Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0078 and Psyr_0079 are separated by 27 nucleotides Psyr_0079 and Psyr_0080 overlap by 8 nucleotides
Psyr_0078: Psyr_0078 - Beta-lactamase-like protein, at 84,310 to 85,194
_0078
Psyr_0079: Psyr_0079 - conserved hypothetical protein, at 85,222 to 86,475
_0079
Psyr_0080: Psyr_0080 - Protein of unknown function DUF81, at 86,468 to 87,280
_0080
Position (kb)
85
86
87 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 84.316 kb on - strand at 84.357 kb on + strand at 84.357 kb on + strand at 84.357 kb on + strand at 84.357 kb on + strand at 84.358 kb on - strand at 84.358 kb on - strand at 84.358 kb on - strand at 84.358 kb on - strand at 84.358 kb on - strand at 84.358 kb on - strand at 84.373 kb on + strand at 84.374 kb on - strand at 84.374 kb on - strand at 84.431 kb on - strand, within Psyr_0078 at 84.431 kb on - strand, within Psyr_0078 at 84.431 kb on - strand, within Psyr_0078 at 84.949 kb on + strand, within Psyr_0078 at 84.949 kb on + strand, within Psyr_0078 at 84.949 kb on + strand, within Psyr_0078 at 84.950 kb on - strand at 84.976 kb on + strand, within Psyr_0078 at 84.976 kb on + strand, within Psyr_0078 at 84.976 kb on + strand, within Psyr_0078 at 84.976 kb on + strand, within Psyr_0078 at 84.976 kb on + strand, within Psyr_0078 at 84.976 kb on + strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 85.355 kb on - strand, within Psyr_0079 at 85.355 kb on - strand, within Psyr_0079 at 85.355 kb on - strand, within Psyr_0079 at 85.358 kb on - strand, within Psyr_0079 at 85.358 kb on - strand, within Psyr_0079 at 85.358 kb on - strand, within Psyr_0079 at 85.358 kb on - strand, within Psyr_0079 at 85.621 kb on + strand, within Psyr_0079 at 85.643 kb on - strand, within Psyr_0079 at 85.690 kb on - strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.741 kb on + strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.742 kb on - strand, within Psyr_0079 at 85.801 kb on + strand, within Psyr_0079 at 85.801 kb on + strand, within Psyr_0079 at 85.801 kb on + strand, within Psyr_0079 at 85.801 kb on + strand, within Psyr_0079 at 85.802 kb on - strand, within Psyr_0079 at 85.802 kb on - strand, within Psyr_0079 at 85.802 kb on - strand, within Psyr_0079 at 85.802 kb on - strand, within Psyr_0079 at 85.802 kb on - strand, within Psyr_0079 at 85.837 kb on + strand, within Psyr_0079 at 85.837 kb on + strand, within Psyr_0079 at 85.837 kb on + strand, within Psyr_0079 at 85.838 kb on - strand, within Psyr_0079 at 85.838 kb on - strand, within Psyr_0079 at 85.838 kb on - strand, within Psyr_0079 at 85.838 kb on - strand, within Psyr_0079 at 85.838 kb on - strand, within Psyr_0079 at 85.838 kb on - strand, within Psyr_0079 at 85.858 kb on + strand, within Psyr_0079 at 85.858 kb on + strand, within Psyr_0079 at 85.858 kb on + strand, within Psyr_0079 at 85.858 kb on + strand, within Psyr_0079 at 85.858 kb on + strand, within Psyr_0079 at 85.859 kb on - strand, within Psyr_0079 at 85.859 kb on - strand, within Psyr_0079 at 85.859 kb on - strand, within Psyr_0079 at 85.859 kb on - strand, within Psyr_0079 at 86.000 kb on + strand, within Psyr_0079 at 86.001 kb on - strand, within Psyr_0079 at 86.001 kb on - strand, within Psyr_0079 at 86.001 kb on - strand, within Psyr_0079 at 86.198 kb on + strand, within Psyr_0079 at 86.198 kb on + strand, within Psyr_0079 at 86.199 kb on - strand, within Psyr_0079 at 86.199 kb on - strand, within Psyr_0079 at 86.199 kb on - strand, within Psyr_0079 at 86.199 kb on - strand, within Psyr_0079 at 86.199 kb on - strand, within Psyr_0079 at 86.236 kb on + strand, within Psyr_0079 at 86.236 kb on + strand, within Psyr_0079 at 86.236 kb on + strand, within Psyr_0079 at 86.236 kb on + strand, within Psyr_0079 at 86.236 kb on + strand, within Psyr_0079 at 86.237 kb on - strand, within Psyr_0079 at 86.237 kb on - strand, within Psyr_0079 at 86.237 kb on - strand, within Psyr_0079 at 86.237 kb on - strand, within Psyr_0079 at 86.237 kb on - strand, within Psyr_0079 at 86.237 kb on - strand, within Psyr_0079 at 86.302 kb on + strand, within Psyr_0079 at 86.303 kb on - strand, within Psyr_0079 at 86.303 kb on - strand, within Psyr_0079 at 86.303 kb on - strand, within Psyr_0079 at 86.303 kb on - strand, within Psyr_0079 at 86.404 kb on + strand at 86.520 kb on + strand at 86.697 kb on - strand, within Psyr_0080 at 86.697 kb on - strand, within Psyr_0080 at 86.697 kb on - strand, within Psyr_0080 at 86.715 kb on + strand, within Psyr_0080 at 86.715 kb on + strand, within Psyr_0080 at 86.716 kb on - strand, within Psyr_0080 at 86.716 kb on - strand, within Psyr_0080 at 86.716 kb on - strand, within Psyr_0080 at 86.798 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.812 kb on + strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.813 kb on - strand, within Psyr_0080 at 86.871 kb on + strand, within Psyr_0080 at 86.871 kb on + strand, within Psyr_0080 at 86.871 kb on + strand, within Psyr_0080 at 86.871 kb on + strand, within Psyr_0080 at 86.871 kb on + strand, within Psyr_0080 at 87.121 kb on + strand, within Psyr_0080 at 87.121 kb on + strand, within Psyr_0080 at 87.122 kb on - strand, within Psyr_0080 at 87.341 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.456 kb on + strand at 87.457 kb on - strand at 87.457 kb on - strand at 87.457 kb on - strand at 87.457 kb on - strand at 87.457 kb on - strand at 87.457 kb on - strand at 87.457 kb on - strand at 87.459 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 84,316 - -1.8 84,357 + +3.7 84,357 + +0.5 84,357 + -1.3 84,357 + +0.5 84,358 - +4.0 84,358 - -3.0 84,358 - +4.1 84,358 - +4.0 84,358 - +0.6 84,358 - -0.5 84,373 + -1.9 84,374 - -1.5 84,374 - -2.0 84,431 - Psyr_0078 0.14 +4.0 84,431 - Psyr_0078 0.14 +1.8 84,431 - Psyr_0078 0.14 +3.2 84,949 + Psyr_0078 0.72 -2.1 84,949 + Psyr_0078 0.72 +1.1 84,949 + Psyr_0078 0.72 -1.1 84,950 - -1.6 84,976 + Psyr_0078 0.75 +0.1 84,976 + Psyr_0078 0.75 -0.0 84,976 + Psyr_0078 0.75 +0.2 84,976 + Psyr_0078 0.75 -1.6 84,976 + Psyr_0078 0.75 +0.2 84,976 + Psyr_0078 0.75 +0.6 84,977 - Psyr_0078 0.75 -2.3 84,977 - Psyr_0078 0.75 +0.2 84,977 - Psyr_0078 0.75 +1.5 84,977 - Psyr_0078 0.75 +4.0 84,977 - Psyr_0078 0.75 +1.8 84,977 - Psyr_0078 0.75 -1.0 84,977 - Psyr_0078 0.75 -0.8 85,355 - Psyr_0079 0.11 -1.1 85,355 - Psyr_0079 0.11 -0.7 85,355 - Psyr_0079 0.11 -0.8 85,358 - Psyr_0079 0.11 +0.4 85,358 - Psyr_0079 0.11 -0.3 85,358 - Psyr_0079 0.11 +2.5 85,358 - Psyr_0079 0.11 +0.2 85,621 + Psyr_0079 0.32 -1.1 85,643 - Psyr_0079 0.34 -0.2 85,690 - Psyr_0079 0.37 -0.5 85,741 + Psyr_0079 0.41 -0.1 85,741 + Psyr_0079 0.41 -0.0 85,741 + Psyr_0079 0.41 -0.3 85,741 + Psyr_0079 0.41 +4.5 85,741 + Psyr_0079 0.41 +0.3 85,741 + Psyr_0079 0.41 +0.2 85,741 + Psyr_0079 0.41 +0.4 85,741 + Psyr_0079 0.41 -1.3 85,741 + Psyr_0079 0.41 +1.9 85,741 + Psyr_0079 0.41 +2.5 85,741 + Psyr_0079 0.41 +1.3 85,741 + Psyr_0079 0.41 +0.5 85,741 + Psyr_0079 0.41 +2.8 85,741 + Psyr_0079 0.41 +3.2 85,741 + Psyr_0079 0.41 +4.3 85,741 + Psyr_0079 0.41 +0.1 85,741 + Psyr_0079 0.41 +3.4 85,741 + Psyr_0079 0.41 -1.3 85,742 - Psyr_0079 0.41 -2.6 85,742 - Psyr_0079 0.41 -0.6 85,742 - Psyr_0079 0.41 +3.0 85,742 - Psyr_0079 0.41 -2.6 85,742 - Psyr_0079 0.41 -1.8 85,742 - Psyr_0079 0.41 +2.2 85,742 - Psyr_0079 0.41 +2.2 85,742 - Psyr_0079 0.41 -2.6 85,742 - Psyr_0079 0.41 -1.2 85,742 - Psyr_0079 0.41 -0.1 85,742 - Psyr_0079 0.41 +0.2 85,742 - Psyr_0079 0.41 -1.7 85,742 - Psyr_0079 0.41 +3.5 85,742 - Psyr_0079 0.41 -1.0 85,742 - Psyr_0079 0.41 +4.1 85,742 - Psyr_0079 0.41 -0.8 85,742 - Psyr_0079 0.41 +4.5 85,801 + Psyr_0079 0.46 +1.3 85,801 + Psyr_0079 0.46 -1.1 85,801 + Psyr_0079 0.46 -1.3 85,801 + Psyr_0079 0.46 -1.4 85,802 - Psyr_0079 0.46 +2.8 85,802 - Psyr_0079 0.46 +1.6 85,802 - Psyr_0079 0.46 -1.0 85,802 - Psyr_0079 0.46 +0.2 85,802 - Psyr_0079 0.46 -0.6 85,837 + Psyr_0079 0.49 +0.0 85,837 + Psyr_0079 0.49 -2.1 85,837 + Psyr_0079 0.49 +2.8 85,838 - Psyr_0079 0.49 -0.7 85,838 - Psyr_0079 0.49 -0.1 85,838 - Psyr_0079 0.49 +1.2 85,838 - Psyr_0079 0.49 +0.9 85,838 - Psyr_0079 0.49 -3.6 85,838 - Psyr_0079 0.49 -2.6 85,858 + Psyr_0079 0.51 -1.5 85,858 + Psyr_0079 0.51 -0.1 85,858 + Psyr_0079 0.51 -2.4 85,858 + Psyr_0079 0.51 -1.7 85,858 + Psyr_0079 0.51 +2.5 85,859 - Psyr_0079 0.51 -1.7 85,859 - Psyr_0079 0.51 -1.9 85,859 - Psyr_0079 0.51 -1.5 85,859 - Psyr_0079 0.51 -0.8 86,000 + Psyr_0079 0.62 -0.6 86,001 - Psyr_0079 0.62 +2.2 86,001 - Psyr_0079 0.62 +3.8 86,001 - Psyr_0079 0.62 -2.0 86,198 + Psyr_0079 0.78 -3.1 86,198 + Psyr_0079 0.78 +1.2 86,199 - Psyr_0079 0.78 -0.5 86,199 - Psyr_0079 0.78 +0.2 86,199 - Psyr_0079 0.78 +2.2 86,199 - Psyr_0079 0.78 +1.3 86,199 - Psyr_0079 0.78 +0.8 86,236 + Psyr_0079 0.81 -1.1 86,236 + Psyr_0079 0.81 -1.3 86,236 + Psyr_0079 0.81 +0.1 86,236 + Psyr_0079 0.81 -1.9 86,236 + Psyr_0079 0.81 +0.3 86,237 - Psyr_0079 0.81 +3.2 86,237 - Psyr_0079 0.81 -0.5 86,237 - Psyr_0079 0.81 -0.2 86,237 - Psyr_0079 0.81 -0.4 86,237 - Psyr_0079 0.81 +1.2 86,237 - Psyr_0079 0.81 -1.2 86,302 + Psyr_0079 0.86 -0.8 86,303 - Psyr_0079 0.86 +0.4 86,303 - Psyr_0079 0.86 -1.4 86,303 - Psyr_0079 0.86 -1.9 86,303 - Psyr_0079 0.86 +2.2 86,404 + +1.4 86,520 + -3.0 86,697 - Psyr_0080 0.28 +0.2 86,697 - Psyr_0080 0.28 +3.9 86,697 - Psyr_0080 0.28 +0.7 86,715 + Psyr_0080 0.30 +1.6 86,715 + Psyr_0080 0.30 +2.5 86,716 - Psyr_0080 0.31 -1.4 86,716 - Psyr_0080 0.31 -3.3 86,716 - Psyr_0080 0.31 +0.2 86,798 + Psyr_0080 0.41 +3.9 86,812 + Psyr_0080 0.42 -1.0 86,812 + Psyr_0080 0.42 +2.2 86,812 + Psyr_0080 0.42 -0.2 86,812 + Psyr_0080 0.42 +3.8 86,812 + Psyr_0080 0.42 +3.4 86,812 + Psyr_0080 0.42 -0.8 86,812 + Psyr_0080 0.42 +0.2 86,812 + Psyr_0080 0.42 +2.2 86,812 + Psyr_0080 0.42 -2.9 86,812 + Psyr_0080 0.42 +3.5 86,812 + Psyr_0080 0.42 -1.3 86,812 + Psyr_0080 0.42 +4.4 86,812 + Psyr_0080 0.42 +5.1 86,812 + Psyr_0080 0.42 -0.4 86,812 + Psyr_0080 0.42 -1.6 86,812 + Psyr_0080 0.42 -0.7 86,812 + Psyr_0080 0.42 +4.0 86,813 - Psyr_0080 0.42 -1.6 86,813 - Psyr_0080 0.42 -0.3 86,813 - Psyr_0080 0.42 +0.4 86,813 - Psyr_0080 0.42 +3.5 86,813 - Psyr_0080 0.42 -0.5 86,813 - Psyr_0080 0.42 +0.0 86,813 - Psyr_0080 0.42 -2.7 86,813 - Psyr_0080 0.42 +0.0 86,813 - Psyr_0080 0.42 +1.2 86,813 - Psyr_0080 0.42 +4.6 86,813 - Psyr_0080 0.42 +3.5 86,813 - Psyr_0080 0.42 -0.3 86,813 - Psyr_0080 0.42 +1.7 86,813 - Psyr_0080 0.42 -0.2 86,813 - Psyr_0080 0.42 +4.2 86,813 - Psyr_0080 0.42 -1.4 86,813 - Psyr_0080 0.42 +0.4 86,813 - Psyr_0080 0.42 +2.5 86,813 - Psyr_0080 0.42 +4.0 86,813 - Psyr_0080 0.42 -1.5 86,813 - Psyr_0080 0.42 -3.4 86,813 - Psyr_0080 0.42 -0.0 86,813 - Psyr_0080 0.42 -1.7 86,813 - Psyr_0080 0.42 +1.9 86,813 - Psyr_0080 0.42 +4.1 86,813 - Psyr_0080 0.42 -1.9 86,813 - Psyr_0080 0.42 -1.9 86,871 + Psyr_0080 0.50 -0.3 86,871 + Psyr_0080 0.50 -2.2 86,871 + Psyr_0080 0.50 +0.8 86,871 + Psyr_0080 0.50 +1.8 86,871 + Psyr_0080 0.50 -1.3 87,121 + Psyr_0080 0.80 -1.5 87,121 + Psyr_0080 0.80 -0.7 87,122 - Psyr_0080 0.80 -1.2 87,341 + -1.6 87,456 + +3.4 87,456 + -0.1 87,456 + +0.6 87,456 + +3.2 87,456 + +0.3 87,456 + -1.1 87,456 + +2.5 87,456 + -0.3 87,456 + -0.2 87,456 + -0.1 87,457 - +0.1 87,457 - -0.7 87,457 - +4.0 87,457 - +1.8 87,457 - +3.2 87,457 - -0.7 87,457 - +2.2 87,459 - +0.2
Or see this region's nucleotide sequence