Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0076 and Psyr_0077 are separated by 591 nucleotides Psyr_0077 and Psyr_0078 are separated by 266 nucleotides
Psyr_0076: Psyr_0076 - Xanthine phosphoribosyltransferase, at 81,563 to 82,132
_0076
Psyr_0077: Psyr_0077 - sigma54 specific transcriptional regulator, Fis family, at 82,724 to 84,043
_0077
Psyr_0078: Psyr_0078 - Beta-lactamase-like protein, at 84,310 to 85,194
_0078
Position (kb)
82
83
84
85 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 81.851 kb on + strand, within Psyr_0076 at 81.851 kb on + strand, within Psyr_0076 at 81.851 kb on + strand, within Psyr_0076 at 81.852 kb on - strand, within Psyr_0076 at 81.852 kb on - strand, within Psyr_0076 at 81.852 kb on - strand, within Psyr_0076 at 82.130 kb on + strand at 82.130 kb on + strand at 82.130 kb on + strand at 82.130 kb on + strand at 82.130 kb on + strand at 82.131 kb on - strand at 82.131 kb on - strand at 82.131 kb on - strand at 82.131 kb on - strand at 82.192 kb on + strand at 82.193 kb on - strand at 82.193 kb on - strand at 82.193 kb on - strand at 82.421 kb on + strand at 82.421 kb on + strand at 82.425 kb on + strand at 82.425 kb on + strand at 82.425 kb on + strand at 82.425 kb on + strand at 82.425 kb on + strand at 82.425 kb on + strand at 82.426 kb on - strand at 82.426 kb on - strand at 82.426 kb on - strand at 82.426 kb on - strand at 82.426 kb on - strand at 82.535 kb on + strand at 82.649 kb on + strand at 82.649 kb on + strand at 82.649 kb on + strand at 82.711 kb on + strand at 82.867 kb on - strand, within Psyr_0077 at 82.898 kb on + strand, within Psyr_0077 at 82.942 kb on + strand, within Psyr_0077 at 82.946 kb on + strand, within Psyr_0077 at 82.947 kb on - strand, within Psyr_0077 at 82.947 kb on - strand, within Psyr_0077 at 82.947 kb on - strand, within Psyr_0077 at 82.947 kb on - strand, within Psyr_0077 at 82.964 kb on + strand, within Psyr_0077 at 82.964 kb on + strand, within Psyr_0077 at 82.964 kb on + strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 82.965 kb on - strand, within Psyr_0077 at 83.001 kb on + strand, within Psyr_0077 at 83.001 kb on + strand, within Psyr_0077 at 83.002 kb on - strand, within Psyr_0077 at 83.002 kb on - strand, within Psyr_0077 at 83.148 kb on + strand, within Psyr_0077 at 83.149 kb on - strand, within Psyr_0077 at 83.149 kb on - strand, within Psyr_0077 at 83.201 kb on - strand, within Psyr_0077 at 83.682 kb on + strand, within Psyr_0077 at 83.682 kb on + strand, within Psyr_0077 at 83.682 kb on + strand, within Psyr_0077 at 83.682 kb on + strand, within Psyr_0077 at 83.943 kb on + strand at 83.943 kb on + strand at 83.944 kb on - strand at 83.988 kb on + strand at 83.989 kb on - strand at 84.190 kb on - strand at 84.316 kb on - strand at 84.357 kb on + strand at 84.357 kb on + strand at 84.357 kb on + strand at 84.357 kb on + strand at 84.358 kb on - strand at 84.358 kb on - strand at 84.358 kb on - strand at 84.358 kb on - strand at 84.358 kb on - strand at 84.358 kb on - strand at 84.373 kb on + strand at 84.374 kb on - strand at 84.374 kb on - strand at 84.431 kb on - strand, within Psyr_0078 at 84.431 kb on - strand, within Psyr_0078 at 84.431 kb on - strand, within Psyr_0078 at 84.949 kb on + strand, within Psyr_0078 at 84.949 kb on + strand, within Psyr_0078 at 84.949 kb on + strand, within Psyr_0078 at 84.950 kb on - strand at 84.976 kb on + strand, within Psyr_0078 at 84.976 kb on + strand, within Psyr_0078 at 84.976 kb on + strand, within Psyr_0078 at 84.976 kb on + strand, within Psyr_0078 at 84.976 kb on + strand, within Psyr_0078 at 84.976 kb on + strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078 at 84.977 kb on - strand, within Psyr_0078
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 81,851 + Psyr_0076 0.51 -0.7 81,851 + Psyr_0076 0.51 -0.5 81,851 + Psyr_0076 0.51 +1.0 81,852 - Psyr_0076 0.51 +4.1 81,852 - Psyr_0076 0.51 -1.2 81,852 - Psyr_0076 0.51 +1.5 82,130 + -4.6 82,130 + -0.3 82,130 + -1.3 82,130 + +1.8 82,130 + -0.8 82,131 - -1.9 82,131 - -2.4 82,131 - +0.5 82,131 - -0.3 82,192 + -1.1 82,193 - +2.5 82,193 - -1.7 82,193 - +1.6 82,421 + -0.9 82,421 + +0.2 82,425 + +0.3 82,425 + +2.5 82,425 + +0.5 82,425 + -0.3 82,425 + -0.9 82,425 + -0.1 82,426 - +0.9 82,426 - -1.5 82,426 - +2.8 82,426 - +1.8 82,426 - +2.8 82,535 + +0.6 82,649 + -0.5 82,649 + +0.5 82,649 + +1.1 82,711 + +3.7 82,867 - Psyr_0077 0.11 +0.5 82,898 + Psyr_0077 0.13 -1.1 82,942 + Psyr_0077 0.17 +1.0 82,946 + Psyr_0077 0.17 +2.2 82,947 - Psyr_0077 0.17 -0.5 82,947 - Psyr_0077 0.17 -1.2 82,947 - Psyr_0077 0.17 -0.8 82,947 - Psyr_0077 0.17 +3.4 82,964 + Psyr_0077 0.18 +3.8 82,964 + Psyr_0077 0.18 -1.0 82,964 + Psyr_0077 0.18 -1.9 82,965 - Psyr_0077 0.18 +1.8 82,965 - Psyr_0077 0.18 -0.4 82,965 - Psyr_0077 0.18 +0.2 82,965 - Psyr_0077 0.18 +1.5 82,965 - Psyr_0077 0.18 -1.4 82,965 - Psyr_0077 0.18 -0.2 82,965 - Psyr_0077 0.18 +1.8 82,965 - Psyr_0077 0.18 +0.1 83,001 + Psyr_0077 0.21 -0.9 83,001 + Psyr_0077 0.21 +0.1 83,002 - Psyr_0077 0.21 -0.9 83,002 - Psyr_0077 0.21 +3.7 83,148 + Psyr_0077 0.32 -0.4 83,149 - Psyr_0077 0.32 +0.1 83,149 - Psyr_0077 0.32 +0.0 83,201 - Psyr_0077 0.36 +1.2 83,682 + Psyr_0077 0.73 +4.7 83,682 + Psyr_0077 0.73 -0.3 83,682 + Psyr_0077 0.73 +0.3 83,682 + Psyr_0077 0.73 -0.2 83,943 + -1.5 83,943 + +0.9 83,944 - +3.9 83,988 + +3.0 83,989 - -0.3 84,190 - -0.6 84,316 - -1.8 84,357 + +3.7 84,357 + +0.5 84,357 + -1.3 84,357 + +0.5 84,358 - +4.0 84,358 - -3.0 84,358 - +4.1 84,358 - +4.0 84,358 - +0.6 84,358 - -0.5 84,373 + -1.9 84,374 - -1.5 84,374 - -2.0 84,431 - Psyr_0078 0.14 +4.0 84,431 - Psyr_0078 0.14 +1.8 84,431 - Psyr_0078 0.14 +3.2 84,949 + Psyr_0078 0.72 -2.1 84,949 + Psyr_0078 0.72 +1.1 84,949 + Psyr_0078 0.72 -1.1 84,950 - -1.6 84,976 + Psyr_0078 0.75 +0.1 84,976 + Psyr_0078 0.75 -0.0 84,976 + Psyr_0078 0.75 +0.2 84,976 + Psyr_0078 0.75 -1.6 84,976 + Psyr_0078 0.75 +0.2 84,976 + Psyr_0078 0.75 +0.6 84,977 - Psyr_0078 0.75 -2.3 84,977 - Psyr_0078 0.75 +0.2 84,977 - Psyr_0078 0.75 +1.5 84,977 - Psyr_0078 0.75 +4.0 84,977 - Psyr_0078 0.75 +1.8 84,977 - Psyr_0078 0.75 -1.0 84,977 - Psyr_0078 0.75 -0.8
Or see this region's nucleotide sequence