 0.54 -2.5 121,968 - Psyr_0110 0.54 -2.0 121,968 - Psyr_0110 0.54 -0.0 121,968 - Psyr_0110 0.54 +4.3 121,968 - Psyr_0110 0.54 -1.6 121,968 - Psyr_0110 0.54 -0.1 121,968 - Psyr_0110 0.54 -0.8 121,968 - Psyr_0110 0.54 -0.5 121,968 - Psyr_0110 0.54 -2.0 121,968 - Psyr_0110 0.54 +3.5 121,968 - Psyr_0110 0.54 -1.7 121,968 - Psyr_0110 0.54 -0.8 121,968 - Psyr_0110 0.54 -1.1 122,012 + Psyr_0110 0.59 -1.7 122,013 - Psyr_0110 0.59 -0.9 122,141 + Psyr_0110 0.74 +0.2 122,141 + Psyr_0110 0.74 -0.9 122,141 + Psyr_0110 0.74 -0.6 122,141 + Psyr_0110 0.74 -0.5 122,141 + Psyr_0110 0.74 -1.3 122,141 + Psyr_0110 0.74 +4.0 122,141 + Psyr_0110 0.74 -2.5 122,142 - Psyr_0110 0.74 +0.2 122,142 - Psyr_0110 0.74 -1.8 122,142 - Psyr_0110 0.74 -0.6
Or see this region's nucleotide sequence