Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0106 and Psyr_0107 are separated by 201 nucleotides Psyr_0107 and Psyr_0108 are separated by 210 nucleotides Psyr_0108 and Psyr_0109 are separated by 414 nucleotides
Psyr_0106: Psyr_0106 - Transposase IS3/IS911, at 117,869 to 118,168
_0106
Psyr_0107: Psyr_0107 - hypothetical protein, at 118,370 to 118,735
_0107
Psyr_0108: Psyr_0108 - Major facilitator superfamily, at 118,946 to 120,304
_0108
Psyr_0109: Psyr_0109 - hypothetical protein, at 120,719 to 121,144
_0109
Position (kb)
118
119
120
121 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 118.207 kb on + strand at 118.235 kb on - strand at 118.249 kb on + strand at 118.282 kb on + strand at 118.283 kb on - strand at 118.283 kb on - strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.292 kb on + strand at 118.293 kb on - strand at 118.293 kb on - strand at 118.293 kb on - strand at 118.293 kb on - strand at 118.293 kb on - strand at 118.293 kb on - strand at 118.296 kb on + strand at 118.296 kb on + strand at 118.296 kb on + strand at 118.296 kb on + strand at 118.296 kb on + strand at 118.297 kb on - strand at 118.297 kb on - strand at 118.297 kb on - strand at 118.297 kb on - strand at 118.297 kb on - strand at 118.297 kb on - strand at 118.297 kb on - strand at 118.319 kb on + strand at 118.319 kb on + strand at 118.319 kb on + strand at 118.319 kb on + strand at 118.320 kb on - strand at 118.320 kb on - strand at 118.320 kb on - strand at 118.519 kb on + strand, within Psyr_0107 at 118.520 kb on - strand, within Psyr_0107 at 118.605 kb on + strand, within Psyr_0107 at 118.606 kb on - strand, within Psyr_0107 at 118.609 kb on + strand, within Psyr_0107 at 118.609 kb on + strand, within Psyr_0107 at 118.610 kb on - strand, within Psyr_0107 at 118.666 kb on + strand, within Psyr_0107 at 118.666 kb on + strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.667 kb on - strand, within Psyr_0107 at 118.678 kb on + strand, within Psyr_0107 at 118.678 kb on + strand, within Psyr_0107 at 118.678 kb on + strand, within Psyr_0107 at 118.679 kb on - strand, within Psyr_0107 at 118.679 kb on - strand, within Psyr_0107 at 118.679 kb on - strand, within Psyr_0107 at 118.679 kb on - strand, within Psyr_0107 at 118.679 kb on - strand, within Psyr_0107 at 118.679 kb on - strand, within Psyr_0107 at 118.776 kb on + strand at 118.823 kb on + strand at 118.824 kb on - strand at 118.824 kb on - strand at 118.981 kb on + strand at 119.064 kb on + strand at 119.065 kb on - strand at 119.065 kb on - strand at 119.075 kb on + strand at 119.141 kb on + strand, within Psyr_0108 at 119.141 kb on + strand, within Psyr_0108 at 119.141 kb on + strand, within Psyr_0108 at 119.159 kb on - strand, within Psyr_0108 at 119.192 kb on - strand, within Psyr_0108 at 119.285 kb on + strand, within Psyr_0108 at 119.285 kb on + strand, within Psyr_0108 at 119.286 kb on - strand, within Psyr_0108 at 119.286 kb on - strand, within Psyr_0108 at 119.286 kb on - strand, within Psyr_0108 at 119.286 kb on - strand, within Psyr_0108 at 119.286 kb on - strand, within Psyr_0108 at 119.306 kb on + strand, within Psyr_0108 at 119.306 kb on + strand, within Psyr_0108 at 119.306 kb on + strand, within Psyr_0108 at 119.306 kb on + strand, within Psyr_0108 at 119.306 kb on + strand, within Psyr_0108 at 119.306 kb on + strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.307 kb on - strand, within Psyr_0108 at 119.343 kb on - strand, within Psyr_0108 at 119.958 kb on - strand, within Psyr_0108 at 120.110 kb on + strand, within Psyr_0108 at 120.150 kb on - strand, within Psyr_0108 at 120.150 kb on - strand, within Psyr_0108 at 120.260 kb on + strand at 120.349 kb on + strand at 120.349 kb on + strand at 120.349 kb on + strand at 120.349 kb on + strand at 120.349 kb on + strand at 120.350 kb on - strand at 120.350 kb on - strand at 120.350 kb on - strand at 120.350 kb on - strand at 120.519 kb on + strand at 120.519 kb on + strand at 120.520 kb on - strand at 120.520 kb on - strand at 120.520 kb on - strand at 120.622 kb on + strand at 120.649 kb on - strand at 120.664 kb on + strand at 120.665 kb on - strand at 120.680 kb on + strand at 120.680 kb on + strand at 120.680 kb on + strand at 120.681 kb on - strand at 120.681 kb on - strand at 120.688 kb on + strand at 120.688 kb on + strand at 120.688 kb on + strand at 120.689 kb on - strand at 120.693 kb on + strand at 120.694 kb on - strand at 120.694 kb on - strand at 120.694 kb on - strand at 120.694 kb on - strand at 120.741 kb on - strand at 120.741 kb on - strand at 120.741 kb on - strand at 120.759 kb on + strand at 120.759 kb on + strand at 120.760 kb on - strand at 120.760 kb on - strand at 120.769 kb on + strand, within Psyr_0109 at 120.769 kb on + strand, within Psyr_0109 at 120.829 kb on + strand, within Psyr_0109 at 120.908 kb on - strand, within Psyr_0109 at 120.909 kb on + strand, within Psyr_0109 at 120.910 kb on - strand, within Psyr_0109 at 120.910 kb on - strand, within Psyr_0109 at 120.917 kb on + strand, within Psyr_0109 at 120.917 kb on + strand, within Psyr_0109 at 120.918 kb on - strand, within Psyr_0109 at 120.918 kb on - strand, within Psyr_0109 at 120.918 kb on - strand, within Psyr_0109 at 120.918 kb on - strand, within Psyr_0109 at 120.927 kb on - strand, within Psyr_0109 at 120.939 kb on + strand, within Psyr_0109 at 120.950 kb on + strand, within Psyr_0109 at 121.001 kb on - strand, within Psyr_0109 at 121.001 kb on - strand, within Psyr_0109 at 121.019 kb on + strand, within Psyr_0109 at 121.019 kb on + strand, within Psyr_0109 at 121.019 kb on + strand, within Psyr_0109 at 121.019 kb on + strand, within Psyr_0109 at 121.019 kb on + strand, within Psyr_0109 at 121.019 kb on + strand, within Psyr_0109 at 121.020 kb on - strand, within Psyr_0109 at 121.020 kb on - strand, within Psyr_0109 at 121.020 kb on - strand, within Psyr_0109 at 121.021 kb on + strand, within Psyr_0109 at 121.022 kb on - strand, within Psyr_0109 at 121.091 kb on + strand, within Psyr_0109 at 121.126 kb on + strand at 121.126 kb on + strand at 121.127 kb on - strand at 121.139 kb on - strand at 121.139 kb on - strand at 121.142 kb on + strand at 121.239 kb on - strand at 121.239 kb on - strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.251 kb on + strand at 121.252 kb on - strand at 121.252 kb on - strand at 121.255 kb on + strand at 121.255 kb on + strand at 121.255 kb on + strand at 121.255 kb on + strand at 121.255 kb on + strand at 121.256 kb on - strand at 121.256 kb on - strand at 121.256 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 118,207 + +4.4 118,235 - +1.0 118,249 + -0.8 118,282 + -2.2 118,283 - +0.0 118,283 - +2.8 118,292 + -1.7 118,292 + +3.7 118,292 + -0.8 118,292 + -0.2 118,292 + +0.9 118,292 + +1.4 118,292 + -4.4 118,292 + +1.8 118,293 - -2.8 118,293 - +3.9 118,293 - +2.8 118,293 - +1.3 118,293 - -1.5 118,293 - -2.0 118,296 + +2.8 118,296 + +0.8 118,296 + -1.0 118,296 + -0.6 118,296 + +2.5 118,297 - -0.8 118,297 - -0.8 118,297 - -0.4 118,297 - -1.1 118,297 - +1.2 118,297 - +1.8 118,297 - +3.9 118,319 + +3.5 118,319 + -2.1 118,319 + -0.8 118,319 + -0.7 118,320 - +0.5 118,320 - -2.1 118,320 - +0.2 118,519 + Psyr_0107 0.41 -1.8 118,520 - Psyr_0107 0.41 +3.9 118,605 + Psyr_0107 0.64 +3.4 118,606 - Psyr_0107 0.64 -0.2 118,609 + Psyr_0107 0.65 +3.5 118,609 + Psyr_0107 0.65 -0.0 118,610 - Psyr_0107 0.66 +1.8 118,666 + Psyr_0107 0.81 -1.6 118,666 + Psyr_0107 0.81 -0.3 118,667 - Psyr_0107 0.81 -0.3 118,667 - Psyr_0107 0.81 +4.7 118,667 - Psyr_0107 0.81 -0.5 118,667 - Psyr_0107 0.81 -1.8 118,667 - Psyr_0107 0.81 +0.8 118,667 - Psyr_0107 0.81 -2.2 118,667 - Psyr_0107 0.81 +1.4 118,678 + Psyr_0107 0.84 -1.3 118,678 + Psyr_0107 0.84 +1.5 118,678 + Psyr_0107 0.84 +0.2 118,679 - Psyr_0107 0.84 +3.2 118,679 - Psyr_0107 0.84 +0.5 118,679 - Psyr_0107 0.84 +0.1 118,679 - Psyr_0107 0.84 -1.6 118,679 - Psyr_0107 0.84 +2.2 118,679 - Psyr_0107 0.84 +3.8 118,776 + +4.5 118,823 + +0.2 118,824 - -0.2 118,824 - -0.1 118,981 + -3.2 119,064 + +2.5 119,065 - +2.5 119,065 - -3.2 119,075 + -0.4 119,141 + Psyr_0108 0.14 +2.5 119,141 + Psyr_0108 0.14 +2.5 119,141 + Psyr_0108 0.14 -2.0 119,159 - Psyr_0108 0.16 -0.5 119,192 - Psyr_0108 0.18 +0.3 119,285 + Psyr_0108 0.25 +2.8 119,285 + Psyr_0108 0.25 -1.4 119,286 - Psyr_0108 0.25 -0.5 119,286 - Psyr_0108 0.25 +3.8 119,286 - Psyr_0108 0.25 +0.5 119,286 - Psyr_0108 0.25 -0.5 119,286 - Psyr_0108 0.25 +3.0 119,306 + Psyr_0108 0.26 +4.7 119,306 + Psyr_0108 0.26 +0.0 119,306 + Psyr_0108 0.26 -1.4 119,306 + Psyr_0108 0.26 -2.1 119,306 + Psyr_0108 0.26 -0.4 119,306 + Psyr_0108 0.26 +0.4 119,307 - Psyr_0108 0.27 -1.2 119,307 - Psyr_0108 0.27 +2.5 119,307 - Psyr_0108 0.27 -1.8 119,307 - Psyr_0108 0.27 +4.0 119,307 - Psyr_0108 0.27 -1.3 119,307 - Psyr_0108 0.27 +2.2 119,307 - Psyr_0108 0.27 +1.7 119,343 - Psyr_0108 0.29 +0.7 119,958 - Psyr_0108 0.74 +2.8 120,110 + Psyr_0108 0.86 -0.9 120,150 - Psyr_0108 0.89 -0.5 120,150 - Psyr_0108 0.89 +3.2 120,260 + -0.3 120,349 + +0.2 120,349 + -0.6 120,349 + +3.0 120,349 + +3.8 120,349 + +2.5 120,350 - +0.0 120,350 - +2.5 120,350 - +0.2 120,350 - -0.4 120,519 + -1.9 120,519 + +3.0 120,520 - -0.4 120,520 - +3.5 120,520 - +0.2 120,622 + +4.1 120,649 - -0.2 120,664 + -3.4 120,665 - -2.0 120,680 + -0.7 120,680 + -0.9 120,680 + -0.3 120,681 - +1.4 120,681 - +2.5 120,688 + -1.2 120,688 + +0.5 120,688 + +0.4 120,689 - -1.0 120,693 + -2.2 120,694 - +0.8 120,694 - -0.3 120,694 - +1.2 120,694 - +1.0 120,741 - -1.1 120,741 - +3.9 120,741 - -0.9 120,759 + -5.2 120,759 + -1.9 120,760 - -1.7 120,760 - +0.2 120,769 + Psyr_0109 0.12 -2.6 120,769 + Psyr_0109 0.12 -1.9 120,829 + Psyr_0109 0.26 +4.6 120,908 - Psyr_0109 0.44 +2.5 120,909 + Psyr_0109 0.45 -1.5 120,910 - Psyr_0109 0.45 +0.5 120,910 - Psyr_0109 0.45 -1.1 120,917 + Psyr_0109 0.46 -0.8 120,917 + Psyr_0109 0.46 -0.0 120,918 - Psyr_0109 0.47 +2.2 120,918 - Psyr_0109 0.47 +4.0 120,918 - Psyr_0109 0.47 +2.2 120,918 - Psyr_0109 0.47 -0.7 120,927 - Psyr_0109 0.49 -2.4 120,939 + Psyr_0109 0.52 +3.2 120,950 + Psyr_0109 0.54 -2.1 121,001 - Psyr_0109 0.66 -1.1 121,001 - Psyr_0109 0.66 +3.7 121,019 + Psyr_0109 0.70 -0.5 121,019 + Psyr_0109 0.70 +0.2 121,019 + Psyr_0109 0.70 +3.2 121,019 + Psyr_0109 0.70 +3.7 121,019 + Psyr_0109 0.70 -0.0 121,019 + Psyr_0109 0.70 +1.8 121,020 - Psyr_0109 0.71 -1.0 121,020 - Psyr_0109 0.71 +3.2 121,020 - Psyr_0109 0.71 -0.2 121,021 + Psyr_0109 0.71 -1.4 121,022 - Psyr_0109 0.71 +3.7 121,091 + Psyr_0109 0.87 -1.6 121,126 + +2.2 121,126 + -0.2 121,127 - -4.1 121,139 - -0.3 121,139 - -2.9 121,142 + +1.2 121,239 - -2.0 121,239 - -1.8 121,251 + -1.0 121,251 + -1.7 121,251 + -0.9 121,251 + +0.7 121,251 + -1.1 121,251 + +3.2 121,251 + -1.5 121,251 + -5.1 121,251 + +1.8 121,252 - -1.8 121,252 - -0.5 121,255 + -2.0 121,255 + -0.1 121,255 + +3.7 121,255 + +0.8 121,255 + +0.1 121,256 - -0.7 121,256 - +2.2 121,256 - -2.4
Or see this region's nucleotide sequence